Antifungals in Cell Culture

Antifungal compounds are chemicals that target the integrity of fungal cell wall or cell membrane thereby controlling or eliminating fungal infections. Most antifungals either directly or indirectly increase the porosity of the cell wall or membrane or interfere with the steps in their synthesis. In addition to antibacterials, antifungals have been evolved as critical additives to medium when culturing cells.

Amphotericin B for cell culture

Amphotericin B is also known as Fungizone.

Amphotericin B is most effective against fungi and yeast. It binds to sterols, forming pores in the membrane, and causing small molecules to leak out. Amphotericins are stable in cell culture for 3 days at 37 deg C.
 

Product # Name Form Recommended Concentration
E3789 Amphotericin B low endotoxin Powder 2.5 mg/mL
A2411 Amphotericin B from Streptomyces sp. Solid 2.5 mg/mL
A9528 Amphotericin B solubilized Solubilized powder 5.6 mg/mL
A2942 Amphotericin B solution Liquid 10 ml/L

Nystatin for cell culture

Nystatin is also known as Mycostatin or Fungicidine.

Nystatin, like Amphotericin B, binds to the sterols in fungal cell membrane causing small molecules to leak out. However, compared to Amphotericin B, the concentration window of Nystatin is larger and causes less toxicity to the cells. The water-soluble versions of Nystatin are most suitable to protect sensitive cells against contamination. Nystatins are stable in cell culture for 3 days at 37 deg C.
 

Product # Name Form Recommended Concentration
N6803 Nystatin water soluble Solubilized powder 3-5 mg/mL
N9150 Nystatin Ready made solution Liquid 3-5 mg/mL
N6261 Nystatin Powder 2.5 mg/mL
N4014 Nystatin Powder 50 mg/mL
N1638 Nystatin Suspension Suspension 24 ml/L

Other Antifungals for cell culture

Product # Name Form
A5955 Antibiotic Antimycotic Solution (100×), Stabilized Suspension
T5535 Thiabendazole for plant cell culture Powder
P8556 Pentachloronitrobenzene for plant cell culture Powder

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