Separation of steroids on Silica gel 60 RP-18 HPTLC plates

Separation of steroids on Silica gel 60 RP-18

Chromatographic Conditions

Plate HPTLC Silica gel 60 RP-18 F254s 10x10cm
Article number 1. 13724
Mobile Phase (v/v) methanol/water (60/40)
Migration distance 5cm
Application volume 1 µL
Chamber normal chamber without chamber saturation
Detection 20% perchloric acid in ethanol
spray 20s and heat 5min at 100°C, Detection white light

Chromatographic Data

No. Compounds       Concentration (mg/ml) Solvent hRf
1 chloesterol 1.25 methanol 0
3
stanozolol 1.25 methan0l 1
5 methyl testosterone 1.25 methanol 5
7 reichstein´s S 1.25 methanol 14
9 hydrocortisone 1.25 methanol 26
2, 4, 6 and 8 steroid mixture 1.25 methanol -

Materials