Highest Purity Solvents for UHPLC-MS Analysis

For your highly sensitive UHPLC-MS analyses, how can you reduce noise and additional signals to a minimum? Our new high-end UHPLC-MS solvents raise the standard for low baseline noise and clean mass spectra. Our new range of advanced UHPLC-MS LiChrosolv® solvents have been developed to exceed all expectations, providing rapid and reliable results in both ESI/APCI positive and negative ionization modes.

Thanks to their lowest level of background noise and ion suppression, this quality ensures the optimum ionization efficiency to enable the highest sensitivity. With these features, use of these solvents can also help to extend column lifetime. To ensure that you have confidence in your results, we specify the lowest possible limit of polyethylene glycol (PEG) impurities in all our UHPLC-MS solvents. Our advanced UHPLC-MS LiChrosolv® solvents have been designed to meet the highest requirements of UHPLC-MS in research and quality control, including proteomics and metabolomics as well as environmental, clinical, food or industrial testing applications. A new standard for the unlimited application of ultra- high-performance chromatography has been set.

Benefits of our UHPLC-MS solvents

  • Suitability tested and specified for UHPLC-MS and UHPLC-UV: for analytical flexibility
  • Specified quality in positive and negative ESI and APCI MS for lowest detection limits and confidence in analyses in all important MS modes (test 1)
    • ESI/APCI (+) < 2 ppb
    • ESI/APCI (-) < 10 ppb
  • Lowest impurity profile: for interference-free baselines (test 2)
  • Microfiltration through 0.2 µm filter (test 3)
    • prolonged lifetime of filters and mechanical parts in HPLC systems
    • reduced risk of column clogging
  • Packaged in borosilicate glass bottles: minimized contamination with metal ions
  • Borosilicate bottle with GL45 neck finish, which can directly connect to instruments
  • Lowest levels of trace metal impurities: for minimized metal ion adduct formation
    • up to <5ppb
  • Lowest level of polyethylene glycol (PEG) impurities in our entire UHPLC-MS solvent line up to give you confidence in your results (PEG S/N signal-to-noise-ratio <50)

Detection limit analysis for UHPLC-MS solvents

UHPLC-MS gradient run with Acetonitrile for UHPLC-MS LiChrosolv® shows a clear detection and identification of 1 ppb reserpine, 500 ppt propazine and 4 ppb prednisolone with very low back ground interferences.

Test conditions: UHPLC-MS Gradient run with LiChrosolv® Acetonitrile for UPLC- MS and LiChrosolv® Water for UHPLC-MS from 2% Acetonitrile to 98% Acetonitrile. UHPLC column: Supelco Ascentis® Express C18, 2 µm, 2.1 x 30 mm. MS instrument type: Q-TOF. Detection: ESI (+).

UHPLC-MS Analysis- Limit of detection

Figure 1. UHPLC-MS chromatograms showing that the signals for 500 ppt propazine, 1 ppb reserpine, and 4 ppb prednisolone are all higher than the background signal.

Methanol UHPLC-MS solvent baseline competitor comparison

Comparison of LiChrosolv® Methanol for UHPLC-MS (blue line) with two UHPLC-MS competitor products. LiChrosolv® Methanol for UHPLC-MS shows a flat baseline and by far the lowest impurity profile compared to the competition. Both competitor’s high purity UHPLC-MS products A and B show a baseline drift and significant impurity peaks.

UHPLC-MS solvent competitor analysis

Figure 2. UHPLC-MS chromatograms comparing the baseline of our high purity ® solvents against those of two competitors.

Test conditions: UHPLC-MS Gradient run with LiChrosolv® Methanol for UHPLC-MS and LiChrosolv® Water for UHPLC-MS from 2% Methanol to 98% Methanol. UHPLC column: Supelco Ascentis® Express C18, 2 µm, 2.1 x 30 mm. Detection: ToF-MS (ESI (+)).

Acetonitrile UHPLC-MS solvent particle size competitor comparison

Measurement of particle concentrations in LiChrosolv® acetonitrile UHPLC-MS and a competitor UHPLC-MS grade solvent. The particle concentrations are divided into different particle size ranges. Competitor C displays significantly higher particle concentrations in all analyzed particle size ranges. Low particle concentrations prolong lifetime of filters and mechanical parts in HPLC systems and reduce danger of column clogging. High particle concentrations might also result in detection of impurities.

Test conditions: The solvent samples have been measured five times each by a single particle counting system (RION KS-40BF) based on light diffraction.

Solvent particle concentration

Figure 3. Particle size analysis in LiChrosolv® acetonitrile for UHPLC-MS versus an equivalent competitor UHPLC-MS grade solvent.


Solvent Supplier > 0.2 µm Particles/10 mL > 0.3 µm Particles/10 mL 0.5 µm Particles/10 mL 1 µm Particles/10 mL 2 µm Particles/10 mL
Acetonitrile Merck KGaA, Darmstadt, Germany 2 784 ± 58 828 ± 15 83 ± 7 9 ± 3.2 0.6 ± 0.49
  Competitor C > 12 000 > 12 000 > 12 000 2 054 ± 52 456 ± 19



Related Links