[Skip to Content](https://www.sigmaaldrich.com#main-content) [![MilliporeSigma](https://www.sigmaaldrich.com/static/logos/purple/millipore_sigma.svg)](https://www.sigmaaldrich.com/US/en) Products Cart0 USEN Products [Login / Register](https://www.sigmaaldrich.com/oidc-sign-in) [Order Lookup](https://www.sigmaaldrich.com/US/en/order-lookup) [Quick Order](https://www.sigmaaldrich.com/US/en/quick-order) Cart0 [Home](https://www.sigmaaldrich.com/US/en)[3D Cell Culture](https://www.sigmaaldrich.com/US/en/applications/cell-culture-and-cell-culture-analysis/cell-culture-by-technique/3d-cell-culture)Poly-L-Lysine Cell Attachment Protocol # Poly-L-Lysine Cell Attachment Protocol ## Attach Cells to a Surface using Poly-lysine Poly-L-lysine is a nonspecific attachment factor for cells and is useful in promoting cell adhesion to solid substrates by enhancing electrostatic interaction between negatively charged ions of the cell membrane and the culture surface. After absorption to the culture surface, poly-lysine increases the number of positively charged cell binding sites. The following protocol applies to product numbers P4707\*, P4832\*, P7280, P6407, P7405, P9155, P6282, and P5899. Optimal conditions must be determined for each cell line and application. 1. Add 50 mL of sterile tissue culture grade water to 5 mg of poly-lysine. 2. Aseptically coat culture surface with 1.0 mL/25 cm2 (only). Rock gently to ensure even coating of the culture surface. 3. After 5 minutes, remove solution by aspiration and thoroughly rinse surface with sterile tissue culture grade water. 4. Allow to dry at least 2 hours before introducing cells and medium. \*__NOTE__: Step 1 is not necessary for poly-lysine solutions (P4707 and P4832). ## Materials Sorry, an unexpected error has occurred Response not successful: Received status code 500 __Related Articles__ - [Poly-lysine Types for Biomedical Applications](https://www.sigmaaldrich.com/US/en/technical-documents/technical-article/cell-culture-and-cell-culture-analysis/cell-growth-and-maintenance/poly-lysine) - [Cultrex ® 3-D Culture Matrix™ Laminin I Protocol](https://www.sigmaaldrich.com/US/en/technical-documents/protocol/cell-culture-and-cell-culture-analysis/3d-cell-culture/3d-culture-matrix-laminin-i) - [Cultrex® 3-D Culture Matrix™ Rat Collagen I Protocol](https://www.sigmaaldrich.com/US/en/technical-documents/protocol/cell-culture-and-cell-culture-analysis/3d-cell-culture/3d-culture-matrix-rat-collagen-i) - [Cultrex® 3D Spheroid BME Invasion Assay Protocol](https://www.sigmaaldrich.com/US/en/technical-documents/protocol/cell-culture-and-cell-culture-analysis/3d-cell-culture/96-well-3d-spheroid-bme-cell-invasion-assay) - [Cancer Stem Cell Tumorsphere Formation Protocol](https://www.sigmaaldrich.com/US/en/technical-documents/protocol/cell-culture-and-cell-culture-analysis/3d-cell-culture/cancer-stem-cell-tumorsphere-formation) - [Co-Culture of MCF-7 Cells and Human Primary Dermal Fibroblasts](https://www.sigmaaldrich.com/US/en/technical-documents/protocol/cell-culture-and-cell-culture-analysis/3d-cell-culture/co-culture-mcf-7-cells-human-primary-dermal-fibroblasts) - [Corning® Matrigel® Basement Membrane Matrix for 3D Culture *In Vitro*](https://www.sigmaaldrich.com/US/en/technical-documents/protocol/cell-culture-and-cell-culture-analysis/3d-cell-culture/corning-matrigel-basement-membrane-matrix-for-3d-culture-in-vitro) - [Corning® Spheroid Microplates](https://www.sigmaaldrich.com/US/en/technical-documents/protocol/cell-culture-and-cell-culture-analysis/3d-cell-culture/corning-spheroid-microplates) __Related Product Categories__ - [Attachment Factors](https://www.sigmaaldrich.com/US/en/products/cell-culture-and-analysis/cell-culture-supplements-and-reagents/attachment-factors) - [Allergenic Fragrance Testing – Certified Materials](https://www.sigmaaldrich.com/US/en/technical-documents/protocol/analytical-chemistry/calibration-qualification-and-validation/allergenic-fragrance-testing) - [Analysis of Alkylphenols Using New Internal Standards](https://www.sigmaaldrich.com/US/en/technical-documents/protocol/analytical-chemistry/calibration-qualification-and-validation/analysis-of-alkylphenols) - [FAME Standard for Optimizing GC System Performance](https://www.sigmaaldrich.com/US/en/technical-documents/protocol/analytical-chemistry/calibration-qualification-and-validation/fame-standard-for) - [Fast and Reliable Environmental Analysis of Aldehyde and Ketone Air Pollutants](https://www.sigmaaldrich.com/US/en/technical-documents/protocol/analytical-chemistry/calibration-qualification-and-validation/fast-and-reliable) - [Hydrocarbon Oil Index in Samples](https://www.sigmaaldrich.com/US/en/technical-documents/protocol/analytical-chemistry/calibration-qualification-and-validation/hydrocarbon-oil-index) - [Neonicotinoids](https://www.sigmaaldrich.com/US/en/technical-documents/protocol/analytical-chemistry/calibration-qualification-and-validation/neonicotinoids-bee-pesticides) - [New GC-MS Method for Mycotoxin Analysis](https://www.sigmaaldrich.com/US/en/technical-documents/protocol/analytical-chemistry/calibration-qualification-and-validation/new-gc-ms-method-for-mycotoxin-analysis) Top __Sign In To Continue__ To continue reading please sign in or create an account. 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