TLC Analysis of Proteins on ProteoChrom® HPTLC Silica gel 60 F254s

TLC Analysis of Proteins on ProteoChrom® HPTLC Silica gel 60 F254s

Conditions

plate ProteoChrom® HPTLC Silica gel 60 F254s
mobile phase (v/v) 2-butanol/pyridin/ammonia (25% solution)/water 39/34/10/26 (v/v)
migration distance 5cm
detection white light
chamber normal chamber without chamber saturation
detection 0.5% Ninhydrin solution
  Spray the plate about 20 seconds with ninhydrin solution, and thereafter heat the plate for 1-2 minutes at 110°C.

Chromatographic Data

No. Compounds Concentration Solvent Application volume
    (mg/ml)   µl
1 lysozyme digested 2.0 water/ACN 85/15 7.0
2 myoglobin digested 2.0 water/ACN 85/15 7.0
3 cytochrome c digested 2.0 water/ACN 85/15 7.0
4 ß-casein digested 2.0 water/ACN 85/15 7.0
5 BSA digested 2.0 water/ACN 85/15 7.0

Materials

     
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