TLC Analysis of Hormones on HPTLC Silica Gel 60 RP-18 WF254s (analyte set 1)

TLC Analysis of Hormones on HPTLC Silica Gel 60 RP-18 WF254s (analyte set 1)

Conditions

plate HPTLC pre-coated plate RP-18 wF254s (1.13124.0001)
eluent [A] acetonitrile; [B] water; (60:40, A:B)
migration distance 5 cm
chamber normal chamber without chamber saturation
sample 0.2 μL
detector Spray reagent: MnC12- slufuric acid heated up to 110°C for 5 min
(in situ evaluation with TLC/HPTLC scanner (Camag) UV 254 nm )

Description

Featured Industry Life Science and Biopharma
suitability application for TLC

Materials

     
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