TLC Analysis of Lactose in different products on HPTLC Silica gel 60 F254

TLC Analysis of Lactose in different products on HPTLC Silica gel 60 F254

Conditions

sample preparation Milk: sample was centrifuged and 1 mL diluted in 50 mL water. Sauce: 1 g sauce diluted in 10 mL water. (Folic acid pill: 1 Folic acid pill dissolved in 10 mL water. Application by spraying 8 mm bands with CAMAG ATS4.)
plate 1.05628 HPTLC Silica gel 60 F254 10 cm x 10 cm (1.05628.0001)
eluent [A] acetonitrile; [B] water; (75:25, A:B) containing 0.1% TFA
migration distance 5 cm
chamber normal chamber without chamber saturation
detector Visible light and scan with TLC Scanner III at 450 nm

Materials

     
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