TLC Analysis of Asparagine and Aspartic acid on HPTLC Silica 60F 254s

TLC Analysis of Asparagine and Aspartic acid on HPTLC Silica 60F 254s

Conditions

plate HPTLC pre-coated plate silica gel 60 F 254s extra pure (suitable for aqueous eluents)
eluent ethanol/buffer solution pH 3 85/15 (v/v)
with addition of 0,1 mol/1 tetramethylammonium chloride
migration distance 5 cm
chamber normal chamber without chamber saturation
sample application volume 300 nL
detection spray reagent: ninhydrin
heating up to 110 C for 5 min in-situ evaluation with TLC/HPTLC scanner (Camag) 500nm

Description

Featured Industry Life Science and Biopharma 
Analysis Note 0
Application No. BOR190918A

Materials

     
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