CRISPR Essentials

This CRISPR Essentials page will help you identify which genome editing vector and format best fit your needs. Use the vector tables to compare and contrast components in a variety of systems. The vector maps and sequence files provide visual references to further assist in choosing the right components for your research.

CRISPR Formats

Bacteria Glycerol Stocks: provided in Terrific Broth supplemented with carbenicllin (100 µg/mL) and 10% glycerol
 

No. of Clones Ordered Techware Volume Product Availability
1-24 Individually labeled vials 500 µL
Ships in 2-3 days
25+ 96 well plates 120 µL
Ships in 3-10 business days



Plasmid DNA: provided in 1xTris-EDTA
 

Quantity of DNA ordered Concentration Techware Volume Product Availability– Sanger QuickPick™ Knockout Clones Product Availability– Custom CRISPR Clones
1 µg 20 ng/µL 1-24 clones: individually labeled vials

25+ clones: 96 well plates
50 µL
Ships in 3 weeks; additional time for clone quantities > 100 Ships in 4 weeks; additional time for clone quantities > 100
10 µg

25 µg

100 µg
Concentration will be reported with product individually labeled vials Varies depending on concentration Ships in 3-4 weeks; additional time for clone quantities > 25 Ships in 4-5 weeks; additional time for clone quantities > 25



Lentivirus: provided in Dulbecco's Modified Eagle's Medium supplemented with 10% Fetal Bovine Serum and 1% Penicillin/Streptomycin

Web ordered lentivirus clones are provided in individually labeled vials. Requests for aliquots in plates should be submitted to CRISPR@sial.com.

Volume Titer
106 107 108 109
100 µL N/A N/A 2 x 50 µL
4 x 25 µL
5 x 20 µL
200 µL 1 x 200 µL
2 x 100 µL
4 x 50 µL
8 x 25 µL
1 x 200 µL
2 x 100 µL
4 x 50 µL
8 x 25 µL
4 x 50 µL
8 x 25 µL
10 x 20 µL
1 mL 5 x 200 µL
10 x 100 µL
20 x 50 µL
40 x 25 µL
5 x 200 µL
10 x 100 µL
20 x 50 µL
40 x 25 µL
20 x 50 µL
40 x 25 µL
50 x 20 µL
2 mL 10 x 200 µL
20 x 100 µL
40 x 50 µL
80 x 25 µL
10 x 200 µL
20 x 100 µL
40 x 50 µL
80 x 25 µL
40 x 50 µL
80 x 25 µL
100 x 20 µL
5 mL 25 x 200 µL
50 x 100 µL
100 x 50 µL
200 x 25 µL
25 x 200 µL
50 x 100 µL
100 x 50 µL
200 x 25 µL
100 x 50 µL
200 x 25 µL
N/A



Product Availability - Sanger QuickPick™ Knockout Lentivirus
 

Volume Titer
106 107 108 109
100 µL
N/A N/A 6 weeks 6 weeks
200 µL
4 weeks 4 weeks 6 weeks 6 weeks
1 mL 6 weeks 6 weeks 6 weeks 6 weeks
2 mL 6 weeks 6 weeks 6 weeks 6 weeks
5 mL 6 weeks 6 weeks 6 weeks N/A



Product Availability - Custom CRISPR Clones Lentivirus
 

Volume Titer
106 107 108 109
100 µL
N/A N/A 8 weeks 8 weeks
200 µL
6 weeks 6 weeks 8 weeks 8 weeks
1 mL 8 weeks 8 weeks 8 weeks 8 weeks
2 mL 8 weeks 8 weeks 8 weeks 8 weeks
5 mL 8 weeks 8 weeks 8 weeks N/A



In Vitro
Transcribed RNA (IVT): provided in Molecular Grade Water (DNase-, RNase-, Protease-free)
 

Techware Volume & Concentration Product Availability
Individually labeled vials 20 µL, 200 ng/µL Ships in 5 weeks; allow additional time for clone quantities > 25.



SygRNA® Synthetic Guide RNA: lyophilized
 

Product Quantities Techware Product Availability
crRNA 2 nmol
5 nmol
Individually labeled vials Ships in 3 to 5 business days for standard purification.
HPLC-purified and modified§ HPLC-purified crRNA ships in 5 to 7 business days
crRNA custom quantities Custom quantities Individually labeled vials or 96 well plates Please inquire
TRACRRNA05N 5 nmol Tube Off the Shelf
Modified§/Custom tracrRNA Custom quantities Tube Please inquire

Web ordering is currently available in select countries. Global web ordering capabilities are in development. To order in countries where the webtool is unavailable, request a crRNA quote

§ Modified tracrRNA is needed when using modified crRNA

SygRNA® synthetic CRISPR two-part system requires both crRNA and tracrRNA to complex with Cas9.

Visit SigmaAldrich.com/SygRNA for more information, including protocols

Pricing Discounts

Product Standard Offering Quantities
25 - 100
Quantities 101-1000
Sanger QuickPick™ KO Bacteria Glycerol Stocks 500 µL or 120 µL of bacteria glycerol stock 20% discount 40% discount
Sanger QuickPick™ KO Plasmid DNA 1 µg Plasmid DNA at 20 ng/µL 20% discount* 40% discount*
Sanger QuickPick™ KO Lentivirus 1 x 200 µL lentivirus, minimum titer 1e6 TU/mL 20% discount** 40% discount**
Custom CRISPR Plasmid DNA 1 µg Plasmid DNA at 20 ng/µL 15% discount* 30% discount*
Custom CRISPR Lentivirus 1 x 200 µL lentivirus, minimum titer 1e6 TU/mL 15% discount** 30% discount**
Custom CRISPR IVT RNA 20 µL IVT RNA at 200 ng/µL 15% discount 30% discount

* Discount does not apply to upcharge for DNA amounts > 1 µg
** Discount does not apply to upcharges for larger volume, higher titer, or additional aliquoting

Vectors

Comprehensive Overview of all CRISPR Vectors
 

Vector
No.
Description Order
Clones
System Cas9/gRNA
Content
Format
Availability
Selection Method
p01 U6-gRNA:
CMV-Cas9-2A-tGFP
p01 clones Mam-
malian
Cas9+gRNA Plasmid Fluorophore (GFP)
p02 U6-gRNA:
CMV-Cas9-2A-tRFP
p02 clones Mam-
malian
Cas9+gRNA Plasmid Fluorophore (RFP)
p04 U6-gRNA:
CMV-eCas9-2a-tGFP
p04 clones Mam-
malian
eCas9+gRNA Plasmid Fluorophore (GFP)
p03 U6-gRNA p03 clones Mam-
malian
gRNA only Plasmid  
p50 (monocot) U6-gRNA:
CMV-Cas9-hyg
(Agrobacterium plasmid)
p50 clones Plant Cas9+gRNA Plasmid Hygromycin
p51 (monocot)U6-gRNA:
CMV-Cas9-neo
(Agrobacterium plasmid)
p51 clones Plant Cas9+gRNA Plasmid Neomycin
p52 (monocot)U6-gRNA:
CMV-Cas9-bar
(Agrobacterium plasmid)
p52 clones Plant Cas9+gRNA Plasmid Bialaphos
p53 (dicot)U6-gRNA:
CMV-Cas9-hyg
(Agrobacterium plasmid)
p53 clones Plant Cas9+gRNA Plasmid Hygromycin
p54 (dicot)U6-gRNA:
CMV-Cas9-neo
(Agrobacterium plasmid)
p54 clones Plant Cas9+gRNA Plasmid Neomycin
p55 (dicot)U6-gRNA:
CMV-Cas9-bar
(Agrobacterium plasmid)
p55 clones Plant Cas9+gRNA Plasmid Bialaphos
p56 (monocot)U6-gRNA:
CMV-Cas9-hyg
(bioballistic plasmid)
p56 clones Plant Cas9+gRNA Plasmid Hygromycin
p57 (monocot)U6-gRNA:
CMV-Cas9-neo
(bioballistic plasmid)
p57 clones Plant Cas9+gRNA Plasmid Neomycin
p58 (monocot)U6-gRNA:
CMV-Cas9-bar
(bioballistic plasmid)
p58 clones Plant Cas9+gRNA Plasmid Bialaphos
p59 (dicot)U6-gRNA:
CMV-Cas9-hyg
(bioballistic plasmid)
p59 clones Plant Cas9+gRNA Plasmid Hygromycin
p60 (dicot)U6-gRNA:
CMV-Cas9-neo
(bioballistic plasmid)
p60 clones Plant Cas9+gRNA Plasmid Neomycin
p61 (dicot)U6-gRNA:
CMV-Cas9-bar
(bioballistic plasmid)
p61 clones Plant Cas9+gRNA Plasmid Bialaphos
p62 (monocot)U6-gRNA:
CMV-Cas9-beta
glucuronidase (Agrobacterium plasmid)
p62 clones Plant Cas9+gRNA Plasmid Beta

Glucuronidase
p63 (dicot)U6-gRNA:
CMV-Cas9-beta glucuronidase
(Agrobacterium plasmid)
p63 clones Plant Cas9+gRNA Plasmid Beta
Glucuronidase
LV01 U6-gRNA:
ef1a-puro-2A-Cas9-2A-tGFP
LV01 clones Lenti-
viral
Cas9+gRNA Plasmid &
Lentivirus
Fluorophore (GFP) & Puromycin
LV05 U6-gRNA:EF1a-Cas9+FLAG-2A-Puro LV05 clones Lenti-
viral
Cas9+gRNA Plasmid & Lentivirus Puromycin
LV06 CRISPRa SAM U6-gRNA:EF1a-Puro  LV06 clones Lentiviral CRISPR activator gRNA Plasmid & Lentivirus Puromycin
LV07 CRISPRa SAM U6-gRNA:EF1a-Zeo LV07 clones Lentiviral CRISPR activator gRNA Plasmid & Lentivirus Zeo
LV02 U6-gRNA:ef1a-puro LV02 clones Lenti-
viral
gRNA only Plasmid &
Lentivirus
Puromycin
LV03 U6-gRNA:ef1a-tGFP LV03 clones Lenti-
viral
gRNA only Plasmid &
Lentivirus
Fluorophore (GFP)
LV04 U6-gRNA:hPGK-puro-2A-tBFP Sanger QP KO clones
Lenti-
viral
gRNA only Plasmid &
Lentivirus
Fluorophore (BFP) & Puromycin

Web ordering is currently available in select countries. Global web ordering capabilities are in development. Please contact CRISPR@sial.com for questions or assistance placing clone orders.

Web ordering is currently available in select countries. For countries where web ordering is not yet available, please fill out the Custom Plant CRISPR Ordering Form



Mammalian System Vectors

 

Vector No. Description Order
Clones
Cas9/gRNA
Content
Format
Availability
Other Features
Fluorophore Cas9 type
p01 U6-gRNA:CMV-Cas9-2A-tGFP p01 clones Cas9+gRNA Plasmid DNA GFP S. pyogenes
p02 U6-gRNA:CMV-Cas9-2A-tRFP p02 clones Cas9+gRNA Plasmid DNA RFP S. pyogenes
p04 U6-gRNA:CMV-eCas9-2a-tGFP p04 clones Cas9+gRNA Plasmid DNA GFP enhanced specificity
p03 U6-gRNA p03 clones gRNA only Plasmid DNA    



Lentiviral Vectors
 

Vector
No.
Description Order
Clones
Cas9/gRNA Content Format
Availability
Selection
Method
LV01 U6-gRNA:ef1a-puro-2A-Cas9-2A-tGFP LV01 clones Cas9+gRNA Plasmid DNA &
Lentivirus
Fluorophore (GFP) & Puromycin
LV05 U6-gRNA:EF1a-Cas9+FLAG-2A-Puro LV05 clones Cas9+gRNA Plasmid & Lentivirus Puromycin
LV06 CRISPRa SAM U6-gRNA:EF1a-Puro LV06 clones CRISPR activator gRNA Plasmid & Lentivirus Puromycin
LV07 CRISPRa SAM U6-gRNA:EF1a-Zeo LV07 clones CRISPR activator gRNA Plasmid & Lentivirus Zeo
LV04 U6-gRNA:hPGK-puro-2A-tBFP Sanger QP KO clones
gRNA only Plasmid DNA &
Lentivirus
Fluorophore (BFP) & Puromycin
LV03 U6-gRNA:ef1a-tGFP LV03 clones gRNA only Plasmid DNA &
Lentivirus
Fluorophore (GFP)
LV02 U6-gRNA:ef1a-puro LV02 clones gRNA only Plasmid DNA &
Lentivirus
Puromycin

Web ordering is currently available in select countries. Global web ordering capabilities are in development. Please contact CRISPR@sial.com for questions or assistance placing clone orders.



Plant Vectors
 

Vector
No.
Description Order
Clones
Cas9/gRNA
Content
Format
Availability
Species Plasmid
Type
Selection
p63 (dicot)U6-gRNA:CMV-Cas9-beta glucuronidase (Agrobacterium plasmid) p63 clones Cas9+
gRNA
Plasmid
DNA
Dicot Agro-bacterium Beta
Glucuron-
idase
p55 (dicot)U6-gRNA:CMV-Cas9-bar (Agrobacterium plasmid) p55 clones Cas9+
gRNA
Plasmid
DNA
Dicot Agro-bacterium Biala-
phos
p53 (dicot)U6-gRNA:CMV-Cas9-hyg (Agrobacterium plasmid) p53 clones Cas9+
gRNA
Plasmid
DNA
Dicot Agro-bacterium Hygro-
mycin
p54 (dicot)U6-gRNA:CMV-Cas9-neo (Agrobacterium plasmid) p54 clones Cas9+
gRNA
Plasmid
DNA
Dicot Agro-bacterium Neo-
mycin
p61 (dicot)U6-gRNA:CMV-Cas9-bar (bioballistic plasmid) p61 clones Cas9+
gRNA
Plasmid
DNA
Dicot Bio-Ballistic Biala-
phos
p59 (dicot)U6-gRNA:CMV-Cas9-hyg (bioballistic plasmid) p59 clones Cas9+
gRNA
Plasmid
DNA
Dicot Bio-Ballistic Hygro-
mycin
p60 (dicot)U6-gRNA:CMV-Cas9-neo (bioballistic plasmid) p60 clones Cas9+
gRNA
Plasmid
DNA
Dicot Bio-Ballistic Neo-
mycin
p62 (monocot)U6-gRNA:CMV-Cas9-beta glucuronidase (Agrobacterium plasmid) p62 clones Cas9+
gRNA
Plasmid
DNA
Monocot Agro-bacterium Beta
Glucuron-
idase
p52 (monocot)U6-gRNA:CMV-Cas9-bar (Agrobacterium plasmid) p52 clones Cas9+
gRNA
Plasmid
DNA
Monocot Agro-bacterium Biala-
phos
p50 (monocot) U6-gRNA:CMV-Cas9-hyg (Agrobacterium plasmid) p50 clones Cas9+
gRNA
Plasmid
DNA
Monocot Agro-bacterium Hygro-
mycin
p51 (monocot)U6-gRNA:CMV-Cas9-neo (Agrobacterium plasmid) p51 clones Cas9+
gRNA
Plasmid
DNA
Monocot Agro-bacterium Neo-
mycin
p58 (monocot)U6-gRNA:CMV-Cas9-bar (bioballistic plasmid) p58 clones Cas9+
gRNA
Plasmid
DNA
Monocot Bio-Ballistic Biala-
phos
p56 (monocot)U6-gRNA:CMV-Cas9-hyg (bioballistic plasmid) p56 clones Cas9+
gRNA
Plasmid
DNA
Monocot Bio-Ballistic Hygro-
mycin
p57 (monocot)U6-gRNA:CMV-Cas9-neo (bioballistic plasmid) p57 clones Cas9+
gRNA
Plasmid
DNA
Monocot Bio-Ballistic Neo-
mycin

Web ordering is currently available in select countries. For countries where web ordering is not yet available, please fill out the Custom Plant CRISPR Ordering Form

Cas9 Products

Click on the product number to order and see additional product details
 

Cas9 Proteins
 

Product No. Description System Format Selection
Method
CAS9PROT CAS9 Protein Mammalian Protein  
ESPCAS9PRO ESPCAS9 Protein Mammalian Enhanced
Specificity
Protein
 
CAS9D10APR Cas9-D10A Nickase Protein Mammalian Protein  
DCAS9PROT dCas9-3XFLAG-Biotin Protein Mammalian Protein  
FNCAS9PROT FnCas9 Protein Mammalian Protein  

 

Cas9 Plasmid and Lentivirus
 

Product No. Description System Format Selection
Method
CAS9P Cas9 plasmid Mammalian Plasmid
DNA
 
CAS9D10AP CRISPR Cas9-D10A Nickase Plasmid Mammalian Plasmid
DNA
 
CAS9D10AGFPP CMV-CAS9D10A-2A-GFP Plasmid Mammalian Plasmid
DNA
Fluorophore
(GFP)
CAS9D10ARFPP CMV-CAS9D10A-2A-RFP Plasmid Mammalian Plasmid
DNA
Fluorophore
(RFP)
CAS9GFPP CMV-CAS9-2A-GFP Plasmid Mammalian Plasmid
DNA
Fluorophore
(GFP)
CAS9RFPP CMV-CAS9-2A-RFP Plasmid Mammalian Plasmid
DNA
Fluorophore
(RFP)
CAS9MRNA Cas9 mRNA Mammalian mRNA  
CAS9D10AMRNA CRISPR Cas9-D10A Nickase mRNA Mammalian mRNA  
DCAS9P300 Sigma CRISPR dCas9-p300 Activator
Expression Plasmid
Mammalian Plasmid
DNA
 
CAS9BST CAS9 Blasticidin Lenti Plasmid Lentiviral Plasmid
DNA
Blasticidin
CAS9NEO Cas9 Neomycin Lenti Plasmid Lentiviral Plasmid
DNA
Neomycin
LVCAS9BST Cas9 Blasticidin Lenti Particles Lentiviral Lentivirus Blasticidin
LVCAS9NEO Cas9 Neomycin Lenti Particles Lentiviral Lentivirus Neomycin

SAM CRISPR Activator Cas9 Products
   

Product No. Description Compatible gRNA vector System Format Selection Method Comment  
SAMHELPERV SAM CRISPRa Helper Construct Kit Lentiviral Transduction Particles LV06 & LV07 Lentiviral Lentivirus Blasticidin & Hygromycin Order compatible
gRNA separately

Kit contains 2
components :
• dCas9-VP64-Blasticidin SAM CRISPRa Helper Construct 1
• MS2-P65-HSF1-Hygromycin SAM CRISPRa Helper Construct 2
            SAMHELPERP   SAM CRISPRa Helper Construct Kit Plasmid DNA LV06 & LV07 Lentiviral Plasmid DNA Blasticidin & Hygromycin Order compatible gRNA separately

Kit contains 2
components :
• dCas9-VP64-Blasticidin SAM CRISPRa Helper Construct 1
• MS2-P65-HSF1-Hygromycin SAM CRISPRa Helper Construct 2
SAMVP64BSTV dCas9-VP64-Blasticidin SAM CRISPRa Helper Construct 1 Lentiviral Transduction Particles     LV06 & LV07 Lentiviral Lentivirus Blasticidin Order compatible
gRNA separately  
Requires MS2-P65-HSF1-Hygromycin SAM CRISPRa Helper Construct 2
SAMVP64BST dCas9-VP64-Blasticidin SAM CRISPRa Helper Construct 1 Plasmid DNA LV06 & LV07 Lentiviral Plasmid DNA Blasticidin Order compatible gRNA separately  
Requires MS2-P65-HSF1-Hygromycin SAM CRISPRa Helper Construct 2
  SAMMS2HYGV   MS2-P65-HSF1-Hygromycin SAM CRISPRa Helper Construct 2 Lentiviral Transduction Particles LV06 & LV07 Lentiviral Lentivirus Hygromycin Order compatible gRNA separately  
Requires dCas9-VP64-Blasticidin SAM CRISPRa Helper Construct 1
            SAMMS2HYG     MS2-P65-HSF1-Hygromycin SAM CRISPRa Helper Construct 2 Plasmid DNA LV06 & LV07 Lentiviral Plasmid DNA Hygromycin Order compatible gRNA separately  
Requires dCas9-VP64-Blasticidin SAM CRISPRa Helper Construct 1

CRISPR Controls

Click on the product number to order and see additional product details
 

Product No. Description Vector Control Type System Format gRNA Type Selection Method
CRISPR06 CRISPR Universal Non-target Negative Control 1, Cas9+gRNA Plasmid p01 Neg.
Mammalian Plasmid
DNA
Cas9+gRNA Fluorophore (GFP)
CRISPR07 CRISPR Universal Non-target Negative Control 2, Cas9+gRNA Plasmid p01 Neg. Mammalian Plasmid
DNA
Cas9+gRNA Fluorophore (GFP)
CRISPR08 CRISPR Universal Non-target Negative Control 3, Cas9+gRNA Plasmid p01 Neg. Mammalian Plasmid
DNA
Cas9+gRNA Fluorophore (GFP)
CRISPR17 CRISPR Activator Human Oct4 Positive Control Plasmid, gRNA Only Expression Plasmid p03 Pos. Mammalian Plasmid
DNA
gRNA only  
CRISPR12 CRISPR-Lenti Universal Non-Target Negative Control 1, Cas9+gRNA Lenti Plasmid (Sigma vector) LV01 Neg. Lentiviral Plasmid
DNA
Cas9+gRNA Fluorophore (GFP) & Puromycin
CRISPR18 CRISPR-Lenti Universal Non-target Negative Control 1, gRNA Only Lenti Plasmid (Sanger vector) LV04 Neg. Lentiviral Plasmid
DNA
gRNA only Fluorophore (BFP) & Puromycin
CRISPR19 CRISPR-Lenti Universal Non-target Negative Control 2, gRNA Only Lenti Plasmid (Sanger vector) LV04 Neg. Lentiviral Plasmid
DNA
gRNA only Fluorophore (BFP) & Puromycin
CRISPR20 CRISPR-Lenti Universal Non-target Negative Control 3, gRNA Only Lenti Plasmid (Sanger vector) LV04 Neg. Lentiviral Plasmid
DNA
gRNA only Fluorophore (BFP) & Puromycin
CRISPR12V CRISPR-Lenti Universal Non-Target Negative Control 1, Cas9+gRNA Lentiviral Particles (Sigma vector) LV01 Neg. Lentiviral Lentivirus Cas9+gRNA Fluorophore (GFP) & Puromycin
CRISPR12H CRISPR-Lenti Universal Non-Target Negative Control 1, Cas9+gRNA Lentiviral Particles, High Titer (Sigma vector) LV01 Neg. Lentiviral Lentivirus Cas9+gRNA Fluorophore (GFP) & Puromycin
CRISPR15H Gecko2-Lenti Universal Non-Target Negative Control 1, gRNA Only High Titer Lentiviral Particles (Gecko2 vector) LV02 Neg. Lentiviral Lentivirus gRNA only Puromycin
CRISPR18V CRISPR-Lenti Universal Non-target Negative Control 1, gRNA Only Lentiviral Particles (Sanger vector) LV04 Neg. Lentiviral Lentivirus gRNA only Fluorophore (BFP) & Puromycin
CRISPR19V CRISPR-Lenti Universal Non-target Negative Control 2, gRNA Only Lentiviral Particles (Sanger vector) LV04 Neg. Lentiviral Lentivirus gRNA only Fluorophore (BFP) & Puromycin
CRISPR20V CRISPR-Lenti Universal Non-target Negative Control 3, gRNA Only Lentiviral Particles (Sanger vector) LV04 Neg. Lentiviral Lentivirus gRNA only Fluorophore (BFP) & Puromycin
CRISPR01 CRISPR Human EMX1 Positive Control Plasmid, gRNA Only Expression Plasmid, includes Cas9 Expression Plasmid (Sigma vector)) p03 Pos. Mammalian Plasmid
DNA
gRNA only  
CRISPR02 CRISPR Nickase Human EMX1 Positive
Control Plasmids (gRNA Only Expression
Plasmids, Includes Cas9-D10A Plasmid)
p03 Pos. Mammalian Plasmid
DNA
gRNA only  
CRISPR11 CRISPR-Lenti Human EMX1 Positive Control, Cas9+gRNA Lenti Plasmid (Sigma vector) LV01 Pos. Lentiviral Plasmid
DNA
Cas9+gRNA Fluorophore (GFP) & Puromycin
CRISPR13 CRISPR-Lenti Human HPRT1 Positive Control, Cas9+gRNA Lenti Plasmid (Sigma vector) LV01 Pos. Lentiviral Plasmid
DNA
Cas9+gRNA Fluorophore (GFP) & Puromycin
CRISPR21 CRISPR-Lenti Human HPRT1 Positive Control, gRNA Only Lenti Plasmid (Sanger vector) LV04 Pos. Lentiviral Plasmid
DNA
gRNA only Fluorophore (BFP) & Puromycin
CRISPR11V CRISPR-Lenti Human EMX1 Positive Control, Cas9+gRNA Lentiviral Particles (Sigma vector) LV01 Pos. Lentiviral Lentivirus Cas9+gRNA Fluorophore (GFP) & Puromycin
CRISPR13V CRISPR-Lenti Human HPRT1 Positive Control, Cas9+gRNA Lentiviral Particles (Sigma vector) LV01 Pos. Lentiviral Lentivirus Cas9+gRNA Fluorophore (GFP) & Puromycin
CRISPR13H CRISPR-Lenti Human HPRT1 Positive Control, Cas9+gRNA Lentiviral Particles, High Titer (Sigma vector) LV01 Pos. Lentiviral Lentivirus Cas9+gRNA Fluorophore (GFP) & Puromycin
CRISPR16H Gecko2-Lenti Human HPRT1 Positive Control, gRNA Only High Titer Lentiviral Particles (Gecko2 vector) LV02 Pos. Lentiviral Lentivirus gRNA only Puromycin
CRISPR21V CRISPR-Lenti Human HPRT1 Positive Control, gRNA Only Lentiviral Particles (Sanger vector) LV04 Pos. Lentiviral Lentivirus gRNA only Fluorophore (BFP) + Puromycin
CRISPRPL01 CRISPR GUS GAPDH Reporter Control for Monocots p62 Pos. Plant Plasmid
DNA
Cas9+gRNA Beta
Glucuronidase
CRISPRPL02 CRISPR GUS GAPDH Reporter Control for Dicots p63 Pos. Plant Plasmid
DNA
Cas9+gRNA Beta
Glucuronidase

CRISPR Companion Products

Product No. Description
HSOLIGOINT CRISPR Integration Kit - Use to determine baseline for Homology Directed Repair for cell lines
SHP002 CRISPR & MISSION® Lentiviral Packaging Mix
T1706 TransIT-CRISPR® Transfection Reagent

Vector Maps and Sequences

Click on the images below for a larger view.