Phenolic Calibration Standards for Monitoring Wastewaters

By: Vicki Yearick, AnalytiX Volume 6 Article 3

By Vicki Yearick, Environmental Market Segment

Phenolic compounds are important raw materials for the manufacture of plastic resins, pharmaceuticals, laboratory reagents, dyes, germicidal paints and general disinfectants. Because of the toxicity of many phenolics to both humans and animals, manufacturers using them or materials containing them may be required by local or regional government regulatory agencies to monitor their presence in wastewater discharges. Analysis of phenolic compounds at low levels in waste effluents can be complex, requiring extensive sample preparation followed by gas chromatography (GC). High quality analytical standards are essential for routine daily calibration of the GC instrument.

Sigma-Aldrich, through its Supelco brand, offers high quality analytical phenolic and substituted phenolic standards in the form of neat compounds, single component solutions and mixtures, all of which can be found on our web site, These standards are suitable for monitoring phenolic compounds according to United States Environmental Protection Agency (EPA), the Ontario Ministry of Environment and European Union methodologies.

Table 1 lists a sampling of Supelco brand phenolic calibration mixtures available from Sigma-Aldrich. A Certificate of Analysis is provided with each product. Multi-component solutions are certified for purity, identity and concentration. Each component is guaranteed to be within 0.5% of the stated concentration. If you prefer a custom standard, we can also formulate, test and package phenolic calibration standards according to your specifications, saving you time and freeing your resources to focus on more pressing tasks. To obtain a quote for a custom standard, please contact our Technical Service group at, or use our new on-line Custom Standard request form available at

Cat. No. Brand Description Package Size
48859 Supelco EPA Phenols Mix, varied concentration in methanol:
4-Chloro-3-methyl phenol (2500 μg/mL) 2,4-Dichlorophenol (500 μg/mL) 2,4-Dinitrophenol (1500 μg/mL)
2-Nitrophenol (500 μg/mL) Phenol (500 μg/mL) 4-Nitrophenol (2500 μg/mL)
2-Chlorophenol (500 μg/mL) 2,4-Dimethylphenol (500 μg/mL) 2-Methyl-4,6-dinitrophenol(2500 μg/mL)
Pentachlorophenol (2500 μg/mL) 2,4,6-Trichlorophenol (1500 μg/mL)  
1 mL
47899 Supelco EPA 8040 Phenols Calibration Mix, 500 μg/mL each in isopropanol:
2-sec-Butyl-4,6-dinitrophenol 3-Methylphenol 2,3,5,6-Tetrachlorophenol
2-Chlorophenol 4-Methylphenol 2,3,4-Trichlorophenol
2,4-Dichlorophenol 2-Nitrophenol 2,3,5-Trichlorophenol
2,6-Dichlorophenol 4-Nitrophenol 2,3,6-Trichlorophenol
2,4-Dimethylphenol Pentachlorophenol 2,4,5-Trichlorophenol
2,4-Dinitrophenol Phenol 2,4,6-Trichlorophenol
2-Methyl-4,6-dinitrophenol 2,3,4,5-Tetrachlorophenol 3,4,5-Trichlorophenol
2-Methylphenol 2,3,4,6-Tetrachlorophenol  
1 mL
48130-U Supelco MISA Group 20 Phenols Mix A, 2000 μg/mL each in methanol:
o-Cresol 2,3,4,6-Tetrachlorophenol p-Cresol
2,3,5,6-Tetrachlorophenol 2,6-Dichlorophenol 2,3,4-Trichlorophenol
2,4-Dimethylphenol 2,3,5-Trichlorophenol 4,6-Dinitro-o-cresol
1 mL
48131 Supelco MISA Group 20 Phenols Mix B, 2000 μg/mL each in methanol:
p-Chloro-m-cresol 4-Nitrophenol m-Cresol
Pentachlorophenol 2-Chlorophenol Phenol
2,4-Dichlorophenol 2,3,4,5-Tetrachlorophenol 2,4-Dinitrophenol
1 mL
861253 Supelco Phenols Mix, 2000 μg/mL (except where noted) in 2-propanol:
4-Chloro-3-methylphenol 4-Methylphenol (1000 μg/mL) 2-Chlorophenol
2-Nitrophenol 2,4-Dichlorophenol 4-Nitrophenol
2,6-Dichlorophenol Pentachlorophenol 2,4-Dimethylphenol
Phenol 2,4-Dinitrophenol 2,3,4,6-Tetrachlorophenol
2-Methyl-4,6-dimethylphenol 2,4,5-Trichlorophenol 2-Methylphenol
2,4,6-Trichlorophenol 3-Methylphenol (1000 μg/mL)  
1 mL

Table 1 Phenolic compound standards from Sigma-Aldrich


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