TLC Analysys of antioxidants in Itoa orientalis Flacourtiaceae extract on HPTLC Silica gel 60 F254s

TLC Analysys of antioxidants in Itoa orientalis Flacourtiaceae extract on HPTLC Silica gel 60 F254s

Conditions

sample preparation 10 mg dried extract was solved in 10 mL Methanol (1.06018) and
were filtered with a 0.45 μm syringe filter by CAMAG ATS4 6 mm bandwise.
plate 1.15696 HPTLC Silica gel 60 F254s 20 cm x10 cm (1.15696.0001)
eluent [A] ethylacetate; [B] formic acid; [C] glacial acid; [D] water;
(100:11:11:27, A:B:C) containing 0.1% n-Heptane
migration distance 5 cm
chamber normal chamber without chamber saturation
detector 366 nm, visible light (Diphenylboric acid-2-aminoethylester-reagent;
Anisaldehyde-sulfuric acid-reagent;
DPPH-reagent (antioxidative activity) and Molybdatophosphoric acid-reagent)

Description

suitability application for TLC
Featured Industry Chemicals and Industrial Polymers

Materials

     
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