Separation of amino acids on Cellulose TLC plates

Separation of amino acids on Cellulose

Chromatographic Conditions

Plate TLC Cellulose 20x10cm
Article number 1.05716
Mobile Phase  


a) 1-butanol/acetone/glacial acetic acid/water 35/35/7/23 (v/v)
b) 1-butanol/acetone/glacial acetic acid/water 35/35/7/23 (v/v) + 1mL Ninhydrin solution
Ninhydrin solution: 7g Ninhydrin in 100mL acetone/1-butanol 1/1 (v/v)

Migration distance each 7cm
Application volume 2 µL
Chamber normal chamber without chamber saturation
Detection heat 1-2min at 110°C, Detection white light

Chromatographic Data

No.   compounds       Concentration         (mg/ml) solvent hRf
1 to 10 amino acid mix L-serine 0.15 water/1-propanol 4/6 26
    L-threonine 0.15 water/1-propanol 4/6 34
    L-alanine 0.1 water/1-propanol 4/6 38
    L-methionine 0.15 water/1-propanol 4/6 58
    L-phenylalanine 0.25 water/1-propanol 4/6 67
    L-leucine 0.10 water/1-propanol 4/6 75

Materials

     
Related Links