Separation of Amino Acids on Cellulose HPTLC plates

Separation of amino acids on Cellulose

Chromatographic Conditions

Plate HPTLC Cellulose 20x10cm
Article number 1. 05786
Mobile Phase (v/v) 1. 1-butanol/acetone/glacial acetic acid/water (35/35/7/23)
2. 1-butanol/acetone/glacial acetic acid/water (35/35/7/23) + 1ml Ninhydrin solution
Ninhydrin solution: 7g Ninhydrin in 100ml acetone/1-butanol (1/1)
Migration distance each 7cm
Application volume 2 µL
Chamber normal chamber without chamber saturation
Detection heat 1-2min at 110°C, Detection white light

Chromatographic Data

No.     Compounds Concentration (mg/ml) Solvent hRf
1 to 10 amino acid mix   L-Serin 0.15 water/1-propanol 4/6 31
      L-Threonin 0.15 water/1-propanol 4/6 40
      L-Alanin 0.1 water/1-propanol 4/6 45
      L-Methionin 0.15 water/1-propanol 4/6 65
      L-Phenylalanin 0.25 water/1-propanol 4/6 72
      L-Leucin 0.10 water/1-propanol 4/6 78

Materials