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[Home](https://www.sigmaaldrich.com/US/en)[Advanced Gene Editing](https://www.sigmaaldrich.com/US/en/applications/genomics/advanced-gene-editing)CRISPR Essentials: MISSION® CRISPR gRNAs, Cas9 and Related Products
# CRISPR Essentials: MISSION® CRISPR gRNAs, Cas9 and Related Products
Compare and contrast the features of CRISPR ribonucleoprotein (RNP) products: guide RNA (gRNA) and Cas9 products for in vitro and in vivo CRISPR experiments including knockout/knock-in, activation, and inhibition. Select the best formats for your specific applications from synthetic gRNA, plasmid DNA, lentiviral particles, and proteins.
__Section Overview__
- [CRISPR RNP](https://www.sigmaaldrich.com#rnp)
- [mRNA, Plasmids, and Lentivirus](https://www.sigmaaldrich.com#mrna)
- [CRISPR Controls](https://www.sigmaaldrich.com#control)
- [Non-mammalian CRISPR Systems](https://www.sigmaaldrich.com#non)
- [Vectors](https://www.sigmaaldrich.com#vector)
* * *
## CRISPR RNP
### Single-guide Synthetic RNA
Synthetic CRISPR gRNAs with [guaranteed](https://www.sigmaaldrich.com#guaranteed) activity
ProductDescriptionSpecifications AvailableProduct Availability
[PURedit™ Synthetic gRNA](https://www.sigmaaldrich.com/US/en/configurators/genomics)Choose from our collection of [predesigned guides](https://www.sigmaaldrich.com/US/en/semi-configurators/crisprgrna) optimized for knockout in human and mouse genomes or [design](https://www.milliporesigmabioinfo.com/bioinfo_tools/faces/secured/crispr/crispr.xhtml) and [order](https://www.sigmaaldrich.com/US/en/configurators/genomics) custom sequences.
- Purity >80%
- Enhanced quality
- Stabilizing modifications available
Ships in 7-10 business days
[Standard Synthetic gRNA](https://www.sigmaaldrich.com/US/en/configurators/genomics)Choose from our collection of [predesigned guides](https://www.sigmaaldrich.com/US/en/semi-configurators/crisprgrna) optimized for knockout in human and mouse genomes or [design](https://www.milliporesigmabioinfo.com/bioinfo_tools/faces/secured/crispr/crispr.xhtml) and [order](https://www.sigmaaldrich.com/US/en/configurators/genomics) custom sequences.
- Stabilizing modifications available
- 3 nmol
- SpCas9
Ships in 5-7 business days
### Two-part Synthetic gRNA
ProductDescriptionSpecifications AvailableProduct Availability
[Synthetic crRNA](https://www.sigmaaldrich.com/US/en/configurators/genomics)Choose from our collection of [predesigned guides](https://www.sigmaaldrich.com/US/en/semi-configurators/crisprgrna) optimized for knockout in human and mouse genomes or [design](https://www.milliporesigmabioinfo.com/bioinfo_tools/faces/secured/crispr/crispr.xhtml) and [order](https://www.sigmaaldrich.com/US/en/configurators/genomics) custom sequences.
- HPLC or Desalt purification
- Stabilizing modifications available
- 2 and 5 nmol
- Desalt ships in 3-5 business days
- HPLC ships in 5-7 business days
[TRACRRNA05N](https://www.sigmaaldrich.com/US/en/product/sigma/tracrrna05n)Synthetic SpCas9 tracrRNA
5 nmolReady to ship
[TRACRRNAMOD](https://www.sigmaaldrich.com/US/en/product/sigma/tracrrnamod)Synthetic SpCas9 modified tracrRNA
Pair with modified crRNA
5 nmolReady to ship
CRISPR two-part system requires both crRNA and tracrRNA to complex with Cas9.
### Cas9 Proteins
Product No.ProductDescription/Application
[PECAS9](https://www.sigmaaldrich.com/US/en/product/sigma/pecas9)PURedit™ Cas9PURedit™ SpCas9 with enhanced quality and high efficiency. Engineered for high on-target activity without compromising in specificity. Ideal for preclinical and translational applications including cell-based therapies.
[CAS9PL](https://www.sigmaaldrich.com/US/en/product/sigma/cas9pl)Cas9 PlusHigh efficiency SpCas9 variant that gives both high on-target activity and specificity. Ideal for applications requiring high accuracy.
[CAS9GFPPRO](https://www.sigmaaldrich.com/US/en/product/sigma/cas9gfppro)Wild-type SpCas9-EGFP Fusion
Original Wild-Type Cas9 fused to enhanced green fluorescent protein. Ideal for flow cytometry applications and visualization of transfected RNP complexes. Highly efficient
[ECAS9GFPPR](https://www.sigmaaldrich.com/US/en/product/sigma/ecas9gfppr)
Enhanced specificity SpCas9-EGFP Fusion
Cas9 variant with Improved specificity fused to enhanced green fluorescent protein. Ideal for flow cytometry applications, fluorescence microscopy and for experiments that require high accuracy
[CAS9PROT](https://www.sigmaaldrich.com/US/en/product/sigma/cas9prot)Wild-type *Sp*Cas9Original , wild-type Cas9; simple and economical; efficient in a broad range of gene editing applications
[ESPCAS9PRO](https://www.sigmaaldrich.com/US/en/product/sigma/espcas9pro)Enhanced specificity *Sp*Cas9
Cas9 variant with improved specificity ideal for experiments that require high accuracy
[CAS9D10APR](https://www.sigmaaldrich.com/US/en/product/sigma/cas9d10apr)Cas9D10A NickaseA two-gRNA system with improved specificity and decreased off-target potential for specialized genome-editing applications
[DCAS9PROT](https://www.sigmaaldrich.com/US/en/product/sigma/dcas9prot)dCas9-3XFLAG™ BiotinCatalytically inactive *Sp*Cas9 protein containing a 3XFLAGTM tag and Biotin; ideal for DNA detection and isolation and is a component of the [proxy-CRISPR method](https://www.nature.com/articles/ncomms14958)
[FNCAS9PROT](https://www.sigmaaldrich.com/US/en/product/sigma/fncas9prot)*Fn*Cas9 ProteinIdeal for synthetic biology or cell-free applications
## mRNA, PLASMIDS, AND LENTIVIRUS
### Sanger QuickPick™ Knockout Pre-cloned Guides
ProductDescriptionProduct Availability
[Sanger QuickPick™ Knockout Pre-cloned Guides](https://www.sigmaaldrich.com/US/en/semi-configurators/crispr)Choose [ready-to-use guides](https://www.sigmaaldrich.com/US/en/semi-configurators/crispr) from the [Sanger Whole Genome Knockout Library](https://www.sigmaaldrich.com/US/en/technical-documents/technical-article/genomics/advanced-gene-editing/sanger-crispr-arrayed-library) for human and mouse, pre-cloned into the Sanger vector backbone.Ready to ship
### Cas9 Products
Product No.DescriptionFormat
[CAS9P](https://www.sigmaaldrich.com/US/en/product/sigma/cas9p)*Sp*Cas9 plasmidPlasmid DNA
[CAS9GFPP](https://www.sigmaaldrich.com/US/en/product/sigma/cas9gfpp)*Sp*Cas9 GFP PlasmidPlasmid DNA
[CAS9RFPP](https://www.sigmaaldrich.com/US/en/product/sigma/cas9rfpp)*Sp*Cas9 RFP PlasmidPlasmid DNA
[CAS9GEMP](https://www.sigmaaldrich.com/US/en/product/sigma/cas9gemp)*Sp*Cas9 GFP Geminin PlasmidPlasmid DNA
[CAS9D10AP](https://www.sigmaaldrich.com/US/en/product/sigma/cas9d10ap)*Sp*Cas9-D10A Nickase PlasmidPlasmid DNA
[CAS9D10AGFPP](https://www.sigmaaldrich.com/US/en/product/sigma/cas9d10agfpp)*Sp*Cas9-D10A Nickase GFP PlasmidPlasmid DNA
[CAS9D10ARFPP](https://www.sigmaaldrich.com/US/en/product/sigma/cas9d10arfpp)*Sp*Cas9-D10A Nickase RFP PlasmidPlasmid DNA
[DCAS9P](https://www.sigmaaldrich.com/US/en/product/sigma/dcas9p)*Sp*dCas9 PlasmidPlasmid DNA
[DCAS9P300](https://www.sigmaaldrich.com/US/en/product/sigma/dcas9p300)*Sp*dCas9-p300 Activator
PlasmidPlasmid DNA
[DCAS9P300RFP](https://www.sigmaaldrich.com/US/en/product/sigma/dcas9p300rfp)*Sp*dCas9-p300 RFP Activator
Expression PlasmidPlasmid DNA
[FNCAS9P](https://www.sigmaaldrich.com/US/en/product/sigma/fncas9p)*Fn*Cas9 PlasmidPlasmid DNA
[CAS9MRNA](https://www.sigmaaldrich.com/US/en/product/sigma/cas9mrna)*Sp*Cas9 mRNAmRNA
[CAS9D10AMRNA](https://www.sigmaaldrich.com/US/en/product/sigma/cas9d10amrna)*Sp*Cas9-D10A Nickase mRNAmRNA
[CAS9BST](https://www.sigmaaldrich.com/US/en/product/sigma/cas9bst)*Sp*Cas9 Blasticidin Lenti PlasmidPlasmid DNA
[CAS9HYGROVP](https://www.sigmaaldrich.com/US/en/product/sigma/cas9hygrovp)*Sp*Cas9 Hygromycin Lenti PlasmidPlasmid DNA
[CAS9NEO](https://www.sigmaaldrich.com/US/en/product/sigma/cas9neo)*Sp*Cas9 Neomycin Lenti PlasmidPlasmid DNA
[CAS9ZEOVP](https://www.sigmaaldrich.com/US/en/product/sigma/cas9zeovp)*Sp*Cas9 Zeo Lenti PlasmidPlasmid DNA
[CAS9GFPVP](https://www.sigmaaldrich.com/US/en/product/sigma/cas9gfpvp)*Sp*Cas9 GFP Lenti PlasmidPlasmid DNA
[CAS9RFPVP](https://www.sigmaaldrich.com/US/en/product/sigma/cas9rfpvp)*Sp*Cas9 RFP Lenti PlasmidPlasmid DNA
[ESPCAS9BST](https://www.sigmaaldrich.com/US/en/product/sigma/espcas9bst)High Fidelity *Sp* Cas9 Blasticidin Lenti PlasmidPlasmid DNA
[LVCAS9BST](https://www.sigmaaldrich.com/US/en/product/sigma/lvcas9bst)*Sp*Cas9 Blasticidin Lenti ParticlesLentivirus
[LVCAS9NEO](https://www.sigmaaldrich.com/US/en/product/sigma/lvcas9neo)*Sp*Cas9 Neomycin Lenti ParticlesLentivirus
[CAS9BAC1P](https://www.sigmaaldrich.com/US/en/product/sigma/cas9bac1p)
*Sp*Cas9 Lambda Red Homologous Recombination Plasmid for *E.coli*
Plasmid DNA
### [CRISPR Inhibitor and SAM Activator Cas9 Products](https://www.sigmaaldrich.com/US/en/technical-documents/technical-article/genomics/advanced-gene-editing/crispri-crispra-pooled-lentiviral-screening)
[Learn more](https://www.sigmaaldrich.com/US/en/technical-documents/technical-article/genomics/advanced-gene-editing/crispri-crispra-pooled-lentiviral-screening)
Product No.DescriptionCompatible gRNA vectorSystemFormatSelection MethodComment
[SAMHELPERV](https://www.sigmaaldrich.com/US/en/product/sigma/samhelperv)[](https://www.sigmaaldrich.com/US/en/product/sigma/samhelperv)SAM CRISPRa Helper Construct Kit Lentiviral Transduction ParticlesLV06 & LV07LentiviralLentivirusBlasticidin & Hygromycin
Order compatible gRNA separately
Kit contains 2 components:
- dCas9-VP64-Blasticidin SAM CRISPRa Helper Construct 1
- MS2-P65-HSF1-Hygromycin SAM CRISPRa Helper Construct 2
[](https://www.sigmaaldrich.com)[SAMHELPERP](https://www.sigmaaldrich.com/US/en/product/sigma/samhelperp)SAM CRISPRa Helper Construct Kit Plasmid DNALV06 & LV07LentiviralPlasmid DNABlasticidin & Hygromycin
Order compatible gRNA separately
Kit contains 2 components:
- dCas9-VP64-Blasticidin SAM CRISPRa Helper Construct 1
- MS2-P65-HSF1-Hygromycin SAM CRISPRa Helper Construct 2
[](https://www.sigmaaldrich.com)[SAMVP64BSTV](https://www.sigmaaldrich.com/US/en/product/sigma/samvp64bstv)dCas9-VP64-Blasticidin SAM CRISPRa Helper Construct 1 Lentiviral Transduction ParticlesLV06 & LV07LentiviralLentivirusBlasticidinOrder compatible gRNA separately
Requires MS2-P65-HSF1-Hygromycin SAM CRISPRa Helper Construct 2
[](https://www.sigmaaldrich.com)[SAMVP64BST](https://www.sigmaaldrich.com/US/en/product/sigma/samvp64bst)dCas9-VP64-Blasticidin SAM CRISPRa Helper Construct 1 Plasmid DNALV06 & LV07LentiviralPlasmid DNABlasticidinOrder compatible gRNA separately
Requires MS2-P65-HSF1-Hygromycin SAM CRISPRa Helper Construct 2
[](https://www.sigmaaldrich.com)[SAMMS2HYGV](https://www.sigmaaldrich.com/US/en/product/sigma/samms2hygv)MS2-P65-HSF1-Hygromycin SAM CRISPRa Helper Construct 2 Lentiviral Transduction ParticlesLV06 & LV07LentiviralLentivirusHygromycinOrder compatible gRNA separately
Requires dCas9-VP64-Blasticidin SAM CRISPRa Helper Construct 1
[](https://www.sigmaaldrich.com) [SAMMS2HYG](https://www.sigmaaldrich.com/US/en/product/sigma/samms2hyg)MS2-P65-HSF1-Hygromycin SAM CRISPRa Helper Construct 2 Plasmid DNALV06 & LV07LentiviralPlasmid DNAHygromycinOrder compatible gRNA separately
Requires dCas9-VP64-Blasticidin SAM CRISPRa Helper Construct 1
[CRISPRICON](https://www.sigmaaldrich.com/US/en/product/sigma/crispricon)CRISPRi Control & Effector KitLV17LentiviralLentivirusPuromycin & BFP
- KRAB-dCas9 - Blasticidin CRISPRi Construct Lentiviral Transduction Particles
- CRISPRi RAB1A Puromycin Control Transduction Particles
- CRISPRi Nontargeting Control Puromycin Transduction Particles
[CRISPRI10X](https://www.sigmaaldrich.com/US/en/product/sigma/crispri10x)10X CRISPRi Feature Barcode Optimization Kit LV13 LV14 LV15 LV16 LentiviralLentivirusPuromycin & BFP
- RAB1A And Nontargeting Control With 10x Capture Sequence 1 Stem-Loop Position
- RAB1A And Nontargeting Control With 10x Capture Sequence 1 3 Prime Position
- RAB1A And Nontargeting Control With 10x Capture Sequence 2 Stem-Loop Position
- RAB1A And Nontargeting Control With 10x Capture Sequence 2 3 Prime Position
## CRISPR Controls
### Negative Controls
Product No.DescriptionVectorControl TypeSystemFormatgRNA Type
[CRISPR06](https://www.sigmaaldrich.com/US/en/product/sigma/crispr06)CRISPR Universal Non-target Negative Control 1, Cas9+gRNA Plasmid[p01](https://www.sigmaaldrich.com#p01)Neg.
MammalianPlasmid
DNACas9+gRNA
[CRISPR07](https://www.sigmaaldrich.com/US/en/product/sigma/crispr07)CRISPR Universal Non-target Negative Control 2, Cas9+gRNA Plasmid[p01](https://www.sigmaaldrich.com#p01)Neg.MammalianPlasmid
DNACas9+gRNA
[CRISPR08](https://www.sigmaaldrich.com/US/en/product/sigma/crispr08)CRISPR Universal Non-target Negative Control 3, Cas9+gRNA Plasmid[p01](https://www.sigmaaldrich.com#p01)Neg.MammalianPlasmid
DNACas9+gRNA
[CRISPR17](https://www.sigmaaldrich.com/US/en/product/sigma/crispr17)CRISPR Activator Human Oct4 Positive Control Plasmid, gRNA Only Expression Plasmid[p03](https://www.sigmaaldrich.com#p03)Pos.MammalianPlasmid
DNAgRNA only
[CRISPR12](https://www.sigmaaldrich.com/US/en/product/sigma/crispr12)CRISPR-Lenti Universal Non-Target Negative Control 1, Cas9+gRNA Lenti Plasmid (vector)[LV01](https://www.sigmaaldrich.com#LV01)Neg.LentiviralPlasmid
DNACas9+gRNA
[CRISPR18](https://www.sigmaaldrich.com/US/en/product/sigma/crispr18)CRISPR-Lenti Universal Non-target Negative Control 1, gRNA Only Lenti Plasmid (Sanger vector)[LV04](https://www.sigmaaldrich.com#LV04)Neg.LentiviralPlasmid
DNAgRNA only
[CRISPR19](https://www.sigmaaldrich.com/US/en/product/sigma/crispr19)CRISPR-Lenti Universal Non-target Negative Control 2, gRNA Only Lenti Plasmid (Sanger vector)[LV04](https://www.sigmaaldrich.com#LV04)Neg.LentiviralPlasmid
DNAgRNA only
[CRISPR20](https://www.sigmaaldrich.com/US/en/product/sigma/crispr20)CRISPR-Lenti Universal Non-target Negative Control 3, gRNA Only Lenti Plasmid (Sanger vector)[LV04](https://www.sigmaaldrich.com#LV04)Neg.LentiviralPlasmid
DNAgRNA only
[CRISPR12V](https://www.sigmaaldrich.com/US/en/product/sigma/crispr12v)CRISPR-Lenti Universal Non-Target Negative Control 1, Cas9+gRNA Lentiviral Particles (vector)[LV01](https://www.sigmaaldrich.com#LV01)Neg.LentiviralLentivirusCas9+gRNA
[CRISPR12H](https://www.sigmaaldrich.com/US/en/product/sigma/crispr12h)CRISPR-Lenti Universal Non-Target Negative Control 1, Cas9+gRNA Lentiviral Particles, High Titer (vector)[LV01](https://www.sigmaaldrich.com#LV01)Neg.LentiviralLentivirusCas9+gRNA
[CRISPR15H](https://www.sigmaaldrich.com/US/en/product/sigma/crispr15h)Gecko2-Lenti Universal Non-Target Negative Control 1, gRNA Only High Titer Lentiviral Particles (Gecko2 vector)[LV02](https://www.sigmaaldrich.com#LV02)Neg.LentiviralLentivirusgRNA only
[CRISPR18V](https://www.sigmaaldrich.com/US/en/product/sigma/crispr18v)CRISPR-Lenti Universal Non-target Negative Control 1, gRNA Only Lentiviral Particles (Sanger vector)[LV04](https://www.sigmaaldrich.com#LV04)Neg.LentiviralLentivirusgRNA only
[CRISPR19V](https://www.sigmaaldrich.com/US/en/product/sigma/crispr19v)CRISPR-Lenti Universal Non-target Negative Control 2, gRNA Only Lentiviral Particles (Sanger vector)[LV04](https://www.sigmaaldrich.com#LV04)Neg.LentiviralLentivirusgRNA only
[CRISPR20V](https://www.sigmaaldrich.com/US/en/product/sigma/crispr20v)CRISPR-Lenti Universal Non-target Negative Control 3, gRNA Only Lentiviral Particles (Sanger vector)[LV04](https://www.sigmaaldrich.com#LV04)Neg.LentiviralLentivirusgRNA only
[CRISPR01](https://www.sigmaaldrich.com/US/en/product/sigma/crispr01)CRISPR Human EMX1 Positive Control Plasmid, gRNA Only Expression Plasmid, includes Cas9 Expression Plasmid (vector)[p03](https://www.sigmaaldrich.com#p03)Pos.MammalianPlasmid
DNAgRNA only
[CRISPR02](https://www.sigmaaldrich.com/US/en/product/sigma/crispr02)CRISPR Nickase Human EMX1 Positive
Control Plasmids (gRNA Only Expression
Plasmids, Includes Cas9-D10A Plasmid)[p03](https://www.sigmaaldrich.com#p03)Pos.MammalianPlasmid
DNAgRNA only
[CRISPR11](https://www.sigmaaldrich.com/US/en/product/sigma/crispr11)CRISPR-Lenti Human EMX1 Positive Control, Cas9+gRNA Lenti Plasmid (vector)[LV01](https://www.sigmaaldrich.com#LV01)Pos.LentiviralPlasmid
DNACas9+gRNA
[CRISPR13](https://www.sigmaaldrich.com/US/en/product/sigma/crispr13)CRISPR-Lenti Human HPRT1 Positive Control, Cas9+gRNA Lenti Plasmid (vector)[LV01](https://www.sigmaaldrich.com#LV01)Pos.LentiviralPlasmid
DNACas9+gRNA
[CRISPR21](https://www.sigmaaldrich.com/US/en/product/sigma/crispr21)CRISPR-Lenti Human HPRT1 Positive Control, gRNA Only Lenti Plasmid (Sanger vector)[LV04](https://www.sigmaaldrich.com#LV04)Pos.LentiviralPlasmid
DNAgRNA only
[CRISPR11V](https://www.sigmaaldrich.com/US/en/product/sigma/crispr11v)CRISPR-Lenti Human EMX1 Positive Control, Cas9+gRNA Lentiviral Particles (vector)[LV01](https://www.sigmaaldrich.com#LV01)Pos.LentiviralLentivirusCas9+gRNA
[CRISPR13V](https://www.sigmaaldrich.com/US/en/product/sigma/crispr13v)CRISPR-Lenti Human HPRT1 Positive Control, Cas9+gRNA Lentiviral Particles (vector)[LV01](https://www.sigmaaldrich.com#LV01)Pos.LentiviralLentivirusCas9+gRNA
[CRISPR13H](https://www.sigmaaldrich.com/US/en/product/sigma/crispr13h)CRISPR-Lenti Human HPRT1 Positive Control, Cas9+gRNA Lentiviral Particles, High Titer (vector)[LV01](https://www.sigmaaldrich.com#LV01)Pos.LentiviralLentivirusCas9+gRNA
[CRISPR16H](https://www.sigmaaldrich.com/US/en/product/sigma/crispr16h)Gecko2-Lenti Human HPRT1 Positive Control, gRNA Only High Titer Lentiviral Particles (Gecko2 vector)[LV02](https://www.sigmaaldrich.com#LV02)Pos.LentiviralLentivirusgRNA only
[CRISPR21V](https://www.sigmaaldrich.com/US/en/product/sigma/crispr21v)CRISPR-Lenti Human HPRT1 Positive Control, gRNA Only Lentiviral Particles (Sanger vector)[LV04](https://www.sigmaaldrich.com#LV04)Pos.LentiviralLentivirusgRNA only
[CRISPRPL01](https://www.sigmaaldrich.com/US/en/product/sigma/crisprpl01)CRISPR GUS GAPDH Reporter Control for Monocots[p62](https://www.sigmaaldrich.com#p62)Pos.PlantPlasmid
DNACas9+gRNA
[CRISPRPL02](https://www.sigmaaldrich.com/US/en/product/sigma/crisprpl02)CRISPR GUS GAPDH Reporter Control for Dicots[p63](https://www.sigmaaldrich.com#p63)Pos.PlantPlasmid
DNACas9+gRNA
### Positive Controls
Product No.DescriptionVectorControl TypeSystemFormatgRNA Type
[CRISPR01](https://www.sigmaaldrich.com/US/en/product/sigma/crispr01)CRISPR Human EMX1 Positive Control Plasmid, gRNA Only Expression Plasmid, includes Cas9 Expression Plasmid (vector)[p03](https://www.sigmaaldrich.com#p03)Pos.MammalianPlasmid
DNAgRNA only
[CRISPR02](https://www.sigmaaldrich.com/US/en/product/sigma/crispr02)CRISPR Nickase Human EMX1 Positive
Control Plasmids (gRNA Only Expression
Plasmids, Includes Cas9-D10A Plasmid)[p03](https://www.sigmaaldrich.com#p03)Pos.MammalianPlasmid
DNAgRNA only
[CRISPR11](https://www.sigmaaldrich.com/US/en/product/sigma/crispr11)CRISPR-Lenti Human EMX1 Positive Control, Cas9+gRNA Lenti Plasmid (vector)[LV01](https://www.sigmaaldrich.com#LV01)Pos.LentiviralPlasmid
DNACas9+gRNA
[CRISPR13](https://www.sigmaaldrich.com/US/en/product/sigma/crispr13)CRISPR-Lenti Human HPRT1 Positive Control, Cas9+gRNA Lenti Plasmid (vector)[LV01](https://www.sigmaaldrich.com#LV01)Pos.LentiviralPlasmid
DNACas9+gRNA
[CRISPR21](https://www.sigmaaldrich.com/US/en/product/sigma/crispr21)CRISPR-Lenti Human HPRT1 Positive Control, gRNA Only Lenti Plasmid (Sanger vector)[LV04](https://www.sigmaaldrich.com#LV04)Pos.LentiviralPlasmid
DNAgRNA only
[CRISPR11V](https://www.sigmaaldrich.com/US/en/product/sigma/crispr11v)CRISPR-Lenti Human EMX1 Positive Control, Cas9+gRNA Lentiviral Particles (vector)[LV01](https://www.sigmaaldrich.com#LV01)Pos.LentiviralLentivirusCas9+gRNA
[CRISPR13V](https://www.sigmaaldrich.com/US/en/product/sigma/crispr13v)CRISPR-Lenti Human HPRT1 Positive Control, Cas9+gRNA Lentiviral Particles (vector)[LV01](https://www.sigmaaldrich.com#LV01)Pos.LentiviralLentivirusCas9+gRNA
[CRISPR13H](https://www.sigmaaldrich.com/US/en/product/sigma/crispr13h)CRISPR-Lenti Human HPRT1 Positive Control, Cas9+gRNA Lentiviral Particles, High Titer (vector)[LV01](https://www.sigmaaldrich.com#LV01)Pos.LentiviralLentivirusCas9+gRNA
[CRISPR16H](https://www.sigmaaldrich.com/US/en/product/sigma/crispr16h)Gecko2-Lenti Human HPRT1 Positive Control, gRNA Only High Titer Lentiviral Particles (Gecko2 vector)[LV02](https://www.sigmaaldrich.com#LV02)Pos.LentiviralLentivirusgRNA only
[CRISPR21V](https://www.sigmaaldrich.com/US/en/product/sigma/crispr21v)CRISPR-Lenti Human HPRT1 Positive Control, gRNA Only Lentiviral Particles (Sanger vector)[LV04](https://www.sigmaaldrich.com#LV04)Pos.LentiviralLentivirusgRNA only
[CRISPRPL01](https://www.sigmaaldrich.com/US/en/product/sigma/crisprpl01)CRISPR GUS GAPDH Reporter Control for Monocots[p62](https://www.sigmaaldrich.com#p62)Pos.PlantPlasmid
DNACas9+gRNA
[CRISPRPL02](https://www.sigmaaldrich.com/US/en/product/sigma/crisprpl02)CRISPR GUS GAPDH Reporter Control for Dicots[p63](https://www.sigmaaldrich.com#p63)Pos.PlantPlasmid
DNACas9+gRNA
## NON-MAMMALIAN CRISPR SYSTEMS
### [Plant CRISPR](https://www.sigmaaldrich.com/US/en/technical-documents/protocol/genomics/advanced-gene-editing/genome-editing-in-plants-with-crispr-cas9)
Explore ready-to-use cas9 and guide RNA (gRNA) expression plasmids for monocots and dicots
- Our plant CRISPR/Cas9 products are intended for *Agrobacterium*-mediated plant transformation or biolistic microparticle bombardment or protoplast transformation
- A codon optimized Cas9 protein and a gRNA are expressed from a single vector and provided as ready-to-use, transfection-grade DNA.
### [Bacterial CRISPR](https://www.sigmaaldrich.com/US/en/technical-documents/technical-article/genomics/advanced-gene-editing/crispr-cas-systems)
Our *E. coli* CRISPR/Cas-mediated Lambda-Red (λ-Red) homologous recombination (HR) vector system facilitates gene editing through the homology-directed repair (HDR) of double-stranded DNA breaks (DSBs) created by Cas9 endonuclease, using either ssDNA or dsDNA as an editing template.
## Vectors
### Mammalian System Vectors
Vector No.DescriptionOrder
ClonesCas9/gRNA
ContentFormat
AvailabilityOther Features
FluorophoreCas9 type
[p01](https://www.sigmaaldrich.com#p01)U6-gRNA:CMV-Cas9-2A-tGFP[p01 clones](https://www.sigmaaldrich.com/US/en/product/sigma/crispr)Cas9+gRNAPlasmid DNAGFP*S. pyogenes*
[p02](https://www.sigmaaldrich.com#p02)U6-gRNA:CMV-Cas9-2A-tRFP[p02 clones](https://www.sigmaaldrich.com/US/en/product/sigma/crispr)Cas9+gRNAPlasmid DNARFP*S. pyogenes*
[p04](https://www.sigmaaldrich.com#p04)U6-gRNA:CMV-eCas9-2a-tGFP[p04 clones](https://www.sigmaaldrich.com/US/en/product/sigma/crispr)Cas9+gRNAPlasmid DNAGFPenhanced specificity
[p03](https://www.sigmaaldrich.com#p03)U6-gRNA[p03 clones](https://www.sigmaaldrich.com/US/en/product/sigma/crispr)gRNA onlyPlasmid DNA
### Lentiviral Vectors
Vector
No.DescriptionOrder
ClonesCas9/gRNA ContentFormat
AvailabilitySelection
Method
[LV01](https://www.sigmaaldrich.com#LV01)U6-gRNA:ef1a-puro-2A-Cas9-2A-tGFP[LV01 clones](https://www.sigmaaldrich.com/US/en/product/sigma/crispr)Cas9+gRNAPlasmid DNA &
LentivirusFluorophore (GFP) & Puromycin
[LV05](https://www.sigmaaldrich.com#LV05)U6-gRNA:EF1a-Cas9+FLAG-2A-Puro[LV05 clones](https://www.sigmaaldrich.com/US/en/product/sigma/crispr)Cas9+gRNAPlasmid & LentivirusPuromycin
[LV06](https://www.sigmaaldrich.com#LV06)CRISPRa SAM U6-gRNA:EF1a-Puro[LV06 clones](https://www.sigmaaldrich.com/US/en/product/sigma/crispr)CRISPR activator gRNAPlasmid & LentivirusPuromycin
[LV07](https://www.sigmaaldrich.com#LV07)CRISPRa SAM U6-gRNA:EF1a-Zeo[LV07 clones](https://www.sigmaaldrich.com/US/en/product/sigma/crispr)CRISPR activator gRNAPlasmid & LentivirusZeo
[LV04](https://www.sigmaaldrich.com#LV04)U6-gRNA:hPGK-puro-2A-tBFP[Sanger QP KO clones](https://www.sigmaaldrich.com/US/en/product/sigma/crispr)
gRNA onlyPlasmid DNA &
LentivirusFluorophore (BFP) & Puromycin
[LV03](https://www.sigmaaldrich.com#LV03)U6-gRNA:ef1a-tGFP[LV03 clones](https://www.sigmaaldrich.com/US/en/product/sigma/crispr)gRNA onlyPlasmid DNA &
LentivirusFluorophore (GFP)
[LV02](https://www.sigmaaldrich.com#LV02)U6-gRNA:ef1a-puro[LV02 clones](https://www.sigmaaldrich.com/US/en/product/sigma/crispr)gRNA onlyPlasmid DNA &
LentivirusPuromycin
### Plant Vectors
Vector
No.DescriptionOrder
ClonesCas9/gRNA
ContentFormat
AvailabilitySpeciesPlasmid
TypeSelection
[p63](https://www.sigmaaldrich.com#p63)(dicot)U6-gRNA:CMV-Cas9-beta glucuronidase (Agrobacterium plasmid)[p63 clones](https://www.sigmaaldrich.com/US/en/product/sigma/crispr)Cas9+
gRNAPlasmid
DNADicotAgro-bacteriumBeta
Glucuron-
idase
[p55](https://www.sigmaaldrich.com#p55)(dicot)U6-gRNA:CMV-Cas9-bar (Agrobacterium plasmid)[p55 clones](https://www.sigmaaldrich.com/US/en/product/sigma/crispr)Cas9+
gRNAPlasmid
DNADicotAgro-bacteriumBiala-
phos
[p53](https://www.sigmaaldrich.com#p53)(dicot)U6-gRNA:CMV-Cas9-hyg (Agrobacterium plasmid)[p53 clones](https://www.sigmaaldrich.com/US/en/product/sigma/crispr)Cas9+
gRNAPlasmid
DNADicotAgro-bacteriumHygro-
mycin
[p54](https://www.sigmaaldrich.com#p54)(dicot)U6-gRNA:CMV-Cas9-neo (Agrobacterium plasmid)[p54 clones](https://www.sigmaaldrich.com/US/en/product/sigma/crispr)Cas9+
gRNAPlasmid
DNADicotAgro-bacteriumNeo-
mycin
[p61](https://www.sigmaaldrich.com#p61)(dicot)U6-gRNA:CMV-Cas9-bar (bioballistic plasmid)[p61 clones](https://www.sigmaaldrich.com/US/en/product/sigma/crispr)Cas9+
gRNAPlasmid
DNADicotBio-BallisticBiala-
phos
[p59](https://www.sigmaaldrich.com#p59)(dicot)U6-gRNA:CMV-Cas9-hyg (bioballistic plasmid)[p59 clones](https://www.sigmaaldrich.com/US/en/product/sigma/crispr)Cas9+
gRNAPlasmid
DNADicotBio-BallisticHygro-
mycin
[p60](https://www.sigmaaldrich.com#p60)(dicot)U6-gRNA:CMV-Cas9-neo (bioballistic plasmid)[p60 clones](https://www.sigmaaldrich.com/US/en/product/sigma/crispr)Cas9+
gRNAPlasmid
DNADicotBio-BallisticNeo-
mycin
[p62](https://www.sigmaaldrich.com#p62)(monocot)U6-gRNA:CMV-Cas9-beta glucuronidase (Agrobacterium plasmid)[p62 clones](https://www.sigmaaldrich.com/US/en/product/sigma/crispr)Cas9+
gRNAPlasmid
DNAMonocotAgro-bacteriumBeta
Glucuron-
idase
[p52](https://www.sigmaaldrich.com#p52)(monocot)U6-gRNA:CMV-Cas9-bar (Agrobacterium plasmid)[p52 clones](https://www.sigmaaldrich.com/US/en/product/sigma/crispr)Cas9+
gRNAPlasmid
DNAMonocotAgro-bacteriumBiala-
phos
[p50](https://www.sigmaaldrich.com#p50)(monocot) U6-gRNA:CMV-Cas9-hyg (Agrobacterium plasmid)[p50 clones](https://www.sigmaaldrich.com/US/en/product/sigma/crispr)Cas9+
gRNAPlasmid
DNAMonocotAgro-bacteriumHygro-
mycin
[p51](https://www.sigmaaldrich.com#p51)(monocot)U6-gRNA:CMV-Cas9-neo (Agrobacterium plasmid)[p51 clones](https://www.sigmaaldrich.com/US/en/product/sigma/crispr)Cas9+
gRNAPlasmid
DNAMonocotAgro-bacteriumNeo-
mycin
[p58](https://www.sigmaaldrich.com#p58)(monocot)U6-gRNA:CMV-Cas9-bar (bioballistic plasmid)[p58 clones](https://www.sigmaaldrich.com/US/en/product/sigma/crispr)Cas9+
gRNAPlasmid
DNAMonocotBio-BallisticBiala-
phos
[p56](https://www.sigmaaldrich.com#p56)(monocot)U6-gRNA:CMV-Cas9-hyg (bioballistic plasmid)[p56 clones](https://www.sigmaaldrich.com/US/en/product/sigma/crispr)Cas9+
gRNAPlasmid
DNAMonocotBio-BallisticHygro-
mycin
[p57](https://www.sigmaaldrich.com#p57)(monocot)U6-gRNA:CMV-Cas9-neo (bioballistic plasmid)[p57 clones](https://www.sigmaaldrich.com/US/en/product/sigma/crispr)Cas9+
gRNAPlasmid
DNAMonocotBio-BallisticNeo-
mycin
### Bacterial Vectors
Vector No.DescriptionOrder ClonesCas9/gRNA ContentFormat AvailabilitySelection
[p10](https://www.sigmaaldrich.com#p10)(bacteria) J23119-gRNA-amp
[p10 clones](https://www.sigmaaldrich.com/US/en/product/sigma/crispr)
gRNA only
Plasmid DNAAmpicillin
### Vector Maps and Sequences
*Click on the images below for a larger view.*
[](https://www.sigmaaldrich.com)__p01 Vector__
[](https://www.sigmaaldrich.com/content/dam/cms-commons/sigmaaldrich/marketing/global/images/technical-documents/articles/genomics/advanced-gene-editing/vector-p01.jpg)
[Download Fully Annotated p01 Map](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/fasta-files/p01.gb)
[Download p01 FASTA Sequence](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/fasta-files/p01.fasta)
[](https://www.sigmaaldrich.com)__p02 Vector__
[](https://www.sigmaaldrich.com/content/dam/cms-commons/sigmaaldrich/marketing/global/images/technical-documents/articles/genomics/advanced-gene-editing/vector-p02.jpg)
[Download Fully Annotated p02 Map](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/fasta-files/p02.gb)
[Download p02 FASTA Sequence](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/fasta-files/p02.fasta)
[](https://www.sigmaaldrich.com)__p03 Vector__
[](https://www.sigmaaldrich.com/content/dam/cms-commons/sigmaaldrich/marketing/global/images/technical-documents/articles/genomics/advanced-gene-editing/vector-p03.jpg)
[Download Fully Annotated p03 Map](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/fasta-files/p03.gb)
[Download p03 FASTA Sequence](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/fasta-files/p03.fasta)
[](https://www.sigmaaldrich.com)__p04 Vector__
[](https://www.sigmaaldrich.com/content/dam/cms-commons/sigmaaldrich/marketing/global/images/technical-documents/articles/genomics/advanced-gene-editing/vector-p04.jpg)
[Download Fully Annotated p04 Map](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/fasta-files/p04.gb)
[Download p04 FASTA Sequence](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/fasta-files/p04.fasta)
[](https://www.sigmaaldrich.com)__p05 Vector__
[](https://www.sigmaaldrich.com/content/dam/cms-commons/sigmaaldrich/marketing/global/images/technical-documents/articles/genomics/advanced-gene-editing/vector-p05.jpg)
[Download Fully Annotated p05 Map](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/fasta-files/p05.gb)
[Download p05 FASTA Sequence](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/fasta-files/p05.fasta)
[](https://www.sigmaaldrich.com)__p10 Vector__
[](https://www.sigmaaldrich.com/content/dam/cms-commons/sigmaaldrich/marketing/global/images/technical-documents/articles/genomics/advanced-gene-editing/vector-p10.jpg)
[Download Fully Annotated p10 Map](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/fasta-files/p10.gb)
[Download p10 FASTA Sequence](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/fasta-files/p10.fasta)
[](https://www.sigmaaldrich.com)__p50 Vector__
[](https://www.sigmaaldrich.com/content/dam/cms-commons/sigmaaldrich/marketing/global/images/technical-documents/articles/genomics/advanced-gene-editing/vector-p50.jpg)
[Download Fully Annotated p50 Map](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/fasta-files/p50.gb)
[Download p50 FASTA Sequence](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/fasta-files/p50.fasta)
[](https://www.sigmaaldrich.com)__p51 Vector__
[](https://www.sigmaaldrich.com/content/dam/cms-commons/sigmaaldrich/marketing/global/images/technical-documents/articles/genomics/advanced-gene-editing/vector-p51.jpg)
[Download Fully Annotated p51 Map](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/fasta-files/p51.gb)
[Download p51 FASTA Sequence](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/fasta-files/p51.fasta)
[](https://www.sigmaaldrich.com)__p52 Vector__
[](https://www.sigmaaldrich.com/content/dam/cms-commons/sigmaaldrich/marketing/global/images/technical-documents/articles/genomics/advanced-gene-editing/vector-p52.jpg)
[Download Fully Annotated p52 Map](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/fasta-files/p52.gb)
[Download p52 FASTA Sequence](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/fasta-files/p52.fasta)
[](https://www.sigmaaldrich.com)__p53 Vector__
[](https://www.sigmaaldrich.com/content/dam/cms-commons/sigmaaldrich/marketing/global/images/technical-documents/articles/genomics/advanced-gene-editing/vector-p53.jpg)
[Download Fully Annotated p53 Map](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/fasta-files/p53.gb)
[Download p53 FASTA Sequence](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/fasta-files/p53.fasta)
[](https://www.sigmaaldrich.com)__p54 Vector__
[](https://www.sigmaaldrich.com/content/dam/cms-commons/sigmaaldrich/marketing/global/images/technical-documents/articles/genomics/advanced-gene-editing/vector-p54.jpg)
[Download Fully Annotated p54 Map](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/fasta-files/p54.gb)
[Download p54 FASTA Sequence](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/fasta-files/p54.fasta)
[](https://www.sigmaaldrich.com)__p55 Vector__
[](https://www.sigmaaldrich.com/content/dam/cms-commons/sigmaaldrich/marketing/global/images/technical-documents/articles/genomics/advanced-gene-editing/vector-p55.jpg)
[Download Fully Annotated p55 Map](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/fasta-files/p55.gb)
[Download p5 FASTA Sequence](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/fasta-files/p55.fasta)
[](https://www.sigmaaldrich.com)__p56 Vector__
[](https://www.sigmaaldrich.com/content/dam/cms-commons/sigmaaldrich/marketing/global/images/technical-documents/articles/genomics/advanced-gene-editing/vector-p56.jpg)
[Download Fully Annotated p56 Map](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/fasta-files/p01.gb)
[Download p56 FASTA Sequence](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/fasta-files/p56.fasta)
[](https://www.sigmaaldrich.com)__p57 Vector__
[](https://www.sigmaaldrich.com/content/dam/cms-commons/sigmaaldrich/marketing/global/images/technical-documents/articles/genomics/advanced-gene-editing/vector-p57.jpg)
[Download Fully Annotated p57 Map](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/fasta-files/p57.gb)
[Download p57 FASTA Sequence](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/fasta-files/p57.fasta)
[](https://www.sigmaaldrich.com)__p58 Vector__
[](https://www.sigmaaldrich.com/content/dam/cms-commons/sigmaaldrich/marketing/global/images/technical-documents/articles/genomics/advanced-gene-editing/vector-p58.jpg)
[Download Fully Annotated p58 Map](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/fasta-files/p01.gb)
[Download p58 FASTA Sequence](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/fasta-files/p58.fasta)
[](https://www.sigmaaldrich.com)__p59 Vector__
[](https://www.sigmaaldrich.com/content/dam/cms-commons/sigmaaldrich/marketing/global/images/technical-documents/articles/genomics/advanced-gene-editing/vector-p59.jpg)
[Download Fully Annotated p59 Map](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/fasta-files/p59.gb)
[Download p59 FASTA Sequence](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/fasta-files/p59.fasta)
[](https://www.sigmaaldrich.com)__p60 Vector__
[](https://www.sigmaaldrich.com/content/dam/cms-commons/sigmaaldrich/marketing/global/images/technical-documents/articles/genomics/advanced-gene-editing/vector-p60.jpg)
[Download Fully Annotated p60 Map](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/fasta-files/p60.gb)
[Download p60 FASTA Sequence](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/fasta-files/p60.fasta)
[](https://www.sigmaaldrich.com)__p61 Vector__
[](https://www.sigmaaldrich.com/content/dam/cms-commons/sigmaaldrich/marketing/global/images/technical-documents/articles/genomics/advanced-gene-editing/vector-p61.jpg)
[Download Fully Annotated p61 Map](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/fasta-files/p61.gb)
[Download p61 FASTA Sequence](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/fasta-files/p61.fasta)
[](https://www.sigmaaldrich.com)__p62 Vector__
[](https://www.sigmaaldrich.com/content/dam/cms-commons/sigmaaldrich/marketing/global/images/technical-documents/articles/genomics/advanced-gene-editing/vector-p62.jpg)
[Download Fully Annotated p62 Map](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/fasta-files/p62.gb)
[Download p62 FASTA Sequence](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/fasta-files/p62.fasta)
[](https://www.sigmaaldrich.com)__p63 Vector__
[](https://www.sigmaaldrich.com/content/dam/cms-commons/sigmaaldrich/marketing/global/images/technical-documents/articles/genomics/advanced-gene-editing/vector-p63.jpg)
[Download Fully Annotated p63 Map](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/fasta-files/p63.gb)
[Download p01 FASTA Sequence](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/fasta-files/p63.fasta)
[](https://www.sigmaaldrich.com)__LV01 Vector__
[](https://www.sigmaaldrich.com/content/dam/cms-commons/sigmaaldrich/marketing/global/images/technical-documents/articles/genomics/advanced-gene-editing/vector-lv01.jpg)
[Download Fully Annotated LV01 Map](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/fasta-files/lv01.gb)
[Download LV01 FASTA Sequence](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/fasta-files/lv01.fasta)
[](https://www.sigmaaldrich.com)__LV02 Vector__
[](https://www.sigmaaldrich.com/content/dam/cms-commons/sigmaaldrich/marketing/global/images/technical-documents/articles/genomics/advanced-gene-editing/vector-lv02.jpg)
[Download Fully Annotated LV02 Map](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/fasta-files/lv02.gb)
[Download LV02 FASTA Sequence](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/fasta-files/lv02.fasta)
[](https://www.sigmaaldrich.com)__LV03 Vector__
[](https://www.sigmaaldrich.com/content/dam/cms-commons/sigmaaldrich/marketing/global/images/technical-documents/articles/genomics/advanced-gene-editing/vector-lv03.jpg)
[Download Fully Annotated LV03 Map](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/fasta-files/lv03.gb)
[Download LV03 FASTA Sequence](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/fasta-files/lv03.fasta)
[](https://www.sigmaaldrich.com)__LV04 Vector__
[](https://www.sigmaaldrich.com/content/dam/cms-commons/sigmaaldrich/marketing/global/images/technical-documents/articles/genomics/advanced-gene-editing/vector-lv04.jpg)
[Download Fully Annotated LV04 Map](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/fasta-files/lv04.gb)
[Download LV04 FASTA Sequence](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/fasta-files/lv04.fasta)
[](https://www.sigmaaldrich.com)__LV05 Vector__
[](https://www.sigmaaldrich.com/content/dam/cms-commons/sigmaaldrich/marketing/global/images/technical-documents/articles/genomics/advanced-gene-editing/vector-lv05.jpg)
[Download Fully Annotated LV05 Map](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/fasta-files/lv05.gb)
[Download LV05 FASTA Sequence](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/319/136/lv05-vector.zip)
[](https://www.sigmaaldrich.com)__LV06 Vector__
[](https://www.sigmaaldrich.com/content/dam/cms-commons/sigmaaldrich/marketing/global/images/technical-documents/articles/genomics/advanced-gene-editing/vector-lv06n.jpg)
[Download Fully Annotated LV06 Map](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/fasta-files/lv06.gb)
[Download LV06 FASTA Sequence](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/fasta-files/lv06.fasta)
[](https://www.sigmaaldrich.com)__LV07 Vector__
[](https://www.sigmaaldrich.com/content/dam/cms-commons/sigmaaldrich/marketing/global/images/technical-documents/articles/genomics/advanced-gene-editing/vector-lv07n.jpg)
[Download Fully Annotated LV07 Map](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/fasta-files/lv07.gb)
[Download LV07 FASTA Sequence](https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/fasta-files/lv07.fasta)
[](https://www.sigmaaldrich.com)__LV17 Vector__
[](https://www.sigmaaldrich.com/content/dam/cms-commons/sigmaaldrich/marketing/global/images/technical-documents/articles/genomics/advanced-gene-editing/vector-lv17.jpg)
[](https://www.sigmaaldrich.com)__KRAB-dCas9 Vector__
[](https://www.sigmaaldrich.com/content/dam/cms-commons/sigmaaldrich/marketing/global/images/technical-documents/articles/genomics/advanced-gene-editing/vector-krab-dcas9.jpg)
If you need any further information, please [contact us here](https://www.sigmaaldrich.com/US/en/services/crispr-request-form) and a customer service representative will get back to you.
### [](https://www.sigmaaldrich.com)Product Guarantee
We are so confident in the performance of our synthetic gRNA products, that we fully guarantee the quality and performance of any gRNA we produce, including custom sequences. If your crRNA or sgRNA do not yield detectable cleavage at the intended target site, we will provide you a one-time replacement, free of charge.
To qualify for this guarantee, please send an image or sequencing data from a single experiment demonstrating detectable cleavage using one of our positive controls, side-by-side with the negative results from your synthetic gRNA. To receive your replacement, simply email [oligotechserv@milliporesigma.com](mailto:oligotechserv@milliporesigma.com) and include sample data from a representative experiment (T7E1, TIDE, or NGS).
__Related Articles__
- [Ribonucleoprotein (RNP) Protocols Using Synthetic sgRNAs and Cas9 proteins](https://www.sigmaaldrich.com/US/en/technical-documents/protocol/genomics/advanced-gene-editing/ribonucleoprotein-based-geneome-editing)
- [PURedit™ CRISPR Synthetic Guide RNAs and Cas9 Protein](https://www.sigmaaldrich.com/US/en/technical-documents/protocol/genomics/advanced-gene-editing/cas9-ribonucleoprotein-complexes)
- [Reverse Transfection of Plasmid DNA](https://www.sigmaaldrich.com/US/en/technical-documents/technical-article/cell-culture-and-cell-culture-analysis/transfection-and-gene-editing/xtg-reverse-transfect-pdna)
- [Cas9-Mediated Recombineering for Kdo2-Lipid A](https://www.sigmaaldrich.com/US/en/technical-documents/technical-article/genomics/advanced-gene-editing/crispr-cas-systems)
- [Long Oligos](https://www.sigmaaldrich.com/US/en/technical-documents/technical-article/genomics/advanced-gene-editing/custom-dna-long-oligos)
- [Validating CRISPR/Cas9-mediated Gene Editing](https://www.sigmaaldrich.com/US/en/technical-documents/technical-article/genomics/advanced-gene-editing/validating-crispr-cas9-mediated-gene-editing)
- [Methods for Enhancing Lentiviral Transduction Efficiency](https://www.sigmaaldrich.com/US/en/technical-documents/technical-article/genomics/advanced-gene-editing/enhancing-lentiviral)
- [PURedit® cytosine base editor Proteins](https://www.sigmaaldrich.com/US/en/technical-documents/technical-article/genomics/advanced-gene-editing/puredit-cytosine-base-editor-proteins)
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