TLC Analysis of Caffeine in Coffee Samples, Quantification of Analyte Concentration

TLC Analysis of Caffeine in Coffee Samples, Quantification of Analyte Concentration

Description

Analysis Note Abstract: HPTLC (High Performance Thin Layer Chromatography) is a very useful analytical technique with very low sample preparation. Shown in the example: the quantification of caffeine in different coffee samples. The boiled coffee samples
were extracted and filtered with a 0.45 μm syringe filter. The plate was pre-conditioned with the mobile phase. After chromatographic separation the plate was scanned with a TLC-Scanner and subsequently quantified.

Results: Caffeine was detected under UV light (254 nm, hRf = 56, Fig. 1), (quantification information Fig. 2). A triple determination of each sample is possible in parallel.

Conclusion: These results demonstrate that caffeine can be easily quantified by TLC with a three-fold determination and minimal sample preparation.

Materials

     
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