Ascentis® Express Phenyl Hexyl Columns for U/HPLC

Improved reversed phase selectivity for polar aromatics and heterocyclic compounds

The phenyl phase of the Ascentis® Express Phenyl-Hexyl column has unique reversed phase selectivity, particularly for polar aromatics and heterocyclic compounds, which arises from solute interaction with the aromatic ring and its delocalized electrons. The Ascentis® Express Phenyl-Hexyl column is complementary (orthogonal) to both the C18 and RP-Amide Ascentis® Express phases because of this unique aromaticity. Phenyl phases also tend to exhibit good shape selectivity, which may originate from solute multipoint interaction with the planar ring system. More retention and selectivity will often be observed for solutes with aromatic electron-withdrawing groups (e.g. fluorine, nitro, etc.) or with a delocalized heterocyclic ring system such as the benzodiazepine compounds.
The Fused-Core® particle technology of the Ascentis® Express Phenyl-Hexyl  column enables high speed and high resolution separations on both HPLC and UHPLC instruments. The column performs typically 40% more efficiently in comparison to fully porous particulate columns with the same particle size.
 

 

 

 


 

 

Ascentis® Express Phenyl-Hexyl Specifications:
Silica: Type B (High purity silica)
Particle Platform: Superficially porous particles (SPP)
Phase Chemistry: Dimethylphenyl-hexyl
Particle Size: 2.0 µm / 2.7 µm / 5.0 µm
Pore Size: 90 Å
Carbon Load: 6.3% / 7.1% / 5.2%
Surface Area: 120 m²/g / 135 m²/g /90 m²/g
pH Range: 2 - 9
Max Temperature at Low pH: 60 °C
Max Temperature at High pH: 40 °C
Endcapped: Yes

 

Phenyl-Hexyl phase combined with Fused-Core® particle technology for separation of polar aromatics and heterocyclics

The Ascentis® Express Phenyl-Hexyl column is a high-speed, HPLC column based on a superficially porous Fused-Core® particle design.  The Fused-Core® particle provides a thin porous shell of high-purity silica surrounding a solid silica core.  This particle design exhibits very high column efficiency due to the shallow diffusion paths and three different particle sizes are available depending on your specific needs:

Fused-Core® particle size 2 µm

2.0 µm particles

Best Fused-Core UHPLC Column

An optimized solution for high-throughput small molecule analysis

 

Fused-Core® particle size 2.7 µm

2.7 µm particles

Fast HPLC on ANY System

A practical solution that delivers UHPLC performance from any HPLC
 

 

Fused-Core® particle size 5 µm

5.0 µm particles

The Lab Work-Horse Column

True plug and play solution for improving existing 3 or 5 µm fully porous particle HPLC columns

 

HPLC Guard Column

Materials