Introducing a New Series of Terpenoid CRM Solutions

Markus Obkircher Head of Reference Materials R&D; Jenna Milliken R&D Senior Scientist; Matthias Nold Global Product Manager Reference Materials
 

CannabisAs described in the Analytix Reporter article "Headspace SPME-GC/MS Analysis of Terpenes in Hops and Cannabis", terpenoids are an important group of natural products in many different fields. They play an important role in spices, aromatherapy, perfumes, phytochemicals and are responsible for the intense scent of cannabis. In addition, some of the medicinal or physiological effects found in cannabis are attributed to specific terpenoids. Since terpenes testing and profiling can be used to better understand the characteristics but even more to identify and classify different strains reliable reference materials are needed.

For this purpose, we developed two terpene mixes specifically required for cannabis testing (CRM40755 and CRM40937) and established more than 60 single component CRM solutions for terpenoids. The concentrations of these mixtures and solutions are typically 2000 μg/mL in methanol.

Find these listed below. We also offer nearly 250 neat standards of isoprenoids, which can be found at SigmaAldrich.com/terpenoids.

Please also find an overview of applications and products suitable for cannabis testing at SigmaAldrich.com/cannabis.

 

Table 1: Components of Cannabis Terpene Mix A CRM40755

# Compound Concentration CAS Number
  Internal Standard 2000 μg/mL N/A
2 α-Pinene 2000 μg/mL 80-56-8
3 Camphene 2000 μg/mL 79-92-5
4 β-Pinene 2000 μg/mL 127-91-3
5 3-Carene 2000 μg/mL 13466-78-9
6 α-Terpinene 2000 μg/mL 99-86-5
7 (R)-(+)-Limonene 2000 μg/mL 5989-27-5
8 γ-Terpinene 2000 μg/mL 99-85-4
9 L-(-)-Fenchone 2000 μg/mL 7787-20-4
10 Fenchol 2000 μg/mL 2217-02-9
11 (1R) (+) Camphor 2000 μg/mL 464-49-3
12 Isoborneol 2000 μg/mL 124-76-5
13 Menthol 2000 μg/mL 89-78-1
14 Citronellol 2000 μg/mL 106-22-9
15 (+)-Pulegone 2000 μg/mL 89-82-7
16 Geranyl acetate 2000 μg/mL 105-87-3
17 α Cedrene 2000 μg/mL 469-61-4
18 α Humulene 2000 μg/mL 6753-98-6
19 Nerolidol* 2000 μg/mL 7212-44-4
20 (+)-Cedrol 2000 μg/mL 77-53-2
21 (-)-α-Bisabolol 2000 μg/mL 23089-26-1

 

Table 2: Components of Cannabis Terpene Mix B CRM40937

# Compound Concentration CAS Number
  Internal Standard 2000 μg/mL N/A
2 β-Pinene 2000 μg/mL 18172-67-3
3 (1S)-(+)-3-Carene 2000 μg/mL 498-15-7
4 p-Cymene 2000 μg/mL 99-87-6
5 Limonene 2000 μg/mL 138-86-3
6 Terpinolene 2000 μg/mL 586-62-9
7 Linalool 2000 μg/mL 78-70-6
8 (1S)-(-)-Camphor 2000 μg/mL 464-48-2
9 (+)-Borneol 2000 μg/mL 464-43-7
10 (-)-α-Terpineol 2000 μg/mL 10482-56-1
11 Geraniol 2000 μg/mL 106-24-1
12 β-Caryophyllene 2000 μg/mL 87-44-5
13 cis-Nerolidol 2000 μg/mL 3790-78-1
14 β-Eudesmol 2000 μg/mL 473-15-4
15 Phytol** 2000 μg/mL 7541-49-3

* Area sum of isomers
**Certified as sum of isomers

GC Conditions Cannabis Terpene Mix A - CRM40755

Column: SLB®-5ms 30 m x 0.25 mm x 0.25 μm
Oven: 60 °C for 3.5 min, 3.5 °C/min to 155 °C, 30 °C/min to 300 °C, hold 5 min
Detector: FID, 310 °C
Injection Volume: 1 µL

Cannabis Terpene Mix A

Peak IDs: see listing in Table 1

 

GC Conditions Cannabis Terpene Mix B - CRM40937

Column: SLB®-5ms; 30 m x 0.25 mm x 0.25 μm df
Oven: 60 °C for 3.5 min, 3.5 °C/min to 155 °C, 30 °C/min to 300 °C, hold 5 min
Detector: FID,310 °C
Injection Volume: 1 µL

Cannabis Terpene Mix B

Peak IDs: see listing in Table 2

 

Materials