Chemical Compatibility Guide for MultiScreen® Filter Plates

Ratings are based on 100% or concentrated solutions, unless otherwise indicated. Aromatic hydrocarbons (especially ketones, DMF, DMAC, DMSO, THF, acetonitrile) and chlorinated hydrocarbons will attack both membranes and plastics. A chemical listed as NR may be suitable in low concentrations for short exposures, but this must be determined on an individual assay basis.

Compatibility of MultiScreen®HTS and MultiScreen® Classic Filter Plates with Various Reagents

Plate Material/Membrane Polyolefin
copolymer/PTFE
Acrylic or Classic
Styrene/Durapore®
Acrylic or Styrene/ MCE Barex®/TiO2/
Durapore®, MCE, DEAE
Acrylic or Barex®/
TiO2/Styrene,
Immobilon®-P
Acids
Acetic (5%) R R R R R
Acetic, Glacial R L to NR NR R/L to NR L
Boric R R R R R
Trichloroacetic (<20%) R R R R R
Trichloroacetic (20 to 40%) R R L R/L to NR L
Trichloroacetic (>40%) R L to NR NR L/NR NR
Hydrochloric (0.1 N) R R R R R
Hydrochloric (1 N) R R L to NR R/L to NR R to L
Hydrochloric (>1 N) R R NR R/L to NR R
Hydrofluoric R R (20% max.) NR NR R (20% max.)
Nitric (conc) R L to NR NR NR L
Sulfuric (conc) R L NR L/NR L
Bases
NH4OH (6 N) R L to NR NR L to NR R
NaOH (0.1 N) R R to L L to NR R/L to NR R
NaOH (1 N) R L to NR NR L/NR L
NaOH (>6 N) R NR NR NR NR
Urea R R R (Durapore® only) R
Triethylamine R R R R R
Diethanolamine (≤3 mol) R R R R R
Alcohols
Amyl R R NR R/NR R
Benzyl (1%) R R R R R
Butyl R R R R R
Ethanol (40%) R R NR R R
Ethanol (≥50%) R R NR R/NR R (no filtrate collection)
Isobutyl R R R R R
Isopropyl R R L to NR R/L to NR R
Methanol R R to L NR R/NR R
Triton® X-100 Surfactant (<1%) R R R R R

R = Recommended, no known restrictions, NR = Not recommended, membrane or plate severely attacked by chemical, L = Limited, chemical resistance marginal, short time exposures should be tested individually for application. Dilution with water or other non-solvent will likely enhance compatibility.

  1. Scintillants should only be used with Barex® or SAN plates.
  2. Surfactants are in general readily usable with all MultiScreen® plate types. However, before quantitative fluid transfer to a receiver plate, it is essential that the plate be rinsed with non- surfactant containing fluid (e.g., 100 μL PBS) and then the underdrain be thoroughly blotted prior to adding the material which will ultimately be collected. Triton®-X 100 should not be used with MCE membrane in concentrations greater than 5% (the membrane will appear to lose flow).

Compatibility of MultiScreen®HTS and MultiScreen® Classic Filter Plates with Various Reagents

Plate Material/Membrane Polyolefin
copolymer/PTFE
Acrylic or Classic
Styrene/Durapore®
Acrylic or Styrene/ MCE Barex®/TiO2/
Durapore®, MCE, DEAE
Acrylic or Barex®/
TiO2/Styrene,
Immobilon®-P
Solvents
Acetone R NR NR NR NR
Acetonitrile (no filtrate collection, centrifuge) R NR NR NR L (<35%)
Amyl Acetate R NR NR R/NR R
Carbon Tetrachloride R L to NR L to NR R R
Chloroform R NR NR NR NR
Cyclohexanone R NR to L NR to L R/ NR to L NR to L
DMAC R NR NR NR NR
DMF R NR NR NR NR
DMSO (no filtrate collection) R L (70%) NR NR L (10%) max.
Ethylene Glycol R R R R R
Formaldehyde R R (10% max.) NR R (<40%)/NR R (<40%)
Hexane NR R R R R
Methylene Chloride L NR NR NR NR
MEK R NR NR NR NR
MIBK R NR NR L/NR NR/L
Phenol (5%) R L to NR L to NR R R
Pyridine R R R R R
Scintillants NR NR NR R NR/R(1)
Triethylamine R R R R R
Toluene NR NR to L NR to L R NR to L/R
THF R NR NR L/NR NR/L
Xylene R NR NR R R
Other Organics
Attophos® Reagent R R R R R
Fluorescein R R R R R
Glycerine R R R R R
Hydrogen Peroxide R R NR R (3%) R
Polyethylene Glycol R R R R R
Tween® Surfactant (<5%) (2) R R R R R
Triton® X-100 Surfactant (<1%) R R R R R
Triton® X-100 Surfactant (<5%) R R L R/L R
Inorganic Salts
Carbon Disulfite R NR NR NR NR
Sodium Hypochlorite R R NR R (5%)/ NR R

R = Recommended, no known restrictions, NR = Not recommended, membrane or plate severely attacked by chemical, L = Limited, chemical resistance marginal, short time exposures should be tested individually for application. Dilution with water or other non-solvent will likely enhance compatibility.

  1. Scintillants should only be used with Barex® or SAN plates.
  2. Surfactants are in general readily usable with all MultiScreen® plate types. However, before quantitative fluid transfer to a receiver plate, it is essential that the plate be rinsed with non- surfactant containing fluid (e.g., 100 μL PBS) and then the underdrain be thoroughly blotted prior to adding the material which will ultimately be collected. Triton®-X 100 should not be used with MCE membrane in concentrations greater than 5% (the membrane will appear to lose flow).

Chemical Compatibility Guide for the MultiScreen® Vacuum Manifolds

MultiScreen®HTS and MultiScreen® Resist Manifolds

The following tables outline the solvents that have been evaluated for compatibility with the various components of the MultiScreen®HTS vacuum manifold. In general, the manifold will tolerate the same chemicals as the rest of the system.

However, when using concentrated acids, it is important to rinse through the entire manifold at the end of procedures, especially if collection is done through the manifold into a trap, rather than using the collection trays.

MultiScreen®HTS Vacuum Manifold Components

Component Manifold Base/
Collar Gasket
Frame
Gaskets/Tubing Standard Collar Support Grid Tubing Fittings Droplet Trap
Array
Materials of Construction HDPE/
Polypropylene,
Polyolefin
Silicone Nylon Stainless Steel PP with EPDM or
Fluoroelastomer
Seals
PTFE
Acetone R G G E G E
Acetonitrile E G E E G E
Dimethyl Formamide (DMF) E G R E G E
Dimethyl Sulfoxide (DMSO) E G E E G E
Ethyl Acetate E G E E G E
Ethanol E G G E E E
Formic Acid E G NR G G R
Hexane NR NR R E G E
Hydrochloric Acid (37%) E R NR R R R
Isopropanol E E R E E E
Methanol E E R E E E
Methylene Chloride NR NR R E R E
Sodium Hypochlorite E G NR G G R
Tetrahydrofuran (THF) R NR E E NR E
Toluene NR NR E E R E
Trichloroacetic Acid (TCA) E NR G G R R
Trifluoroacetic Acid (TFA) E NR R R G R

E = Excellent performance, G = Good performance, R = Rinse after contact, NR = Not recommended

MultiScreen® Resist Vacuum Manifold Components

Component Manifold Base
& Deep Well Ring
Gaskets Standard Ring Tubing Inside: Outside Support Grid Control Valve On/Off Valve
Materials of Construction HDPE EPDM Nylon FEP-lined Tygon Stainless Steel   Brass socket
steel case
PP with EPDM seal
Comments       Crimping can alter resistance   Normally no fluid contact  
Acetone R-G G G-E E: NR E E G-E
Acetonitrile E E E E: NR E E G-E
Dimethyl Formamide (DMF) E E R E: NR E E G
Dimethyl Sulfoxide (DMSO) E E E E: R E E G
Ethyl Acetate E E E E: NR E G G-E
Ethanol E E G E: E E E E
Formic Acid E E NR G-E: NR G-E P-R G-E
Hexane R R R R-G: P E E G-E
Hydrochloric Acid E E R-NR E: R      
Isopropanol E E R E: G E E E
Methanol E E R E: G E E E
Methylene Chloride R R R-G E: NR E E P-R
Sodium Hypochlorite E E NR-P E: R G G G
Tetrahydrofuran (THF) R R E E: G E E NR
Toluene R R E E: R E E R
Trichloroacetic Acid (TCA) E E G E: G G-E R R-G
Trifluoroacetic Acid (TFA) E E R E: R R-G P-R G

E = Excellent performance, G = Good performance, R = Rinse after contact, NR = Not recommended

Chemical Compatibility Guide for Fixation and Staining

Millicell® cell culture inserts (single-well, 24-well plates and 96-well plates) are designed to support all fixation, staining and immunostaining procedures in a single device. The 24 and 96-well plates are also automation compatible. The variety of membranes offered in the inserts allows the fixed and stained cells to be visualized by stereoscopic microscopy, phase contrast microscopy, or fluorescent methods.

The majority of staining procedures employ a fixation step first. Fixation is required to stabilize sub-cellular morphology and prevent degradation of antigens during subsequent staining procedures. Consult the following table for chemical compatibility information with common fixative chemicals and stains.

General Considerations

  • If the chemicals are compatible with the membrane but not the polystyrene housing, remove the membrane from the housing before adding the chemical.
  • Unless otherwise stated, the chemicals listed are at maximum concentration. If the plastic housing and/or membranes are not compatible with the maximum concentration, they might be compatible at lower concentration.

Chemical Compatibilty of Fixation and Staining Reagents

Chemical Concentration Polystyrene (PS) housing HA (Mixed Cellulose) membrane CM (PTFE) PCF
(Polycarbonate)
  PET (polyteterep- thalate)
Methanol 90% R NR R R R
Ethanol 95% R R R R R
Glacial Acetic Acid 1% R R R R R
Paraformaldehyde 4% R R R R R
Glutaraldehyde 3% R R R R R
Formaldehyde 4% R R R R R
Formamide 50% R NR R R R
Formamide 100% R NR R R R
HCL 1.0% R R R R R
Osmium tetroxide 1.0% R R R R R
Hematoxylin 100% R R R R R
Eosin 1.0% R R R R R
Tryptan Blue 0.1% R R R R R
Nigrosin 0.2% R R R R R
Toludine Blue 0.3% R R R R R

R = recommended, NR = not recommended

Materials