DPX® Tips for Automated Solid Phase Extraction (SPE) with HybridSPE® Technology

DPX® Dispersive Pipette Extraction pipette tips incorporate loosely contained HybridSPE® sorbent material that is mixed with the sample solution to accomplish solid phase extraction. HybridSPE® technology is a simple and generic sample preparation platform designed for the gross level removal of endogenous protein and phospholipid interferences from biological plasma and serum prior to LC-MS or LC-MS/MS analysis.

 

Sample preparation with HybridSPE® DPX® Tips

Figure 1. Sample preparation with HybridSPE® DPX® Tips

 

The unique mixing technique employed provides numerous advantages:

  • Minimal elution solvent volumes
  • Rapid extraction times (less than 3 minutes/sample)
  • High extraction efficiencies
  • Easy to perform extractions
  • Lower costs
  • Higher throughput
  • Minimal training requirements
  • Environmentally friendly
 

HybridSPE® DPX® Tips in action on a Hamilton® liquid handling robot

Figure 2. HybridSPE® DPX® Tips in action on a Hamilton® liquid handling robot

 

In this simple technique, biological plasma or serum is first subjected to protein precipitation via the addition and mixing of acidified acetonitrile. Precipitated proteins are then removed by centrifugation and the resulting supernatant is extracted using the HybridSPE® DPX tip which acts as a chemical filter that specifically targets the removal of endogenous sample phospholipids.

The phospholipid retention mechanism is based on a highly selective Lewis acid-base interaction between the proprietary zirconia ions functionally bonded to the HybridSPE® stationary phase and the phosphate moiety consistent with all phospholipids. The resulting eluent is ready for immediate LC-MS or LC-MS/MS analysis.

 

HybridSPE® Sample Prep Workflow Using DPX® Tips

HybridSPE® Sample Prep Workflow Using DPX® Tips

 

Table 1. What size tips do I need?

HybridSPE-PL Sample and PPT Agent Guidelines
  30 mg tips 50 mg tips
Plasma/serum 30-100 µL 100-300 µL
Precipitating agent 90-300 µL 300-900 µL

 

We offer an array of tips that are compatible with the most common liquid handling platforms and pipettors on the market: Tecan, Hamilton, INTEGRA, and a Universal format as well.

Materials

     
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