TLC Analysis Of Stevioside and Rebaudioside in Stevia Extract on HPTLC Silica Gel 60 F254s

TLC Analysis Of Stevioside and Rebaudioside in Stevia Extract on HPTLC Silica Gel 60 F254s

Conditions

sample preparation 2 g plant material extracted for 1 h at 60°C under shaking with 50 ml solvent.
Filtration of crude extract with an 0.45 μm syringe filter. Application by CAMAG ATS4 6 mm bandwise.
plate 1.15696 HPTLC Silica gel 60 RP-18 F 254s MS-grade 20cm x 10 cm (1.151696.0001)
eluent [A] ethylacetat; [B] formic acid; [C] glacial acid; [D] water; (100:11:11:27, A:B:C) containing 0.1% TFA
migration distance 5 cm
chamber normal chamber without chamber saturation
detector UV 254 nm, UV 366 nm, (derivatisated with Vanillin-sulfuric acid-reagent and
Anisaldehyde-sulfuric acid-reagent detected with white light)

Description

suitability application for TLC
Featured Industry Life Science and Biopharma

Materials

     
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