TLC Analysis Of Plant Extracts on HPTLC Silica gel 60 NH2 F254s

TLC Analysis Of Plant Extracts on HPTLC Silica gel 60 NH2 F254s

Conditions

sample preparation: 2 g plant material extracted for 1 h at 60°C under shaking with 50 ml solvent. Filtration of crude extract with an 0.45 μm syringe filter. Application by CAMAG ATS4 6 mm bandwise.
plate: 1.15647 HPTLC Silica gel 60 RP-18 F 254s MS-grade 20cm x 10 cm (1.151647.0001)
eluent: [A] ethylacetat; [B] formic acid; [C] glacial acid; [D] water; (100:11:11:27, A:B:C) containing 0.1% TFA migration distance: 6 cm
chamber: normal chamber without chamber saturation
sample: 0.150 μL
detector: UV, 254 nm (Identification by coupling the TLC – MS Interface (CAMAG) with the Advion expression) (CMS mass spectrometer -ESI mode. Eluent: Acetonitrile / Water 95:5 +  suitability application for TLC0.1% TFA.) Featured Industry Life Science and Biopharma

Materials

     
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