CRISPR Clone, Control, and Cas9 Essentials

This CRISPR Essentials Tool will help you identify which genome editing vector and format best fit your needs. Use the vector tables to compare and contrast components in a variety of systems. The vector maps and sequence files provide visual references to further assist in choosing the right components for your research.

CRISPR Formats

Bacteria Glycerol Stocks: provided in Terrific Broth supplemented with carbenicllin (100 µg/mL) and 10% glycerol
 

No. of Clones Ordered Techware Volume Product Availability
1-24 Individually labeled vials 500 µl Ships in 2-3 days
25+ 96 well plates 120 µl
Ships in 3-10 business days



Plasmid DNA: provided in 1xTris-EDTA
 

Quantity of DNA ordered Concentration Techware Volume Product Availability– Sanger QuickPick™ Knockout Clones Product Availability– Custom CRISPR Clones
1 µg 20 ng/µl 1-24 clones: individually labeled vials

25+ clones: 96 well plates
50 µl Ships in 3 weeks; additional time for clone quantities > 100 Ships in 4 weeks; additional time for clone quantities > 100
10 µg

25 µg

100 µg
Concentration will be reported with product individually labeled vials Varies depending on concentration Ships in 3-4 weeks; additional time for clone quantities > 25 Ships in 4-5 weeks; additional time for clone quantities > 25



Lentivirus: provided in Dulbecco's Modified Eagle's Medium supplemented with 10% Fetal Bovine Serum and 1% Penicillin/Streptomycin

Lentivirus is provided in individually labeled vials, regardless of quantity of clones ordered.

Volume Titer
106 107 108 109
100 µl 1 x 100 µl
2 x 50 µl
4 x 25 µl
1 x 100 µl
2 x 50 µl
4 x 25 µl
2 x 50 µl
4 x 25 µl
5 x 20 µl
200 µl 1 x 200 µl
2 x 100 µl
4 x 50 µl
8 x 25 µl
1 x 200 µl
2 x 100 µl
4 x 50 µl
8 x 25 µl
4 x 50 µl
8 x 25 µl
10 x 20 µl
1 ml 5 x 200 µl
10 x 100 µl
20 x 50 µl
40 x 25 µl
5 x 200 µl
10 x 100 µl
20 x 50 µl
40 x 25 µl
20 x 50 µl
40 x 25 µl
50 x 20 µl
2 ml 10 x 200 µl
20 x 100 µl
40 x 50 µl
80 x 25 µl
10 x 200 µl
20 x 100 µl
40 x 50 µl
80 x 25 µl
40 x 50 µl
80 x 25 µl
100 x 20 µl
5 ml 25 x 200 µl
50 x 100 µl
100 x 50 µl
200 x 25 µl
25 x 200 µl
50 x 100 µl
100 x 50 µl
200 x 25 µl
100 x 50 µl
200 x 25 µl
N/A



Product Availability - Sanger QuickPick™ Knockout Lentivirus
 

Volume Titer
106 107 108 109
100 µl 4 weeks 4 weeks 6 weeks 6 weeks
200 µl 4 weeks 4 weeks 6 weeks 6 weeks
1 ml 6 weeks 6 weeks 6 weeks 6 weeks
2 ml 6 weeks 6 weeks 6 weeks 6 weeks
5 ml 6 weeks 6 weeks 6 weeks N/A



Product Availability - Custom CRISPR Clones Lentivirus
 

Volume Titer
106 107 108 109
100 µl 6 weeks 6 weeks 8 weeks 8 weeks
200 µl 6 weeks 6 weeks 8 weeks 8 weeks
1 ml 8 weeks 8 weeks 8 weeks 8 weeks
2 ml 8 weeks 8 weeks 8 weeks 8 weeks
5 ml 8 weeks 8 weeks 8 weeks N/A



In Vitro
Transcribed RNA (IVT): provided in Molecular Grade Water (DNase-, RNase-, Protease-free)
 

No. of Clones Ordered Techware Volume & Concentration Product Availability
1-24 Individually labeled vials 20 µl, 200 ng/µl Ships in 5 weeks; allow additional time for clone quantities > 25.
25+ Individually labeled vials 20 µl, 200 ng/µl Ships in 5 weeks; allow additional time for clone quantities > 25.



SygRNA® Synthetic Guide RNA: lyophilized
 

Quantities Techware Product Availability
2 nmol
5 nmol
Individually labeled vials Ships in 3 to 5 business days for standard purification.
HPLC-purified ships in 5 to 7 business days
Custom quantities Individually labeled vials, 96 well plates Please inquire

Pricing Discounts

Product Standard Offering Clone Counts
25 - 100
Clone Counts
101-1000
Sanger QuickPick™ KO Bacteria Glycerol Stocks 500 µl or 120 µl of bacteria glycerol stock 20% discount 40% discount
Sanger QuickPick™ KO Plasmid DNA 1 µg Plasmid DNA at 20 ng/µl 20% discount* 40% discount*
Sanger QuickPick™ KO Lentivirus 1 x 200 µl lentivirus, minimum titer 1e6 TU/ml 20% discount** 40% discount**
Custom CRISPR Plasmid DNA 1 µg Plasmid DNA at 20 ng/µl 15% discount* 30% discount*
Custom CRISPR Lentivirus 1 x 200 µl lentivirus, minimum titer 1e6 TU/ml 15% discount** 30% discount**
Custom CRISPR IVT RNA 20 µl IVT RNA at 200 ng/µl 15% discount 30% discount
Custom CRISPR SygRNA® 2 or 5 µg of lyophilized gRNA 15% discount 30% discount

* Discount does not apply to upcharge for DNA amounts > 1 µg
** Discount does not apply to upcharges for larger volume, higher titer, or additional aliquoting

Vectors

Comprehensive Overview of all CRISPR Vectors
 

Vector
No.
Description Order
Clones
System Cas9/gRNA
Content
Format
Availability
Selection Method
p01 U6-gRNA:
CMV-Cas9-2A-tGFP
p01 clones Mam-
malian
Cas9+gRNA Plasmid Fluorophore (GFP)
p02 U6-gRNA:
CMV-Cas9-2A-tRFP
p02 clones Mam-
malian
Cas9+gRNA Plasmid Fluorophore (RFP)
p04 U6-gRNA:
CMV-eCas9-2a-tGFP
p04 clones Mam-
malian
eCas9+gRNA Plasmid Fluorophore (GFP)
p03 U6-gRNA p03 clones Mam-
malian
gRNA only Plasmid  
p50 (monocot) U6-gRNA:
CMV-Cas9-hyg
(Agrobacterium plasmid)
p50 clones Plant Cas9+gRNA Plasmid Hygromycin
p51 (monocot)U6-gRNA:
CMV-Cas9-neo
(Agrobacterium plasmid)
p51 clones Plant Cas9+gRNA Plasmid Neomycin
p52 (monocot)U6-gRNA:
CMV-Cas9-bar
(Agrobacterium plasmid)
p52 clones Plant Cas9+gRNA Plasmid Bialaphos
p53 (dicot)U6-gRNA:
CMV-Cas9-hyg
(Agrobacterium plasmid)
p53 clones Plant Cas9+gRNA Plasmid Hygromycin
p54 (dicot)U6-gRNA:
CMV-Cas9-neo
(Agrobacterium plasmid)
p54 clones Plant Cas9+gRNA Plasmid Neomycin
p55 (dicot)U6-gRNA:
CMV-Cas9-bar
(Agrobacterium plasmid)
p55 clones Plant Cas9+gRNA Plasmid Bialaphos
p56 (monocot)U6-gRNA:
CMV-Cas9-hyg
(bioballistic plasmid)
p56 clones Plant Cas9+gRNA Plasmid Hygromycin
p57 (monocot)U6-gRNA:
CMV-Cas9-neo
(bioballistic plasmid)
p57 clones Plant Cas9+gRNA Plasmid Neomycin
p58 (monocot)U6-gRNA:
CMV-Cas9-bar
(bioballistic plasmid)
p58 clones Plant Cas9+gRNA Plasmid Bialaphos
p59 (dicot)U6-gRNA:
CMV-Cas9-hyg
(bioballistic plasmid)
p59 clones Plant Cas9+gRNA Plasmid Hygromycin
p60 (dicot)U6-gRNA:
CMV-Cas9-neo
(bioballistic plasmid)
p60 clones Plant Cas9+gRNA Plasmid Neomycin
p61 (dicot)U6-gRNA:
CMV-Cas9-bar
(bioballistic plasmid)
p61 clones Plant Cas9+gRNA Plasmid Bialaphos
p62 (monocot)U6-gRNA:
CMV-Cas9-beta
glucuronidase (Agrobacterium plasmid)
p62 clones Plant Cas9+gRNA Plasmid Beta

Glucuronidase
p63 (dicot)U6-gRNA:
CMV-Cas9-beta glucuronidase
(Agrobacterium plasmid)
p63 clones Plant Cas9+gRNA Plasmid Beta
Glucuronidase
LV01 U6-gRNA:
ef1a-puro-2A-Cas9-2A-tGFP
LV01 clones Lenti-
viral
Cas9+gRNA Plasmid &
Lentivirus
Fluorophore (GFP) & Puromycin
LV02 U6-gRNA:ef1a-puro LV02 clones Lenti-
viral
gRNA only Plasmid &
Lentivirus
Puromycin
LV03 U6-gRNA:ef1a-tGFP LV03 clones Lenti-
viral
gRNA only Plasmid &
Lentivirus
Fluorophore (GFP)
LV04 U6-gRNA:hPGK-puro-2A-tBFP Sanger QP KO clones*
Lenti-
viral
gRNA only Plasmid &
Lentivirus
Fluorophore (BFP) & Puromycin

* Web ordering of Sanger QuickPick™ Clones currently available in US only. Contact CRISPR@sial.com for Sanger QuickPick™ Clone orders outside the US. Please check back for updates about global availability.



Mammalian System Vectors

 

Vector No. Description Order
Clones
Cas9/gRNA
Content
Format
Availability
Other Features
Fluorophore Cas9 type
p01 U6-gRNA:CMV-Cas9-2A-tGFP p01 clones Cas9+gRNA Plasmid DNA GFP S. pyogenes
p02 U6-gRNA:CMV-Cas9-2A-tRFP p02 clones Cas9+gRNA Plasmid DNA RFP S. pyogenes
p04 U6-gRNA:CMV-eCas9-2a-tGFP p04 clones Cas9+gRNA Plasmid DNA GFP enhanced specificity
p03 U6-gRNA p03 clones gRNA only Plasmid DNA    



Lentiviral Vectors
 

Vector
No.
Description Order
Clones
Cas9/gRNA Content Format
Availability
Selection
Method
LV01 U6-gRNA:ef1a-puro-2A-Cas9-2A-tGFP LV01 clones Cas9+gRNA Plasmid DNA &
Lentivirus
Fluorophore (GFP) & Puromycin
LV04 U6-gRNA:hPGK-puro-2A-tBFP Sanger QP KO clones*
gRNA only Plasmid DNA &
Lentivirus
Fluorophore (BFP) & Puromycin
LV03 U6-gRNA:ef1a-tGFP LV03 clones gRNA only Plasmid DNA &
Lentivirus
Fluorophore (GFP)
LV02 U6-gRNA:ef1a-puro LV02 clones gRNA only Plasmid DNA &
Lentivirus
Puromycin

* Web ordering of Sanger QuickPick™ Clones currently available in US only. Contact CRISPR@sial.com for Sanger QuickPick™ Clone orders outside the US. Please check back for updates about global availability.



Plant Vectors
 

Vector
No.
Description Order
Clones
Cas9/gRNA
Content
Format
Availability
Species Plasmid
Type
Selection
p63 (dicot)U6-gRNA:CMV-Cas9-beta glucuronidase (Agrobacterium plasmid) p63 clones Cas9+
gRNA
Plasmid
DNA
Dicot Agro-bacterium Beta
Glucuron-
idase
p55 (dicot)U6-gRNA:CMV-Cas9-bar (Agrobacterium plasmid) p55 clones Cas9+
gRNA
Plasmid
DNA
Dicot Agro-bacterium Biala-
phos
p53 (dicot)U6-gRNA:CMV-Cas9-hyg (Agrobacterium plasmid) p53 clones Cas9+
gRNA
Plasmid
DNA
Dicot Agro-bacterium Hygro-
mycin
p54 (dicot)U6-gRNA:CMV-Cas9-neo (Agrobacterium plasmid) p54 clones Cas9+
gRNA
Plasmid
DNA
Dicot Agro-bacterium Neo-
mycin
p61 (dicot)U6-gRNA:CMV-Cas9-bar (bioballistic plasmid) p61 clones Cas9+
gRNA
Plasmid
DNA
Dicot Bio-Ballistic Biala-
phos
p59 (dicot)U6-gRNA:CMV-Cas9-hyg (bioballistic plasmid) p59 clones Cas9+
gRNA
Plasmid
DNA
Dicot Bio-Ballistic Hygro-
mycin
p60 (dicot)U6-gRNA:CMV-Cas9-neo (bioballistic plasmid) p60 clones Cas9+
gRNA
Plasmid
DNA
Dicot Bio-Ballistic Neo-
mycin
p62 (monocot)U6-gRNA:CMV-Cas9-beta glucuronidase (Agrobacterium plasmid) p62 clones Cas9+
gRNA
Plasmid
DNA
Monocot Agro-bacterium Beta
Glucuron-
idase
p52 (monocot)U6-gRNA:CMV-Cas9-bar (Agrobacterium plasmid) p52 clones Cas9+
gRNA
Plasmid
DNA
Monocot Agro-bacterium Biala-
phos
p50 (monocot) U6-gRNA:CMV-Cas9-hyg (Agrobacterium plasmid) p50 clones Cas9+
gRNA
Plasmid
DNA
Monocot Agro-bacterium Hygro-
mycin
p51 (monocot)U6-gRNA:CMV-Cas9-neo (Agrobacterium plasmid) p51 clones Cas9+
gRNA
Plasmid
DNA
Monocot Agro-bacterium Neo-
mycin
p58 (monocot)U6-gRNA:CMV-Cas9-bar (bioballistic plasmid) p58 clones Cas9+
gRNA
Plasmid
DNA
Monocot Bio-Ballistic Biala-
phos
p56 (monocot)U6-gRNA:CMV-Cas9-hyg (bioballistic plasmid) p56 clones Cas9+
gRNA
Plasmid
DNA
Monocot Bio-Ballistic Hygro-
mycin
p57 (monocot)U6-gRNA:CMV-Cas9-neo (bioballistic plasmid) p57 clones Cas9+
gRNA
Plasmid
DNA
Monocot Bio-Ballistic Neo-
mycin

Cas9 Products

Product No. Description System Format Selection
Method
CAS9P Cas9 plasmid Mammalian Plasmid
DNA
 
CAS9D10AP CRISPR Cas9-D10A Nickase Plasmid Mammalian Plasmid
DNA
 
CAS9D10AGFPP CMV-CAS9D10A-2A-GFP Plasmid Mammalian Plasmid
DNA
Fluorophore
(GFP)
CAS9D10ARFPP CMV-CAS9D10A-2A-RFP Plasmid Mammalian Plasmid
DNA
Fluorophore
(RFP)
CAS9GFPP CMV-CAS9-2A-GFP Plasmid Mammalian Plasmid
DNA
Fluorophore
(GFP)
CAS9RFPP CMV-CAS9-2A-RFP Plasmid Mammalian Plasmid
DNA
Fluorophore
(RFP)
CAS9MRNA Cas9 mRNA Mammalian mRNA  
CAS9D10AMRNA CRISPR Cas9-D10A Nickase mRNA Mammalian mRNA  
CAS9PROT CAS9 Protein Mammalian Protein  
TGEN-CP Cas9 Recombinant protein from
Streptococcus pyogenes
Mammalian Protein  
TGEN-CNP Cas9 Nickase Recombinant protein from
Streptococcus pyogenes
Mammalian Protein  
ESPCAS9PRO ESPCAS9 Protein Mammalian Enhanced
Specificity
Protein
 
DCAS9P300 Sigma CRISPR dCas9-p300 Activator
Expression Plasmid
Mammalian Plasmid
DNA
 
CAS9BST CAS9 Blasticidin Lenti Plasmid Lentiviral Plasmid
DNA
Blasticidin
CAS9NEO Cas9 Neomycin Lenti Plasmid Lentiviral Plasmid
DNA
Neomycin
LVCAS9BST Cas9 Blasticidin Lenti Particles Lentiviral Lentivirus Blasticidin
LVCAS9NEO Cas9 Neomycin Lenti Particles Lentiviral Lentivirus Neomycin

CRISPR Controls

Product No. Description Vector Control Type System Format gRNA Type Selection Method
CRISPR06 CRISPR Universal Non-target Negative Control 1, Cas9+gRNA Plasmid p01 Neg.
Mam-malian Plasmid
DNA
Cas9+gRNA Fluorophore (GFP)
CRISPR07 CRISPR Universal Non-target Negative Control 2, Cas9+gRNA Plasmid p01 Neg. Mam-malian Plasmid
DNA
Cas9+gRNA Fluorophore (GFP)
CRISPR08 CRISPR Universal Non-target Negative Control 3, Cas9+gRNA Plasmid p01 Neg. Mam-malian Plasmid
DNA
Cas9+gRNA Fluorophore (GFP)
CRISPR17 CRISPR Activator Human Oct4 Positive Control Plasmid, gRNA Only Expression Plasmid p03 Pos. Mam-malian Plasmid
DNA
gRNA-only  
CRISPR12 CRISPR-Lenti Universal Non-Target Negative Control 1, Cas9+gRNA Lenti Plasmid (Sigma vector) LV01 Neg. Lenti-viral Plasmid
DNA
Cas9+gRNA Fluorophore (GFP) & Puromycin
CRISPR18 CRISPR-Lenti Universal Non-target Negative Control 1, gRNA Only Lenti Plasmid (Sanger vector) LV04 Neg. Lenti-viral Plasmid
DNA
gRNA-only Fluorophore (BFP) & Puromycin
CRISPR19 CRISPR-Lenti Universal Non-target Negative Control 2, gRNA Only Lenti Plasmid (Sanger vector) LV04 Neg. Lenti-viral Plasmid
DNA
gRNA-only Fluorophore (BFP) & Puromycin
CRISPR20 CRISPR-Lenti Universal Non-target Negative Control 3, gRNA Only Lenti Plasmid (Sanger vector) LV04 Neg. Lenti-viral Plasmid
DNA
gRNA-only Fluorophore (BFP) & Puromycin
CRISPR12V CRISPR-Lenti Universal Non-Target Negative Control 1, Cas9+gRNA Lentiviral Particles (Sigma vector) LV01 Neg. Lenti-viral Lenti-virus Cas9+gRNA Fluorophore (GFP) & Puromycin
CRISPR12H CRISPR-Lenti Universal Non-Target Negative Control 1, Cas9+gRNA Lentiviral Particles, High Titer (Sigma vector) LV01 Neg. Lenti-viral Lenti-virus Cas9+gRNA Fluorophore (GFP) & Puromycin
CRISPR15H Gecko2-Lenti Universal Non-Target Negative Control 1, gRNA Only High Titer Lentiviral Particles (Gecko2 vector) LV02 Neg. Lenti-viral Lenti-virus gRNA only Puromycin
CRISPR18V CRISPR-Lenti Universal Non-target Negative Control 1, gRNA Only Lentiviral Particles (Sanger vector) LV04 Neg. Lenti-viral Lenti-virus gRNA-only Fluorophore (BFP) & Puromycin
CRISPR19V CRISPR-Lenti Universal Non-target Negative Control 2, gRNA Only Lentiviral Particles (Sanger vector) LV04 Neg. Lenti-viral Lenti-virus gRNA-only Fluorophore (BFP) & Puromycin
CRISPR20V CRISPR-Lenti Universal Non-target Negative Control 3, gRNA Only Lentiviral Particles (Sanger vector) LV04 Neg. Lenti-viral Lenti-virus gRNA-only Fluorophore (BFP) & Puromycin
CRISPR01 CRISPR Human EMX1 Positive Control Plasmid, gRNA Only Expression Plasmid, includes Cas9 Expression Plasmid (Sigma vector)) p03 Pos. Mam-malian Plasmid
DNA
gRNA-only  
CRISPR02 CRISPR Nickase Human EMX1 Positive
Control Plasmids (gRNA Only Expression
Plasmids, Includes Cas9-D10A Plasmid)
p03 Pos. Mam-malian Plasmid
DNA
gRNA-only  
CRISPR11 CRISPR-Lenti Human EMX1 Positive Control, Cas9+gRNA Lenti Plasmid (Sigma vector) LV01 Pos. Lenti-viral Plasmid
DNA
Cas9+gRNA Fluorophore (GFP) & Puromycin
CRISPR13 CRISPR-Lenti Human HPRT1 Positive Control, Cas9+gRNA Lenti Plasmid (Sigma vector) LV01 Pos. Lenti-viral Plasmid
DNA
Cas9+gRNA Fluorophore (GFP) & Puromycin
CRISPR21 CRISPR-Lenti Human HPRT1 Positive Control, gRNA Only Lenti Plasmid (Sanger vector) LV04 Pos. Lenti-viral Plasmid
DNA
gRNA-only Fluorophore (BFP) & Puromycin
CRISPR11V CRISPR-Lenti Human EMX1 Positive Control, Cas9+gRNA Lentiviral Particles (Sigma vector) LV01 Pos. Lenti-viral Lenti-virus Cas9+gRNA Fluorophore (GFP) & Puromycin
CRISPR13V CRISPR-Lenti Human HPRT1 Positive Control, Cas9+gRNA Lentiviral Particles (Sigma vector) LV01 Pos. Lenti-viral Lenti-virus Cas9+gRNA Fluorophore (GFP) & Puromycin
CRISPR13H CRISPR-Lenti Human HPRT1 Positive Control, Cas9+gRNA Lentiviral Particles, High Titer (Sigma vector) LV01 Pos. Lenti-viral Lenti-virus Cas9+gRNA Fluorophore (GFP) & Puromycin
CRISPR16H Gecko2-Lenti Human HPRT1 Positive Control, gRNA Only High Titer Lentiviral Particles (Gecko2 vector) LV02 Pos. Lenti-viral Lenti-virus gRNA only Puromycin
CRISPR21V CRISPR-Lenti Human HPRT1 Positive Control, gRNA Only Lentiviral Particles (Sanger vector) LV04 Pos. Lenti-viral Lenti-virus gRNA only Fluorophore (BFP) + Puromycin
CRISPRPL01 CRISPR GUS GAPDH Reporter Control for Monocots p62 Pos. Plant Plasmid
DNA
Cas9+gRNA Beta
Glucuronidase
CRISPRPL02 CRISPR GUS GAPDH Reporter Control for Dicots p63 Pos. Plant Plasmid
DNA
Cas9+gRNA Beta
Glucuronidase
HSOLIGOINT CRISPR Integration Kit - Use to determine baseline for Homology Directed Repair for cell lines N/A          
SHP002 CRISPR & MISSION® Lentiviral Packaging Mix N/A          
T1706 TransIT-CRISPR® Transfection Reagent N/A          

Vector Maps and Sequences

Click on the images below for a larger view.