NIOSH 5506 – Product & Materials Guide

Polynuclear Aromatic Hydrocarbon Air Monitoring by HPLC

NIOSH 5506 is used in air monitoring to determine the concentration of polynuclear aromatic hydrocarbons (PAH) found in the air in industrial environments. Known to cause cancer and heightened skin sensitivity to sunlight, polynuclear aromatic hydrocarbons, such as naphthalene and pyrene, enter the body through the inhalation of fumes (or dust) and skin contact. Samples for NIOSH 5506 are collected by drawing a known volume of air through a cassette containing a PTFE filter and a backup sorbent tube. After collection, PAH are extracted from the filter and sorbent tube with acetonitrile and the solutions are analyzed by HPLC with either a UV or fluorescence detector. This method is not applicable to samples that require a different extraction solvent, contain large amounts of highly adsorptive particulate matter (e.g., fly ash or diesel soot), or asphalt fume samples. Our product portfolio contains all the Millipore® filtration products you need for particle monitoring, regardless of your method or application.

Analytical Method: High performance liquid chromatography (HPLC) with UV and fluorescence detection

Learn more about the NIOSH 5506 (Issue 3) method

Materials needed for NIOSH 5506

  • 37 mm, 3 µm PTFE-laminated membrane filter
  • 37 mm cellulose support pad in cassette filter holder (opaque) 
  • Sorbent tube
  • Forceps to handle filter membrane

Products for NIOSH 5506

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