Separation of plant pigments on Silica gel 60 HPTLC plates

Separation of plant pigments on Silica gel 60

Chromatographic Conditions

Plate HPTLC Silica gel 60 F254, 10x10cm
Article number 1. 05628
Mobile Phase (v/v) n-heptane/1-propanol (9/1)
Migration distance 5cm
Application volume 5 µL
sample preparation milling leafs in a mortar with acetone
Chamber normal chamber without chamber saturation
Detection white light

Chromatographic Data

No.   Compounds Solvent
1 to 6 leafs carotenes acetone
    pheophytin  
    chlorophyll a  
    chlorophyll b  
    lutein  
    violaxanthin  
    neoxanthin  

Materials