TLC Analysys of Antioxidative Flavonoids in Plant Extracts on Silica Gel 60 F254s

TLC Analysys of Antioxidative Flavonoids in Plant Extracts on Silica Gel 60 F254s

Condition

migration distance   5 cm
chamber normal chamber without chamber saturation
detector 366 nm Diphenylboric acid-2-aminoethylester-reagent for Flavonoids, Vanillin-sulfuric acidreagent e.g. for Phenols, Steroids, Glycoside; DPPH reagent for antioxidants (visible light)
sample preparation Extract samples were filtered with a 0.45 μm syringe filter. Application by CAMAG ATS4 6mm bandwise.
plate 1.15445 HPTLC LiChrospher Silica gel 60 F254s 20 cm x 10 cm (1.15445.0001)
eluent
[A] ethylacetate; [B] formic acid; [C] glacial acid; [D] water; (100:11:11:27, A:B:C) containing 0.1% n-Heptane

Materials

     
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