Replace Classical Liquid-Liquid Extractions with EXtrelut® NT

SLE: Emulsion-Free Supported-Liquid Extractions

Simplified liquid-liquid extraction using emulsion-free supported-liquid extraction

Classical liquid-liquid extraction (LLE) using a separation funnel is often associated with certain disadvantages: formation of emulsion, poor phase separation, high solvent consumption, low degree of automation and high personnel costs. EXtrelut® NT simplifies liquid-liquid extraction by replacing separation funnels. Using a single step is more efficient and provides solvent, material, and time savings in comparison to classical funnel separation.


Specifications of EXtrelut® NT


Characteristics Specially processed, wide-pore diatomaceous earth with a high pore volume

Chemically inert Naturally occurring product
Capacity limit with aqueous sample EXtrelut® NT1 1 mL without any breakthrough
EXtrelut® NT3 3 mL
EXtrelut® NT20 20 mL
pH range pH 1-10
Uniform batch-to-batch quality


Benefits of Extrelut® NT over LLE

  • Minimal solvent usage
  • Simple method
  • Higher sample capacity and throughput
  • Emulsion free extracts
  • Higher purity, suitable for trace analysis


EXtrelut® NT SLE sorbent is extremely versatile and can be used for biological samples, water analysis, food and beverage, and environmental applications. Any LLE of aqueous samples can be easily replaced with Extrelut® NT supported liquid extraction.

With its easy-to-use working principle a higher recovery and cleaner extraction can be achieved. The aqueous sample is simply applied to the LLE of aqueous samples. It distributes itself in the form of a thin film over the chemically inert matrix and thus acts as a stationary phase.

Subsequently, elution takes place using organic solvents that are non miscible with water, solvents like e.g. diethyl ether, ethyl acetate or halogenated hydrocarbons. All the lipophilic substances are extracted from the aqueous into the organic phase. During this process the aqueous phase remains on the stationary phase. The eluate is free from emulsions and can be evaporated for further analysis.

The capacity of EXtrelut® NT pre-packed columns for aqueous samples are specified by the designation

Significantly smaller samples must be appropriately diluted. If larger volumes are applied, the columns are overloaded; water breaks through into the solvent.

Elution is carried out with 2-3 times the sample volume. The liquid may simply be allowed to run through the column by gravity. The column outlet cannula regulates the solvent flow appropriately.


Important EXtrelut® NT extraction parameters


EXtrelut NT® extraction columns Outlet cannulae Maximum sample volume (mL) Waiting period (min) Recommended elution volume (mL)
EXtrelut® NT1 0.60 x 30 mm 1 5 – 10 6
EXtrelut® NT3 0.60 x 30 mm 3 5 – 10 15
EXtrelut® NT20
0.70 x 30 mm 20 10 – 15 40


  1. In order to prevent water breaking through the sample, don’t overload the column.
  2. Shorter waiting times can affect the recoveries adversely.
  3. The recommended sample volumes must be adhered to. Solutions of smaller volumes must be diluted to give indicated volumes.

Application: HILIC separation of antiepileptic drugs (AEDs) in serum after Extrelut® NT SLE

EXtrelut® NT has been used for quite some time within research, for the sample preparation of urine, whole blood, plasma, serum, gastric juice, liquor, amniotic fluid, feces, animal and plant tissue. Other applications are in the areas of environmental and residue analysis, e.g. the analysis of industrial, domestic and waste water. The fractionated elution of acidic and basic substances (e.g. drugs and their metabolites) from body fluids is also possible.


Determination of antiepileptic drugs (AEDs) in serum



Recoveries [mean values N = 3]
Ethosuximide* 14.1 min 84 ± 7 %
Primidone 19.4 min 100 ± 2 %
a-Methyl-apropylsuccinimide 22.5 min Internal standard
Phenobarbital 26.9 min 96 ± 2 %
Hexobarbital 34.2 min 99 ± 2 %
Carbamazepine 36.4 min 97 ± 1 %
Phenytoin 38.0 min 100 ± 1 %
*Ethosuximide is volatile on evaporation    


EXtrelut® NT pre-packed columns

Description Qty. Cat. No.
EXtrelut® NT1 glass columns for 0.1 to 1 mL sample solution 100 columns 1.15094
EXtrelut® NT3 glass columns for 1 to 3 mL sample solution 50 columns 1.15095
EXtrelut® NT20 polyethylene columns including special outlet cannulae
for up to 20 mL sample solution
25 columns 1.15096


EXtrelut® NT packing material

Description Qty. Cat. No.
EXtrelut® NT bulk packing for preparing large-volume columns 1 kg 1.15092
EXtrelut® NT refill packs for refilling 50 EXtrelut® NT20 columns
(incl. replacement filters)
50 bags 1.15093


EXtrelut® NT accessories

Description Qty. Cat. No.
EXtrelut® NT accessories cannulae .60/30 with Luer tip for EXtrelut® NT1 and
EXtrelut® NT3
100 pieces 1.15373
EXtrelut® NT collection tubes with tapered bottom and
screw cap (normal capacity 15 mL) for EXtrelut® NT1 and EXtrelut® NT3
30 pieces 1.15622
Replacement filter for EXtrelut® NT1 (10 mm Ø) 100 pieces 1.14236
Replacement filter for EXtrelut® NT3 (15 mm Ø)
100 pieces 1.14237
Replacement filter for EXtrelut® NT20 (24 mm Ø) 50 pieces 1.14567