Ascentis® Express RP-Amide Columns for HPLC and UHPLC

Improved separation of basic compounds

While the Ascentis® Express C8 and C18 provide classic reversed-phase selectivity, the RP-Amide phase provides alternate reversed-phase selectivity:

•Improved peak shape for basic compounds

•100% aqueous compatibility

•LC-MS compatibility

•Fast separation with Fused-Core® technology

The Ascentis® Express RP-Amide HPLC column provides increased selectivity for polar compounds, especially those that can act as a hydrogen bond donor.



Ascentis® Express RP-Amide Specifications:
Silica: Type B (High purity silica)
Particle Platform: Superficially porous particles (SPP)
Phase Chemistry: C16-Amide
Particle Size: 2.0 µm / 2.7 µm / 5.0 µm
Pore Size: 90 Å
Carbon Load: 7.3% / 8.3% / 5.1%
Surface Area: 120 m²/g / 135 m²/g /90 m²/g
pH Range: 2 - 9
Max Temperature at Low pH: 60 °C
Max Temperature at High pH: 40 °C
Endcapped: Yes


RP-Amide phase with Fused-Core® particles for fast separation of basic compounds

Ascentis® Express RP-Amide is a high-speed, high-performance liquid chromatography column based on a superficially porous Fused-Core® particle design.  The Fused-Core® particle provides a thin porous shell of high-purity silica surrounding a solid silica core.  This particle design exhibits very high column efficiency due to the shallow diffusion paths. Three different particle sizes are available for your specific needs:

Fused-Core® particle size 2 µm

2.0 µm particles

Best Fused-Core UHPLC Column

An optimized solution for high-throughput small molecule analysis


Fused-Core® particle size 2.7 µm

2.7 µm particles

Fast HPLC on ANY System

A practical solution that delivers UHPLC performance from any HPLC


Fused-Core® particle size 5 µm

5.0 µm particles

The Lab Work-Horse Column

True plug and play solution for improving existing 3 or 5 µm fully porous particle HPLC columns


HPLC Guard Column