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TLC and HPTLC MS-Grade Plates

Exceptional Sensitivity with Extremely Low Background Signal

Coupling TLC plates with mass spectrometry (TLC-MS) is a new field of high interest in planar chromatography. One particular advantage of TLC-MS is the flexibility it offers in the choice of mobile phases for separations. In comparison, HPLC-MS coupling does not allow the use of certain mobile phases, such as inorganic buffers.

Several combinations of TLC with MS are possible: Elution-based TLC-MS or desorption based TLC-MS. We offer TLC-MS plates as well as HPTLC-MS on Glass or alumina backing to meet the individual need of our customers.

TLC-MS Coupling Techniques

The techniques for coupling TLC with mass spectrometry can be divided into elution-based, or desorption-based. Both approaches are offline, and are performed after the separation is completed and the plate dried. Sample transfer to the mass spectrometer is fast and typically takes less than one minute.

Elution-based TLC-MS

The analyte on the silica plate is dissolved in a solvent and transferred to the mass spectrometer in the liquid phase.

Elution-based TLC-MS

 

In addition the combination to many other analytical methods are possible including eluting the selected band into vile for further investigation

Desorption-based TLC-MS

The analyte is vaporized from the silica, and transferred to the mass spectrometer in the gas phase. Vaporization techniques include gas beam, ion bombardment, and MALDI (matrix-assisted laser desorption/ionization).

TLC-MALDI-MS

Matrix-assisted laser desorption/ionization (MALDI) is an ionization technique that uses a laser energy absorbing matrix to create ions from large molecules with minimal fragmentation. It has been applied to the analysis of biomolecules and large organic molecules and other macromolecules which tend to be fragile and fragment when ionized by more conventional ionization methods.

Matrix-assisted laser desorption/ionization  Matrix-assisted laser desorption/ionization  Matrix-assisted laser desorption/ionization  Matrix-assisted laser desorption/ionization

DART

DART (Direct Analysis in Real Time) is an atmosphertic pressure ion source that instantaneously ionizes

Direct Analysis in Real Time

 

MS-Grade Plates from a Leader in TLC

Merck KGaA, Darmstadt, Germany, introduced the first pre-coated plates on the market. We were also the first to offer glass plates for coupling planer chromatography with mass spectrometry. Our most recent developments in the field are our MS-grade TLC and HPTLC plates. The separation performance of these products is equivalent to our standard TLC and HPTLC plates. The only difference is that the new plates are much cleaner, and allow trace analysis in the nanogram range. Furthermore, our MS-grade plates offer exceptional sensitivity with extremely low background signal, as verified by the experiments below.

Low Background Signal

The comparison below demonstrates the very low background signal of Merck KGaA, Darmstadt, Germany, MS-grade plates.

a) LC-MS background signal measurement on a HPTLC silica gel 60 F254 standard plate 20 x 10 cm with mobile phase ACN/water (95/5).

LC-MS background signal measurement

b) LC-MS background measurement on a HPTLC silica gel 60 F254 MS-grade plate, 20 x 10 cm with mobile phase ACN/water (95/5).

Enhanced Sensitivity

Figure C depicts the trace measurement of caffeine on Merck KGaA, Darmstadt, Germany, HPTLC-MS grade plate. The results clearly show the exceptional sensitivity of the plate.

c) ESI-MS mass spectrum of caffeine, measured from a 20 nanogram TLC spot. Plate: HPTLC silica gel 60 F254 MS-grade 25 glass plates 20 x 10 cm Sample: 20 ng caffeine (MH+) 195.1 Mobile phase: ACN/water (95/5) + 0.1% formic acid.

ESI-MS mass spectrum of caffeine

TLC-MS Grade Plates

Product
No.
Description Layer Sorbent/
Coating Material
Sorbent
Modification
Layer
Thickness
(µm)/
Format(cm)
Support
Material/
Backing
Comment Size/
Content
1.00933.0001 TLC silica gel 60 F254 MS-grade, 25 glass plates 20 x 20 cm Silica gel 60 F254*1 no 200 / 20x20 glass Elution- and desorption-based approach; Binder: Organic polymer 25 plates
1.51022.0001 TLC silica gel 60 F254 MS-grade, 20 Aluminium sheets*3 5x7.5 Silica gel 60 F254*1 no 100 / 5x7.5 aluminum Elution- and desorption-based approach; Binder: Organic polymer 20 sheets
1.51015.0001 TLC silica gel 60 RP-18 F254s MS-grade, 20 Aluminium sheets*3 5x7.5 Silica gel 60 F254s*2 modified RP-18 modified (aliphatic hydro-carbons) 100 / 5x7.5 aluminum Elution- and desorption-based approach; Binder: Organic polymer 20 plates

*1 F254 Fluorscent indicator
*2 F254s Fluoresent indicator, acid resistant
*3 suitable for TLC-MALDI-MS

HPTLC-MS Grade Plates

Product
No.
Description Layer Sorbent/ Coating Material Sorbent
Modification
Layer
Thickness
(µm)/
Format(cm)
Support
Material/
Backing
Comment Size/
Content
1.00934.0001 HPTLC silica gel 60 F254 MS-grade, 25 glass plates 20 x 10 cm Silica gel 60 F254*1 no 100 /
20 x 10
glass Elution-
and desorption-based approach; Binder:
Organic polymer
20 plates
1.51160.0001 HPTLC silica gel 60 F254 MS-grade for MALDI*3, 20 aluminum foils 5 x 7.5 cm Silica gel 60 F254*1 no 100 /
5 x 7.5
aluminum Elution-
and desorption-based approElution- and desorption-based approach; Binder:
Organic polymerach
20 sheets
1.51161.0001 HPTLC silica gel 60 RP18 F254s MS-grade, 25 glass plates 20 x 10 cm Silica gel 60 F254s*2 modified RP-18 modified (aliphatic hydro-carbons) 100 /
20 x 10
glass Elution-
and desorption-based approach; Binder:
Organic polymer
25 plates

*1 F254 Fluorescent indicator
*2 F254s Fluoresent indicator, acid resistant
*3 suitable for TLC-MALDI-MS