Read the latest developments in CRISPR research

The most recent papers in CRISPR research

Adriana CarolinaGonzalez1MichaelaSchweizer2SebastianJagdmann1ChristianBernreuther3ThomasReinheckel4PaulSaftig1MarkusDamme15
Variants in the phospholipase D3 (PLD3) gene have genetically been linked to late-onset Alzheimer's disease. We present a detailed biochemical analysis of PLD3 and reveal its endogenous localization in endosomes and lysosomes. PLD3 reaches lysosomes as a type II transmembrane protein via a (for mammalian cells) uncommon intracelRead More
Sylwia Gawrzak, Lorenzo Rinaldi, […]Roger R. Gomis
For many patients with breast cancer, symptomatic bone metastases appear after years of latency. How micrometastatic lesions remain dormant and undetectable before initiating colonization is unclear. Here, we describe a mechanism involved in bone metastatic latency of oestrogen receptor-positive (ER+) breast cancer. Using an in Read More
Vivek Iyer , Katharina Boroviak , Mark Thomas, Brendan Doe, Laura Riva, Edward Ryder , David J. Adams
July 9, 2018
CRISPR-Cas9 technologies have transformed genome-editing of experimental organisms and have immense therapeutic potential. Despite significant advances in our understanding of the CRISPR-Cas9 system, concerns remain over the potential for off-target effects. Recent studies have addressed these concerns using whole-genome sequencRead More
Araci M.R.RondonafVitor AlmeidaaTaináGomesaBrunno R.F.VerçozabcRenato S.CarvalhodSandraKönigeJuliany C.F.RodriguesbcClaudia dos S.MermelsteineHenri H.VersteegfRobson Q.Monteiro
Extracellular vesicles, such as microvesicles (MVs), were identified as important players in tumor progression and acquisition of an aggressive phenotype. Tissue factor (TF) is a transmembrane protein that initiates the blood coagulation cascade. In tumor cells, TF has been associated with aggressiveness and cancer progression. Read More
Kara L.Perks1GiuliaRossetti1IrinaKuznetsova1Laetitia A.Hughes1Judith A.Ermer1NicolaFerreira1Jakob D.Busch2Danielle L.Rudler1HenrikSpahr2ThomasSchöndorf2Ann-Marie J.Shearwood1Helena M.Viola3Stefan J.Siira1Livia C.Hool34DusankaMilenkovic2Nils-GöranLarsson25OliverRackham16AleksandraFilipovska167
The regulation of mitochondrial RNA life cycles and their roles in ribosome biogenesis and energy metabolism are not fully understood. We used CRISPR/Cas9 to generate heart- and skeletal-muscle-specific knockout mice of the pentatricopeptide repeat domain protein 1, PTCD1, and show that its loss leads to severe cardiomyopathy anRead More
The recently emerged CRISPR/Cas9 technology is the most flexible means to produce targeted mutations at the genomic loci in a variety of organisms. In Crustaceans, molt-inhibiting hormone (MIH) is an important negative-regulatory factor and plays a key role in suppressing the molting process. However, whether precise disruption Read More
The global adoption of vaccines to combat disease is hampered by the high cost of vaccine manufacturing. The work described herein follows two previous publications (van der Sanden et al., 2016; Wu et al., 2017) that report a strategy to enhance poliovirus and rotavirus vaccine production through genetic modification of the VeroRead More
Min6 cells, a mouse β cell line derived from transgenic mouse expressing the large T-antigen of SV40 in pancreatic beta cells, are commonly utilized as an in vitro cellular model for investigating targets involved in insulin secretion. Epac2, an exchange protein that can be directly activated by cyclic AMP (cAMP), is critical foRead More
Chen Fuqiang, Ding Xiao, Feng Yongmei, Seebeck Timothy, Jiang Yanfang, Davis GregoryD
Nature Communications 2017-04-01
Bacterial CRISPR-Cas systems comprise diverse effector endonucleases with different targeting ranges, specificities and enzymatic properties, but many of them are inactive in mammalian cells and are thus precluded from genome-editing applications. Here we show that the type II-B FnCas9 from Francisella novicida possesses novel p...Read More
Benedetti Lorena, Cereda Matteo, Monteverde LeeAnn, Desai Nikita, Ciccarelli FrancescaD
Oncotarget 2017-04-01
Cohesin is a multi-protein complex that tethers sister chromatids during mitosis and mediates DNA repair, genome compartmentalisation and regulation of gene expression. Cohesin subunits frequently acquire cancer loss-of-function alterations and act as tumour suppressors in several tumour types. This has led to increased interest...Read More
Metzakopian Emmanouil, Strong Alex, Iyer Vivek, Hodgkins Alex, Tzelepis Konstantinos, Antunes Liliana, Friedrich MathiasJ, Kang Qiaohua, Davidson Teresa, Lamberth Jacob, Hoffmann Christina, Davis GregoryD, Vassiliou GeorgeS, Skarnes WilliamC, Bradley Allan
Scientific reports 2017-05-01
CRISPR-Cas9 technology has accelerated biological research becoming routine for many laboratories. It is rapidly replacing conventional gene editing techniques and has high utility for both genome-wide and gene-focussed applications. Here we present the first individually cloned CRISPR-Cas9 genome wide arrayed sgRNA libraries co...Read More
Aydin Seyid Personne Yoann Newire Enas Laverick Rebecca Russell Oliver Roberts AdamP Enne VirveI
Journal of Antimicrobial Chemotherapy 2017-05-01
Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) and their associated cas genes are sequence-specific DNA nuclease systems found in bacteria and archaea. CRISPR/Cas systems use RNA transcripts of previously acquired DNA (spacers) to target invading genetic elements with the same sequence, including plasmids. In...Read More
Bautista MariaA Black JesseA Youngblut NicholasD Whitaker RachelJ
Viruses 2017-05-01
In the past decade, molecular surveys of viral diversity have revealed that viruses are the most diverse and abundant biological entities on Earth. In culture, however, most viral isolates that infect microbes are represented by a few variants isolated on type strains, limiting our ability to study how natural variation affects ...Read More
Rosenbluh Joseph Xu Han Harrington William Gill Stanley Wang Xiaoxing Vazquez Francisca Root DavidE Tsherniak Aviad Hahn WilliamC
Nature Communications 2017-05-01
CRISPR-Cas9 provides the means to perform genome editing and facilitates loss-of-function screens. However, we and others demonstrated that expression of the Cas9 endonuclease induces a gene-independent response that correlates with the number of target sequences in the genome. An alternative approach to suppressing gene express...Read More
Benahmed-Miniuk Fairouz Kresz Mat Kanaujiya JitendraK Southgate ChristopherD
Pharmaceutical patent analyst 2017-05-01
Unlike with zinc finger nuclease and transcriptional activator-like effector nuclease DNA modification technologies that rely on lead proteins, developed through expensive and time-consuming processes, the clustered regularly interspaced short palindromic repeat (CRISPR)/Cas system has rapidly emerged as the most promising gene-...Read More
Men Ke Duan Xingmei He Zhiyao Yang Yang Yao Shaohua Wei Yuquan
SCIENCE CHINA Life Science 2017-05-01
The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) protein 9 system (CRISPR/Cas9) provides a powerful tool for targeted genetic editing. Directed by programmable sequence-specific RNAs, this system introduces cleavage and double-stranded breaks at target sites precisely. Compared to pr...Read More
Zhang Xiaohui Wang Liren Liu Mingyao Li Dali
SCIENCE CHINA Life Science 2017-05-01
CRISPR/Cas9 is a versatile genome-editing tool which is widely used for modifying the genome of both prokaryotic and eukaryotic organisms for basic research and applications. An increasing number of reports have demonstrated that CRISPR/Cas9-mediated genome editing is a powerful technology for gene therapy. Here, we review the r...Read More
Lupton Charlotte Sengupta Mohini Cheng Ruey-Kuang Chia Joanne Thirumalai Vatsala Jesuthasan Suresh
eNeuro 2017-05-01
Learning how to actively avoid a predictable threat involves two steps: recognizing the cue that predicts upcoming punishment and learning a behavioral response that will lead to avoidance. In zebrafish, ventral habenula (vHb) neurons have been proposed to participate in both steps by encoding the expected aversiveness of a stim...Read More
Metzakopian Emmanouil Strong Alex Iyer Vivek Hodgkins Alex Tzelepis Konstantinos Antunes Liliana Friedrich MathiasJ Kang Qiaohua Davidson Teresa Lamberth Jacob Hoffmann Christina Davis GregoryD Vassiliou GeorgeS Skarnes WilliamC Bradley Allan
Scientific reports 2017-05-01
CRISPR-Cas9 technology has accelerated biological research becoming routine for many laboratories. It is rapidly replacing conventional gene editing techniques and has high utility for both genome-wide and gene-focussed applications. Here we present the first individually cloned CRISPR-Cas9 genome wide arrayed sgRNA libraries co...Read More
Dorr CaseyR Remmel RoryP Muthusamy Amutha Fisher James Moriarity Branden Yasuda Kazuto Wu Baolin Guan Weihua Schuetz ErinG Oetting WilliamS Jacobson PamalaA Israni AjayK
Drug Metabolism and Disposition 2017-05-01
CRISPR/Cas9 engineering of the CYP3A5 *3 locus (rs776746) in human liver cell line HuH-7 (CYP3A5 *3/*3) led to three CYP3A5 *1 cell lines by deletion of the exon 3B splice junction or point mutation. Cell lines CYP3A5 *1/*3 sd (single deletion), CYP3A5 *1/*1 dd (double deletion) or CYP3A5 *1/*3 pm (point mutation) expressed the ...Read More
Angelos MathewG Ruh PaigeN Webber BeauR Blum RobertH Ryan CaitlinD Bendzick Laura Shim Seonhui Yingst AshleyM Tufa DejeneM Verneris MichaelR Kaufman DanS
Blood 2017-05-01
The aryl hydrocarbon receptor (AHR) plays an important physiological role in hematopoiesis. AHR is highly expressed in hematopoietic stem/progenitor cells (HSPCs) and inhibition of AHR results in a marked expansion of human umbilical cord blood-derived HSPCs following cytokine stimulation. It is unknown whether AHR also contribu...Read More
Sarkari Parveen Marx Hans Blumhoff MarzenaL Mattanovich Diethard Sauer Michael Steiger MatthiasG
Bioresource Technology 2017-05-01
Metabolic engineering requires functional genetic tools for easy and quick generation of multiple pathway variants. A genetic engineering toolbox for A. niger is presented, which facilitates the generation of strains carrying heterologous expression cassettes at a defined genetic locus. The system is compatible with Golden Gate ...Read More
Wei Lai Chiu DavidKung-Chun Tsang FeliceHo-Ching Law DickyCheuk-Ting Cheng CarolLai-Hung Au SandyLeung-Kuen Lee JoyceMan-Fong Wong CarmenChak-Lui Ng IreneOi-Lin Wong Chun-Ming
Journal of Hepatology 2017-05-01
Hepatocellular carcinoma (HCC) is a major leading cause of cancer mortality worldwide. Epigenetic deregulation is a common trait of human HCC. G9s is an important epigenetics regulator. However, its roles in liver carcinogenesis remain to be investigated.Read More
Zaidi SyedShan-E-Ali Mahfouz MagdyM Mansoor Shahid
Trends in Plant Science 2017-05-01
Clustered regularly interspaced palindromic repeats (CRISPR)-CRISPR-associated proteins (CRISPR-Cas), a groundbreaking genome-engineering tool, has facilitated targeted trait improvement in plants. Recently, CRISPR-CRISPR from Prevotella and Francisella 1 (Cpf1) has emerged as a new tool for efficient genome editing, including D...Read More
Wu Jun Wang Pengcheng Li Lin Williams NicoleL Ji Debin Zahurancik WalterJ You Changjun Wang Jianshuang Suo Zucai Wang Yinsheng
Nucleic Acids Research 2017-05-01
Metabolic activation of some N-nitroso compounds (NOCs), an important class of DNA damaging agents, can induce the carboxymethylation of nucleobases in DNA. Very little was previously known about how the carboxymethylated DNA lesions perturb DNA replication in human cells. Here, we investigated the effects of five carboxymethyla...Read More
Kalatzis PanosG Rrbo NannaIben Castillo Daniel Mauritzen JesperJuel Jrgensen Jhanna Kokkari Constantina Zhang Faxing Katharios Pantelis Middelboe Mathias
Viruses 2017-05-01
Nineteen Vibrio anguillarum-specific temperate bacteriophages isolated across Europe and Chile from aquaculture and environmental sites were genome sequenced and analyzed for host range, morphology and life cycle characteristics. The phages were classified as Siphoviridae with genome sizes between 46,006 and 54,201 bp. All 19 ph...Read More
Liu Xiaoqin Yagi Hisato Saeed Shazina Bais AbhaS Gabriel GeorgeC Chen Zhaohan Peterson KevinA Li You Schwartz MollyC Reynolds WilliamT Saydmohammed Manush Gibbs Brian Wu Yijen Devine William Chatterjee Bishwanath Klena NikolaiT Kostka Dennis deMesyBentley KarenL Ganapathiraju MadhaviK Dexheimer Phillip Leatherbury Linda Khalifa Omar Bhagat Anchit Zahid Maliha Pu William Watkins Simon Grossfeld Paul Murray StephenA Porter GeorgeA Tsang Michael Martin LisaJ WoodrowBenson D Aronow BruceJ Lo CeciliaW
Nature Genetics 2017-05-01
Congenital heart disease (CHD) affects up to 1% of live births. Although a genetic etiology is indicated by an increased recurrence risk, sporadic occurrence suggests that CHD genetics is complex. Here, we show that hypoplastic left heart syndrome (HLHS), a severe CHD, is multigenic and genetically heterogeneous. Using mouse for...Read More
Rogers ZoN McFarland ChristopherD Winters IanP Naranjo Santiago Chuang Chen-Hua Petrov Dmitri Winslow MonteM
Nature Methods 2017-05-01
Cancer growth is a multistage, stochastic evolutionary process. While cancer genome sequencing has been instrumental in identifying the genomic alterations that occur in human tumors, the consequences of these alterations on tumor growth remain largely unexplored. Conventional genetically engineered mouse models enable the study...Read More
Petris Gianluca Casini Antonio Montagna Claudia Lorenzin Francesca Prandi Davide Romanel Alessandro Zasso Jacopo Conti Luciano Demichelis Francesca Cereseto Anna
Nature Communications 2017-05-01
In vivo application of the CRISPR-Cas9 technology is still limited by unwanted Cas9 genomic cleavages. Long-term expression of Cas9 increases the number of genomic loci non-specifically cleaved by the nuclease. Here we develop a Self-Limiting Cas9 circuit for Enhanced Safety and specificity (SLiCES) which consists of an expressi...Read More
Almarza D Cucchiarini M Loughlin J
Osteoarthritis and Cartilage 2017-05-01
Xie Qingqing Lin Qi Li Dezhi Chen Jianming
Biochemical and Biophysical Research Communications 2017-05-01
Gastrointestinal stromal tumors (GISTs) are the most common mesenchymal neoplasms originating from the gastrointestinal tract with gain of function mutations in receptor tyrosine kinases KIT or platelet-derived growth factor receptor A (PDGFRA). The main effective treatment for GISTs is tyrosine kinase inhibitors, such as imatin...Read More
Chang Hetan Liu Yang Ai Dong Jiang Xingchuan Dong Shuanglin Wang Guirong
Current Biology 2017-05-01
Many insect species use multi-component sex pheromones to discriminate among potential mating partners [1-5]. In moths, pheromone blends tend to be dominated by one or two major components, but behavioral responses are frequently optimized by the inclusion of less abundant minor components [6]. An increasing number of studies ha...Read More
Yao Xuan Wang Xing Liu Junlai Hu Xinde Shi Linyu Shen Xiaowen Ying Wenqin Sun Xinyao Wang Xin Huang Pengyu Yang Hui
EBioMedicine 2017-05-01
Precisely targeted genome editing is highly desired for clinical applications. However, the widely used homology-directed repair (HDR)-based genome editing strategies remain inefficient for certain in vivo applications. We here demonstrate a microhomology-mediated end-joining (MMEJ)-based strategy for precisely targeted gene int...Read More
Xu Lei Yang Huan Gao Yang Chen Zeyu Xie Liangfu Liu Yulin Liu Ying Wang Xiaobao Li Hanwei Lai Weifeng He Yuan Yao Anzhi Ma Liying Shao Yiming Zhang Bin Wang Chengyan Chen Hu Deng Hongkui
Molecular Therapy 2017-05-01
Transplantation of hematopoietic stem cells (HSCs) with a naturally occurring CCR5 mutation confers a loss of detectable HIV-1 in the patient, making ablation of the CCR5 gene in HSCs an ideal therapy for an HIV-1 cure. Although CCR5 disruption has been attempted in CD4(+) Tcells and hematopoietic stem/progenitor cells (HSPCs), ...Read More
Jiang Dongneng Chen Jinlin Fan Zheng Tan Dejie Zhao Jiue Shi Hongjuan Liu Zhilong Tao Wenjing Li Minghui Wang Deshou
Developmental Biology 2017-05-01
Wilms tumor 1 (Wt1) is an essential factor for urogenital system development. Teleosts have two wt1s, named as wt1a and wt1b. In this study, the expression pattern of wt1a and wt1b and their functions on the urogenital system were analyzed by in situ hybridization and CRISPR/Cas9. wt1a was found to be expressed in the glomerulus...Read More
deGalarreta MarinaRuiz Lujambio Amaia
Journal of Hepatology 2017-05-01
The recent development of gene editing platforms enables making precise changes in the genome of eukaryotic cells. Programmable nucleases, such as meganucleases, zinc finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs), and clustered regularly interspaced short palindromic repeat (CRISPR)-associated...Read More
He Zhi-Yao Men Ke Qin Zhou Yang Yang Xu Ting Wei Yu-Quan
SCIENCE CHINA Life Science 2017-05-01
The clustered regularly interspaced short palindromic repeats (CRISPR)-associated protein 9 (CRISPR-Cas9) system provides a novel genome editing technology that can precisely target a genomic site to disrupt or repair a specific gene. Some CRISPR-Cas9 systems from different bacteria or artificial variants have been discovered or...Read More
Ren Xingjie Holsteens Kristof Li Haiyi Sun Jin Zhang Yifan Liu Lu-Ping Liu Qingfei Ni Jian-Quan
SCIENCE CHINA Life Science 2017-05-01
Nowadays, genome editing tools are indispensable for studying gene function in order to increase our knowledge of biochemical processes and disease mechanisms. The extensive availability of mutagenesis and transgenesis tools make Drosophila melanogaster an excellent model organism for geneticists. Early mutagenesis tools relied ...Read More
Wang Yanling Meng Zhigang Liang Chengzhen Meng Zhaohong Wang Yuan Sun Guoqing Zhu Tao Cai Yongping Guo Sandui Zhang Rui Lin Yi
SCIENCE CHINA Life Science 2017-05-01
Ran Yidong Liang Zhen Gao Caixia
SCIENCE CHINA Life Science 2017-05-01
Many genome editing tools have been developed and new ones are anticipated; some have been extensively applied in plant genetics, biotechnology and breeding, especially the CRISPR/Cas9 system. These technologies have opened up a new era for crop improvement due to their precise editing of user-specified sequences related to agro...Read More
Wu Xuebing Bartel DavidP
Cell 2017-05-01
The physiological relevance of structures within mammalian mRNAs has been elusive, as these mRNAs are less folded in cells than invitro and have predicted secondary structures no more stable than those of random sequences. Here, we investigate the possibility that mRNA structures facilitate the 3'-end processing of thousands of ...Read More
Hirosawa Moe Fujita Yoshihiko Parr CallumJC Hayashi Karin Kashida Shunnichi Hotta Akitsu Woltjen Knut Saito Hirohide
Nucleic Acids Research 2017-05-01
The CRISPR-Cas9 system is a powerful genome-editing tool useful in a variety of biotechnology and biomedical applications. Here we developed a synthetic RNA-based, microRNA (miRNA)-responsive CRISPR-Cas9 system (miR-Cas9 switch) in which the genome editing activity of Cas9 can be modulated through endogenous miRNA signatures in ...Read More
Yao Xuan Wang Xing Hu Xinde Liu Zhen Liu Junlai Zhou Haibo Shen Xiaowen Wei Yu Huang Zijian Ying Wenqin Wang Yan Nie Yan-Hong Zhang Chen-Chen Li Sanlan Cheng Leping Wang Qifang Wu Yan Huang Pengyu Sun Qiang Shi Linyu Yang Hui
Cell Research 2017-05-01
Targeted integration of transgenes can be achieved by strategies based on homologous recombination (HR), microhomology-mediated end joining (MMEJ) or non-homologous end joining (NHEJ). The more generally used HR is inefficient for achieving gene integration in animal embryos and tissues, because it occurs only during cell divisi...Read More
Wan Shuzhen Li Chaofeng Ma Xiaodong Luo Keming
Plant Cell Reports 2017-05-01
A novel R2R3 MYB transcription factor PtrMYB57 interacted with bHLH131 and PtrTTG1 to form the MBW complex and negatively regulated the biosynthesis of both anthocyanins and PAs in poplar. R2R3-MYB transcription factors (TFs) are important regulators of secondary metabolite biosynthesis in woody species. A series of R2R3-MYB TFs...Read More
Satomura Atsushi Nishioka Ryosuke Mori Hitoshi Sato Kosuke Kuroda Kouichi Ueda Mitsuyoshi
Scientific reports 2017-05-01
The CRISPR/Cas9 system has been applied to efficient genome editing in many eukaryotic cells. However, the bases that can be edited by this system have been limited to those within the protospacer adjacent motif (PAM) and guide RNA-targeting sequences. In this study, we developed a genome-wide base editing technology, CRISPR Nic...Read More
Cermak Tomas Curtin ShaunJ Gil-Humanes Javier ?egan Radim Kono ThomasJY Kone?n Eva Belanto JosephJ Starker ColbyG Mathre JadeW Greenstein RebeccaL Voytas DanielF
Plant Cell 2017-05-01
We report a comprehensive toolkit that enables targeted, specific modification of monocot and dicot genomes using a variety of genome engineering approaches. Our reagents, based on TALENs and the CRISPR/Cas9 system, are systematized for fast, modular cloning and accommodate diverse regulatory sequences to drive reagent expressio...Read More
Dong Xiaoyun Jin Yingli Ming Di Li Bangbang Dong Haisi Wang Lin Wang Tiedong Wang Dacheng
Journal of Microbiological Methods 2017-05-01
The understanding of the genetic mechanism of Staphylococcus aureus requires efficient tools, however, genetic manipulation in S. aureus is always laborious and time-consuming. Here we proposed a novel CRISPR/dCas9 interference method for the rapid knockdown of target genes. Furthermore, multiple genes can be repressed simultane...Read More
Yumlu Saniye Stumm Jrgen Bashir Sanum Dreyer Anne-Kathrin Lisowski Pawel Danner Eric Khn Ralf
Methods 2017-05-01
Human induced pluripotent stem cells (hiPSCs) represent an ideal in vitro platform to study human genetics and biology. The recent advent of programmable nucleases makes also the human genome amenable to experimental genetics through either the correction of mutations in patient-derived iPSC lines or the de novo introduction of ...Read More
Vanoli Fabio Jasin Maria
Methods 2017-05-01
Recurrent chromosomal translocations often lead to expression of fusion proteins associated with oncogenic transformation. To study translocations and downstream events, genome editing techniques have been developed to generate chromosomal translocations through non-homologous end joining of DNA double-strand breaks introduced a...Read More
Soppe JasperAdriaan Lebbink RobertJan
Trends in Microbiology 2017-05-01
The clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated (Cas) systems are RNA-guided sequence-specific prokaryotic antiviral immune systems. In prokaryotes, small RNA molecules guide Cas effector endonucleases to invading foreign genetic elements in a sequence-dependent manner, resulting in D...Read More
Kanda Yasukazu Yokotani Naoki Maeda Satoru Nishizawa Yoko Kamakura Takashi Mori Masaki
Bioscience, Biotechnology, and Biochemistry 2017-05-01
Broad-Spectrum Resistance 1 (BSR1) encodes a rice receptor-like cytoplasmic kinase, and enhances disease resistance when overexpressed. Rice plants overexpressing BSR1 are highly resistant to diverse pathogens, including rice blast fungus. However, the mechanism responsible for this resistance has not been fully characterized. T...Read More
Krivega Ivan Dean Ann
Nucleic Acids Research 2017-05-01
Mechanistic studies in erythroid cells indicate that LDB1, as part of a GATA1/TAL1/LMO2 complex, brings erythroid-expressed genes into proximity with enhancers for transcription activation. The role of co-activators in establishing this long-range interaction is poorly understood. Here we tested the contributions of the RNA Pol ...Read More
Adikusuma Fatwa Pederick Daniel McAninch Dale Hughes James Thomas Paul
Genetics 2017-05-01
Gene duplication provides spare genetic material that evolution can craft into new functions. Sox2 and Sox3 are evolutionarily-related genes with overlapping and unique sites of expression during embryogenesis. It is currently unclear whether SOX2 and SOX3 have identical or different functions. Here we use CRISPR/Cas9-assisted m...Read More
Liu Ying Conlon DonnaM Bi Xin Slovik KatherineJ Shi Jianting Edelstein HaileyI Millar JohnS Javaheri Ali Cuchel Marina Pashos EvanthiaE Iqbal Jahangir Hussain MMahmood Hegele RobertA Yang Wenli Duncan StephenA Rader DanielJ Morrisey EdwardE
Cell Reports 2017-05-01
Abetalipoproteinemia (ABL) is an inherited disorder of lipoprotein metabolism resulting from mutations in microsomal triglyceride transfer protein (MTTP). In addition to expression in the liver and intestine, MTTP is expressed in cardiomyocytes, and cardiomyopathy has been reported in several ABL cases. Using induced pluripotent...Read More
Ma Yuan Han Xin QuintanaBustamante Oscar BessadeCastro Ricardo Zhang Kai Zhang Pengchao Li Ying Liu Zongbin Liu Xuewu Ferrari Mauro Hu Zhongbo CarlosSegovia Jos Qin Lidong
Integrative Biology 2017-05-01
Recently, the clustered regularly interspaced short palindromic repeats (CRISPR)-Cas9 bacterial immunity system has opened a promising avenue to treat genetic diseases that affect the human hematopoietic stem cells (HSCs). Therefore, finding a highly efficient delivery method capable of modifying the genome in the hard-to-transf...Read More
Gao Mingming Liu Dexi
Cancer Biology & Therapy 2017-05-01
Both Pten and Nras are downstream mediators of receptor tyrosine kinase activation that plays important roles in controlling cell survival and proliferation. Here, we investigated whether and how Pten loss cross-talks with Nras activation in driving liver cancer development in mice. Somatic disruption of hepatic Pten and overexp...Read More
Li Ping Shi Ming-Lei Shen Wen-Long Zhang Zhang Xie De-Jian Zhang Xiang-Yuan He Chao Zhang Yan Zhao Zhi-Hu
Biochimica et Biophysica Acta 2017-05-01
Interferon-induced transmembrane protein (IFITM) 1, 2 and 3 are a family of interferon (IFN)-induced transmembrane proteins that block entry of a broad spectrum of pathogens. However, the transcriptional regulation of these genes, especially whether there exists any enhancers and their roles during the IFN induction process rema...Read More
Quadros RolenM Miura Hiromi Harms DonaldW Akatsuka Hisako Sato Takehito Aida Tomomi Redder Ronald Richardson GuyP Inagaki Yutaka Sakai Daisuke Buckley ShannonM Seshacharyulu Parthasarathy Batra SurinderK Behlke MarkA Zeiner SarahA Jacobi AshleyM Izu Yayoi Thoreson WallaceB Urness LisaD Mansour SuzanneL Ohtsuka Masato Gurumurthy ChannabasavaiahB
Genome Biology 2017-05-01
Conditional knockout mice and transgenic mice expressing recombinases, reporters, and inducible transcriptional activators are key for many genetic studies and comprise over 90% of mouse models created. Conditional knockout mice are generated using labor-intensive methods of homologous recombination in embryonic stem cells and a...Read More
McGee LynessaJ Jiang AlexL Lan Yu
Genesis 2017-05-01
Golgins are a family of coiled-coil proteins located at the cytoplasmic surface of the Golgi apparatus and have been implicated in maintaining Golgi structural integrity through acting as tethering factors for retrograde vesicle transport. Whereas knockdown of several individual golgins in cultured cells caused Golgi fragmentati...Read More
Cai Yupeng Chen Li Liu Xiujie Guo Chen Sun Shi Wu Cunxiang Jiang Bingjun Han Tianfu Hou Wensheng
Plant Biotechnology Journal 2017-05-01
Flowering is an indication of the transition from vegetative growth to reproductive growth and has considerable effects on the life cycle of soybean (Glycine max). In this study, we employed the CRISPR/Cas9 system to specifically induce targeted mutagenesis of GmFT2a, an integrator in the photoperiod flowering pathway in soybean...Read More
Dreissig Steven Schiml Simon Schindele Patrick Weiss Oda Rutten Twan Schubert Veit Gladilin Evgeny Mette MichaelFlorian Puchta Holger Houben Andreas
Plant Journal 2017-05-01
Elucidating the spatio-temporal organization of the genome inside the nucleus is imperative to understand the regulation of genes and non-coding sequences during development and environmental changes. Emerging techniques of chromatin imaging promise to bridge the long-standing gap between sequencing studies which reveal genomic ...Read More
Huai Cong Jia Chenqiang Sun Ruilin Xu Peipei Min Taishan Wang Qihan Zheng Chengde Chen Hongyan Lu Daru
Humangenetik 2017-05-01
Hemophilia B (HB) is an X-linked disorder caused by defects of F9 encoded coagulation factor IX, which is an ideal model for gene therapy. Most existing HB gene therapies are based on viral mediated gene supplementation, which could increase immunoreaction. In this study, CRISPR/Cas9 system was used for gene correction in an F9 ...Read More
Kang Xiangjin He Wenyin Huang Yuling Yu Qian Chen Yaoyong Gao Xingcheng Sun Xiaofang Fan Yong
Journal of Assisted Reproduction and Genetics 2017-05-01
Lee Bongyong Sahoo Anupama Marchica John Holzhauser Erwin Chen Xiaoli Li Jian-Liang Seki Tatsuya Govindarajan SubramaniamShyamala Markey FatuBadiane Batish Mona Lokhande SonaliJ Zhang Shaojie Ray Animesh Perera RanjanJ
Science advances 2017-05-01
Molecular mechanisms by which long noncoding RNA (lncRNA) molecules may influence cancerous condition are poorly understood. The aberrant expression of SPRIGHTLY lncRNA, encoded within the drosophila gene homolog Sprouty-4 intron, is correlated with a variety of cancers, including human melanomas. We demonstrate by SHAPE-seq and...Read More
Qin Zhiwei Munnoch JohnT Devine Rebecca Holmes NeilA Seipke RyanF Wilkinson KarlA Wilkinson Barrie Hutchings MatthewI
Chemical Science 2017-05-01
We report a new Streptomyces species named S. formicae that was isolated from the African fungus-growing plant-ant Tetraponera penzigi and show that it produces novel pentacyclic polyketides that are active against MRSA and VRE. The chemical scaffold of these compounds, which we have called the formicamycins, is similar to the f...Read More
Eskova Anastasia Chauvign Francois Maischein Hans-Martin Ammelburg Moritz Cerd Joan Nsslein-Volhard Christiane Irion Uwe
Development 2017-05-01
The development of the pigmentation pattern in zebrafish is a tightly regulated process that depends on both the self-organizing properties of pigment cells and extrinsic cues from other tissues. Many of the known mutations that alter the pattern act cell autonomously in pigment cells, and our knowledge about external regulators...Read More
Zhu LihuaJulie Lawrence Michael Gupta Ankit Pags Herv Kucukural Alper Garber Manuel Wolfe ScotA
BMC Genomics 2017-05-01
Genome editing technologies developed around the CRISPR-Cas9 nuclease system have facilitated the investigation of a broad range of biological questions. These nucleases also hold tremendous promise for treating a variety of genetic disorders. In the context of their therapeutic application, it is important to identify the spect...Read More
Young CourtneyS Mokhonova Ekaterina Quinonez Marbella Pyle AprilD Spencer MelissaJ
Journal of neuromuscular diseases 2017-05-01
Duchenne muscular dystrophy is caused by mutations in DMD which disrupt the reading frame. Therapeutic strategies that restore DMD's reading frame, such as exon skipping and CRISPR/Cas9, need to be tested in the context of the human DMD sequence in vivo. We have developed a novel dystrophic mouse model by using CRISPR/Cas9 to de...Read More
Stovicek Vratislav Holkenbrink Carina Borodina Irina
FEMS Yeast Research 2017-05-01
The methods based on the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/CRISPR-associated (Cas) system have quickly gained popularity for genome editing and transcriptional regulation in many organisms, including yeast. This review aims to provide a comprehensive overview of CRISPR application for different y...Read More
Peng Ying-Qian Tang Luo-Sheng Yoshida Shigeo Zhou Ye-Di
International journal of ophthalmology 2017-05-01
Gene therapy is a potentially effective treatment for retinal degenerative diseases. Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) system has been developed as a new genome-editing tool in ophthalmic studies. Recent advances in researches showed that CRISPR/Cas9 has been ap...Read More
Okada Masahiro Kanamori Mitsuhiro Someya Kazue Nakatsukasa Hiroko Yoshimura Akihiko
Epigenetics & Chromatin 2017-05-01
Epigenome editing is expected to manipulate transcription and cell fates and to elucidate the gene expression mechanisms in various cell types. For functional epigenome editing, assessing the chromatin context-dependent activity of artificial epigenetic modifier is required.Read More
Du Lijuan Zhou Amy Patel Akshay Rao Mishal Anderson Kelsey Roy Sougata
Developmental Biology 2017-05-01
Fibroblast growth factors (FGF) are essential signaling proteins that regulate diverse cellular functions in developmental and metabolic processes. In Drosophila, the FGF homolog, branchless (bnl) is expressed in a dynamic and spatiotemporally restricted pattern to induce branching morphogenesis of the trachea, which expresses t...Read More
Zhang Yunwei Bai Yang Wu Guangheng Zou Shenghao Chen Yongfang Gao Caixia Tang Dingzhong
Plant Journal 2017-05-01
Wheat (Triticum aestivum L.) incurs significant yield losses from powdery mildew, a major fungal disease caused by Blumeria graminis f. sp. tritici, (Bgt). ENHANCED DISEASE RESISTANCE1 (EDR1) plays a negative role in the defense response against powdery mildew in Arabidopsis thaliana; however, the edr1 mutant does not show const...Read More
Kao Pei-Hsun Ng I-Son
Bioresource Technology 2017-05-01
In this study, CRISPRi (clustered regularly interspaced short palindromic repeats interference) was used for the first time to regulate expression of exogenously supplied rfp gene as a proof-of-concept, and endogenous PEPC1 gene as a proof-of-function in Chlamydomonas reinhardtii. The efficiency of 94% and stability of 7 generat...Read More
Wassef M Luscan A Battistella A LeCorre S Li H Wallace MR Vidaud M Margueron R
Methods 2017-05-01
The advent of programmable nucleases such as ZFNs, TALENs and CRISPR/Cas9 has brought the power of genetic manipulation to widely used model systems. In mammalian cells, nuclease-mediated DNA double strand break is mainly repaired through the error-prone non-homologous end-joining (NHEJ) repair pathway, eventually leading to acc...Read More
Shen Ping Fan Jianzhong Guo Lihua Li Jiahua Li Ang Zhang Jing Ying Chaoqun Ji Jinru Xu Hao Zheng Beiwen Xiao Yonghong
Annals of Clinical Microbiology and Antimicrobials 2017-05-01
Shigellosis is the most common cause of gastrointestinal infections in developing countries. In China, the species most frequently responsible for shigellosis is Shigella flexneri. S. flexneri remains largely unexplored from a genomic standpoint and is still described using a vocabulary based on biochemical and serological prope...Read More
Wang Pengcheng Zhang Jun Sun Lin Ma Yizan Xu Jiao Liang Sijia Deng Jinwu Tan Jiafu Zhang Qinghua Tu Lili Daniell Henry Jin Shuangxia Zhang Xianlong
Plant Biotechnology Journal 2017-05-01
Gossypium hirsutum is an allotetraploid with a complex genome. Most genes have multiple copies that belong to At and Dt subgenomes. Sequence similarity is also very high between gene homologs. To efficiently achieve site/gene-specific mutation is quite needed. Due to its high efficiency and robustness, the CRISPR (Clustered Regu...Read More
Wang Xiaoli Zhang Wenmei Ding Yan Guo Xingrong Yuan Yahong Li Dongsheng
Oncology Reports 2017-05-01
CXC chemokine receptor 4 (CXCR4) is associated with poor clinical outcomes and decreased survival in hepatocellular carcinoma (HCC). In the present study, we targeted CXCR4 by CRISPR/Cas9 in HepG2 cells and observed the effects both invitro and invivo. The results indicated that after targeting CXCR4 the expression of CXCR4 was ...Read More
Kwon DeborahY Zhao Ying-Tao Lamonica JanineM Zhou Zhaolan
Nature Communications 2017-05-01
Efforts to manipulate locus-specific histone acetylation to assess their causal role in gene expression and cellular and behavioural phenotypes have been impeded by a lack of experimental tools. The Cas9 nuclease has been adapted to target epigenomic modifications, but a detailed description of the parameters of such synthetic e...Read More
Yan WinstonX Mirzazadeh Reza Garnerone Silvano Scott David Schneider MartinW Kallas Tomasz Custodio Joaquin Wernersson Erik Li Yinqing Gao Linyi Federova Yana Zetsche Bernd Zhang Feng Bienko Magda Crosetto Nicola
Nature Communications 2017-05-01
Precisely measuring the location and frequency of DNA double-strand breaks (DSBs) along the genome is instrumental to understanding genomic fragility, but current methods are limited in versatility, sensitivity or practicality. Here we present Breaks Labeling In Situ and Sequencing (BLISS), featuring the following: (1) direct la...Read More
Boyle EvanA Andreasson JohanOL Chircus LaurenM Sternberg SamuelH Wu MichelleJ Guegler ChantalK Doudna JenniferA Greenleaf WilliamJ
PNAS 2017-05-01
The bacterial adaptive immune system CRISPR-Cas9 has been appropriated as a versatile tool for editing genomes, controlling gene expression, and visualizing genetic loci. To analyze Cas9's ability to bind DNA rapidly and specifically, we generated multiple libraries of potential binding partners for measuring the kinetics of nuc...Read More
Yang Yanmei Lei Hong Qiang Ya-Wei Wang Bin
Molecular Biology of the Cell 2017-05-01
The anabolic action of PTH in bone is mostly mediated by cAMP/PKA and Wnt-independent activation of ?-catenin/T-cell factor (TCF) signaling. ?-catenin switches the PTH receptor (PTHR) signaling from cAMP/PKA to PLC/PKC activation by binding to the PTHR. Ixazomib (Izb) is the first orally administered proteasome inhibitor that wa...Read More
Banerjee Kalpita Keasey MattP Razskazovskiy Vladislav Visavadiya NishantP Jia Cuihong Hagg Theo
Cellular Signalling 2017-05-01
Excessive endoplasmic reticulum (ER) stress leads to cell loss in many diseases, e.g., contributing to endothelial cell loss after spinal cord injury. Here, we determined whether ER stress-induced mitochondrial dysfunction could be explained by interruption of the focal adhesion kinase (FAK)-mitochondrial STAT3 pathway we recent...Read More
Torres-Ruiz Raul Martinez-Lage Marta Martin MariaC Garcia Aida Bueno Clara Castao Julio Ramirez JuanC Menendez Pablo Cigudosa JuanC Rodriguez-Perales Sandra
Stem cell reports 2017-05-01
Efficient methodologies for recreating cancer-associated chromosome translocations are in high demand as tools for investigating how such events initiate cancer. The CRISPR/Cas9 system has been used to reconstruct the genetics of these complex rearrangements at native loci while maintaining the architecture and regulatory elemen...Read More
Yang Yu-Ping Ma Haiting Starchenko Alina Huh WonJae Li Wei Hickman FEdward Zhang Qin Franklin JeffreyL Mortlock DouglasP Fuhrmann Sabine Carter BruceD Ihrie RebeccaA Coffey RobertJ
Cell Reports 2017-05-01
EGF receptor (EGFR) is a critical signaling node throughout life. However, it has not been possible to directly visualize endogenous Egfr in mice. Using CRISPR/Cas9 genome editing, we appended a fluorescent reporter to the C terminus of the Egfr. Homozygous reporter mice appear normal and EGFR signaling is intact invitro and inv...Read More
Cai SW Zhang Y Hou MZ Liu Y Li XR
[Zhonghua yan ke za zhi] Chinese journal of ophthalmology 2017-05-01
Genome editing is a cutting-edge technology that generates DNA double strand breaks at the specific genomic DNA sequence through nuclease recognition and cleavage, and then achieves insertion, replacement, or deletion of the target gene via endogenous DNA repair mechanisms, such as non-homologous end joining, homology directed r...Read More
Ma Yijie Walsh MichaelJ Bernhardt Katharina Ashbaugh CamilleW Trudeau StephenJ Ashbaugh IsabelleY Jiang Sizun Jiang Chang Zhao Bo Root DavidE Doench JohnG Gewurz BenjaminE
Cell Host & Microbe 2017-05-01
Epstein-Barr virus (EBV) causes endemic Burkitt lymphoma (BL) and immunosuppression-related lymphomas. These B cell malignancies arise by distinct transformation pathways and have divergent viral and host expression programs. To identify hostdependency factors resulting from these EBV+,B cell-transformed cell states, we performe...Read More
Sefcikova Jana Roth Mitchell Yu Ge Li Hong
Bioessays 2017-05-01
RNA molecules are flexible yet foldable. Proteins must cope with this structural duality when forming biologically active complexes with RNA. Recent studies of the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPRs)-mediated RNA immunity illustrate some remarkable mechanisms with which proteins interact with RNA....Read More
Devi TakhellambamSwornalata Somayajulu Mallika Kowluru RenuAnjan Singh LalitPukhrambam
Cell Death and Disease 2017-05-01
Thioredoxin-interacting protein (TXNIP) is involved in oxidative stress and apoptosis in diabetic retinopathy. However, the role of TXNIP in the removal of damaged mitochondria (MT) via mitophagy, a process of macroautophagy, remains unexplored. Here we investigate the associated cellular and molecular mechanisms underlying mito...Read More
Fulcher LukeJ Hutchinson LukeD Macartney ThomasJ Turnbull Craig Sapkota GopalP
Open Biology 2017-05-01
Targeted proteolysis of endogenous proteins is desirable as a research toolkit and in therapeutics. CRISPR/Cas9-mediated gene knockouts are irreversible and often not feasible for many genes. Similarly, RNA interference approaches necessitate prolonged treatments, can lead to incomplete knockdowns and are often associated with o...Read More
Zeng Baosheng Huang Yuping Xu Jun Shiotsuki Takahiro Bai Hua Palli SubbaReddy Huang Yongping Tan Anjiang
Journal of Biological Chemistry 2017-05-01
Forkhead box O (FOXO) functions as the terminal transcription factor of the insulin signaling pathway and regulates multiple physiological processes in many organisms, including lifespan in insects. However, how FOXO interacts with hormone signaling to modulate insect growth and development is largely unknown. Here, using the tr...Read More
Liu Xue Gallay Clement Kjos Morten Domenech Arnau Slager Jelle vanKessel SebastiaanP Knoops Kvin Sorg RobinA Zhang Jing-Ren Veening Jan-Willem
Molecular Systems Biology 2017-05-01
Genome-wide screens have discovered a large set of essential genes in the opportunistic human pathogen Streptococcus pneumoniae However, the functions of many essential genes are still unknown, hampering vaccine development and drug discovery. Based on results from transposon sequencing (Tn-seq), we refined the list of essential...Read More
Cuculis Luke Schroeder CharlesM
Chemical and Biomolecular Engineering 2017-05-01
Exciting new advances in genome engineering have unlocked the potential to radically alter the treatment of human disease. In this review, we discuss the application of single-molecule techniques to uncover the mechanisms behind two premier classes of genome editing proteins: transcription activator-like effector nucleases (TALE...Read More
Sun Chao Jin Weiguo Shi Hongcan
International Journal of Molecular Medicine 2017-05-01
Oxidative signaling and oxidative stress contribute to aging, cancer and diseases resulting from lung fibrosis. In this study, we explored the anti-oxidative potential of oligomeric proanthocyanidins(OPCs), natural flavonoid compounds. We examined the protective effects of OPCs against hydrogen peroxide(H2O2)-induced oxidative s...Read More
Kampmann Martin
Chemical Communications 2017-05-01
Phenotypic screening is a powerful approach to discover small molecules with desired effects on biological systems, which can then be developed into therapeutic drugs. The identification of the target and mechanism of action of compounds discovered in phenotypic screens remains a major challenge. This feature article describes t...Read More
Marin MichelAbanto Fonseca Erica Encinas Fernando Freitas Fernanda Camargo DhianAlmeida Coimbra RoneySantos deFilippis Ivano Vicente AnaCarolina
Scientific reports 2017-05-01
Neisseria meningitidis infections are a major issue for global health. The invasive MenC ST-103 clonal complex (CC103) has been the most prevalent in meningococcal outbreaks in Brazil, occurring also in several countries worldwide. Here we have analysed the population structure and accessory genome of MenC CC103 strains from a g...Read More
Ohguchi H Harada T Sagawa M Kikuchi S Tai Y-T Richardson PG Hideshima T Anderson KC
Leukemia 2017-05-01
Recent studies have delineated cancer type-specific roles of histone 3 lysine 27 (H3K27) demethylase KDM6B/JMJD3 depending on its H3K27 demethylase activity. Here we show that KDM6B is expressed in multiple myeloma (MM); and that shRNA-mediated knockdown and CRISPR-mediated knockout of KDM6B abrogate MM cell growth and survival....Read More
Pankowicz FrancisP Jarrett KelseyE Lagor WilliamR Bissig Karl-Dimiter
Gut 2017-05-01
Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9 genome engineering has revolutionised biomedical science and we are standing on the cusp of medical transformation. The therapeutic potential of this technology is tremendous, however, its translation to the clinic will be challenging. In this article, we re...Read More
Chiurillo MiguelA Lander Noelia Bertolini MayaraS Storey Melissa Vercesi AnibalE Docampo Roberto
mBio 2017-05-01
Trypanosoma cruzi is the agent of Chagas disease, and the finding that this parasite possesses a mitochondrial calcium uniporter (TcMCU) with characteristics similar to that of mammalian mitochondria was fundamental for the discovery of the molecular nature of MCU in eukaryotes. We report here that ablation of TcMCU, or its para...Read More
Honghua Li Gang Liu
Hereditas (Beijing) 2017-05-01
Filamentous fungi usually refer to the eukaryotic microorganisms with developed mycelia and without large fruiting bodies. Filamentous fungi not only play an important role in material cycle in nature, but also are closely related to human health, industry and agriculture. However, the difficulties in genetic manipulation impede...Read More
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Nature Biotechnology 2017-05-01
Song Yuning Xu Yuxin Deng Jichao Chen Mao Lu Yi Wang Yong Yao Haobin Zhou Lina Liu Zhiquan Lai Liangxue Li Zhanjun
Scientific reports 2017-05-01
The 3' untranslated regions (UTRs), located at the end of mRNA molecules, are believed to play a role in RNA replication and/or protein translation. Mutations in the tyrosinase (Tyr) gene are known to cause recessive albinism in humans and other species. In this study, to test whether the CRISPR/Cas9 system works on the mutation...Read More
Nakamura Hidetoshi Katayama Takuya Okabe Tomoya Iwashita Kazuhiro Fujii Wataru Kitamoto Katsuhiko Maruyama Jun-Ichi
Journal of General and Applied Microbiology 2017-05-01
Numerous strains of Aspergillus oryzae are industrially used for Japanese traditional fermentation and for the production of enzymes and heterologous proteins. In A. oryzae, deletion of the ku70 or ligD genes involved in non-homologous end joining (NHEJ) has allowed high gene targeting efficiency. However, this strategy has been...Read More
Mekler Vladimir Minakhin Leonid Severinov Konstantin
PNAS 2017-05-01
The prokaryotic clustered regularly interspaced short palindromic repeats (CRISPR)-associated 9 (Cas9) endonuclease cleaves double-stranded DNA sequences specified by guide RNA molecules and flanked by a protospacer adjacent motif (PAM) and is widely used for genome editing in various organisms. The RNA-programmed Cas9 locates t...Read More
Dorighi KristelM Swigut Tomek Henriques Telmo Bhanu NatarajanV Scruggs BenjaminS Nady Nataliya Still ChristopherD Garcia BenjaminA Adelman Karen Wysocka Joanna
Molecular Cell 2017-05-01
Monomethylation of histone H3 at lysine 4 (H3K4me1) and acetylation of histone H3 at lysine 27 (H3K27ac) are correlated with transcriptionally engaged enhancer elements, but the functional impact of these modifications on enhancer activity isnot well understood. Here we used CRISPR/Cas9genome editing to separate catalytic activi...Read More
Du Dan Roguev Assen Gordon DavidE Chen Meng Chen Si-Han Shales Michael Shen JohnPaul Ideker Trey Mali Prashant Qi LeiS Krogan NevanJ
Nature Methods 2017-05-01
We describe a combinatorial CRISPR interference (CRISPRi) screening platform for mapping genetic interactions in mammalian cells. We targeted 107 chromatin-regulation factors in human cells with pools of either single or double single guide RNAs (sgRNAs) to downregulate individual genes or gene pairs, respectively. Relative enri...Read More
Cong Le
Analytical Biochemistry 2017-05-01
Years of advances in high-throughput biotechnologies exemplified by nucleic acid sequencing and single-molecular imaging have led to our increasing capacity to interrogate genomes down to nucleotide accuracy with single-cell or even subcellular resolution, thereby gaining high-dimensional information on the genetic variants and ...Read More
Yi Peishan Li Wen-Jun Dong Meng-Qiu Ou Guangshuo
Current Biology 2017-05-01
Cytoplasmic dynein-2 powers retrograde intraflagellar transport that is essential for cilium formation and maintenance. Inactivation of dynein-2 by mutations in DYNC2H1 causes skeletal dysplasias, and it remains unclear how the dynein-2 heavy chain moves in cilia. Here, using the genome-editing technique to produce fluorescent d...Read More
Bowman CaitlynE Rodriguez Susana SelenAlpergin EbruS Acoba MichelleG Zhao Liang Hartung Thomas Claypool StevenM Watkins PaulA Wolfgang MichaelJ
Cell Chemical Biology 2017-05-01
Malonyl-coenzyme A (malonyl-CoA) is a central metabolite in mammalian fatty acid biochemistry generated and utilized in the cytoplasm; however, little is known about noncanonical organelle-specificmalonyl-CoA metabolism. Intramitochondrial malonyl-CoA is generated by a malonyl-CoA synthetase, ACSF3, which produces malonyl-CoA fr...Read More
Rdis-Baptista Gandhi Campelo IanaS Morlighem Jean-tienneRL Melo LucianaM Freitas VicenteJF
Journal of Biotechnology 2017-05-01
Cell-penetrating peptides (CPPs) have been studied for their capacity to translocate across the lipid membrane of several cell types. In membrane translocation, these peptides can remarkably transport biologically active hydrophilic molecules, such as pharmaceuticals, nucleic acids (DNA and RNA) and even high-molecular-weight pr...Read More
Kampmann Martin
Trends in Molecular Medicine 2017-05-01
A major barrier to developing effective therapies for neurodegenerative diseases is our incomplete understanding of the underlying cellular mechanisms. Genetic screens in human-induced pluripotent stem cell-derived neurons can elucidate such mechanisms. Genome-wide screens using CRISPR interference and CRISPR activation provide ...Read More
Yao Xiaomin Liu Xiaowei Zhang Yaguang Li Yuhao Zhao Chengjian Yao Shaohua Wei Yu-Quan
Human Gene Therapy 2017-05-01
Adult-Onset Neuronal Ceroid Lipofuscinosis (ANCL), one of the neuronal ceroid lipofuscinosis (NCLs), is an inherited neurodegenerative disorder with progressive neuronal dysfunction. Recently, mutations in DNAJC5 gene that encodes Cysteine-String protein Alpha (CSP?) have been reported to be associated with familial Autosomal-Do...Read More
Collonnier Ccile Guyon-Debast Anouchka Maclot Franois Mara Kostlend Charlot Florence Nogu Fabien
Methods 2017-05-01
Beyond its predominant role in human and animal therapy, the CRISPR-Cas9 system has also become an essential tool for plant research and plant breeding. Agronomic applications rely on the mastery of gene inactivation and gene modification. However, if the knock-out of genes by non-homologous end-joining (NHEJ)-mediated repair of...Read More
Bisht Kamlesh Grill Sherilyn Graniel Jacqueline Nandakumar Jayakrishnan
Analytical Biochemistry 2017-05-01
CRISPR-Cas9 is a cutting-edge tool for modifying genomes. The efficacy with which Cas9 recognizes its target has revolutionized the engineering of knockouts. However this efficacy complicates the knocking out of important genes in cultured cells. Unedited cells holding a survival advantage within an edited population can confoun...Read More
East-Seletsky Alexandra O'Connell MitchellR Burstein David Knott GavinJ Doudna JenniferA
Molecular Cell 2017-05-01
CRISPR adaptive immunity pathways protect prokaryotic cells against foreign nucleic acids using CRISPR RNA (crRNA)-guided nucleases. In type VI-A CRISPR-Cas systems, the signature protein Cas13a (formerly C2c2) contains two separate ribonuclease activities that catalyze crRNA maturation and ssRNA degradation. The Cas13a protein ...Read More
Agrawal SmritiA Burgoyne Thomas Eblimit Aiden Bellingham James Parfitt DavidA Lane Amelia Nichols Ralph Asomugha Chinwe Hayes MatthewJ Munro PeterM Xu Mingchu Wang Keqing Futter ClareE Li Yumei Chen Rui Cheetham MichaelE
Human Molecular Genetics 2017-05-01
Retinitis pigmentosa (RP) is the most common form of inherited retinal dystrophy. We recently identified mutations in REEP6, which encodes the receptor expression enhancing protein 6, in several families with autosomal recessive RP. REEP6 is related to the REEP and Yop1p family of ER shaping proteins and potential receptor acces...Read More
Yuan Lin Yao Haobin Xu Yuxin Chen Mao Deng Jichao Song Yuning Sui Tingting Wang Yong Huang Yongye Li Zhanjun Lai Liangxue
Investigative Ophthalmology & Visual Science 2017-05-01
The present study aimed to investigate the role of the ?A-crystallin gene in inducing congenital cataracts in rabbits and to construct a novel animal model for characterization and pathologic analysis of congenital cataracts for future research.Read More
Long Shaojun Brown KevinM Drewry LisaL Anthony Bryan Phan IsabelleQH Sibley LDavid
PLoS Pathogens 2017-05-01
Toxoplasma gondii contains an expanded number of calmodulin (CaM)-like proteins whose functions are poorly understood. Using a combination of CRISPR/Cas9-mediated gene editing and a plant-like auxin-induced degron (AID) system, we examined the roles of three apically localized CaMs. CaM1 and CaM2 were individually dispensable, b...Read More
Lhdeniemi IrisAK Misiorek JuliaO Antila ChristianJM Landor SebastianK-J Stenvall Carl-GustafA Fortelius LinaE Bergstrm LindaK Sahlgren Cecilia Toivola DianaM
Cell Death and Differentiation 2017-05-01
Keratins (K) are intermediate filament proteins important in stress protection and mechanical support of epithelial tissues. K8, K18 and K19 are the main colonic keratins, and K8-knockout (K8(-/-)) mice display a keratin dose-dependent hyperproliferation of colonic crypts and a colitis-phenotype. However, the impact of the loss ...Read More
Tian Xiuzhi Wang Feng Zhang Lu Ji Pengyun Wang Jing Lv Dongying Li Guangdong Chai Menglong Lian Zhengxing Liu Guoshi
International Journal of Molecular Sciences 2017-05-01
CRISPR/Cas9 (Clustered regularly interspaced short palindromic repeats) combined with pronuclear microinjection has become the most effective method for producing transgenic animals. However, the relatively low embryo developmental rate limits its application. In the current study, it was observed that 10(-7) M melatonin is cons...Read More
Morgens DavidW Wainberg Michael Boyle EvanA Ursu Oana Araya CarlosL Tsui CKimberly Haney MichaelS Hess GaelenT Han Kyuho Jeng EdwinE Li Amy Snyder MichaelP Greenleaf WilliamJ Kundaje Anshul Bassik MichaelC
Nature Communications 2017-05-01
CRISPR-Cas9 screens are powerful tools for high-throughput interrogation of genome function, but can be confounded by nuclease-induced toxicity at both on- and off-target sites, likely due to DNA damage. Here, to test potential solutions to this issue, we design and analyse a CRISPR-Cas9 library with 10 variable-length guides pe...Read More
Fujita Toshitsugu Yuno Miyuki Suzuki Yutaka Sugano Sumio Fujii Hodaka
Genes to Cells 2017-05-01
Physical interactions between genomic regions play critical roles in the regulation of genome functions, including gene expression. Here, we show the feasibility of using engineered DNA-binding molecule-mediated chromatin immunoprecipitation (enChIP) in combination with next-generation sequencing (NGS) (enChIP-Seq) to detect suc...Read More
Wang William Wang Xiangdong
Cell Biology and Toxicology 2017-05-01
Higashioka Koki Koizumi Noriko Sakurai Hidetoshi Sotozono Chie Sato Takahiko
Stem cells international 2017-05-01
It is well known that myogenic regulatory factors encoded by the Myod1 family of genes have pivotal roles in myogenesis, with partially overlapping functions, as demonstrated for the mouse embryo. Myogenin-mutant mice, however, exhibit severe myogenic defects without compensation by other myogenic factors. MYOGENIN might be expe...Read More
Benston Shawna
Journal of Medical Ethics 2017-05-01
The ease and applicability of CRISPR/Cas9--a new and precise gene editing and reproductive technology--have garnered hype and heightened concern about its potential 'unprecedented and horrific consequences' and have led many scientific leaders to call for a moratorium on its research and use. CRISPR appears distinctly more contr...Read More
Romero-Moya Dami Santos-Ocaa Carlos Castao Julio Garrabou Gloria Rodrguez-Gmez JosA Ruiz-Bonilla Vanesa Bueno Clara Gonzlez-Rodriguez Patricia Giorgetti Alessandra Perdiguero Eusebio Prieto Cristina Moren-Nuez Constanza Fernndez-Ayala DanielJ Cascajo MariaVictoria Velasco Ivn Canals JosepMaria Montero Raquel Yubero Delia Jou Cristina Lpez-Barneo Jos Cardellach Francesc Muoz-Cnoves Pura Artuch Rafael Navas Plcido Menndez Pablo
Stem Cells 2017-05-01
Coenzyme Q10 (CoQ10 ) plays a crucial role in mitochondria as an electron carrier within the mitochondrial respiratory chain (MRC), and is an essential antioxidant. Mutations in genes responsible for CoQ10 biosynthesis (COQ genes) cause primary CoQ10 deficiency, a rare and heterogeneous mitochondrial disorder with no clear genot...Read More
Krupovic Mart Bguin Pierre Koonin EugeneV
Current Opinion in Microbiology 2017-05-01
A casposon, a member of a distinct superfamily of archaeal and bacterial self-synthesizing transposons that employ a recombinase (casposase) homologous to the Cas1 endonuclease, appears to have given rise to the adaptation module of CRISPR-Cas systems as well as the CRISPR repeats themselves. Comparison of the mechanistic featur...Read More
Gil MA Martinez CA Nohalez A Parrilla I Roca J Wu J Ross PJ Cuello C Izpisua JC Martinez EA
Molecular Reproduction and Development 2017-05-01
Genome editing in pigs has tremendous practical applications for biomedicine. The advent of genome editing technology, with its use of site-specific nucleases - including ZFNs, TALENs, and the CRISPR/Cas9 system - has popularized targeted zygote genome editing via one-step microinjection in several mammalian species. Here, we re...Read More
Mildner Alexander Chapnik Elik Varol Diana Aychek Tegest Lampl Nardi Rivkin Natalia Bringmann Anita Paul Franziska Boura-Halfon Sigalit Hayoun YifatSegal Barnett-Itzhaki Zohar Amit Ido Hornstein Eran Jung Steffen
European Journal of Immunology 2017-05-01
T-cell development is a spatially and temporally regulated process, orchestrated by well-defined contributions of transcription factors and cytokines. Here, we identify the non-coding RNA miR-142 as an additional regulatory layer within murine thymocyte development and proliferation. MiR-142 deficiency impairs the expression of ...Read More
Huang Z Tomitaka A Raymond A Nair M
Gene Therapy 2017-05-01
Human immunodeficiency virus (HIV)/acquired immunodeficiency syndrome (AIDS) remains a major health hazard despite significant advances in prevention and treatment of HIV infection. The major reason for the persistence of HIV/AIDS is the inability of existing treatments to clear or eradicate the multiple HIV reservoirs that exis...Read More
Shen Congle Liu Yongzhen Shi Shu Zhang Ruiyang Zhang Ting Xu Qiang Zhu Pengfei Chen Xiangmei Lu Fengmin
International Journal of Cancer 2017-05-01
Human papillomavirus (HPV) infection is the most important risk factor for cervical cancer development. In HeLa cell line, the HPV viral genome is integrated at 8q24 in one allele of chromosome 8. It has been reported that the HPV fragment integrated in HeLa genome can cis-activate the expression of proto-oncogene MYC, which is ...Read More
Cheishvili David Christiansen Steffan Stochinsky Rebecca Pepin Anne-Sophie Sapozhnikov DanielM Zhou Rudy Schmeltzer Lauren Dymov Sergey Szyf Moshe
Epigenomics 2017-05-01
DNA methylation downregulates transcription. However, a large number of genes, which are unmethylated in the promoter region, are inactive. We tested the hypothesis that these genes are regulated by DNA methylation of upstream regulators.Read More
Jiang Yu Qian Fenghui Yang Junjie Liu Yingmiao Dong Feng Xu Chongmao Sun Bingbing Chen Biao Xu Xiaoshu Li Yan Wang Renxiao Yang Sheng
Nature Communications 2017-05-01
Corynebacterium glutamicum is an important industrial metabolite producer that is difficult to genetically engineer. Although the Streptococcus pyogenes (Sp) CRISPR-Cas9 system has been adapted for genome editing of multiple bacteria, it cannot be introduced into C. glutamicum. Here we report a Francisella novicida (Fn) CRISPR-C...Read More
Si Tong Chao Ran Min Yuhao Wu Yuying Ren Wen Zhao Huimin
Nature Communications 2017-05-01
Genome-scale engineering is indispensable in understanding and engineering microorganisms, but the current tools are mainly limited to bacterial systems. Here we report an automated platform for multiplex genome-scale engineering in Saccharomyces cerevisiae, an important eukaryotic model and widely used microbial cell factory. S...Read More
Zou Zhong Huang Kun Wei Yanmin Chen Huanchun Liu Ziduo Jin Meilin
Scientific reports 2017-05-01
Duck enteritis virus (DEV), duck tembusu virus (DTMUV), and highly pathogenic avian influenza virus (HPAIV) H5N1 are the most important viral pathogens in ducks, as they cause significant economic losses in the duck industry. Development of a novel vaccine simultaneously effective against these three viruses is the most economic...Read More
Wu Junjun Zhang Xia Zhu Yingjie Tan Qinyu He Jiacheng Dong Mingsheng
Scientific reports 2017-05-01
Efficient biosynthesis of the plant polyphenol pinosylvin, which has numerous applications in nutraceuticals and pharmaceuticals, is necessary to make biological production economically viable. To this end, an efficient Escherichia coli platform for pinosylvin production was developed via a rational modular design approach. Init...Read More
Cortina Carme Turon Gemma Stork Diana Hernando-Momblona Xavier Sevillano Marta Aguilera Mnica Tosi Sbastien Merlos-Surez Anna Stephan-OttoAttolini Camille Sancho Elena Batlle Eduard
Embo Molecular Medicine 2017-05-01
The analysis of stem cell hierarchies in human cancers has been hampered by the impossibility of identifying or tracking tumor cell populations in an intact environment. To overcome this limitation, we devised a strategy based on editing the genomes of patient-derived tumor organoids using CRISPR/Cas9 technology to integrate rep...Read More
Kerros Celine Tripathi SatyendraC Zha Dongxing Mehrens JenniferM Sergeeva Anna Philips AnneV Qiao Na Peters HaleyL Katayama Hiroyuki Sukhumalchandra Pariya Ruisaard KathrynE Perakis AlexanderA StJohn LisaS Lu Sijie Mittendorf ElizabethA Clise-Dwyer Karen Herrmann AmandaC Alatrash Gheath Toniatti Carlo Hanash SamirM Ma Qing Molldrem JeffreyJ
Journal of Biological Chemistry 2017-05-01
Neutrophil elastase (NE) can be rapidly taken up by tumor cells that lack endogenous NE expression, including breast cancer, which results in cross-presentation of PR1, an NE-derived HLA-A2-restricted peptide that is an immunotherapy target in hematological and solid tumor malignancies. The mechanism of NE uptake, however, remai...Read More
Inoue Kimiko Hirose Michiko Inoue Hiroki Hatanaka Yuki Honda Arata Hasegawa Ayumi Mochida Keiji Ogura Atsuo
Cell Reports 2017-05-01
MicroRNAs (miRNAs) represent small noncoding RNAs that are involved in physiological and developmental processes by posttranscriptionally inhibiting gene expression. One of the largest miRNA clusters in mice is located in intron 10 of the Sfmbt2 gene, containing 72 miRNA precursor sequences. In this study, we generated mice lack...Read More
Zhang Han McCarty Nami
Journal of Cellular Biochemistry 2017-05-01
The revolutionary technology for genome editing known as the clustered regularly interspaced short palindromic repeat (CRISPR)-CRISPR-associated protein 9 (Cas9) system has sparked advancements in biological and biomedical research. The scientific breakthrough of the development of CRISPR-Cas9 technology has allowed us to recapi...Read More
Yu J Chen L Chen Y Hasan K Ghia EM Zhang L Wu R Rassenti LZ Widhopf GF Shen Z Briggs SP Kipps TJ
Leukemia 2017-05-01
Wnt5a can activate Rho GTPases in chronic lymphocytic leukemia cells by inducing the recruitment of ARHGEF2 to ROR1. Mass spectrometry on immune-precipitates of Wnt5a-activated ROR1 identified 14-3-3?, which was confirmed by co-immunoprecipitation. The capacity of Wnt5a to induce ROR1 to complex with 14-3-3? could be blocked in ...Read More
Huang Lei Hua Zaidong Xiao Hongwei Cheng Ying Xu Kui Gao Qian Xia Ying Liu Yang Zhang Xue Zheng Xinming Mu Yulian Li Kui
Oncotarget 2017-05-01
The traditional method to establish a cardiovascular disease model induced by high fat and high cholesterol diets is time consuming and laborious and may not be appropriate in all circumstances. A suitable pig model to study metabolic disorders and subsequent atherosclerosis is not currently available. For this purpose, we appli...Read More
Bulstrode Harry Johnstone Ewan Marques-Torrejon MariaAngeles Ferguson KirstyM Bressan RaulBardini Blin Carla Grant Vivien Gogolok Sabine Gangoso Ester Gagrica Sladjana Ender Christine Fotaki Vassiliki Sproul Duncan Bertone Paul Pollard StevenM
Genes & Development 2017-05-01
Glioblastoma multiforme (GBM) is an aggressive brain tumor driven by cells with hallmarks of neural stem (NS) cells. GBM stem cells frequently express high levels of the transcription factors FOXG1 and SOX2. Here we show that increased expression of these factors restricts astrocyte differentiation and can trigger dedifferentiat...Read More
Al-Jaff Mohammed Sandstrm Eric Grabherr Manfred
BMC Bioinformatics 2017-05-01
A common challenge in bioinformatics is to identify short sub-sequences that are unique in a set of genomes or reference sequences, which can efficiently be achieved by k-mer (k consecutive nucleotides) counting. However, there are several areas that would benefit from a more stringent definition of unique, requiring that these ...Read More
Hinze SJ Jackson MR Lie S Jolly L Field M Barry SC Harvey RJ Shoubridge C
Translational Psychiatry 2017-05-01
There is considerable genetic and phenotypic heterogeneity associated with intellectual disability (ID), specific learning disabilities, attention-deficit hyperactivity disorder, autism and epilepsy. The intelligence quotient (IQ) motif and SEC7 domain containing protein 2 gene (IQSEC2) is located on the X-chromosome and harbors...Read More
Lee Kunwoo Mackley VanessaA Rao Anirudh Chong AnthonyT Dewitt MarkA Corn JacobE Murthy Niren
eLife 2017-05-01
Chemical modification of the gRNA and donor DNA has great potential for improving the gene editing efficiency of Cas9 and Cpf1, but has not been investigated extensively. In this report, we demonstrate that the gRNAs of Cas9 and Cpf1, and donor DNA can be chemically modified at their terminal positions without losing activity. M...Read More
Ratiu JeremyJ Racine JeremyJ Hasham MuneerG Wang Qiming Branca JaneA Chapman HaroldD Zhu Jing Donghia Nina Philip Vivek Schott WilliamH Wasserfall Clive Atkinson MarkA Mills KevinD Leeth CarolineM Serreze DavidV
Journal of Immunology 2017-05-01
B lymphocytes play a key role in type 1 diabetes (T1D) development by serving as a subset of APCs preferentially supporting the expansion of autoreactive pathogenic T cells. As a result of their pathogenic importance, B lymphocyte-targeted therapies have received considerable interest as potential T1D interventions. Unfortunatel...Read More
VanSinay Elien Mirabeau Olivier Depuydt Geert VanHiel MatthiasBoris Peymen Katleen Watteyne Jan Zels Sven Schoofs Liliane Beets Isabel
PNAS 2017-05-01
In vertebrates thyrotropin-releasing hormone (TRH) is a highly conserved neuropeptide that exerts the hormonal control of thyroid-stimulating hormone (TSH) levels as well as neuromodulatory functions. However, a functional equivalent in protostomian animals remains unknown, although TRH receptors are conserved in proto- and deut...Read More
White MartynK Kaminski Rafal Young Won-Bin Roehm PamelaC Khalili Kamel
Journal of Cellular Biochemistry 2017-05-01
The CRISPR or clustered regularly interspaced short palindromic repeats system is currently the most advanced approach to genome editing and is notable for providing an unprecedented degree of specificity, effectiveness and versatility in genetic manipulation. CRISPR evolved as a prokaryotic immune system to confer provide an ac...Read More
Cameron Peter Fuller ChrisK Donohoue PaulD Jones BrittneeN Thompson MatthewS Carter MatthewM Gradia Scott Vidal Bastien Garner Elizabeth Slorach EuanM Lau Elaine Banh LyndaM Lied AlexandraM Edwards LeslieS Settle AlexanderH Capurso Daniel Llaca Victor Deschamps Stphane Cigan Mark Young JoshuaK May AndrewP
Nature Methods 2017-05-01
RNA-guided CRISPR-Cas9 endonucleases are widely used for genome engineering, but our understanding of Cas9 specificity remains incomplete. Here, we developed a biochemical method (SITE-Seq), using Cas9 programmed with single-guide RNAs (sgRNAs), to identify the sequence of cut sites within genomic DNA. Cells edited with the same...Read More
Tsai ShengdarQ Nguyen NhuT Malagon-Lopez Jose Topkar VedV Aryee MartinJ Joung JKeith
Nature Methods 2017-05-01
Sensitive detection of off-target effects is important for translating CRISPR-Cas9 nucleases into human therapeutics. In vitro biochemical methods for finding off-targets offer the potential advantages of greater reproducibility and scalability while avoiding limitations associated with strategies that require the culture and ma...Read More
Koehler KarlR Nie Jing Longworth-Mills Emma Liu Xiao-Ping Lee Jiyoon Holt JeffreyR Hashino Eri
Nature Biotechnology 2017-05-01
The derivation of human inner ear tissue from pluripotent stem cells would enable in vitro screening of drug candidates for the treatment of hearing and balance dysfunction and may provide a source of cells for cell-based therapies of the inner ear. Here we report a method for differentiating human pluripotent stem cells to inne...Read More
Roper Jatin Tammela Tuomas Cetinbas NaniyeMalli Akkad Adam Roghanian Ali Rickelt Steffen Almeqdadi Mohammad Wu Katherine Oberli MatthiasA Snchez-Rivera Francisco Park YoonaK Liang Xu Eng George Taylor MartinS Azimi Roxana Kedrin Dmitriy Neupane Rachit Beyaz Semir Sicinska EwaT Suarez Yvelisse Yoo James Chen Lillian Zukerberg Lawrence Katajisto Pekka Deshpande Vikram Bass AdamJ Tsichlis PhilipN Lees Jacqueline Langer Robert Hynes RichardO Chen Jianzhu Bhutkar Arjun Jacks Tyler Yilmaz merH
Nature Biotechnology 2017-05-01
In vivo interrogation of the function of genes implicated in tumorigenesis is limited by the need to generate and cross germline mutant mice. Here we describe approaches to model colorectal cancer (CRC) and metastasis, which rely on in situ gene editing and orthotopic organoid transplantation in mice without cancer-predisposing ...Read More
Brunger JonathanM Zutshi Ananya Willard VincentP Gersbach CharlesA Guilak Farshid
Stem cell reports 2017-05-01
Chronic inflammatory diseases such as arthritis are characterized by dysregulated responses to pro-inflammatory cytokines such as interleukin-1 (IL-1) and tumor necrosis factor ? (TNF-?). Pharmacologic anti-cytokine therapies are often effective at diminishing this inflammatory response but have significant side effects and are ...Read More
Hensel KaiO Rendon JulioC Navas Maria-Cristina Rots MarianneG Postberg Jan
FEBS Journal 2017-04-01
Worldwide, chronic hepatitis B virus (HBV) infection is a major health problem and no cure exists. Importantly, hepatocyte intrusion by HBV particles results in a complex deregulation of both viral and host cellular genetic and epigenetic processes. Among the attempts to develop novel therapeutic approaches against HBV infection...Read More
Yuen Kit-San Wang Zhong-Min Wong Nok-HeiMickey Zhang Zhi-Qian Cheng Tsz-Fung Lui Wai-Yin Chan Chi-Ping Jin Dong-Yan
Virus Research 2017-04-01
Epstein-Barr virus (EBV) infects more than 90% of the world's adult population. Once established, latent infection of nasopharyngeal epithelial cells with EBV is difficult to eradicate and might lead to the development of nasopharyngeal carcinoma (NPC) in a small subset of individuals. In this study we explored the anti-EBV pote...Read More
Molina-Snchez Pedro Lujambio Amaia
Aging (Albany, NY. Online) 2017-04-01
Sugano ShigeoS Suzuki Hiroko Shimokita Eisuke Chiba Hirofumi Noji Sumihare Osakabe Yuriko Osakabe Keishi
Scientific reports 2017-04-01
Mushroom-forming basidiomycetes produce a wide range of metabolites and have great value not only as food but also as an important global natural resource. Here, we demonstrate CRISPR/Cas9-based genome editing in the model species Coprinopsis cinerea. Using a high-throughput reporter assay with cryopreserved protoplasts, we iden...Read More
Chen Kai-Yuan Srinivasan Tara Tung Kuei-Ling Belmonte JulioM Wang Lihua Murthy PreetishKadurLakshminarasimha Choi Jiahn Rakhilin Nikolai King Sarah Varanko AnastasiaKristine Witherspoon Mavee Nishimura Nozomi Glazier JamesA Lipkin StevenM Bu Pengcheng Shen Xiling
Molecular Systems Biology 2017-04-01
The intestinal epithelium is the fastest regenerative tissue in the body, fueled by fast-cycling stem cells. The number and identity of these dividing and migrating stem cells are maintained by a mosaic pattern at the base of the crypt. How the underlying regulatory scheme manages this dynamic stem cell niche is not entirely cle...Read More
Cai Huajie Xie Xuemeng Ji Ling Ruan Xiaojiao Zheng Zhihai
Oncology Letters 2017-04-01
Sphingosine kinase 1 (Sphk1) is an oncogenic kinase that is responsible for the phosphorylation of sphingosine to sphingosine-1-phosphate (S1P). Mounting evidence suggests that Sphk1 serves a crucial role in the proliferation and development of a variety of human cancer cells. However, the role of Sphk1 in hepatocellular carcino...Read More
Nakamae Kazuki Nishimura Yuki Takenaga Mitsumasa Nakade Shota Sakamoto Naoaki Ide Hiroshi Sakuma Tetsushi Yamamoto Takashi
Bioengineered 2017-04-01
The emerging genome editing technology has enabled the creation of gene knock-in cells easily, efficiently, and rapidly, which has dramatically accelerated research in the field of mammalian functional genomics, including in humans. We recently developed a microhomology-mediated end-joining-based gene knock-in method, termed the...Read More
Kretzmann JessicaA Ho Diwei Evans CameronW Plani-Lam JaniceHC Garcia-Bloj Benjamin Mohamed AElaaf O'Mara MeganL Ford Ethan Tan DennisEK Lister Ryan Blancafort Pilar Norret Marck Iyer KSwaminathan
Chemical Science 2017-04-01
Tools for editing the genome and epigenome have revolutionised the field of molecular biology and represent a new frontier in targeted therapeutic intervention. Although efficiencies and specificities of genome editing technologies have improved with the development of TALEs and CRISPR platforms, intracellular delivery of these ...Read More
Tian Hongyu Ketova Tatiana Hardy Duriel Xu Xiaojiang Gao Xia Zijlstra Andries Blobe GerardC
Arteriosclerosis, Thrombosis, and Vascular Biology 2017-04-01
Endoglin, a transforming growth factor-? superfamily coreceptor, is predominantly expressed in endothelial cells and has essential roles in vascular development. However, whether endoglin is also expressed in vascular smooth muscle cells (VSMCs), especially in vivo, remains controversial. Furthermore, the roles of endoglin in VS...Read More
Karlgren Maria Simoff Ivailo Backlund Maria Wegler Christine Keiser Markus Handin Niklas Mller Janett Lundquist Patrik Jareborg Anne-Christine Oswald Stefan Artursson Per
Journal of Pharmaceutical Sciences 2017-04-01
Madin-Darby canine kidney (MDCK) II cells stably transfected with transport proteins are commonly used models for drug transport studies. However, endogenous expression of especially canine MDR1 (cMDR1) confounds the interpretation of such studies. Here we have established an MDCK cell line stably overexpressing the human MDR1 t...Read More
Anton Tobias Bultmann Sebastian
Nucleus (Austin, Tex. : Print) 2017-04-01
Dissecting the complex network of epigenetic modifications requires tools that combine precise recognition of DNA sequences with the capability to modify epigenetic marks. The CRISPR/Cas system has been proven to be a valuable addition to existing methodologies that fulfill these tasks. So far, sequence-specific editing of epige...Read More
Kamoshita Maki Kato Tsubasa Fujiwara Katsuyoshi Namiki Takafumi Matsumura Kazuaki Hyon Suong-Hyu Ito Junya Kashiwazaki Naomi
PLoS ONE 2017-04-01
Vitrification is a powerful tool for the efficient production of offspring derived from cryopreserved oocytes or embryos in mammalian species including domestic animals. Genome editing technologies such as transcription activator-like effector nucleases (TALENs) and clustered regularly interspaced short palindromic repeats (CRIS...Read More
Dong De Guo Minghui Wang Sihan Zhu Yuwei Wang Shuo Xiong Zhi Yang Jianzheng Xu Zengliang Huang Zhiwei
Nature 2017-04-01
The CRISPR-Cas9 systems are bacterial encoded adaptive immune systems to defend against phages infection, through RNA-guided endonuclease activity of Cas9 to degrade double-stranded DNA bearing a protospacer adjacent motif (PAM) and complementary sequences to the guide RNA1,2,3,4,5. Recently, two anti-CRISPR proteins AcrIIA2 and...Read More
Ramlee MuhammadKhairul Wang Jing Cheung AliceMS Li Shang
Journal of Visualized Experiments 2017-04-01
The development of programmable genome-editing tools has facilitated the use of reverse genetics to understand the roles specific genomic sequences play in the functioning of cells and whole organisms. This cause has been tremendously aided by the recent introduction of the CRISPR/Cas9 system-a versatile tool that allows researc...Read More
Crespillo-Casado Ana Chambers JosephE Fischer PeterM Marciniak StefanJ Ron David
eLife 2017-04-01
Dephosphorylation of translation initiation factor 2 (eIF2?) terminates signalling in the mammalian integrated stress response (ISR) and has emerged as a promising target for modifying the course of protein misfolding diseases. The [(o-chlorobenzylidene)amino]guanidines (Guanabenz and Sephin1) have been proposed to exert protect...Read More
Fuller MatthewS Majumder Kinjal Pintel DavidJ
Journal of Virology 2017-04-01
Replication of minute virus of mice (MVM) induces a sustained cellular DNA damage response (DDR) which the virus then exploits to prepare the nuclear environment for effective parvovirus takeover. An essential aspect of the MVM-induced DDR is the establishment of a potent pre-mitotic block, which we previously found to be indepe...Read More
Kim HeonSeok Lee Kyungjin Bae Sangsu Park Jeongbin Lee Chong-Kyo Kim Meehyein Kim Eunji Kim Minju Kim Seokjoong Kim Chonsaeng Kim Jin-Soo
Journal of Biological Chemistry 2017-04-01
Several groups have used genome-wide libraries of lentiviruses encoding small-guide RNAs (sgRNAs) for genetic screens. In most cases, sgRNA expression cassettes are integrated into cells by using lentiviruses, and target genes are statistically estimated by the readout of sgRNA sequences after targeted sequencing. We present a n...Read More
Crews Stephen
Genes & Development 2017-04-01
Cell signaling plays an essential role in development, and knowledge of the identities of the cells sending the signal is critical. This can be a challenge, since signaling pathways and ligands are commonly used at multiple times and in multiple cell types during development. One solution to this problem is to create cell type-s...Read More
Liu Yu Zhu Heng Jiang Xiao-Xia Zhang Bin Liao Li Liu Yuan-Lin Zhang Yi Chen Hu Mao Ning
Zhongguo shi yan xue ye xue za zhi / Zhongguo bing li sheng li xue hui = Journal of experimental hematology / Chinese Association of Pathophysiology 2017-04-01
To aimed at the establishment of mouse stably knockout of MYSM1 mesenchymal stem cell(MSC) line C3H10T1/2, and to investigate its immunological capacity of MSC in vitro.Read More
Ashdown GeorgeW Burn GarthL Williamson DavidJ Pand?i? Elvis Peters Ruby Holden Michael Ewers Helge Shao Lin Wiseman PaulW Owen DylanM
Biophysical Journal 2017-04-01
The cortical actin cytoskeleton has been shown to be critical for the reorganization and heterogeneity of plasma membrane components of many cells, including Tcells. Building on previous studies at the Tcell immunological synapse, we quantitatively assess the structure and dynamics of this meshwork using live-cell superresolutio...Read More
Strezoska ?aklina Perkett MatthewR Chou EldonT Maksimova Elena Anderson EmilyM McClelland Shawn Kelley MelissaL Vermeulen Annaleen Smith AnjavanBrabant
Journal of Biotechnology 2017-04-01
The CRISPR-Cas9 system has been utilized for large-scale, loss-of-function screens mainly using lentiviral pooled formats and cell-survival phenotypic assays. Screening in an arrayed format expands the types of phenotypic readouts that can be used to now include high-content, morphology-based assays, and with the recent availabi...Read More
Lee BeckyH Neela PoornimaH Kent MichaelS Zehnder AshleyM
PLoS ONE 2017-04-01
Canine oral mucosal melanoma is an aggressive malignant neoplasm and is characterized by local infiltration and a high metastatic potential. The disease progression is similar to that of human oral melanomas. Whereas human cutaneous melanoma is primarily driven by activating mutations in Braf (60%) or Nras (20%), human mucosal m...Read More
Hamacher Rainer Bauer Sebastian
Current Opinion in Oncology 2017-04-01
Sarcoma is a basket term for mesenchymal tumors for which more than 75 genetically and histologically distinct subtypes are recognized. Therapeutic progress has largely been achieved with classical chemotherapeutic drugs that were tested in empirical clinical trials. However, outcome in metastatic patients remains poor and with ...Read More
Song Yuning Liu Tingjun Wang Yong Deng Jichao Chen Mao Yuan Lin Lu Yi Xu Yuxin Yao Haobin Li Zhanjun Lai Liangxue
Oncotarget 2017-04-01
Sex-determining region Y is a crucial gene that initiates male sex determination in mammals. Mutations of the Sp1-binding site in the 5' flanking region of SRY are associated with clinical male-to-female sex reversal syndrome, although such occurrences are rare and, until now, have not been reported in animal models. In this stu...Read More
Wang Lijun Ran Lingyu Hou Yisu Tian Qiaoyan Li Chaofeng Liu Rui Fan Di Luo Keming
New Phytologist 2017-04-01
Proanthocyanidins (PAs) are major defense phenolic compounds in the leaves of poplar (Populus spp.) in response to abiotic and biotic stresses. Transcriptional regulation of PA biosynthetic genes by the MYB-basic helix-loop-helix (bHLH)-WD40 complexes in poplar is not still fully understood. Here, an Arabidopsis TT2-like gene MY...Read More
Aznar-Moreno JA Durrett TP
Plant and Cell Physiology 2017-04-01
The ability to easily transform Camelina sativa with biosynthetic enzymes derived from other plants has made this oil seed crop an ideal platform for the production of unusual lipids valuable for different applications. However, in addition to expressing transgenic enzymes, the suppression of endogenous enzyme activity to reduce...Read More
Midic Uros Hung Pei-Hsuan Vincent KaileyA Goheen Benjamin Schupp PatrickG Chen DianeD Bauer DanielE VandeVoort CatherineA Latham KeithE
Human Molecular Genetics 2017-04-01
Gene editing technologies offer new options for developing novel biomedical research models and for gene and stem cell based therapies. However, applications in many species demand high efficiencies, specificity, and a thorough understanding of likely editing outcomes. To date, overall efficiencies, rates of off-targeting, and d...Read More
Zeng Haiyan Zhang Jumei Li Chensi Xie Tengfei Ling Na Wu Qingping Ye Yingwang
Scientific reports 2017-04-01
Mitchell JonathanK Midkiff BentleyR Israelow Benjamin Evans MatthewJ Lanford RobertE Walker ChristopherM Lemon StanleyM McGivern DavidR
mBio 2017-04-01
Many DNA tumor viruses promote cellular transformation by inactivating the critically important tumor suppressor protein p53. In contrast, it is not known whether p53 function is disrupted by hepatitis C virus (HCV), a unique, oncogenic RNA virus that is the leading infectious cause of liver cancer in many regions of the world. ...Read More
Krentz NicoleAJ vanHoof Dennis Li Zhongmei Watanabe Akie Tang Mei Nian Cuilan German MichaelS Lynn FrancisC
Developmental Cell 2017-04-01
During pancreatic development, proliferating pancreatic progenitors activate the proendocrine transcription factor neurogenin 3 (NEUROG3), exit the cell cycle, and differentiate into islet cells. The mechanisms that direct robust NEUROG3 expression within a subset of progenitor cells control the size of the endocrine population....Read More
Ichikawa HTravis Cooper JohnC Lo Leja Potter Jason Terns RebeccaM Terns MichaelP
PLoS ONE 2017-04-01
The CRISPR-Cas systems provide invader defense in a wide variety of prokaryotes, as well as technologies for many powerful applications. The Type III-A or Csm CRISPR-Cas system is one of the most widely distributed across prokaryotic phyla, and cleaves targeted DNA and RNA molecules. In this work, we have constructed modules of ...Read More
Rawle AdrianJ Rawle FionaE
Biochemistry and Molecular Biology Education 2017-04-01
Yeung AmyTY Hale Christine Lee AmyH Gill ErinE Bushell Wendy Parry-Smith David Goulding David Pickard Derek Roumeliotis Theodoros Choudhary Jyoti Thomson Nick Skarnes WilliamC Dougan Gordon Hancock RobertEW
Nature Communications 2017-04-01
Chlamydia trachomatis remains a leading cause of bacterial sexually transmitted infections and preventable blindness worldwide. There are, however, limited in vitro models to study the role of host genetics in the response of macrophages to this obligate human pathogen. Here, we describe an approach using macrophages derived fro...Read More
Song Minjung
Biotechnology Progress 2017-04-01
The CRISPR/Cas9 gene editing system was originally derived from the prokaryotic adaptive immune system mediated by Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) and CRISPR-associated proteins (Cas). The system has been successfully applied to genome editing in eukaryotes and has contributed to remarkable adv...Read More
Solvsten ChristinaAE Daugaard TinaF Luo Yonglun dePaoli Frank Christensen JaneH Nielsen AndersL
Journal of Molecular Neuroscience 2017-04-01
Physical exercise results in the increased expression of neurotrophic factors and the subsequent induction of signal transduction cascades with a positive impact on neuronal functions. In this study, we used a voluntary physical exercise rat model to determine correlations in hippocampus mRNA expression of the neurotropic factor...Read More
Zhang Yu Long Chengzu Li Hui McAnally JohnR Baskin KedrynK Shelton JohnM Bassel-Duby Rhonda Olson EricN
Science advances 2017-04-01
Duchenne muscular dystrophy (DMD), caused by mutations in the X-linked dystrophin gene (DMD), is characterized by fatal degeneration of striated muscles. Dilated cardiomyopathy is one of the most common lethal features of the disease. We deployed Cpf1, a unique class 2 CRISPR (clustered regularly interspaced short palindromic re...Read More
Rollins MaryClareF Chowdhury Saikat Carter Joshua Golden SarahM Wilkinson RoyceA Bondy-Denomy Joseph Lander GabrielC Wiedenheft Blake
PNAS 2017-04-01
The type I-F CRISPR adaptive immune system in Pseudomonas aeruginosa (PA14) consists of two CRISPR loci and six CRISPR-associated (cas) genes. Type I-F systems rely on a CRISPR RNA (crRNA)-guided surveillance complex (Csy complex) to bind foreign DNA and recruit a trans-acting nuclease (i.e., Cas2/3) for target degradation. In m...Read More
Huang Weihua Wang Guiqing Sebra Robert Zhuge Jian Yin Changhong Aguero-Rosenfeld MariaE Schuetz AudreyN Dimitrova Nevenka Fallon JohnT
Antimicrobial Agents and Chemotherapy 2017-04-01
The extended-spectrum ?-lactamase (ESBL)- and Klebsiella pneumoniae carbapenemase (KPC)-producing Enterobacteriaceae represent serious and urgent threats to public health. In a retrospective study of multidrug-resistant K. pneumoniae, we identified three clinical isolates, CN1, CR14 and NY9, carrying both blaCTX-M and blaKPC gen...Read More
Ledford KelleyL Martinez-DeLuna ReynaI Theisen MatthewA Rawlins KarisaD Viczian AndreaS Zuber MichaelE
Developmental Biology 2017-04-01
The eye field transcription factor, Six6, is essential for both the early (specification and proliferative growth) phase of eye formation, as well as for normal retinal progenitor cell differentiation. While genomic regions driving six6 optic cup expression have been described, the sequences controlling eye field and optic vesic...Read More
Shaikh MushfiqH Clarke DanielTW Johnson NewellW McMillan NigelAJ
Oral Oncology 2017-04-01
Conventional treatment strategies have done little to improve the prognosis or disease-free survival in head and neck cancer (HNC) patients. Recent progress in our understanding of molecular aspects of head and neck squamous cell carcinoma (HNSCC) has provided insights into the potential use of molecular targeted therapies in co...Read More
Zerbini Francesca Zanella Ilaria Fraccascia Davide Knig Enrico Irene Carmela Frattini LucaF Tomasi Michele Fantappi Laura Ganfini Luisa Caproni Elena Parri Matteo Grandi Alberto Grandi Guido
Microbial Cell Factories 2017-04-01
The exploitation of the CRISPR/Cas9 machinery coupled to lambda (?) recombinase-mediated homologous recombination (recombineering) is becoming the method of choice for genome editing in E. coli. First proposed by Jiang and co-workers, the strategy has been subsequently fine-tuned by several authors who demonstrated, by using few...Read More
Badia Roger Ballana Ester Est JosA Riveira-Muoz Eva
Current Opinion in Virology 2017-04-01
The ability of some viruses to establish latently infected chronic reservoirs that escape to immune control becomes a major roadblock that impedes the cure of these infections. Therefore, new alternatives are needed to pursuit the eradication of viral persistent infections. Gene silencing technologies are in constant evolution a...Read More
Tangprasertchai NarinS DiFelice Rosa Zhang Xiaojun Slaymaker IanM VazquezReyes Carolina Jiang Wei Rohs Remo Qin PeterZ
ACS Chemical Biology 2017-04-01
The RNA-guided CRISPR-Cas9 nuclease has revolutionized genome engineering, yet its mechanism for DNA target selection is not fully understood. A crucial step in Cas9 target recognition involves unwinding of the DNA duplex to form a three-stranded R-loop structure. Work reported here demonstrates direct detection of Cas9-mediated...Read More
Bhattacharya Sujoy Yin Jinggang Winborn ChristinaS Zhang Qiuhua Yue Junming Chaum Edward
Investigative Ophthalmology & Visual Science 2017-04-01
Prominin-1 (Prom1) is a transmembrane glycoprotein, which is expressed in stem cell lineages, and has recently been implicated in cancer stem cell survival. Mutations in the Prom1 gene have been shown to disrupt photoreceptor disk morphogenesis and cause an autosomal dominant form of Stargardt-like macular dystrophy (STGD4). Des...Read More
Uehara Taisuke Minoshima Yukinori Sagane Koji Sugi NaokoHata Mitsuhashi KaoruOgawa Yamamoto Noboru Kamiyama Hiroshi Takahashi Kentaro Kotake Yoshihiko Uesugi Mai Yokoi Akira Inoue Atsushi Yoshida Taku Mabuchi Miyuki Tanaka Akito Owa Takashi
Nature Chemical Biology 2017-04-01
Target-protein degradation is an emerging field in drug discovery and development. In particular, the substrate-receptor proteins of the cullin-ubiquitin ligase system play a key role in selective protein degradation, which is an essential component of the anti-myeloma activity of immunomodulatory drugs (IMiDs), such as lenalido...Read More
Herold Nikolas Rudd SeanG Sanjiv Kumar Kutzner Juliane Bladh Julia Paulin CynthiaBJ Helleday Thomas Henter Jan-Inge Schaller Torsten
Cell Cycle 2017-04-01
Recently, we demonstrated that sterile ? motif and HD domain containing protein 1 (SAMHD1) is a major barrier in acute myelogenous leukemia (AML) cells to the cytotoxicity of cytarabine (ara-C), the most important drug in AML treatment. Ara-C is intracellularly converted by the canonical dNTP synthesis pathway to ara-CTP, which ...Read More
Fink JamesJ Robinson TiwannaM Germain NoelleD Sirois CarissaL Bolduc KaitlynA Ward AmandaJ Rigo Frank Chamberlain StormyJ Levine EricS
Nature Communications 2017-04-01
Angelman syndrome (AS) is a neurogenetic disorder caused by deletion of the maternally inherited UBE3A allele and is characterized by developmental delay, intellectual disability, ataxia, seizures and a happy affect. Here, we explored the underlying pathophysiology using induced pluripotent stem cell-derived neurons from AS pati...Read More
Noble Charleston Olejarz Jason Esvelt KevinM Church GeorgeM Nowak MartinA
Science advances 2017-04-01
The alteration of wild populations has been discussed as a solution to a number of humanity's most pressing ecological and public health concerns. Enabled by the recent revolution in genome editing, clustered regularly interspaced short palindromic repeats (CRISPR) gene drives-selfish genetic elements that can spread through pop...Read More
Ren Jiangtao Zhao Yangbing
Protein & Cell 2017-04-01
The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated 9 (CRISPR/Cas9) system, an RNA-guided DNA targeting technology, is triggering a revolution in the field of biology. CRISPR/Cas9 has demonstrated great potential for genetic manipulation. In this review, we discuss the current development of ...Read More
Jang Ye-Ji Seo Seung-Oh Kim Seul-Ah Li Ling Kim Tae-Jip Kim SunChang Jin Yong-Su Han NamSoo
Journal of Biotechnology 2017-04-01
Leuconostoc spp. are important lactic acid bacteria for the fermentation of foods. In particular, L. citreum strains isolated from various foods have been used as host strains for genetic and metabolic engineering studies. In order to develop a food-grade genetic engineering system, L. citreum CB2567 was isolated from Kimchi. Ho...Read More
Yang Wenqing Zhang Siliang Zhang Yi Huang Xin
Biochemical and Biophysical Research Communications 2017-04-01
Gene transcription is a central tenet of biology, traditionally measured by RT-PCR, microarray, or more recently, RNA sequencing. However, these measurements only provide a snapshot of the state of gene transcription and only represent an overall readout of complex transcriptional networks that regulate gene expression. In this ...Read More
Higashijima Yoshiki Hirano Seiichi Nangaku Masaomi Nureki Osamu
Kidney International 2017-04-01
The recently discovered clustered regularly interspaced short palindromic repeat (CRISPR)-CRISPR-associated protein 9 (Cas9) is an RNA-guided DNA nuclease, and has been harnessed for the development of simple, efficient, and relatively inexpensive technologies to precisely manipulate the genomic information in virtually all cell...Read More
Islam MdFahmid Watanabe Atsushi Wong Lai Lazarou Conor Vizeacoumar FrederickS Abuhussein Omar Hill Wayne Uppalapati Maruti Geyer CRonald Vizeacoumar FrancoJ
Scientific reports 2017-04-01
Next generation sequencing is becoming the method of choice for functional genomic studies that use pooled shRNA or CRISPR libraries. A key challenge in sequencing these mixed-oligo libraries is that they are highly susceptible to hairpin and/or heteroduplex formation. This results in polyclonal, low quality, and incomplete read...Read More
Ludman Mrta Burgyn Jzsef Ftyol Kroly
Scientific reports 2017-04-01
RNA silencing constitutes an important antiviral mechanism in plants. Small RNA guided Argonaute proteins fulfill essential role in this process by acting as executors of viral restriction. Plants encode multiple Argonaute proteins of which several exhibit antiviral activities. A recent addition to this group is AGO2. Its involv...Read More
Wallace JaredA Kagele DominiqueA Eiring AnnaM Kim CarissaN Hu Ruozhen Runtsch MarahC Alexander Margaret Huffaker ThomasB Lee Soh-Hyun Patel AmiB Mosbruger TimothyL Voth Warren Rao DineshS Miles RodneyR Round JuneL Deininger MichaelW O'Connell RyanM
Blood 2017-04-01
FLT3-ITD(+) AML accounts for approximately 25% of all AML cases, and is a subtype that carries a poor prognosis. miR-155 is specifically overexpressed in FLT3-ITD(+) AML compared to FLT3-WT AML, and is critical for the growth of FLT3-ITD(+) AML cells in vitro. However, miR-155's role in regulating FLT3-ITD-mediated disease in vi...Read More
Treiber Thomas Treiber Nora Plessmann Uwe Harlander Simone Dai Julia-Lisa Eichner Norbert Lehmann Gerhard Schall Kevin Urlaub Henning Meister Gunter
Molecular Cell 2017-04-01
During microRNA (miRNA) biogenesis, two endonucleolytic reactions convert stem-loop-structured precursors into mature miRNAs. These processing steps can be posttranscriptionally regulated by RNA-binding proteins (RBPs). Here, we have used aproteomics-based pull-down approach to map andcharacterize the interactome of a multitude ...Read More
Swarts DaanC vanderOost John Jinek Martin
Molecular Cell 2017-04-01
The CRISPR-associated protein Cas12a (Cpf1), which has been repurposed for genome editing, possesses two distinct nuclease activities: endoribonuclease activity for processing its own guide RNAs and RNA-guided DNase activity for target DNA cleavage. To elucidate the molecular basis of both activities, we determined crystal struc...Read More
Benedetti Lorena Cereda Matteo Monteverde LeeAnn Desai Nikita Ciccarelli FrancescaD
Oncotarget 2017-04-01
Cohesin is a multi-protein complex that tethers sister chromatids during mitosis and mediates DNA repair, genome compartmentalisation and regulation of gene expression. Cohesin subunits frequently acquire cancer loss-of-function alterations and act as tumour suppressors in several tumour types. This has led to increased interest...Read More
Demirci Yeliz Zhang Baohong Unver Turgay
Journal Of Cellular Physiology 2017-04-01
CRISPR/Cas 9 is a newly developed and naturally occurred genome editing tool, which is originally used by bacteria for immune defence. In the past years, it has been quickly employed and modified to precisely edit genome sequences in both plants and animals. Compared with the well-developed zinc finger nucleases (ZFNs) and trans...Read More
Wang Lingbo Li Min-Yin Qu Chao Miao Wan-Ying Yin Qi Liao Jiaoyang Cao Hua-Teng Huang Min Wang Kai Zuo Erwei Peng Guangdun Zhang Shu-Xin Chen Guodong Li Qing Tang Ke Yu Qian Li Zhoujie Wong CatherineCl Xu Guoliang Jing Naihe Yu Xiang Li Jinsong
Cell Research 2017-04-01
Studying the early function of essential genes is an important and challenging problem in developmental biology. Here, we established a method for rapidly inducing CRISPR-Cas9-mediated mutations in one blastomere of two-cell stage embryos, termed 2-cell embryo-CRISPR-Cas9 injection (2CC), to study the in vivo function of essenti...Read More
Zhu Jie Ming Chang Fu Xin Duan Yaou Hoang DucAnh Rutgard Jeffrey Zhang Runze Wang Wenqiu Hou Rui Zhang Daniel Zhang Edward Zhang Charlotte Hao Xiaoke Xiong Wenjun Zhang Kang
Cell Research 2017-04-01
Mitra Aparajita Sarin YogeshKumar
Indian Journal of Pediatrics 2017-04-01
Hypospadiology is a rapidly evolving field. Progress in the understanding of how hormonal therapy affects the growth of the phallus has allowed surgeons to optimize the tissues for surgery. But conflicting data from a number of studies and a lack of consensus on drugs, their dosing, mode of delivery and timing of use means that ...Read More
Rogers WilliamA Goyal Yogesh Yamaya Kei Shvartsman StanislavY Levine MichaelS
Genes & Development 2017-04-01
The EGF signaling pathway specifies neuronal identities in the Drosophila embryo by regulating developmental patterning genes such as intermediate neuroblasts defective (ind). EGFR is activated in the ventral midline and neurogenic ectoderm by the Spitz ligand, which is processed by the Rhomboid protease. CRISPR/Cas9 was used to...Read More
Bottanelli Francesca Kilian Nicole Ernst AndreasM Rivera-Molina Felix Schroeder LenaK Kromann EmilB Lessard MarkD Erdmann RomanS Schepartz Alanna Baddeley David Bewersdorf Joerg Toomre Derek Rothman JamesE
Molecular Biology of the Cell 2017-04-01
Capitalizing on CRISPR/Cas9 gene editing techniques and super-resolution nanoscopy, we have explored the role of the small GTPase ARF1 in mediating transport steps at the Golgi. Surprisingly, beside its well-established role in generating COPI vesicles, we find that ARF1 is additionally involved in the formation of long (? 3 ?m)...Read More
Takei Yodai Shah Sheel Harvey Sho Qi LeiS Cai Long
Biophysical Journal 2017-04-01
Visualization of chromosome dynamics allows the investigation of spatiotemporal chromatin organization and its role in gene regulation and other cellular processes. However, current approaches to label multiple genomic loci in live cells have a fundamental limitation in the number of loci that can be labeled and uniquely identif...Read More
Karlsen Christian Hjerde Erik Klemetsen Terje Willassen NilsPeder
BMC Genomics 2017-04-01
Winter-ulcer Moritella viscosa infections continue to be a significant burden in Atlantic salmon (Salmo salar L.) farming. M. viscosa comprises two main clusters that differ in genetic variation and phenotypes including virulence. Horizontal gene transfer through acquisition and loss of mobile genetic elements (MGEs) is a major ...Read More
Hong Gyu-Sang Lee Byeongjun Oh Uhtaek
Neuron 2017-04-01
Mechanosensation is essential for various physiological processes, and it is mediated by mechanotransduction channels. Recently, we reported that TMEM150C/Tentonin 3 (TTN3) confers mechanically activated currents with slow inactivation kinetics in several cell types, including dorsal root ganglion neurons (Hong etal., 2016). The...Read More
Dubin AdrienneE Murthy Swetha Lewis AmandaH Brosse Lucie Cahalan StuartM Grandl Jrg Coste Bertrand Patapoutian Ardem
Neuron 2017-04-01
A gold standard for characterizing mechanically activated (MA) currents is via heterologous expression ofcandidate channels in naive cells. Two recent studies described MA channels using this paradigm. TMEM150c was proposed to be a component of an MA channel partly based on a heterologous expression approach (Hong etal., 2016). ...Read More
Stemmer Manuel Thumberger Thomas DelSolKeyer Maria Wittbrodt Joachim Mateo JuanL
PLoS ONE 2017-04-01
[This corrects the article DOI: 10.1371/journal.pone.0124633.].Read More
Ali Qasim Wahl LindiM
Journal of Biological Dynamics 2017-04-01
Clustered regularly interspaced short palindromic repeats (CRISPR), linked with CRISPR associated (Cas) genes, can confer adaptive immunity to bacteria, against bacteriophage infections. Thus from a therapeutic standpoint, CRISPR immunity increases biofilm resistance to phage therapy. Recently, however, CRISPR-Cas genes have bee...Read More
Umenhoffer Kinga Draskovits Gbor Nyerges kos Karcagi Ildik Bogos Balzs Tmr Edit Csrg? Blint Herczeg Rbert Nagy Istvn Fehr Tams Pl Csaba Psfai Gyrgy
ACS Synthetic Biology 2017-04-01
The ideal bacterial chassis provides a simplified, stable and predictable host environment for synthetic biological circuits. Mutability and evolution can, however, compromise stability, leading to deterioration of artificial genetic constructs. By eliminating certain sources of instability, these undesired genetic changes can b...Read More
Quinn PeterM Pellissier LucieP Wijnholds Jan
Frontiers in Neuroscience 2017-04-01
Once considered science fiction, gene therapy is rapidly becoming scientific reality, targeting a growing number of the approximately 250 genes linked to hereditary retinal disorders such as retinitis pigmentosa and Leber's congenital amaurosis. Powerful new technologies have emerged, leading to the development of humanized mode...Read More
Li Ming Au LaurenYunCook Douglah Deema Chong Abigail White BradleyJ Ferree PatrickM Akbari OmarS
Scientific reports 2017-04-01
The revolutionary RNA-guided endonuclease CRISPR/Cas9 system has proven to be a powerful tool for gene editing in a plethora of organisms. Here, utilizing this system we developed an efficient protocol for the generation of heritable germline mutations in the parasitoid jewel wasp, Nasonia vitripennis, a rising insect model orga...Read More
Wakim Jamal Goudenege David Perrot Rodolphe Gueguen Naig Desquiret-Dumas Valerie delaBarca JuanManuelChao Rosa IlariaDalla Manero Florence LeMao Morgane Chupin Stephanie Chevrollier Arnaud Procaccio Vincent Bonneau Dominique Logan DavidC Reynier Pascal Lenaers Guy Khiati Salim
Journal of Cell Science 2017-04-01
Mitochondrial dynamics and distribution are critical in supplying ATP in response to energy demand. CLUH is a protein involved in mitochondrial distribution, whose dysfunction leads to mitochondrial clustering, the metabolic consequences of which remain unknown. To gain insight into the role of CLUH on mitochondrial energy produ...Read More
Kitazawa Satoshi Ebara Shunsuke Ando Ayumi Baba Yuji Satomi Yoshinori Soga Tomoyoshi Hara Takahito
Oncotarget 2017-04-01
Mutations in succinate dehydrogenase B (SDHB) gene are frequently observed in several tumors and associated with poor prognosis in these tumors. Therefore, drugs effective for SDHB-deficient tumors could fulfill an unmet medical need. In addition, such drugs would have an advantage in that selection of patients with SDHB-mutant ...Read More
Estoppey David Lee ChiaMin Janoschke Marco Lee BoonHeng Wan KahFei Dong Hongping Mathys Philippe Filipuzzi Ireos Schuhmann Tim Riedl Ralph Aust Thomas Galuba Olaf McAllister Gregory Russ Carsten Spiess Martin Bouwmeester Tewis Bonamy GhislainMC Hoepfner Dominic
Cell Reports 2017-04-01
Flavivirus infections by Zika and dengue virus impose a significant global healthcare threat with no US Food and Drug Administration (FDA)-approved vaccination or specific antiviral treatment available. Here, we present the discovery of an anti-flaviviral natural product named cavinafungin. Cavinafungin is a potent and selective...Read More
McCormack RyanM Szymanski EvaP Hsu AmyP Perez Elena Olivier KennethN Fisher Eva Goodhew EBrook Podack EckhardR Holland StevenM
JCI Insight 2017-04-01
Perforin-2 is a highly conserved pore-forming protein encoded by macrophage expressed gene 1 (MPEG1). A number of studies have shown that Perforin-2-deficient mice are unable to survive following a bacterial challenge that is nonlethal in WT mice. There is also recent evidence that Mpeg1+/- heterozygous mice display an intermedi...Read More
Park Mi-Young Jung MinHee Eo EunYoung Kim Seokjoong Lee SangHoon Lee YeonJoo Park JongSun Cho YoungJae Chung JinHaeng Kim CheolHyeon Yoon HoIl Lee JaeHo Lee Choon-Taek
Oncotarget 2017-04-01
Tyrosine kinase inhibitors (TKIs) such as gefitinib and erlotinib are effective against lung adenocarcinomas harboring epidermal growth factor receptor (EGFR) mutations. However, cancer cells can develop resistance to these agents with prolonged exposure; in over 50% of cases, this is attributable to the EGFR T790M mutation. Mor...Read More
Niccheri Francesca Pecori Riccardo Conticello SilvestroG
Experientia. Basel 2017-04-01
Clustered Regularly Interspaced Short Palindromic Repeats-associated protein 9 nuclease (CRISPR/Cas9) and Transcription Activator-Like Effector Nucleases (TALENs) are versatile tools for genome editing. Here we report a method to increase the frequency of Cas9-targeted cellular clones. Our method is based on a chimeric construct...Read More
Puschnik AndreasS Majzoub Karim Ooi YawShin Carette JanE
Nature Reviews: Microbiology 2017-04-01
Viruses depend on their hosts to complete their replication cycles; they exploit cellular receptors for entry and hijack cellular functions to replicate their genome, assemble progeny virions and spread. Recently, genome-scale CRISPR-Cas screens have been used to identify host factors that are required for virus replication, inc...Read More
Shuang Li Yuanyuan Yang Yan Qiu Yanhao Chen Luwei Xu Qiurong Ding
Hereditas (Beijing) 2017-04-01
The emergence of genome editing tools, such as the clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated 9 (Cas9) system, has enabled researchers to achieve somatic and germline genomic manipulations in cell lines and model organisms. Within a couple of years, genome editing is now being rapidly de...Read More
Leyang Wang Haiyan Huang Qiang Wu
Hereditas (Beijing) 2017-04-01
In complex genomes, there are a large number of duplicated genes in the coding regions and many more repetitive sequences in the non-coding regions. Repetitive sequences can exert great impacts on the heredity and evolution of the organisms, as well as their genome 3D architecture and transcriptional regulation. The high homolog...Read More
Yang Li Liu Ya'Nan Zhang Na Ding Xiao'Yi Zhang Wei Shen Ke'Feng Huang Liang Zhou Jian'Feng Cui Sen Zhu Zun'Min Hu Zheng Xiao Min
Oncotarget 2017-04-01
DNA methyltransferase 3A (DNMT3A) mutations occurred in 18%~23% of acute myeloid leukemia (AML) patients, and were considered to be an adverse prognostic factor for adult de novo AML cases. However, the relevant molecular mechanism of the mutation in AML pathogenesis remains obscure. In this study, we established K562 and SKM1 c...Read More
Hussmann Dianna Madsen AnneTranberg Jakobsen KristineRaaby Luo Yonglun Sorensen BoeSandahl Nielsen AndersLade
Oncotarget 2017-04-01
EGFR-mutated non-small cell lung cancer patients experience relapse within 1-2 years of treatment with EGFR-inhibitors, such as erlotinib. Multiple resistance mechanisms have been identified including secondary EGFR-mutations, MET-amplification, and epithelial-mesenchymal transition (EMT). Previous studies have indicated a role ...Read More
Shen Chih-Che Sung Li-Yu Lin Shih-Yeh Lin Mei-Wei Hu Yu-Chen
ACS Synthetic Biology 2017-04-01
Chinese hamster ovary (CHO) cells are an important host for biopharmaceutical production. Generation of stable CHO cells typically requires cointegration of dhfr and a foreign gene into chromosomes and subsequent methotrexate (MTX) selection for coamplification of dhfr and foreign gene. CRISPR interference (CRISPRi) is an emergi...Read More
Agudelo Daniel Duringer Alexis Bozoyan Lusin Huard CarolineC Carter Sophie Loehr Jeremy Synodinou Dafni Drouin Mathieu Salsman Jayme Dellaire Graham Laganire Jose Doyon Yannick
Nature Methods 2017-04-01
Targeted genome editing enables the creation of bona fide cellular models for biological research and may be applied to human cell-based therapies. Therefore, broadly applicable and versatile methods for increasing its efficacy in cell populations are highly desirable. We designed a simple and robust coselection strategy for enr...Read More
Li Bin Ding Siyuan Feng Ningguo Mooney Nancie Ooi YawShin Ren Lili Diep Jonathan Kelly MarcusR Yasukawa LindaL Patton JohnT Yamazaki Hiroyuki Shirao Tomoaki Jackson PeterK Greenberg HarryB
PNAS 2017-04-01
Despite the wide administration of several effective vaccines, rotavirus (RV) remains the single most important etiological agent of severe diarrhea in infants and young children worldwide, with an annual mortality of over 200,000 people. RV attachment and internalization into target cells is mediated by its outer capsid protein...Read More
Hua Yufeng Wang Chun Huang Jian Wang Kejian
Journal of Genetics and Genomics 2017-04-01
The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) system provides a technological breakthrough in mutant generation. Several methods such as the polymerase chain reaction (PCR)/restriction enzyme (RE) assay, T7 endonuclease I (T7EI) assay, Surveyor nuclease assay, PAGE-base...Read More
Xie Shiqi Duan Jialei Li Boxun Zhou Pei Hon GaryC
Molecular Cell 2017-04-01
The study of enhancers has been hampered by the scarcity of methods to systematically quantify their endogenous activity. We develop Mosaic-seq to systematically perturb enhancers and measure theirendogenous activities at single-cell resolution. Mosaic-seq uses a CRISPR barcoding system to jointly measure a cell's transcriptome ...Read More
Si Fangwei Li Dongyang Cox SarahE Sauls JohnT Azizi Omid Sou Cindy Schwartz AmyB Erickstad MichaelJ Jun Yonggun Li Xintian Jun Suckjoon
Current Biology 2017-04-01
It is generally assumed that the allocation and synthesis of total cellular resources in microorganisms are uniquely determined by the growth conditions. Adaptation to a new physiological state leads to a change in cell size via reallocation of cellular resources. However, it has not been understood how cell size is coordinated ...Read More
Liao Yunfei Chen Lulu Feng Yong Shen Jacson Gao Yan Cote Gregory Choy Edwin Harmon David Mankin Henry Hornicek Francis Duan Zhenfeng
Oncotarget 2017-04-01
Programmed cell death ligand 1 (PD-L1) is a transmembrane protein that is expressed on tumor cells that suppresses the T cell-mediated immune response. Therapies targeting the PD-L1 pathway promote anti-tumor immunity and have shown promising results in some types of cancers. However, the functional and therapeutic roles of PD-L...Read More
Krupka Christina Lichtenegger FelixS Khnke Thomas Bgeholz Jan Bcklein Veit Roiss Michael Altmann Torben Do ToUyen Dusek Rachel Wilson Keith Bisht Arnima Terrett Jon Aud Dee Pombo-Villar Esteban Rohlff Christian Hiddemann Wolfgang Subklewe Marion
Oncotarget 2017-04-01
Antibody-based immunotherapy represents a promising strategy to eliminate chemorefractory leukemic cells in acute myeloid leukemia (AML). In this study, we evaluated a novel Fc-engineered antibody against CD157 (MEN1112) for its suitability as immunotherapy in AML. CD157 was expressed in 97% of primary AML patient samples. A sig...Read More
Yu Jihang Alharbi Adel Shan Hongchao Hao Yawei Snetsinger Brooke Rauh MichaelJ Yang Xiaolong
Oncotarget 2017-04-01
Recent studies suggest that lung cancer stem cells (CSCs) may play major roles in lung cancer. Therefore, identification of lung CSC drivers may provide promising targets for lung cancer. TAZ is a transcriptional co-activator and key downstream effector of the Hippo pathway, which plays critical roles in various biological proce...Read More
Panda SudeeptaKumar Boddul SanjayV Jimnez-Andrade GuillerminaYanek Jiang Long Kasza Zsolt Fernandez-Ricaud Luciano Wermeling Fredrik
Genome editing using versions of the bacterial CRISPR/Cas9 system can be used to probe the function of selected genes in any organism. Green Listed is a web-based tool that rapidly designs custom CRISPR screens targeting sets of genes defined by the user. It could thus be used to design screens targeting for example all genes di...Read More
Bradde Serena Vucelja Marija Te?ileanu Tiberiu Balasubramanian Vijay
PLoS computational biology 2017-04-01
The CRISPR (clustered regularly interspaced short palindromic repeats) mechanism allows bacteria to adaptively defend against phages by acquiring short genomic sequences (spacers) that target specific sequences in the viral genome. We propose a population dynamical model where immunity can be both acquired and lost. The model pr...Read More
Kim So-Jung Habib Omer Kim Jin-Soo Han Hyo-Won Koo SooKyung Kim Jung-Hyun
Stem Cell Research 2017-04-01
Kelch-like ECH-associated protein 1 (keap1) is a cysteine-rich protein that interacts with transcription factor Nrf2 in a redox-sensitive manner, leading to the degradation of Nrf2 (Kim et al., 2014a). Disruption of Keap1 results in the induction of Nrf2-related signaling pathways involving the expression of a set of anti-oxidan...Read More
Kim So-Jung Habib Omer Kim Jin-Soo Han Hyo-Won Koo SooKyung Kim Jung-Hyun
Stem Cell Research 2017-04-01
Nuclear factor erythroid 2-related factor 2 (NFE2L2 or Nrf2) is a well-known transcription factor that regulates the expression of a large number of anti-oxidant genes in mammalian cells (J.H. Kim et al., 2014). Here, we generated a homozygous Nrf2 knockout human embryonic stem cell (hESC) line, H9Nrf2KO-A13, using the CRISPR/Ca...Read More
Guk Kyeonghye Keem JooOak Hwang SeulGee Kim Hyeran Kang Taejoon Lim Eun-Kyung Jung Juyeon
Biosensors and Bioelectronics 2017-04-01
Rapid and reliable diagnosis of methicillin-resistant Staphylococcus aureus (MRSA) is crucial for guiding effective patient treatment and preventing the spread of MRSA infections. Nonetheless, further simplification of MRSA detection procedures to shorten detection time and reduce labor relative to that of conventional methods r...Read More
Xiang Guanghai Zhang Xingying An Chenrui Cheng Chen Wang Haoyi
Journal of Genetics and Genomics 2017-04-01
Zinc-finger nuclease (ZFN), transcription activator-like effector nuclease (TALEN), and clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9 (CRISPR-Cas9) are the most commonly used genome editing tools. Previous studies demonstrated that hypothermia treatment increased the mutation rates induced...Read More
Guo Huidong Chu Yajing Wang Le Chen Xing Chen Yangpeng Cheng Hui Zhang Lei Zhou Yuan Yang Feng-Chun Cheng Tao Xu Mingjiang Zhang Xiaobing Zhou Jianfeng Yuan Weiping
International Journal of Cancer 2017-04-01
Interaction of HOXA9/MEIS1/PBX3 is responsible for hematopoietic system transformation in MLL-rearranged (MLL-r) leukemia. Of these genes, HOXA9 has been shown to be critical for leukemia cell survival, while MEIS1 has been identified as an essential regulator for leukemia stem cell (LSC) maintenance. Although significantly high...Read More
Zhao Changlong Shu Xueqin Sun Baolin
Applied and Environmental Microbiology 2017-04-01
There has been constant absence of an efficient gene knockdown method in the important human pathogen Staphylococcus aureus like RNA interference in eukaryotes. The early developed antisense RNA technology is mainly applied for forward genetic screen, but is rather limiting in specific gene knockdown for the lack of rational ant...Read More
DeWitt MarkA Corn JacobE Carroll Dana
Methods 2017-04-01
The CRISPR-Cas genome editing system is very powerful. The format of the CRISPR reagents and the means of delivery are often important factors in targeting efficiency. Delivery of recombinant Cas9 protein and guide RNA (gRNA) as a preformed ribonucleoprotein (RNP) complex has recently emerged as a powerful and general approach t...Read More
Gootenberg JonathanS Abudayyeh OmarO Lee JeongWook Essletzbichler Patrick Dy AaronJ Joung Julia Verdine Vanessa Donghia Nina Daringer NicholeM Freije CatherineA Myhrvold Cameron Bhattacharyya RobyP Livny Jonathan Regev Aviv Koonin EugeneV Hung DeborahT Sabeti PardisC Collins JamesJ Zhang Feng
Science 2017-04-01
Rapid, inexpensive, and sensitive nucleic acid detection may aid point-of-care pathogen detection, genotyping, and disease monitoring. The RNA-guided, RNA-targeting clustered regularly interspaced short palindromic repeats (CRISPR) effector Cas13a (previously known as C2c2) exhibits a collateral effect of promiscuous ribonucleas...Read More
Qazi MA Vora P Venugopal C Sidhu SS Moffat J Swanton C Singh SK
Annals of Oncology 2017-04-01
Intratumoral heterogeneity (ITH) has increasingly being described for multiple cancers as the root cause of therapy resistance. Recent studies have started to explore the scope of ITH in glioblastoma (GBM), a highly aggressive and fatal form of brain tumor, to explain its inevitable therapy resistance and disease relapse. In thi...Read More
Szafranski Przemyslaw Karolak JustynaA Lanza Denise Gaj?cka Marzena Heaney Jason Stankiewicz Pawe?
Mammalian Genome 2017-04-01
Recent genome editing techniques, including CRISPR mutagenesis screens, offer unparalleled opportunities to study the regulatory non-coding genomic regions, enhancers, promoters, and functional non-coding RNAs. Heterozygous point mutations in FOXF1 and genomic deletion copy-number variants at chromosomal region 16q24.1 involving...Read More
Chandrasekaran ArunPandian Song Minjung Ramakrishna Suresh
Experientia. Basel 2017-04-01
Human pluripotent stem cells comprise induced pluripotent and embryonic stem cells, which have tremendous potential for biological and therapeutic applications. The development of efficient technologies for the targeted genome alteration of stem cells in disease models is a prerequisite for utilizing stem cells to their full pot...Read More
Fan Huahao Qiao Luhua Kang Kyung-Don Fan Junfen Wei Wensheng Luo Guangxiang
Journal of Virology 2017-04-01
Hepatitis C virus (HCV) infection requires multiple receptors for its attachment and entry to the cell. Our previous studies found that human syndecan-1 (SDC-1), SDC-2, and T cell immunoglobulin and mucin domain-containing protein 1 (TIM-1) are HCV attachment receptors. Other cell surface molecules such as CD81, CLDN1, OCLN, SR-...Read More
Chirieleison StevenM Marsh RebeccaA Kumar Prathna Rathkey JosephK Dubyak GeorgeR Abbott DerekW
Journal of Biological Chemistry 2017-04-01
The X-linked inhibitor of apoptosis (XIAP) protein has been identified as a key genetic driver of two distinct inflammatory disorders, X-linked lymphoproliferative syndrome 2 (XLP-2) and very early onset inflammatory bowel disease (VEO-IBD). Molecularly, the role of XIAP mutations in the pathogenesis of these disorders is unclea...Read More
Lo Chiu-An Greben AlexanderW Chen BrianEdwin
BioTechniques 2017-04-01
Cell lines expressing foreign genes have been widely used to produce a variety of recombinant proteins. However, generating recombinant protein-expressing cell lines is usually a lengthy process and the resulting protein expression levels are often inconsistent. Here, we describe an efficient method for making stable cell lines ...Read More
Kichev Anton Eede Pascale Gressens Pierre Thornton Claire Hagberg Henrik
Developmental Neuroscience 2017-04-01
Inflammation in the perinatal brain caused by maternal or intrauterine fetal infection is now well established as an important contributor to the development of perinatal brain injury. Exposure to inflammatory products can impair perinatal brain development and act as a risk factor for neurological dysfunction, cognitive disorde...Read More
Yan Feng Ying Le Li Xiaofang Qiao Bin Meng Qiaohong Yu Liang Yuan Xiangliang Ren Shu-Ting Chan DavidW Shi Liyun Ni Peihua Wang Xuefeng Xu Dakang Hu Yiqun
Oncotarget 2017-04-01
The identification of novel biomarkers of cancer is important for improved diagnosis and prognosis. With an abundant amount of resources in the publicly available database, such as the Cancer Genome Atlas (TCGA) database, an integrative strategy is used to systematically characterize the aberrant patterns of colorectal cancer (C...Read More
Platt RandallJ Zhou Yang Slaymaker IanM Shetty AshwinS Weisbach NielsR Kim Jin-Ah Sharma Jitendra Desai Mitul Sood Sabina Kempton HannahR Crabtree GeraldR Feng Guoping Zhang Feng
Cell Reports 2017-04-01
Autism spectrum disorder (ASD) is a heterogeneous disease, but genetically defined models can provide an entry point to studying the molecular underpinnings of this disorder. We generated germline mutant mice with loss-of-function mutations in Chd8, a denovo mutation strongly associated with ASD, and demonstrate that these mice ...Read More
Saarimki-Vire Jonna Balboa Diego Russell MarkA Saarikettu Juha Kinnunen Matias Keskitalo Salla Malhi Amrinder Valensisi Cristina Andrus Colin Eurola Solja Grym Heli Ustinov Jarkko Wartiovaara Kirmo Hawkins RDavid Silvennoinen Olli Varjosalo Markku Morgan NoelG Otonkoski Timo
Cell Reports 2017-04-01
Activating germline mutations in STAT3 were recently identified as a cause of neonatal diabetes mellitus associated with beta-cell autoimmunity. We have investigated the effect of an activating mutation, STAT3(K392R), on pancreatic development using induced pluripotent stem cells (iPSCs) derived from a patient with neonatal diab...Read More
Wang Lixia Yi Fei Fu Lina Yang Jiping Wang Si Wang Zhaoxia Suzuki Keiichiro Sun Liang Xu Xiuling Yu Yang Qiao Jie Belmonte JuanCarlosIzpisua Yang Ze Yuan Yun Qu Jing Liu Guang-Hui
Protein & Cell 2017-04-01
Amyotrophic lateral sclerosis (ALS) is a complex neurodegenerative disease with cellular and molecular mechanisms yet to be fully described. Mutations in a number of genes including SOD1 and FUS are associated with familial ALS. Here we report the generation of induced pluripotent stem cells (iPSCs) from fibroblasts of familial ...Read More
Perez-Mockus Gantas Roca Vanessa Mazouni Khalil Schweisguth Franois
Journal of Cell Biology 2017-04-01
Crumbs (Crb) is a conserved determinant of apical membrane identity that regulates epithelial morphogenesis in many developmental contexts. In this study, we identify the Crb complex protein Stardust (Sdt) as a target of the E3 ubiquitin ligase Neuralized (Neur) in Drosophila melanogaster Neur interacts with and down-regulates s...Read More
Chatterjee Sumantra Ahituv Nadav
Genomics and Human Genetics 2017-04-01
Gene expression changes, the driving forces for cellular diversity in multicellular organisms, are regulated by a diverse set of gene regulatory elements that direct transcription in specific cells. Mutations in these elements, ranging from chromosomal aberrations to single-nucleotide polymorphisms, are a major cause of human di...Read More
Shinmyo Yohei Kawasaki Hiroshi
Current protocols in neuroscience / editorial board, Jacqueline N. Crawley ... [et al.] 2017-04-01
This unit describes a highly efficient and rapid procedure for brain-specific disruption of genes in the developing mouse brain using pX330 plasmids expressing humanized Cas9 and single-guide RNAs (sgRNAs) against target genes. The pX330 plasmids are delivered into the rodent brain using in utero electroporation. Focusing on the...Read More
Sredni SimoneTreiger Suzuki Mario Yang Jian-Ping Topczewski Jacek Bailey AndersW Gokirmak Tufan Gross JeffreyN deAndrade Alexandre Kondo Akihide Piper DavidR Tomita Tadanori
Pediatric Blood & Cancer 2017-04-01
Malignant rhabdoid tumors (MRTs) are deadly embryonal tumors of the infancy. With poor survival and modest response to available therapies, more effective and less toxic treatments are needed. We hypothesized that a systematic screening of the kinome will reveal kinases that drive rhabdoid tumors and can be targeted by specific ...Read More
Lu Huijie Cui Yong Jiang Liwen Ge Wei
Endocrinology 2017-04-01
Estrogens signal through both nuclear and membrane receptors with most reported effects being mediated via the nuclear estrogen receptors (nERs). Although much work has been reported on nERs in the zebrafish, there is a lack of direct genetic evidence for their functional roles and importance in reproduction. To address this iss...Read More
Kim Daesik Lim Kayeong Kim Sang-Tae Yoon Sun-Heui Kim Kyoungmi Ryu Seuk-Min Kim Jin-Soo
Nature Biotechnology 2017-04-01
Cas9-linked deaminases, also called base editors, enable targeted mutation of single nucleotides in eukaryotic genomes. However, their off-target activity is largely unknown. Here we modify digested-genome sequencing (Digenome-seq) to assess the specificity of a programmable deaminase composed of a Cas9 nickase (nCas9) and the d...Read More
Lanning BryanR Vakoc ChristopherR
Nature Biotechnology 2017-04-01
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Nature Biotechnology 2017-04-01
Zhang MingziM Wong FongTian Wang Yajie Luo Shangwen Lim YeeHwee Heng Elena Yeo WanLin Cobb RyanE Enghiad Behnam Ang EeLui Zhao Huimin
Nature Chemical Biology 2017-04-01
Here we report an efficient CRISPR-Cas9 knock-in strategy to activate silent biosynthetic gene clusters (BGCs) in streptomycetes. We applied this one-step strategy to activate multiple BGCs of different classes in five Streptomyces species and triggered the production of unique metabolites, including a novel pentangular type II ...Read More
Cowan PJ Tector AJ
American Journal of Transplantation 2017-04-01
There has been an upsurge of interest in xenotransplantation in recent years. This resurgence can attributed to a combination of factors. First, there has been a dramatic improvement in efficacy in several preclinical models, with maximum xenograft survival times increasing to 950 days for islets, 945 days for hearts, and 310 da...Read More
Liao Jinrong Lin Jinghui Lin Dong Zou Changyan Kurata Jessica Lin Renjang He Zhiyong Su Ying
Scientific reports 2017-04-01
Epidermal growth factor receptor (EGFR) tyrosine kinase inhibitors (TKIs) are standard treatments for advanced non-small-cell lung cancer (NSCLC) patients. However, acquired resistance to EGFR-TKIs is widely detected across the world, and the exact mechanisms have not been fully demonstrated until now. This study aimed to examin...Read More
Wan Xuehua Koster Kent Qian Lishi Desmond Edward Brostrom Richard Hou Shaobin Douglas JamesT
PLoS ONE 2017-04-01
With its airborne transmission and prolonged latency period, Mycobacterium tuberculosis spreads worldwide as one of the most successful bacterial pathogens and continues to kill millions of people every year. M. tuberculosis lineage 1 is inferred to originate ancestrally based on the presence of the 52-bp TbD1 sequence and analy...Read More
Wells KevinD Prather RandallS
Molecular Reproduction and Development 2017-04-01
The ability to directly manipulate the pig genome through genetic engineering has been available to the research community for over three decades. This technology has progressed from the random insertion of foreign DNA, via a variety of techniques (pronuclear microinjection, sperm mediated gene transfer, and integration of mobil...Read More
Yan Yun Zhao Wukui Huang Yikai Tong Huan Xia Yin Jiang Qing Qin Jinzhong
Scientific reports 2017-04-01
The Polycomb repressive complex 1 (PRC1) is essential for fate decisions of embryonic stem (ES) cells. Emerging evidence suggests that six major variants of PRC1 complex, defined by the mutually exclusive presence of Pcgf subunit, regulate distinct biological processes, yet very little is known about the mechanism by which each ...Read More
Shim Gayong Kim Dongyoon Park GyuThae Jin Hyerim Suh Soo-Kyung Oh Yu-Kyoung
Acta Pharmacologica Sinica 2017-04-01
Gene-editing technology is an emerging therapeutic modality for manipulating the eukaryotic genome by using target-sequence-specific engineered nucleases. Because of the exceptional advantages that gene-editing technology offers in facilitating the accurate correction of sequences in a genome, gene editing-based therapy is being...Read More
Yan Sen Tu Zhuchi Li Shihua Li Xiao-Jiang
Progress in Neuro-Psychopharmacology and Biological Psychiatry 2017-04-01
Aging-related brain diseases consist of a number of important neurodegenerative disorders, including Alzheimer's, Parkinson's, and Huntington's diseases, all of which have become more prevalent as the life expectancy of humans is prolonged. Age-dependent brain disorders are associated with both environmental insults and genetic ...Read More
Wu Junjun Zhang Xia Xia Xiudong Dong Mingsheng
Metabolic Engineering 2017-04-01
Medium-chain fatty acids (MCFAs, 6-10 carbons) are valuable precursors to many industrial biofuels and chemicals, recently engineered reversal of the ?-oxidation (r-BOX) cycle has been proposed as a potential platform for efficient synthesis of MCFAs. Previous studies have made many exciting achievements on functionally characte...Read More
Paix Alexandre Folkmann Andrew Seydoux Geraldine
Methods 2017-04-01
The ability to introduce targeted edits in the genome of model organisms is revolutionizing the field of genetics. State-of-the-art methods for precision genome editing use RNA-guided endonucleases to create double-strand breaks (DSBs) and DNA templates containing the edits to repair the DSBs. Following this strategy, we have de...Read More
Avior Yishai Lezmi Elyad Yanuka Dorit Benvenisty Nissim
Stem cell reports 2017-04-01
Human embryonic stem cells (hESCs) provide a platform for studying human development and understanding mechanisms underlying diseases. Retinoblastoma-1 (RB1) is a key regulator of cell cycling, of which biallelic inactivation initiates retinoblastoma, the most common congenital intraocular malignancy. We developed a model to stu...Read More
Gophna Uri Allers Thorsten Marchfelder Anita
Trends in Microbiology 2017-04-01
The majority of archaea encode CRISPR-Cas systems but only a few CRISPR-Cas-based genetic tools have been developed for organisms from this domain. Nayak and Metcalf have harnessed a bacterial Cas9 protein for genome editing in Methanosarcina acetivorans, enabling efficient gene deletion and replacement.Read More
Cao Mingfeng Gao Meirong Lopez-Garcia CarmenLorena Wu Yutong Seetharam ArunSomwarpet Severin AndrewJosef Shao Zengyi
ACS Synthetic Biology 2017-04-01
Many nonconventional yeast species have highly desirable features that are not possessed by model yeasts, despite that significant technology hurdles to effectively manipulate them lay in front. Scheffersomyces stipitis is one of the most important exemplary nonconventional yeasts in biorenewables industry, which has a high nati...Read More
Kelu JeffreyJ Webb SarahE Parrington John Galione Antony Miller AndrewL
Developmental Biology 2017-04-01
We recently demonstrated a critical role for two-pore channel type 2 (TPC2)-mediated Ca(2+) release during the differentiation of slow (skeletal) muscle cells (SMC) in intact zebrafish embryos, via the introduction of a translational-blocking morpholino antisense oligonucleotide (MO). Here, we extend our study and demonstrate th...Read More
Wang Jing-Zhang Wu Peng Shi Zhi-Min Xu Yan-Li Liu Zhi-Jun
Brain and Development 2017-04-01
Mutations in the dystrophin gene (Dmd) result in Duchenne muscular dystrophy (DMD) and Becker muscular dystrophy (BMD), which afflict many newborn boys. In 2016, Brain and Development published several interesting articles on DMD treatment with antisense oligonucleotide, kinase inhibitor, and prednisolone. Even more strikingly, ...Read More
Whitworth KristinM Prather RandallS
Molecular Reproduction and Development 2017-04-01
Porcine Reproductive and Respiratory Syndrome (PRRS) causes severe reproductive failure in sows, as well as transplacental transfer of PRRS virus (PRRSV) to late-gestation fetuses, resulting in abortions, early farrowing, increased number of stillborn piglets, and weak neonatal piglets. PRRSV-infected boars present with anorexia...Read More
El-Sayed AshrafSA Abdel-Ghany SalahE Ali GulShad
Applied Microbiology and Biotechnology 2017-04-01
Filamentous fungi are prolific repertoire of structurally diverse secondary metabolites of remarkable biological activities such as lovastatin and paclitaxel that have been approved by FDA as drugs for hypercholesterolemia and cancer treatment. The clusters of genes encoding lovastatin and paclitaxel are cryptic at standard labo...Read More
Rupp LeviJ Schumann Kathrin Roybal KoleT Gate RachelE Ye ChunJ Lim WendellA Marson Alexander
Scientific reports 2017-04-01
Immunotherapies with chimeric antigen receptor (CAR) T cells and checkpoint inhibitors (including antibodies that antagonize programmed cell death protein 1 [PD-1]) have both opened new avenues for cancer treatment, but the clinical potential of combined disruption of inhibitory checkpoints and CAR T cell therapy remains incompl...Read More
Hahn Friedrich Schmalen Adrian Setz Christian Friedrich Melanie Schler Stefan Klle Julia Spranger Robert Rauch Pia Fraedrich Kirsten Reif Tatjana Karius-Fischer Julia Balasubramanyam Ashok Henklein Petra Fossen Torgils Schubert Ulrich
PLoS ONE 2017-04-01
There is a significantly higher risk for type II diabetes in HIV-1 carriers, albeit the molecular mechanism for this HIV-related pathology remains enigmatic. The 52 amino acid HIV-1 p6 Gag protein is synthesized as the C-terminal part of the Gag polyprotein Pr55. In this context, p6 promotes virus release by its two late (L-) do...Read More
Cornu TatjanaI Mussolino Claudio Cathomen Toni
Nature Medicine 2017-04-01
Recent progress in developing programmable nucleases, such as zinc-finger nucleases, transcription activator-like effector nucleases (TALENs) and clustered regularly interspaced short palindromic repeat (CRISPR)-Cas nucleases, have paved the way for gene editing to enter clinical practice. This translation is a result of combini...Read More
Pashos EvanthiaE Park YoSon Wang Xiao Raghavan Avanthi Yang Wenli Abbey Deepti Peters DerekT Arbelaez Juan Hernandez Mayda Kuperwasser Nicolas Li Wenjun Lian Zhaorui Liu Ying Lv Wenjian Lytle-Gabbin StaceyL Marchadier DawnH Rogov Peter Shi Jianting Slovik KatherineJ Stylianou IoannisM Wang Li Yan Ruilan Zhang Xiaolan Kathiresan Sekar Duncan StephenA Mikkelsen TarjeiS Morrisey EdwardE Rader DanielJ Brown ChristopherD Musunuru Kiran
Cell Stem Cell 2017-04-01
Genome-wide association studies have struggled to identify functional genes and variants underlying complex phenotypes. We recruited a multi-ethnic cohort of healthy volunteers (n= 91) and used their tissue to generate induced pluripotent stem cells (iPSCs) and hepatocyte-like cells (HLCs) for genome-wide mapping of expression q...Read More
Slobodin Boris Han Ruiqi Calderone Vittorio Vrielink JoachimAFOude Loayza-Puch Fabricio Elkon Ran Agami Reuven
Cell 2017-04-01
Transcription and translation are two main pillars of gene expression. Due to the different timings, spots of action, and mechanisms of regulation, these processes are mainly regarded as distinct and generally uncoupled, despite serving a common purpose. Here, we sought for a possible connection between transcription and transla...Read More
Lu Quinn Livi GeorgeP Modha Sundip Yusa Kosuke Macarrn Ricardo Dow DavidJ
Expert Opinion on Drug Discovery 2017-04-01
The analysis of pharmaceutical industry data indicates that the major reason for drug candidates failing in late stage clinical development is lack of efficacy, with a high proportion of these due to erroneous hypotheses about target to disease linkage. More than ever, there is a requirement to better understand potential new dr...Read More
Chen Fuqiang Ding Xiao Feng Yongmei Seebeck Timothy Jiang Yanfang Davis GregoryD
Nature Communications 2017-04-01
Bacterial CRISPR-Cas systems comprise diverse effector endonucleases with different targeting ranges, specificities and enzymatic properties, but many of them are inactive in mammalian cells and are thus precluded from genome-editing applications. Here we show that the type II-B FnCas9 from Francisella novicida possesses novel p...Read More
vandeWiel CCM Schaart JG Lotz LAP Smulders MJM
Plant Biotechnology Reports 2017-04-01
One of the most promising New Plant Breeding Techniques is genome editing (also called gene editing) with the help of a programmable site-directed nuclease (SDN). In this review, we focus on SDN-1, which is the generation of small deletions or insertions (indels) at a precisely defined location in the genome with zinc finger nuc...Read More
Bi Pengpeng Ramirez-Martinez Andres Li Hui Cannavino Jessica McAnally JohnR Shelton JohnM Snchez-Ortiz Efrain Bassel-Duby Rhonda Olson EricN
Science 2017-04-01
Skeletal muscle formation occurs through fusion of myoblasts to form multinucleated myofibers. From a genome-wide clustered regularly interspaced short palindromic repeats (CRISPR) loss-of-function screen for genes required for myoblast fusion and myogenesis, we discovered an 84-amino acid muscle-specific peptide that we call My...Read More
Jackson SimonA McKenzie RebeccaE Fagerlund RobertD Kieper SebastianN Fineran PeterC Brouns StanJJ
Science 2017-04-01
Bacteria and archaea are engaged in a constant arms race to defend against the ever-present threats of viruses and invasion by mobile genetic elements. The most flexible weapons in the prokaryotic defense arsenal are the CRISPR-Cas adaptive immune systems. These systems are capable of selective identification and neutralization ...Read More
Neitemeier Sandra Jelinek Anja Laino Vincenzo Hoffmann Lena Eisenbach Ina Eying Roman Ganjam GouthamK Dolga AmaliaM Oppermann Sina Culmsee Carsten
Redox Biology 2017-04-01
Ferroptosis has been defined as an oxidative and iron-dependent pathway of regulated cell death that is distinct from caspase-dependent apoptosis and established pathways of death receptor-mediated regulated necrosis. While emerging evidence linked features of ferroptosis induced e.g. by erastin-mediated inhibition of the Xc(-) ...Read More
Liu TinaY Iavarone AnthonyT Doudna JenniferA
PLoS ONE 2017-04-01
[This corrects the article DOI: 10.1371/journal.pone.0170552.].Read More
Hwang Yongsung Broxmeyer HalE Lee ManRyul
Current Opinion in Hematology 2017-04-01
Hematopoietic cell transplantation (HCT) is a successful treatment modality for patients with malignant and nonmalignant disorders, usually when no other treatment option is available. The cells supporting long-term reconstitution after HCT are the hematopoietic stem cells (HSCs), which can be limited in numbers. Moreover, findi...Read More
Zhang Xu Schnorrer Frank
FEBS Journal 2017-04-01
Conditional protein depletion is highly desirable for investigating protein functions in complex organisms. In this issue, Bence and colleagues combined auxin-inducible degradation with CRISPR, establishing an elegant tool to control protein levels. They achieve precise spatio-temporal control of protein degradation during Droso...Read More
Li Hao Sheng Chunyu Wang Shan Yang Lang Liang Yuan Huang Yong Liu Hongbo Li Peng Yang Chaojie Yang Xiaoxia Jia Leili Xie Jing Wang Ligui Hao Rongzhang Du Xinying Xu Dongping Zhou Jianjun Li Mingzhen Sun Yansong Tong Yigang Li Qiao Qiu Shaofu Song Hongbin
Frontiers in Cellular and Infection Microbiology 2017-04-01
The presence of hepatitis B virus (HBV) covalently closed circular DNA (cccDNA) and the permanent integration of HBV DNA into the host genome confers the risk of viral reactivation and hepatocellular carcinoma. Nucleoside/nucleotide analogs alone have little or no capacity to eliminate replicative HBV templates consisting of ccc...Read More
Baud Amelie Flint Jonathan
Disease Models & Mechanisms 2017-04-01
Identifying genes and pathways that contribute to differences in neurobehavioural traits is a key goal in psychiatric research. Despite considerable success in identifying quantitative trait loci (QTLs) associated with behaviour in laboratory rodents, pinpointing the causal variants and genes is more challenging. For a long time...Read More
Tao Wei Lv Li Chen Guo-Qiang
Microbial Cell Factories 2017-04-01
Clustered regularly interspaced short palindromic repeats interference (CRISPRi) has provided an efficient approach for targeted gene inhibition. A non-model microorganism Halomonas species TD01 has been developed as a promising industrial producer of polyhydroxyalkanoates (PHA), a family of biodegradable polyesters accumulated ...Read More
Ranjan Alok Srivastava SanjayK
Oncotarget 2017-04-01
Glioblastoma (GBM) is the most common brain tumor with poor survival rate. Our results show that penfluridol, an antipsychotic drug significantly reduced the survival of ten adult and pediatric glioblastoma cell lines with IC50 ranging 2-5 ?M after 72 hours of treatment and induced apoptosis. Penfluridol treatment suppressed the...Read More
Li Ming Gong Luyao Zhao Dahe Zhou Jian Xiang Hua
Nucleic Acids Research 2017-04-01
Prokaryotes memorize invader information by incorporating alien DNA as spacers into CRISPR arrays. Although the spacer size has been suggested to be predefined by the architecture of the acquisition complex, there is usually an unexpected heterogeneity. Here, we explored the causes of this heterogeneity in Haloarcula hispanica I...Read More
Davies FaithCJ Hope JillyE McLachlan Fiona Nunez Francis Doig Jennifer Bengani Hemant Smith Colin Abbott CatherineM
Scientific reports 2017-04-01
De novo heterozygous missense mutations in the gene encoding translation elongation factor eEF1A2 have recently been found to give rise to neurodevelopmental disorders. Children with mutations in this gene have developmental delay, epilepsy, intellectual disability and often autism; the most frequently occurring mutation is G70S...Read More
Hough SorenH Kancleris Kris Brody Leigh Humphryes-Kirilov Neil Wolanski Joseph Dunaway Keith Ajetunmobi Ayokunmi Dillard Victor
BMC Bioinformatics 2017-04-01
Jiang Fuguo Doudna JenniferA
Biophysics 2017-04-01
Many bacterial clustered regularly interspaced short palindromic repeats (CRISPR)-CRISPR-associated (Cas) systems employ the dual RNA-guided DNA endonuclease Cas9 to defend against invading phages and conjugative plasmids by introducing site-specific double-stranded breaks in target DNA. Target recognition strictly requires the ...Read More
Kim SeongKeun Kim Haseong Ahn Woo-Chan Park Kwang-Hyun Woo Eui-Jeon Lee Dae-Hee Lee Seung-Goo
ACS Synthetic Biology 2017-04-01
Clustered regularly interspaced short palindromic repeats interference (CRISPRi) is an emerging technology for artificial gene regulation. Type II CRISPR-Cas endonuclease Cas9 is the most widely used protein for gene regulation with CRISPRi. Here, we present type V-A CRISPR-Cas endonuclease Cpf1-based CRISPRi. We constructed an ...Read More
Fears Robin TerMeulen Volker
eLife 2017-04-01
An EASAC working group on genome editing recommends that regulators should focus on specific applications of these new techniques rather than attempting to regulate genome editing itself as a new technology.Read More
Basu Sambuddha Adams Levi Guhathakurta Subhrangshu Kim Yoon-Seong
Scientific reports 2017-04-01
?-synuclein (?-SYN) is a major pathologic contributor to Parkinson's disease (PD). Multiplication of ?-SYN encoding gene (SNCA) is correlated with early onset of the disease underlining the significance of its transcriptional regulation. Thus, monitoring endogenous transcription of SNCA is of utmost importance to understand PD p...Read More
Kelton William Waindok AnnCathrin Pesch Theresa Pogson Mark Ford Kyle Parola Cristina Reddy SaiT
Scientific reports 2017-04-01
The development of programmable nucleases has enabled the application of new genome engineering strategies for cellular immunotherapy. While targeted nucleases have mostly been used to knock-out or knock-in genes in immune cells, the scarless exchange of entire immunogenomic alleles would be of great interest. In particular, rep...Read More
Wu Dan Guan Xiaoyu Zhu Yuwei Ren Kuan Huang Zhiwei
Cell Research 2017-04-01
Yasui Yukiko Tanaka Wakana Sakamoto Tomoaki Kurata Tetsuya Hirano Hiro-Yuki
Plant and Cell Physiology 2017-04-01
Meristems such as the shoot apical meristem and flower meristem (FM) act as a reservoir of stem cells, which reproduce themselves and supply daughter cells for the differentiation of lateral organs. In Oryza sativa (rice), the FLORAL ORGAN NUMBER2 (FON2) gene, which is similar to Arabidopsis CLAVATA3, is involved in meristem mai...Read More
Kaya Hidetaka Ishibashi Kazuhiro Toki Seiichi
Plant and Cell Physiology 2017-04-01
Split-protein methods-where a protein is split into two inactive fragments that must re-assemble to form an active protein-can be used to regulate the activity of a given protein and reduce the size of gene transcription units. Here, we show that a Staphylococcus aureus Cas9 (SaCas9) can be split, and that split-SaCas9 expressed...Read More
Ma Lai Zhang Dechun Miao Qisong Yang Jing Xuan Yuanhu Hu Yibing
Plant and Cell Physiology 2017-04-01
This study investigated the role of the sugar transporter OsSWEET11 during the early stage of rice caryopsis development using ?-glucoronidase (GUS) to represent its expression, together with clustered regularly interspaced short palindromic repeats-CRISPR-associated protein 9 (CRISPR-Cas9)-mediated knockout, cross-fertilization...Read More
Murovec Jana Pirc ?an Yang Bing
Plant Biotechnology Journal 2017-04-01
CRISPR-mediated genome editing using the Streptococcus pyogenes Cas9 enzyme is revolutionizing life science by providing new, precise, facile and high-throughput tools for genetic modification by the specific targeting of double-strand breaks in the genome of hosts. Plant biotechnologists have extensively used the S.pyogenes Cas...Read More
Peng Aihong Chen Shanchun Lei Tiangang Xu Lanzhen He Yongrui Wu Liu Yao Lixiao Zou Xiuping
Plant Biotechnology Journal 2017-04-01
Citrus canker, caused by Xanthomonas citri subsp. citri (Xcc), is severely damaging to the global citrus industry. Targeted editing of host disease-susceptibility genes represents an interesting and potentially durable alternative in plant breeding for resistance. Here, we report improvement of citrus canker resistance through C...Read More
Zhang Yan
Pathogens and Disease 2017-04-01
Bacteria and archaea possess numerous defense systems to combat viral infections and other mobile genetic elements. Uniquely among these, CRISPR-Cas (clustered, regularly interspaced short palindromic repeats-CRISPR associated) provides adaptive genetic interference against foreign nucleic acids. Here we review recent advances o...Read More
Mendoza-Garca Patricia Hugosson Fredrik Fallah Mahsa Higgins MichaelL Iwasaki Yasuno Pfeifer Kathrin Wolfstetter Georg Varshney Gaurav Popichenko Dmitry Gergen JPeter Hens Korneel Deplancke Bart Palmer RuthH
PLoS Genetics 2017-04-01
The Anaplastic Lymphoma Kinase (Alk) receptor tyrosine kinase (RTK) plays a critical role in the specification of founder cells (FCs) in the Drosophila visceral mesoderm (VM) during embryogenesis. Reporter gene and CRISPR/Cas9 deletion analysis reveals enhancer regions in and upstream of the Alk locus that influence tissue-speci...Read More
Klann TylerS Black JoshuaB Chellappan Malathi Safi Alexias Song Lingyun Hilton IsaacB Crawford GregoryE Reddy TimothyE Gersbach CharlesA
Nature Biotechnology 2017-04-01
Large genome-mapping consortia and thousands of genome-wide association studies have identified non-protein-coding elements in the genome as having a central role in various biological processes. However, decoding the functions of the millions of putative regulatory elements discovered in these studies remains challenging. CRISP...Read More
Schaefer SM Segalada C Cheng PF Bonalli M Parfejevs V Levesque MP Dummer R Nicolis SK Sommer L
Oncogene 2017-04-01
Tumor initiation and metastasis formation in many cancers have been associated with emergence of a gene expression program normally active in embryonic or organ-specific stem cells. In particular, the stem cell transcription factor Sox2 is not only expressed in a variety of tumors, but is also required for their formation. Melan...Read More
Kolli Nivya Lu Ming Maiti Panchanan Rossignol Julien Dunbar GrayL
International Journal of Molecular Sciences 2017-04-01
Huntington's disease (HD) is a fatal neurodegenerative genetic disease characterized by a loss of neurons in the striatum. It is caused by a mutation in the Huntingtin gene (HTT) that codes for the protein huntingtin (HTT). The mutant Huntingtin gene (mHTT) contains extra poly-glutamine (CAG) repeats from which the translated mu...Read More
Chen Jingjing Lai Yiling Wang Lili Zhai Suzhen Zou Gen Zhou Zhihua Cui Chunlai Wang Sibao
Scientific reports 2017-04-01
Beauveria bassiana is an environmentally friendly alternative to chemical insecticides against various agricultural insect pests and vectors of human diseases. However, its application has been limited due to slow kill and sensitivity to abiotic stresses. Understanding of the molecular pathogenesis and physiological characterist...Read More
Yin Chaoran Zhang Ting Qu Xiying Zhang Yonggang Putatunda Raj Xiao Xiao Li Fang Xiao Weidong Zhao Huaqing Dai Shen Qin Xuebin Mo Xianming Young Won-Bin Khalili Kamel Hu Wenhui
Molecular Therapy 2017-04-01
CRISPR-associated protein 9 (Cas9)-mediated genome editing provides a promising cure for HIV-1/AIDS; however, gene delivery efficiency invivo remains an obstacle to overcome. Here, we demonstrate the feasibility and efficiency of excising the HIV-1 provirus in three different animal models using an all-in-one adeno-associated vi...Read More
Bhinge Akshay Namboori SeemaC Zhang Xiaoyu VanDongen AntoniusMJ Stanton LawrenceW
Stem cell reports 2017-04-01
Although mutations in several genes with diverse functions have been known to cause amyotrophic lateral sclerosis (ALS), it is unknown to what extent causal mutations impinge on common pathways that drive motor neuron (MN)-specific neurodegeneration. In this study, we combined induced pluripotent stem cells-based disease modelin...Read More
Jako?i?nas Tadas Jensen MichaelK Keasling JayD
Current Opinion in Biotechnology 2017-04-01
CRISPR/Cas9 (clustered regularly interspaced palindromic repeats and the associated protein Cas9) techniques have made genome engineering and transcriptional reprogramming studies more advanced and cost-effective. For metabolic engineering purposes, the CRISPR-based tools have been applied to single and multiplex pathway modific...Read More
Chen Jiang Du Yinan He Xueyan Huang Xingxu Shi YunS
Scientific reports 2017-04-01
The most powerful way to probe protein function is to characterize the consequence of its deletion. Compared to conventional gene knockout (KO), conditional knockout (cKO) provides an advanced gene targeting strategy with which gene deletion can be performed in a spatially and temporally restricted manner. However, for most spec...Read More
Park Kwang-Hyun An Yan Jung Tae-Yang Baek In-Young Noh Haemin Ahn Woo-Chan Hebert Hans Song Ji-Joon Kim Jeong-Hoon Oh Byung-Ha Woo Eui-Jeon
EMBO Reports 2017-04-01
The CRISPR-Cas system is an adaptive and heritable immune response that destroys invading foreign nucleic acids. The effector complex of the Type III CRISPR-Cas system targets RNA and DNA in a transcription-coupled manner, but the exact mechanism of DNA targeting by this complex remains elusive. In this study, an effector Csm ho...Read More
Miann Joffrey Codner GemmaF Caulder Adam Fell Rachel Hutchison Marie King Ruairidh Stewart MichelleE Wells Sara Teboul Lydia
Methods 2017-04-01
The application of CRISPR/Cas9 technology has revolutionised genetics by greatly enhancing the efficacy of genome editing in the early embryo. Furthermore, the system has enabled the generation of allele types previously incompatible with in vivo mutagenesis. Despite its versatility and ease of implementation, CRISPR/Cas9 editin...Read More
Li Wentao Luo Rui He Qigai vanKuppeveld FrankJM Rottier PeterJM Bosch Berend-Jan
Virus Research 2017-04-01
Porcine epidemic diarrhea virus (PEDV) is an emerging pathogenic coronavirus that causes a significant economic burden to the swine industry. The virus infects the intestinal epithelium and causes villous atrophy, resulting in diarrhea and dehydration. Interaction of the viral spike (S) surface glycoprotein - through its S1 subu...Read More
Li Yize Banerjee Shuvojit Goldstein StephenA Dong Beihua Gaughan Christina Rath Sneha Donovan Jesse Korennykh Alexei Silverman RobertH Weiss SusanR
eLife 2017-03-01
ADAR1 isoforms are adenosine deaminases that edit and destabilize double-stranded RNA reducing its immunostimulatory activities. Mutation of ADAR1 leads to a severe neurodevelopmental and inflammatory disease of children, Aicardi-Goutires syndrome. In mice, Adar1 mutations are embryonic lethal but are rescued by mutation of the ...Read More
Yamayoshi Asako Miyoshi Daisuke Zouzumi Yu-Ki Matsuyama Yohei Ariyoshi Jumpei Shimada Naohiko Murakami Akira Wada Takehiko Maruyama Atsushi
Journal of Physical Chemistry B 2017-03-01
DNA sequences capable of forming triplexes induce DNA double-strand breaks that have attracted attention in genome editing technologies (e.g., CRISPR/Cas9 system, TALEN, and ZFN). Therefore, novel functional tools that stabilize triplex DNA structures must be further investigated to spark renewed interest. In this study, we inve...Read More
Ueta Risa Abe Chihiro Watanabe Takahito Sugano ShigeoS Ishihara Ryosuke Ezura Hiroshi Osakabe Yuriko Osakabe Keishi
Scientific reports 2017-03-01
Parthenocarpy in horticultural crop plants is an important trait with agricultural value for various industrial purposes as well as direct eating quality. Here, we demonstrate a breeding strategy to generate parthenocarpic tomato plants using the CRISPR/Cas9 system. We optimized the CRISPR/Cas9 system to introduce somatic mutati...Read More
Patterson AdrianG Yevstigneyeva MariyaS Fineran PeterC
Current Opinion in Microbiology 2017-03-01
CRISPR-Cas systems are prokaryotic immune systems that allow defense against invasion by foreign genetic elements. Through the acquisition of genetic memory representing prior exposure, the CRISPR-Cas machinery identifies complementary nucleic acids and mediates their destruction. Since their discovery, CRISPR-Cas research has e...Read More
Badia Roger Pujantell Maria Torres-Torronteras Javier Menndez-Arias Luis Mart Ramn Ruzo Albert Pauls Eduardo Clotet Bonaventura Ballana Ester Est JosA Riveira-Muoz Eva
Antiviral Research 2017-03-01
SAMHD1 is a triphosphohydrolase that restricts HIV-1 by limiting the intracellular dNTP pool required for reverse transcription. Although SAMHD1 is expressed and active/unphosphorylated in most cell lines, its restriction activity is thought to be relevant only in non-cycling cells. However, an in depth evaluation of SAMHD1 func...Read More
Zhao Wenting Wang Zhen Sun Zewei Wang Shuai Wu Mingjie Zheng Liangrong
Molecular Medicine Reports 2017-03-01
Elevated blood glucose levels contribute to a series of complications in patients with diabetes mellitus, including chronic ulcers and accelerated atherosclerosis. Dysregulated endothelial migration induced by high glucose is important in vascular?associated complications. In the present study, cluster of differentiation (CD)97/...Read More
Alam Shahidul Anugraham Merrina Huang Yen-Lin Kohler RetoS Hettich Timm Winkelbach Katharina Grether Yasmin Lpez MnicaNez Khasbiullina Nailia Bovin NicolaiV Schlotterbeck Gtz Jacob Francis
Scientific reports 2017-03-01
The (neo-) lacto series glycosphingolipids (nsGSLs) comprise of glycan epitopes that are present as blood group antigens, act as primary receptors for human pathogens and are also increasingly associated with malignant diseases. Beta-1, 3-N-acetyl-glucosaminyl-transferase 5 (B3GNT5) is suggested as the key glycosyltransferase fo...Read More
Hirschhorn Tal Levi-Hofman Michal Danziger Oded Smorodinsky NechamaI Ehrlich Marcelo
Experientia. Basel 2017-03-01
The Type-I bone morphogenetic protein receptors (BMPRs), BMPR1A and BMPR1B, present the highest sequence homology among BMPRs, suggestive of functional similitude. However, sequence elements within their extracellular domain, such as signal sequence or N-glycosylation motifs, may result in differential regulation of biosynthetic...Read More
Filipescu Dan Naughtin Monica Podsypanina Katrina Lejour Vincent Wilson Laurence Gurard-Levin ZacharyA Orsi GuillermoA Simeonova Iva Toufektchan Eleonore Attardi LauraD Toledo Franck Almouzni Genevive
Genes & Development 2017-03-01
In mammals, centromere definition involves the histone variant CENP-A (centromere protein A), deposited by its chaperone, HJURP (Holliday junction recognition protein). Alterations in this process impair chromosome segregation and genome stability, which are also compromised by p53 inactivation in cancer. Here we found that CENP...Read More
Guo Yuxuan VanDusen NathanJ Zhang Lina Gu Weiliang Sethi Isha Guatimosim Silvia Ma Qing Jardin BlakeD Ai Yulan Zhang Donghui Chen Biyi Guo Ang Yuan Guo-Cheng Song Long-Sheng Pu WilliamT
Circulation Research 2017-03-01
Rationale: Loss-of-function studies in cardiac myocytes (CMs) are currently limited by the need for appropriate conditional knockout alleles. The factors that regulate CM maturation are poorly understood. Prior studies on CM maturation have been confounded by heart dysfunction caused by whole organ gene inactivation. Objective: ...Read More
Kan Yinan Ruis Brian Takasugi Taylor Hendrickson EricAbner
Genome Research 2017-03-01
The use of programmable meganucleases is transforming genome editing and functional genomics. CRISPR/Cas9 was developed such that targeted genomic lesions could be introduced in vivo with unprecedented ease. In the presence of homology donors, these lesions facilitate high-efficiency precise genome editing (PGE) via homology-dir...Read More
Guan Juan Liu Harrison Shi Xiaoyu Feng Siyu Huang Bo
Biophysical Journal 2017-03-01
Live imaging of genome has offered important insights into the dynamics of the genome organization and gene expression. The demand to image simultaneously multiple genomic loci has prompted a flurry of exciting advances in multicolor CRISPR imaging, although color-based multiplexing is limited by the need for spectrally distinct...Read More
Cot AllisonJ Raj Arjun
Biophysical Journal 2017-03-01
Modell JoshuaW Jiang Wenyan Marraffini LucianoA
Nature 2017-03-01
Clustered regularly interspaced short palindromic repeats (CRISPR)-Cas systems provide protection against viral and plasmid infection by capturing short DNA sequences from these invaders and integrating them into the CRISPR locus of the prokaryotic host. These sequences, known as spacers, are transcribed into short CRISPR RNA gu...Read More
Nekrasov Vladimir Wang Congmao Win Joe Lanz Christa Weigel Detlef Kamoun Sophien
Scientific reports 2017-03-01
Genome editing has emerged as a technology with a potential to revolutionize plant breeding. In this study, we report on generating, in less than ten months, Tomelo, a non-transgenic tomato variety resistant to the powdery mildew fungal pathogen using the CRISPR/Cas9 technology. We used whole-genome sequencing to show that Tomel...Read More
Yamauchi Takaki Yoshioka Miki Fukazawa Aya Mori Hitoshi Nishizawa NaokoK Tsutsumi Nobuhiro Yoshioka Hirofumi Nakazono Mikio
Plant Cell 2017-03-01
Reactive oxygen species (ROS) produced by the NADPH oxidase, respiratory burst oxidase homolog (RBOH), trigger signal transduction in diverse biological processes in plants. However, the functions of RBOH homologs in rice (Oryza sativa) and other gramineous plants are poorly understood. Ethylene induces the formation of lysigeno...Read More
Leisegang MatthiasS Fork Christian Josipovic Ivana Richter Florian Preussner Jens Hu Jiong Miller MatthewJ Epah JeremyN Hofmann Patrick Gnther Stefan Moll Franziska Valasarajan Chanil Heidler Juliana Ponomareva Yuliya Freiman ThomasM Maegdefessel Lars Plate KarlH Mittelbronn Michel Uchida Shizuka Knne Carsten Stellos Konstantinos Schermuly RalphT Weissmann Norbert Devraj Kavi Wittig Ilka Boon ReinierA Dimmeler Stefanie Pullamsetti SoniS Looso Mario Miller FrancisJ Brandes RalfP
Circulation 2017-03-01
Background -The angiogenic function of endothelial cells is regulated by numerous mechanisms but the impact of long noncoding RNAs (lncRNAs) has hardly been studied. We set out to identify novel and functionally important endothelial lncRNAs. Methods -Epigenetically controlled lncRNAs in human umbilical vein endothelial cells (H...Read More
Jacobi AshleyM Rettig GarrettR Turk Rolf Collingwood MichaelA Zeiner SarahA Quadros RolenM Harms DonaldW Bonthuis PaulJ Gregg Christopher Ohtsuka Masato Gurumurthy ChannabasavaiahB Behlke MarkA
Methods 2017-03-01
Genome editing using the CRISPR/Cas9 system requires the presence of guide RNAs bound to the Cas9 endonuclease as a ribonucleoprotein (RNP) complex in cells, which cleaves the host cell genome at sites specified by the guide RNAs. New genetic material may be introduced during repair of the double-stranded break via homology depe...Read More
Anelli Viviana Villefranc JacquesA Chhangawala Sagar Martinez-McFaline Raul Riva Eleonora Nguyen Anvy Verma Akanksha Bareja Rohan Chen Zhengming Scognamiglio Theresa Elemento Olivier Houvras Yariv
eLife 2017-03-01
Thyroid cancer is common, yet the sequence of alterations that promote tumor formation are incompletely understood. Here, we describe a novel model of thyroid carcinoma in zebrafish that reveals temporal changes due to BRAF(V600E). Through the use of real-time in vivo imaging, we observe disruption in thyroid follicle structure ...Read More
Gayle Sophia Landrette Sean Beeharry Neil Conrad Chris Hernandez Marylens Beckett Paul Ferguson ShawnM Xu Tian Rothberg Jonathan Lichenstein Henri
Autophagy 2017-03-01
We identified the PIKFYVE inhibitor apilimod as a potent and selective cytotoxic agent against B-cell non-Hodgkin lymphoma (B-NHL). Our data robustly establish PIKFYVE as the target through which apilimod kills B-NHL cells and show that apilimod-induced death in B-NHL is mediated by broad disruption of lysosome homeostasis chara...Read More
Yin Xiaojia Biswal AkshayaK Dionora Jacqueline Perdigon KristelM Balahadia ChristianP Mazumdar Shamik Chater Caspar Lin Hsiang-Chun Coe RobertA Kretzschmar Tobias Gray JulieE Quick PaulW Bandyopadhyay Anindya
Plant Cell Reports 2017-03-01
CRISPR-Cas9/Cpf1 system with its unique gene targeting efficiency, could be an important tool for functional study of early developmental genes through the generation of successful knockout plants. The introduction and utilization of systems biology approaches have identified several genes that are involved in early development ...Read More
Shen Lan Hua Yufeng Fu Yaping Li Jian Liu Qing Jiao Xiaozhen Xin Gaowei Wang Junjie Wang Xingchun Yan Changjie Wang Kejian
SCIENCE CHINA Life Science 2017-03-01
The clustered regularly interspaced short palindromic repeats (CRISPR)-associated endonuclease 9 (CRISPR/Cas9) system has emerged as a promising technology for specific genome editing in many species. Here we constructed one vector targeting eight agronomic genes in rice using the CRISPR/Cas9 multiplex genome editing system. By ...Read More
Vervoort Yannick Linares AliciaGutirrez Roncoroni Miguel Liu Chengxun Steensels Jan Verstrepen KevinJ
Current Opinion in Biotechnology 2017-03-01
Genetic engineering and screening of large number of cells or populations is a crucial bottleneck in today's systems biology and applied (micro)biology. Instead of using standard methods in bottles, flasks or 96-well plates, scientists are increasingly relying on high-throughput strategies that miniaturize their experiments to t...Read More
Weinberg BenjaminH Pham NTHang Caraballo LeidyD Lozanoski Thomas Engel Adrien Bhatia Swapnil Wong WilsonW
Nature Biotechnology 2017-03-01
Engineered genetic circuits for mammalian cells often require extensive fine-tuning to perform as intended. We present a robust, general, scalable system, called 'Boolean logic and arithmetic through DNA excision' (BLADE), to engineer genetic circuits with multiple inputs and outputs in mammalian cells with minimal optimization....Read More
Shimatani Zenpei Kashojiya Sachiko Takayama Mariko Terada Rie Arazoe Takayuki Ishii Hisaki Teramura Hiroshi Yamamoto Tsuyoshi Komatsu Hiroki Miura Kenji Ezura Hiroshi Nishida Keiji Ariizumi Tohru Kondo Akihiko
Nature Biotechnology 2017-03-01
We applied a fusion of CRISPR-Cas9 and activation-induced cytidine deaminase (Target-AID) for point mutagenesis at genomic regions specified by single guide RNAs (sgRNAs) in two crop plants. In rice, we induced multiple herbicide-resistance point mutations by multiplexed editing using herbicide selection, while in tomato we gene...Read More
Karvelis Tautvydas Gasiunas Giedrius Siksnys Virginijus
Methods 2017-03-01
Recently the Cas9, an RNA guided DNA endonuclease, emerged as a powerful tool for targeted genome manipulations. Cas9 protein can be reprogrammed to cleave, bind or nick any DNA target by simply changing crRNA sequence, however a short nucleotide sequence, termed PAM, is required to initiate crRNA hybridization to the DNA target...Read More
Lai FrankPui-Ling Lau Sin-Ting Wong JohnKwong-Leong Gui Hongsheng Wang ReesonXu Zhou Tingwen Lai WingHon Tse Hung-Fat Tam PaulKwong-Hang Garcia-Barcelo Maria-Mercedes Ngan EllySau-Wai
Gastroenterology 2017-03-01
Hirschsprung disease is caused by failure of enteric neural crest cells (ENCCs) to fully colonize the bowel, leading to bowel obstruction and megacolon. Heterozygous mutations in the coding region of the RET gene cause a severe form of Hirschsprung disease (total colonic aganglionosis). However, 80% of HSCR patients have short-s...Read More
Kania El?bieta Paj?k Beata O'Prey Jim SierraGonzalez Pablo Litwiniuk Anna Urba?ska Kaja Ryan KevinM Orzechowski Arkadiusz
FEBS Journal 2017-03-01
Verapamil, an L-type calcium channel blocker, has been used successfully to treat cardiovascular diseases. Interestingly, we have recently shown that treatment of cancer cells with verapamil causes an effect on autophagy. As autophagy is known to modulate chemotherapy responses, this prompted us to explore the impact of verapami...Read More
Lopez-Guerrero AidaM Tomas-Martin Patricia Pascual-Caro Carlos Macartney Thomas Rojas-Fernandez Alejandro Ball Graeme Alessi DarioR Pozo-Guisado Eulalia Martin-Romero FranciscoJavier
Scientific reports 2017-03-01
Cell motility and migration requires the reorganization of the cortical cytoskeleton at the leading edge of cells and extracellular Ca(2+) entry is essential for this reorganization. However the molecular nature of the regulators of this pathway is unknown. This work contributes to understanding the role of STIM1 and ORAI1 in th...Read More
Fu Rui Ding Yan Luo Jie Yu Li Li ChengLin Li DongSheng Guo ShiWenWen
Bioscience Reports 2017-03-01
DNA methylation and demethylation play a critical role in the regulation of the molecular pathogenesis of gliomas. TET1 catalyzes the sequential oxidation of 5-methylcytosine to 5-hydroxymethyl-cytosine, leading to eventual DNA demethylation. It has reported that TET1 is a tumor suppressor in several cancers. However, whether TE...Read More
Gandhi Shashank Haeussler Maximilian Razy-Krajka Florian Christiaen Lionel Stolfi Alberto
Developmental Biology 2017-03-01
The CRISPR/Cas9 system has emerged as an important tool for various genome engineering applications. A current obstacle to high throughput applications of CRISPR/Cas9 is the imprecise prediction of highly active single guide RNAs (sgRNAs). We previously implemented the CRISPR/Cas9 system to induce tissue-specific mutations in th...Read More
Chowdhury Saikat Carter Joshua Rollins MaryClareF Golden SarahM Jackson RyanN Hoffmann Connor Nosaka Lyn'Al Bondy-Denomy Joseph Maxwell KarenL Davidson AlanR Fischer ElizabethR Lander GabrielC Wiedenheft Blake
Cell 2017-03-01
Genetic conflict between viruses and their hosts drives evolution and genetic innovation. Prokaryotes evolved CRISPR-mediated adaptive immune systems for protection from viral infection, and viruses have evolved diverse anti-CRISPR (Acr) proteins that subvert these immune systems. The adaptive immune system in Pseudomonas aerugi...Read More
Li Yuchi Li Cailing Lin Shouren Yang Bo Huang Weiren Wu Hanwei Chen Yuanbin Yang Lihua Luo Manling Guo Huan Chen Jianbo Wang Tiantian Ma Qian Gu Yanli Mou Lisha Jiang Zhimao Xia Jun Gui Yaoting
Biology of Reproduction 2017-03-01
Phenotype-driven mutagenesis is an unbiased method to identify novel genes involved in spermatogenesis and other reproductive processes. Male repro29/repro29 mice generated by the Reproductive Genomics Program at the Jackson Laboratory were infertile with deformed sperm and poor motility. Using selected exonic capture and massiv...Read More
Lin Ann Giuliano ChristopherJ Sayles NicoleM Sheltzer JasonM
eLife 2017-03-01
The Maternal Embryonic Leucine Zipper Kinase (MELK) has been reported to be a genetic dependency in several cancer types. MELK RNAi and small-molecule inhibitors of MELK block the proliferation of various cancer cell lines, and MELK knockdown has been described as particularly effective against the highly-aggressive basal/triple...Read More
Yin Hao Kauffman KevinJ Anderson DanielG
Nature Reviews: Drug Discovery 2017-03-01
With the recent development of CRISPR technology, it is becoming increasingly easy to engineer the genome. Genome-editing systems based on CRISPR, as well as transcription activator-like effector nucleases (TALENs) and zinc-finger nucleases (ZFNs), are becoming valuable tools for biomedical research, drug discovery and developme...Read More
Awwad SamahW Abu-Zhayia EnasR Guttmann-Raviv Noga Ayoub Nabieh
EMBO Reports 2017-03-01
Double-strand breaks (DSBs) trigger rapid and transient transcription pause to prevent collisions between repair and transcription machineries at damage sites. Little is known about the mechanisms that ensure transcriptional block after DNA damage. Here, we reveal a novel role of the negative elongation factor NELF in blocking t...Read More
Contreras JorgeL Sherkow JacobS
Science 2017-03-01
Budak Gungor Srivastava Rajneesh Janga SarathChandra
RNA 2017-03-01
RNA-binding proteins (RBPs) control the regulation of gene expression in eukaryotic genomes at post-transcriptional level by binding to their cognate RNAs. Although several variants of CLIP (crosslinking and immunoprecipitation) protocols are currently available to study the global protein-RNA interaction landscape at single-nuc...Read More
Yamamoto Yuta Makiyama Takeru Harita Takeshi Sasaki Kenichi Wuriyanghai Yimin Hayano Mamoru Nishiuchi Suguru Kohjitani Hirohiko Hirose Sayako Chen Jiarong Yokoi Fumika Ishikawa Taisuke Ohno Seiko Chonabayashi Kazuhisa Motomura Hideki Yoshida Yoshinori Horie Minoru Makita Naomasa Kimura Takeshi
Human Molecular Genetics 2017-03-01
Calmodulin is a ubiquitous Ca2+ sensor molecule encoded by three distinct calmodulin genes, CALM1-3. Recently, mutations in CALM1-3 have been reported to be associated with severe early-onset long-QT syndrome (LQTS). However, the underlying mechanism through which heterozygous calmodulin mutations lead to severe LQTS remains unk...Read More
Boehm Erik Zaganelli Sofia Maundrell Kinsey Jourdain AlexisA Thore Stphane Martinou Jean-Claude
Nucleic Acids Research 2017-03-01
FASTK family proteins have been identified as regulators of mitochondrial RNA homeostasis linked to mitochondrial diseases, but much remains unknown about these proteins. We show that CRISPR-mediated disruption of FASTKD1 increases ND3 mRNA level, while disruption of FASTKD4 reduces the level of ND3 and of other mature mRNAs inc...Read More
Lee HyeKyung Willi Michaela Wang Chaochen Yang ChulMin Smith HaroldE Liu Chengyu Hennighausen Lothar
Nucleic Acids Research 2017-03-01
The zinc finger protein CTCF has been invoked in establishing boundaries between genes, thereby controlling spatial and temporal enhancer activities. However, there is limited genetic evidence to support the concept that these boundaries restrict the search space of enhancers. We have addressed this question in the casein locus ...Read More
McMillan BrianJ Zimmerman Brandon Egan EmilyD Lofgren Michael Xu Xiang Hesser Anthony Blacklow StephenC
Glycobiology 2017-03-01
Protein O-fucosyltransferase-1(POFUT1), which transfers fucose residues to acceptor sites on serine and threonine residues of epidermal growth factor-like repeats of recipient proteins, is essential for Notch signal transduction in mammals. Here, we examine the consequences of POFUT1 loss on the oncogenic signaling associated wi...Read More
Read Abigail Gao Shaojian Batchelor Eric Luo Ji
Nucleic Acids Research 2017-03-01
CRISPR/Cas9-based gene knockout libraries have emerged as a powerful tool for functional screens. We present here a set of pre-designed human and mouse sgRNA sequences that are optimized for both high on-target potency and low off-target effect. To maximize the chance of target gene inactivation, sgRNAs were curated to target bo...Read More
Stachler Aris-Edda Turgeman-Grott Israela Shtifman-Segal Ella Allers Thorsten Marchfelder Anita Gophna Uri
Nucleic Acids Research 2017-03-01
CRISPR-Cas systems allow bacteria and archaea to acquire sequence-specific immunity against selfish genetic elements such as viruses and plasmids, by specific degradation of invader DNA or RNA. However, this involves the risk of autoimmunity if immune memory against host DNA is mistakenly acquired. Such autoimmunity has been sho...Read More
Takayama Kazuo Igai Keisuke Hagihara Yasuko Hashimoto Rina Hanawa Morifumi Sakuma Tetsushi Tachibana Masashi Sakurai Fuminori Yamamoto Takashi Mizuguchi Hiroyuki
Nucleic Acids Research 2017-03-01
Genome editing research of human ES/iPS cells has been accelerated by clustered regularly interspaced short palindromic repeats/CRISPR-associated 9 (CRISPR/Cas9) and transcription activator-like effector nucleases (TALEN) technologies. However, the efficiency of biallelic genetic engineering in transcriptionally inactive genes i...Read More
Mulvihill JohnJ Capps Benjamin Joly Yann Lysaght Tamra Zwart HubAE Chadwick Ruth
British Medical Bulletin 2017-03-01
The avalanche of commentaries on CRISPR-Cas9 technology, a bacterial immune system modified to recognize any short DNA sequence, cut it out, and insert a new one, has rekindled hopes for gene therapy and other applications and raised criticisms of engineering genes in future generations.Read More
Gawryszewska Iwona Malinowska Katarzyna Kuch Alicja Chrobak-Chmiel Dorota Trokenheim LucjaLaniewska- Hryniewicz Waleria Sadowy Ewa
Pathogens and Disease 2017-03-01
Enterococcus faecalis represents an important factor of hospital-associated infections (HAIs). The knowledge on its evolution from a commensal to an opportunistic pathogen is still limited; thus, we performed a study to characterise distribution of factors that may contribute to this adaptation. Using a collection obtained from ...Read More
Joung Julia Konermann Silvana Gootenberg JonathanS Abudayyeh OmarO Platt RandallJ Brigham MarkD Sanjana NevilleE Zhang Feng
Nature Protocols 2017-03-01
Forward genetic screens are powerful tools for the unbiased discovery and functional characterization of specific genetic elements associated with a phenotype of interest. Recently, the RNA-guided endonuclease Cas9 from the microbial CRISPR (clustered regularly interspaced short palindromic repeats) immune system has been adapte...Read More
Sieriebriennikov Bogdan Markov GabrielV Witte Hanh Sommer RalfJ
Molecular Biology and Evolution 2017-03-01
Phenotypic plasticity is increasingly recognized to facilitate adaptive change in plants and animals, including insects, nematodes and vertebrates. Plasticity can occur as continuous or discrete (polyphenisms) variation. In social insects, e.g. in ants, some species have workers of distinct size classes while in other closely re...Read More
Hosur Vishnu Low BenjaminE Avery Cindy Shultz LeonardD Wiles MichaelV
Journal of Cellular Biochemistry 2017-03-01
Mice are the most commonly used model organisms to study human disease. Many genetic human diseases can be recapitulated by modifying the mouse genome allowing the testing of existing and novel therapeutics, including combinatorial therapeutics, without putting humans at risk. Specifically, the development of humanized mice, tha...Read More
Arikawa Kentaro
Journal of Physiology 2017-03-01
Butterflies use colour vision when searching for flowers. Unlike the trichromatic retinas of humans (blue, green and red cones; plus rods) and honeybees (ultraviolet, blue and green photoreceptors), butterfly retinas typically have six or more photoreceptor classes with distinct spectral sensitivities. The eyes of the Japanese y...Read More
Schenk Heiko Mller-Deile Janina Kinast Mark Schiffer Mario
Cell and Tissue Research 2017-03-01
Growing numbers of translational genomics studies are based on the highly efficient and versatile zebrafish (Danio rerio) vertebrate model. The increasing types of zebrafish models have improved our understanding of inherited kidney diseases, since they not only display pathophysiological changes but also give us the opportunity...Read More
Miki Daisuke Zhu Peiying Zhang Wencan Mao Yanfei Feng Zhengyan Huang Huan Zhang Hui Li Yanqiang Liu Renyi Zhang Huiming Qi Yijun Zhu Jian-Kang
Scientific reports 2017-03-01
It has been reported that double-stranded break (DSB)-induced small RNAs (diRNAs) are generated via the RNA-directed DNA methylation pathway and function in DSB repair in Arabidposis. However, important questions remain regarding the biogenesis and function of diRNAs. Here, we used CRISPR/Cas9- or TALEN-triggered DSBs to charact...Read More
Liao Liqiu Song Mei Li Xin Tang Lili Zhang Tuo Zhang Lixing Pan Yihang Chouchane Lotfi Ma Xiaojing
Cancer Research 2017-03-01
Patients with triple-negative breast cancers (TNBC) are at high risk for recurrence and metastasis at an early time despite standard treatment, underscoring the need for novel therapeutic modalities. Here, we report for the first time a distinctive and profound role of the E3 ubiquitin ligase UBR5 in the growth and metastasis of...Read More
Shiromani PriyattamJ Peever JohnH
Sleep 2017-03-01
The complexity of the brain is yielding to technology. In the area of sleep neurobiology, conventional neuroscience tools such as lesions, cell recordings, c-Fos, and axon-tracing methodologies have been instrumental in identifying the complex and intermingled populations of sleep- and arousal-promoting neurons that orchestrate ...Read More
Kage Frieda Winterhoff Moritz Dimchev Vanessa Mueller Jan Thalheim Tobias Freise Anika Brhmann Stefan Kollasser Jana Block Jennifer Dimchev Georgi Geyer Matthias Schnittler Hans-Joachim Brakebusch Cord Stradal TheresiaEB Carlier Marie-France Sixt Michael Ks Josef Faix Jan Rottner Klemens
Nature Communications 2017-03-01
Migration frequently involves Rac-mediated protrusion of lamellipodia, formed by Arp2/3 complex-dependent branching thought to be crucial for force generation and stability of these networks. The formins FMNL2 and FMNL3 are Cdc42 effectors targeting to the lamellipodium tip and shown here to nucleate and elongate actin filaments...Read More
Vanoli Fabio Tomishima Mark Feng Weiran Lamribet Khadija Babin Loelia Brunet Erika Jasin Maria
PNAS 2017-03-01
Gene editing techniques have been extensively used to attempt to model recurrent genomic rearrangements found in tumor cells. These methods involve the induction of double-strand breaks at endogenous loci followed by the identification of breakpoint junctions within a population, which typically arise by nonhomologous end joinin...Read More
Li Ruohan Harvey AlanR Hodgetts StuartI Fox ArchaH
RNA 2017-03-01
Large numbers of long noncoding RNAs have been discovered in recent years, but only a few have been characterized. NEAT1 (nuclear paraspeckle assembly transcript 1) is a mammalian long noncoding RNA that is important for the reproductive physiology of mice, cancer development, and the formation of subnuclear bodies termed parasp...Read More
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Cancer Discovery 2017-03-01
Using CRISPR/Cas9 technology, researchers have devised a method to deliver a CAR gene to a specific locus, TRAC, in T cells. This targeted approach yielded therapeutic cells that were more potent even at low doses; in a mouse model of acute lymphoblastic leukemia, they outperformed CAR T cells created with a randomly integrating...Read More
Lemay Marie-Laurence Tremblay DeniseM Moineau Sylvain
ACS Synthetic Biology 2017-03-01
Phages are biological entities found in every ecosystem. Although much has been learned about them in past decades, significant knowledge gaps remain. Manipulating virulent phage genomes is challenging. To date, no efficient gene-editing tools exist for engineering virulent lactococcal phages. Lactococcus lactis is a bacterium e...Read More
Wu Junjun Zhou Peng Zhang Xia Dong Mingsheng
Journal of Industrial Microbiology 2017-03-01
Resveratrol has been the subject of numerous scientific investigations due to its health-promoting activities against a variety of diseases. However, developing feasible and efficient microbial processes remains challenging owing to the requirement of supplementing expensive phenylpropanoic precursors. Here, various metabolic en...Read More
Turgeon Marc-Olivier Silander TanyaL Doycheva Denica Liao Xiao-Hui Rigden Marc Ongaro Luisina Zhou Xiang Joustra SjoerdD Wit JanM Wade MikeG Heuer Heike Refetoff Samuel Bernard DanielJ
Endocrinology 2017-03-01
Loss-of-function mutations in the X-linked immunoglobulin superfamily, member 1 (IGSF1) gene cause central hypothyroidism. IGSF1 is a transmembrane glycoprotein of unknown function expressed in thyrotropin (TSH)-producing thyrotrope cells of the anterior pituitary gland. The protein is cotranslationally cleaved, with only its C-...Read More
Li Lei Jiang Weihong Lu Yinhua
Biotechnology Advances 2017-03-01
With the rapidly growing number of sequenced microbial (meta)genomes, enormous cryptic natural product (NP) biosynthetic gene clusters (BGCs) have been identified, which are regarded as a rich reservoir for novel drug discovery. A series of powerful tools for engineering BGCs has accelerated the discovery and development of phar...Read More
Sekiba Kazuma Yamagami Mari Otsuka Motoyuki Suzuki Tatsunori Kishikawa Takahiro Ishibashi Rei Ohno Motoko Sato Masaya Koike Kazuhiko
Biochemical and Biophysical Research Communications 2017-03-01
Major histocompatibility complex class I polypeptide-related sequence A (MICA) is a prototypical NKG2D ligand. Because immune cells, such as natural killer (NK) cells, recognize virally infected or transformed cells and eliminate them through the interaction between NKG2D receptors on NK cells and NKG2D ligands on pathogenic cel...Read More
PugaYung GisellaL Rieben Robert Bhler Leo Schuurman Henk-Jan Seebach Jrg
Schweizerische medizinische Wochenschrift 2017-03-01
Worldwide, there is a constant rise in the number of patients with end-stage organ failure in critical need for transplants, but the number of organs/cells available from deceased or living human donors is limited. Xenotransplantation using pig organs/tissues repre-sents a potential solution for this shortage; however, it has be...Read More
Park JooYoun Moon BoYoun Park JuwWon Thornton JustinA Park YongHo Seo KeunSeok
Scientific reports 2017-03-01
Discovery of clustered, regularly interspaced, short palindromic repeats and the Cas9 RNA-guided nuclease (CRISPR/Cas9) system provides a new opportunity to create programmable gene-specific antimicrobials that are far less likely to drive resistance than conventional antibiotics. However, the practical therapeutic use of CRISPR...Read More
Wang Ping Mokhtari Ryan Pedrosa Erika Kirschenbaum Michael Bayrak Can Zheng Deyou Lachman HerbertM
Molecular Autism 2017-03-01
CHD8 (chromodomain helicase DNA-binding protein 8), which codes for a member of the CHD family of ATP-dependent chromatin-remodeling factors, is one of the most commonly mutated genes in autism spectrum disorders (ASD) identified in exome-sequencing studies. Loss of function mutations in the gene have also been found in schizoph...Read More
VanHove Bob Guidi Chiara DeWannemaeker Lien Maertens Jo DeMey Marjan
ACS Synthetic Biology 2017-03-01
A problem rarely tackled by current DNA assembly methods is the issue of cloning additional parts into an already assembled construct. Costly PCR workflows are often hindered by repeated sequences, and restriction based strategies impose design constraints for each enzyme used. Here we present Protected Oligonucleotide Duplex As...Read More
Shen JohnPaul Zhao Dongxin Sasik Roman Luebeck Jens Birmingham Amanda Bojorquez-Gomez Ana Licon Katherine Klepper Kristin Pekin Daniel Beckett AlexN Sanchez KyleSalinas Thomas Alex Kuo Chih-Chung Du Dan Roguev Assen Lewis NathanE Chang AaronN Kreisberg JasonF Krogan Nevan Qi Lei Ideker Trey Mali Prashant
Nature Methods 2017-03-01
We developed a systematic approach to map human genetic networks by combinatorial CRISPR-Cas9 perturbations coupled to robust analysis of growth kinetics. We targeted all pairs of 73 cancer genes with dual guide RNAs in three cell lines, comprising 141,912 tests of interaction. Numerous therapeutically relevant interactions were...Read More
Han Kyuho Jeng EdwinE Hess GaelenT Morgens DavidW Li Amy Bassik MichaelC
Nature Biotechnology 2017-03-01
Identification of effective combination therapies is critical to address the emergence of drug-resistant cancers, but direct screening of all possible drug combinations is infeasible. Here we introduce a CRISPR-based double knockout (CDKO) system that improves the efficiency of combinatorial genetic screening using an effective ...Read More
Ta-Shma Asaf Khan TahirN Vivante Asaf Willer JasonR Matak Pavle Jalas Chaim Pode-Shakked Ben Salem Yishay Anikster Yair Hildebrandt Friedhelm Katsanis Nicholas Elpeleg Orly Davis EricaE
The American Journal of Human Genetics 2017-03-01
Despite the accelerated discovery of genes associated with syndromic traits, the majority of families affected by such conditions remain undiagnosed. Here, we employed whole-exome sequencing in two unrelated consanguineous kindreds with central nervous system (CNS), cardiac, renal, and digit abnormalities. We identified homozygo...Read More
Gu Jia Li TongJuan Zhao Lei Liang Xue Fu Xing Wang Jue Shang Zhen Huang Wei Zhou Jianfeng
BioMed Research International 2017-03-01
PAX5 encodes a transcription factor essential for B-cell differentiation, and PAX5 haploinsufficiency is involved in tumorigenesis. There were few studies on how PAX5 haploinsufficiency regulated genes expression to promote tumorigenesis. In this study, we constructed the cell model of PAX5 haploinsufficiency using gene editing ...Read More
Wang Mugui Mao Yanfei Lu Yuming Tao Xiaoping Zhu Jian-Kang
Molecular Plant 2017-03-01
Wang Mugui Lu Yuming Botella JosRamn Mao Yanfei Hua Kai Zhu Jian-Kang
Molecular Plant 2017-03-01
Liu Xuejun Xie Chuanxiao Si Huaijun Yang Jinxiao
Methods 2017-03-01
The increasing burden of the world's population on agriculture necessitates the development of more robust crops. As the amount of information from sequenced crop genomes increases, technology can be used to investigate the function of genes in detail and to design improved crops at the molecular level. Recently, an RNA-programm...Read More
Yamada Mari Watanabe Yuto Gootenberg JonathanS Hirano Hisato Ran FAnn Nakane Takanori Ishitani Ryuichiro Zhang Feng Nishimasu Hiroshi Nureki Osamu
Molecular Cell 2017-03-01
The RNA-guided endonuclease Cas9 generates a double-strand break at DNA target sites complementary to the guide RNA and has been harnessed for the development of a variety of new technologies, such as genome editing. Here, we report the crystal structures of Campylobacter jejuni Cas9 (CjCas9), one of the smallest Cas9 orthologs,...Read More
Lee CiaranM Davis TimothyH Bao Gang
Experimental Physiology 2017-03-01
What is the topic of this review? In this review, we analyse the performance of recently described tools for CRISPR/Cas9 guide RNA design, in particular, design tools that predict CRISPR/Cas9 activity. What advances does it highlight? Recently, many tools designed to predict CRISPR/Cas9 activity have been reported. However, the ...Read More
Chen Yiyu Wang Zhiping Ni Hanwen Xu Yong Chen Qijun Jiang Linjian
SCIENCE CHINA Life Science 2017-03-01
Bassett AndrewR
Mammalian Genome 2017-03-01
The advent of human-induced pluripotent stem cell (hiPSC) technology has provided a unique opportunity to establish cellular models of disease from individual patients, and to study the effects of the underlying genetic aberrations upon multiple different cell types, many of which would not normally be accessible. Combining this...Read More
Shathasivam Premalatha Kollara Alexandra Spybey Thomasina Park Soyeon Clarke Blaise Ringuette MauriceJ Brown TheodoreJ
British Journal of Cancer 2017-03-01
VEPH1 is amplified in several cancers including ovarian but its impact on tumour progression is unknown. Previous work has shown that VEPH1 inhibits TGF? signalling while its Drosophila ortholog increases tissue growth, raising the possibility that VEPH1 could impact tumour growth or progression.Read More
Das Krishna Eisel David Lenkl Clarissa Goyal Ashish Diederichs Sven Dickes Elke Osen Wolfram Eichmller StefanB
PLoS ONE 2017-03-01
In this study, the CRISPR/Cas9 technology was used to establish murine tumor cell lines, devoid of MHC I or MHC II surface expression, respectively. The melanoma cell line B16F10 and the murine breast cancer cell line EO-771, the latter stably expressing the tumor antigen NY-BR-1 (EO-NY), were transfected with an expression plas...Read More
Albadri Shahad DelBene Filippo Revenu Cline
Methods 2017-03-01
With its variety of applications, the CRISPR/Cas9 genome editing technology has been rapidly evolving in the last few years. In the zebrafish community, knock-out reports are constantly increasing but insertion studies have been so far more challenging. With this review, we aim at giving an overview of the homologous directed re...Read More
Mehrotra Rajesh Renganaath Kaushik Kanodia Harsh Loake GaryJ Mehrotra Sandhya
Biotechnology Advances 2017-03-01
Jarrett KelseyE Lee CiaranM Yeh Yi-Hsien Hsu RachelH Gupta Rajat Zhang Min Rodriguez PerlaJ Lee ChangSeok Gillard BaibaK Bissig Karl-Dimiter Pownall HenryJ Martin JamesF Bao Gang Lagor WilliamR
Scientific reports 2017-03-01
Germline manipulation using CRISPR/Cas9 genome editing has dramatically accelerated the generation of new mouse models. Nonetheless, many metabolic disease models still depend upon laborious germline targeting, and are further complicated by the need to avoid developmental phenotypes. We sought to address these experimental limi...Read More
Liu Yaqin Xu Ya Ling Sheng Liu Shasha Yao Jialing
Plant Cell Reports 2017-03-01
Phenotype identification, expression examination, and function prediction declared that the anther-preferential expressing gene PMR may participate in regulation of male gametophyte development in rice. Male germline development in flowering plants produces the pair of sperm cells for double fertilization and the pollen mitosis ...Read More
Jensen EmilD Ferreira Raphael Jako?i?nas Tadas Arsovska Dushica Zhang Jie Ding Ling Smith JustinD David Florian Nielsen Jens Jensen MichaelK Keasling JayD
Microbial Cell Factories 2017-03-01
Transcriptional reprogramming is a fundamental process of living cells in order to adapt to environmental and endogenous cues. In order to allow flexible and timely control over gene expression without the interference of native gene expression machinery, a large number of studies have focused on developing synthetic biology too...Read More
vanTienen LaurensM Mieszczanek Juliusz Fiedler Marc Rutherford TrevorJ Bienz Mariann
eLife 2017-03-01
Wnt/?-catenin signaling elicits context-dependent transcription switches that determine normal development and oncogenesis. These are mediated by the Wnt enhanceosome, a multiprotein complex binding to the Pygo chromatin reader and acting through TCF/LEF-responsive enhancers. Pygo renders this complex Wnt-responsive, by capturin...Read More
Yuan Jingya Chen Sushu Jiao Wu Wang Longfei Wang Limei Ye Wenxue Lu Jie Hong Delin You Siliang Cheng Zhukuan Yang Dong-Lei Chen ZJeffrey
New Phytologist 2017-03-01
Genetic imprinting refers to the unequal expression of paternal and maternal alleles of a gene in sexually reproducing organisms, including mammals and flowering plants. Although many imprinted genes have been identified in plants, the functions of these imprinted genes have remained largely uninvestigated. We report genome-wide...Read More
Hwang Sung-Hee Han Byeal-I Lee Michael
Journal Of Cellular Physiology 2017-03-01
Autophagy can either promote or inhibit cell death in different cellular contexts. In this study, we investigated the role of autophagy in ATG5 knockout (KO) cell line established using CRISPR/Cas9 system. In ATG5 KO cells, RT-PCR and immunoblot of LC3 confirmed the functional gene knockout. We found that knockout of ATG5 signif...Read More
Halim Danny Wilson MichaelP Oliver Daniel Brosens Erwin Verheij JokeBGM Han Yu Nanda Vivek Lyu Qing Doukas Michael Stoop Hans Brouwer RutgerWW vanIJcken WilfredFJ Slivano OrazioJ Burns AlanJ Christie ChristineK deMesyBentley KarenL Brooks AliceS Tibboel Dick Xu Suowen Jin ZhengGen Djuwantono Tono Yan Wei Alves MariaM Hofstra RobertMW Miano JosephM
PNAS 2017-03-01
Megacystis microcolon intestinal hypoperistalsis syndrome (MMIHS) is a congenital visceral myopathy characterized by severe dilation of the urinary bladder and defective intestinal motility. The genetic basis of MMIHS has been ascribed to spontaneous and autosomal dominant mutations in actin gamma 2 (ACTG2), a smooth muscle cont...Read More
Thirant Ccile Ignacimouttou Cathy Lopez CcileK Diop M'Boyba LeMoul Lou Thiollier Clarisse Siret Aurlie Dessen Phillipe Aid Zakia Rivire Julie Rameau Philippe Lefebvre Cline Khaled Mehdi Leverger Guy Ballerini Paola Petit Arnaud Raslova Hana Carmichael CatherineL Kile BenjaminT Soler Eric Crispino JohnD Wichmann Christian Pflumio Franoise Schwaller Jrg Vainchenker William Lobry Camille Droin Nathalie Bernard OlivierA Malinge Sbastien Mercher Thomas
Cancer Cell 2017-03-01
Chimeric transcription factors are a hallmark of human leukemia, but the molecular mechanisms by which they block differentiation and promote aberrant self-renewal remain unclear. Here, we demonstrate that the ETO2-GLIS2 fusion oncoprotein, which is found in aggressive acute megakaryoblastic leukemia, confers megakaryocytic iden...Read More
Yu Wenhan Mookherjee Suddhasil Chaitankar Vijender Hiriyanna Suja Kim Jung-Woong Brooks Matthew Ataeijannati Yasaman Sun Xun Dong Lijin Li Tiansen Swaroop Anand Wu Zhijian
Nature Communications 2017-03-01
In retinitis pigmentosa, loss of cone photoreceptors leads to blindness, and preservation of cone function is a major therapeutic goal. However, cone loss is thought to occur as a secondary event resulting from degeneration of rod photoreceptors. Here we report a genome editing approach in which adeno-associated virus (AAV)-medi...Read More
Li Xiufeng Zhou Wenjia Ren Yuekun Tian Xiaojie Lv Tianxiao Wang Zhenyu Fang Jun Chu Chengcai Yang Jie Bu Qingyun
Journal of Genetics and Genomics 2017-03-01
Luo Lin Tong SamuelJ Wall AdamA Khromykh Tatiana Sweet MatthewJ Stow JenniferL
Immunology and Cell Biology 2017-03-01
Protein tyrosine phosphorylation guides many molecular interactions for cellular functions. SCIMP is a transmembrane adaptor protein (TRAP) family member that mediates selective proinflammatory cytokine responses generated by pathogen-activated Toll-like receptor (TLR) pathways in macrophages. TLR activation triggers SCIMP phosp...Read More
Qin Peiwu Parlak Mahmut Kuscu Cem Bandaria Jigar Mir Mustafa Szlachta Karol Singh Ritambhara Darzacq Xavier Yildiz Ahmet Adli Mazhar
Nature Communications 2017-03-01
Imaging chromatin dynamics is crucial to understand genome organization and its role in transcriptional regulation. Recently, the RNA-guidable feature of CRISPR-Cas9 has been utilized for imaging of chromatin within live cells. However, these methods are mostly applicable to highly repetitive regions, whereas imaging regions wit...Read More
Ryder Peter McHale Marcus Fort Antoine Spillane Charles
Plant Cell Reports 2017-03-01
RNA-guided endonuclease-mediated targeted mutagenesis using the clustered regularly interspersed short palindromic repeats (CRISPR)/Cas9 system has been successful at targeting specific loci for modification in plants. While polyploidy is an evolutionary mechanism enabling plant adaptation, the analysis of gene function in polyp...Read More
Page SC Hamersky GR Gallo RA Rannals MD Calcaterra NE Campbell MN Mayfield B Briley A Phan BN Jaffe AE Maher BJ
Molecular Psychiatry 2017-03-01
Disruption of the laminar and columnar organization of the brain is implicated in several psychiatric disorders. Here, we show in utero gain-of-function of the psychiatric risk gene transcription factor 4 (TCF4) severely disrupts the columnar organization of medial prefrontal cortex (mPFC) in a transcription- and activity-depend...Read More
Yeganeh Meghdad Praz Viviane Cousin Pascal Hernandez Nouria
Genes & Development 2017-03-01
Overlapping gene arrangements can potentially contribute to gene expression regulation. A mammalian interspersed repeat (MIR) nested in antisense orientation within the first intron of the Polr3e gene, encoding an RNA polymerase III (Pol III) subunit, is conserved in mammals and highly occupied by Pol III. Using a fluorescence a...Read More
Chen Jing Crutchley John Zhang Dadong Owzar Kouros Kastan MichaelB
Cancer Discovery 2017-03-01
Cellular responses to DNA damage are critical determinants of cancer development and aging-associated pathogenesis. Here we report a novel DNA damage response pathway that regulates alternative splicing of numerous gene products, including the human tumor suppressor p53, and controls DNA damage-induced cellular senescence. In br...Read More
Canver MatthewC Bauer DanielE Orkin StuartH
Methods 2017-03-01
Methodologies to interrogate non-coding regions have lagged behind coding regions despite comprising the vast majority of the genome. However, the rapid evolution of clustered regularly interspaced short palindromic repeats (CRISPR)-based genome editing has provided a multitude of novel techniques for laboratory investigation in...Read More
Guernet Alexis Grumolato Luca
Methods 2017-03-01
The CRISPR/Cas9 revolution has democratized access to genome editing in many biological fields, including cancer research. Cancer results from the multistep accumulation of mutations that confer to the transformed cells certain biological hallmarks typical of the malignant phenotype. One of the major goals in cancer research is ...Read More
Cui Jianzhou Chew ShirleyJiaLi Shi Yin Gong Zhiyuan Shen Han-Ming
B M B Reports 2017-03-01
CRISPR/Cas9 is the latest tool introduced in the field of genome engineering and is so far the best genome-editing tool as compared to its precedents such as, meganucleases, zinc finger nucleases (ZFNs) and transcription activator-like effectors (TALENs). The simple design and assembly of the CRISPR/Cas9 system makes genome edit...Read More
Hough SorenH Kancleris Kris Brody Leigh Humphryes-Kirilov Neil Wolanski Joseph Dunaway Keith Ajetunmobi Ayokunmi Dillard Victor
BMC Bioinformatics 2017-03-01
Guide Picker ( ) serves as a meta tool for designing CRISPR experiments by presenting ten different guide RNA scoring functions in one simple graphical interface. It allows investigators to simultaneously visualize and sort through every guide targeting the protein-coding regions of any mous...Read More
Kick Leonhard Kirchner Marion Schneider Sabine
Bioengineered 2017-03-01
The adaptive bacterial immune system CRISPR-Cas is revolutionizing all fields of life science and has opened up new frontiers toward personalised medicine. Since the elucidation of the molecular mechanism of Cas9 from Streptococcus pyogenes in 2012 and its development as a genomic engineering tool, genetic modifications in more ...Read More
Shi Zhong-Dong Soh Chew-Li Zhu Zengrong Huangfu Danwei
Journal of Visualized Experiments 2017-03-01
Interrogating gene function in self-renewing or differentiating human pluripotent stem cells (hPSCs) offers a valuable platform towards understanding human development and dissecting disease mechanisms in a dish. To capitalize on this potential application requires efficient genome-editing tools to generate hPSC mutants in disea...Read More
Cerikan Berati Schiebel Elmar
Small GTPases 2017-03-01
DOCK6 is a RAC1/CDC42 guanine nucleotide exchange factor, however, little is known about its function and sub-cellular localization. DOCK6 regulates the balance between RAC1 and RHOA activity during cell adhesion and is important for CDC42-dependent mitotic chromosome alignment. Surprisingly, a cell intrinsic adaptation mechanis...Read More
Chen Xiugui Lu Xuke Shu Na Wang Shuai Wang Junjuan Wang Delong Guo Lixue Ye Wuwei
Scientific reports 2017-03-01
The CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats)/Cas9 system has been widely used for genome editing in various plants because of its simplicity, high efficiency and design flexibility. However, to our knowledge, there is no report on the application of CRISPR/Cas9-mediated targeted mutagenesis in cotton. H...Read More
Wrona Dominik Siler Ulrich Reichenbach Janine
Scientific reports 2017-03-01
Development of gene therapy vectors requires cellular models reflecting the genetic background of a disease thus allowing for robust preclinical vector testing. For human p47(phox)-deficient chronic granulomatous disease (CGD) vector testing we generated a cellular model using clustered regularly interspaced short palindromic re...Read More
Wenderoth Maximilian Pinecker Christoph Vo Benjamin Fischer Reinhard
Fungal Genetics and Biology 2017-03-01
The filamentous fungus Alternaria alternata is a potent producer of many secondary metabolites, some of which like alternariol or alternariol-methyl ether are toxic and/or cancerogenic. Many Alternaria species do not only cause post-harvest losses of food and feed, but are aggressive plant pathogens. Despite the great economic i...Read More
Cruz-Molina Sara Respuela Patricia Tebartz Christina Kolovos Petros Nikolic Milos Fueyo Raquel vanIjcken WilfredFJ Grosveld Frank Frommolt Peter Bazzi Hisham Rada-Iglesias Alvaro
Cell Stem Cell 2017-03-01
Poised enhancers marked by H3K27me3 in pluripotent stem cells have been implicated in the establishment of somatic expression programs during embryonic stem cell (ESC) differentiation. However, the functional relevance and mechanism of action of poised enhancers remain unknown. Using CRISPR/Cas9 technology to engineer precise ge...Read More
Xu Mingchu Xie YajingAngela Abouzeid Hana Gordon ChristopherT Fiorentino Alessia Sun Zixi Lehman Anna Osman IhabS Dharmat Rachayata Riveiro-Alvarez Rosa Bapst-Wicht Linda Babino Darwin Arno Gavin Busetto Virginia Zhao Li Li Hui Lopez-Martinez MiguelA Azevedo LilianaF Hubert Laurence Pontikos Nikolas Eblimit Aiden Lorda-Sanchez Isabel Kheir Valeria Plagnol Vincent Oufadem Myriam Soens ZachryT Yang Lizhu Bole-Feysot Christine Pfundt Rolph Allaman-Pillet Nathalie Nitschk Patrick Cheetham MichaelE Lyonnet Stanislas Agrawal SmritiA Li Huajin Pinton Gatan Michaelides Michel Besmond Claude Li Yumei Yuan Zhisheng vonLintig Johannes Webster AndrewR LeHir Herv Stoilov Peter Amiel Jeanne Hardcastle AlisonJ Ayuso Carmen Sui Ruifang Chen Rui Allikmets Rando Schorderet DanielF
The American Journal of Human Genetics 2017-03-01
Pre-mRNA splicing factors play a fundamental role in regulating transcript diversity both temporally and spatially. Genetic defects in several spliceosome components have been linked to a set of non-overlapping spliceosomopathy phenotypes in humans, among which skeletal developmental defects and non-syndromic retinitis pigmentos...Read More
Wang Hongcheng Luo Lingfei Yang Deqin
Biochemical and Biophysical Research Communications 2017-03-01
The cranial vasculature is crucial for the survival and development of the central nervous system and is closely related to brain pathologies. Characterizations of the underlying mechanisms by which cranial vessels acquire their stereotypic patterning remain to be the key interest in the cerebrovascular research. In this report,...Read More
Huo Xueyun Du Yating Lu Jing Guo Meng Li Zhenkun Zhang Shuangyue Li Xiaohong Chen Zhenwen Du Xiaoyan
Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR- associated (Cas) protein 9 system is a novel and powerful tool which is widely used for genome editing. CRISPR/Cas9 is RNA-guided and can lead to desired genomic modifications. However, whether the CRISPR/Cas9-mediated genome editing causes genomic altera...Read More
Rossi CiroCsar daSilvaDias Ingrid Muniz IgorMansur Lilenbaum Walter Giambiagi-deMarval Marcia
Veterinary Microbiology 2017-03-01
This study aimed to characterize the species, antimicrobial resistance and dispersion of CRISPR systems in staphylococci isolated from the oropharynx of domestic cats in Brazil. Staphylococcus strains (n=75) were identified by MALDI-TOF and sequencing of rpoB and tuf genes. Antimicrobial susceptibility was assessed by disk diffu...Read More
Wang Yanliang Li Junhong Xiang Jinzhu Wen Bingqiang Mu Haiyuan Zhang Wei Han Jianyong
Protein & Cell 2017-03-01
Suzuki HiroshiI Young RichardA Sharp PhillipA
Cell 2017-03-01
Super-enhancers are an emerging subclass of regulatory regions controlling cell identity and disease genes. However, their biological function and impact on miRNA networks are unclear. Here, we report that super-enhancers drive the biogenesis of master miRNAs crucial for cell identity by enhancing both transcription and Drosha/D...Read More
Xu Yongru Qi Yingzi Luo Jing Yang Jing Xie Qi Deng Chen Su Na Wei Wei Shi Deshun Xu Feng Li Xiangping Xu Ping
International Journal of Molecular Sciences 2017-03-01
Chronic hepatitis B virus (HBV) infection has been considered as the major cause of hepatocellular carcinoma (HCC). Hepatitis B virus X protein (HBx) has been reported to be oncogenic. The underlying mechanisms of HBV-related HCC are not fully understood, and the role played by the HBx protein in HBV induced carcinogenesis remai...Read More
Tian Jingjing Hu Jia Chen Mingli Yin Huancai Miao Peng Bai Pengli Yin Jian
Aquatic Toxicology 2017-03-01
Previous studies in our lab have revealed that both P-glycoprotein (Pgp) and multi-resistance associated protein (Mrp) 1 played important roles in the detoxification of heavy metals and polycyclic aromatic hydrocarbon (PAH) in zebrafish embryos. This paper aims to extend this research by using mrp1-deficient model to illustrate ...Read More
Pazhakh Vahid Clark Sharon Keightley MCristina Lieschke GrahamJ
Scientific reports 2017-03-01
Granulocyte colony-stimulating factor (GCSF) and its receptor (GCSFR), also known as CSF3 and CSF3R, are required to maintain normal neutrophil numbers during basal and emergency granulopoiesis in humans, mice and zebrafish. Previous studies identified two zebrafish CSF3 ligands and a single CSF3 receptor. Transient antisense mo...Read More
Peterson KevinA Beane GlenL Goodwin LeslieO Kutny PeterM Reinholdt LauraG Murray StephenA
Mammalian Genome 2017-03-01
Genome editing using the CRISPR/Cas9 RNA-guided endonuclease system has rapidly become a driving force for discovery in modern biomedical research. This simple yet elegant system has been widely used to generate both loss-of-function alleles and precision knock-in mutations using single-stranded donor oligonucleotides. Our CRISP...Read More
ElMouridi Sonia Lecroisey Claire Tardy Philippe Mercier Marine Leclercq-Blondel Alice Zariohi Nora Boulin Thomas
G3: Genes, Genomes, Genetics 2017-03-01
CRISPR/Cas9 genome engineering strategies allow the directed modification of the Caenorhabditis elegans genome to introduce point mutations, generate knock-out mutants, and insert coding sequences for epitope or fluorescent tags. Three practical aspects, however, complicate such experiments. First, the efficiency and specificity...Read More
Xie Ze-Xiong Li Bing-Zhi Mitchell LeslieA Wu Yi Qi Xin Jin Zhu Jia Bin Wang Xia Zeng Bo-Xuan Liu Hui-Min Wu Xiao-Le Feng Qi Zhang Wen-Zheng Liu Wei Ding Ming-Zhu Li Xia Zhao Guang-Rong Qiao Jian-Jun Cheng Jing-Sheng Zhao Meng Kuang Zheng Wang Xuya Martin JAndrew Stracquadanio Giovanni Yang Kun Bai Xue Zhao Juan Hu Meng-Long Lin Qiu-Hui Zhang Wen-Qian Shen Ming-Hua Chen Si Su Wan Wang En-Xu Guo Rui Zhai Fang Guo Xue-Jiao Du Hao-Xing Zhu Jia-Qing Song Tian-Qing Dai Jun-Jun Li Fei-Fei Jiang Guo-Zhen Han Shi-Lei Liu Shi-Yang Yu Zhi-Chao Yang Xiao-Na Chen Ken Hu Cheng Li Da-Shuai Jia Nan Liu Yue Wang Lin-Ting Wang Su Wei Xiao-Tong Fu Mei-Qing Qu Lan-Meng Xin Si-Yu Liu Ting Tian Kai-Ren Li Xue-Nan Zhang Jin-Hua Song Li-Xiang Liu Jin-Gui Lv Jia-Fei Xu Hang Tao Ran Wang Yan Zhang Ting-Ting Deng Ye-Xuan Wang Yi-Ran Li Ting Ye Guang-Xin Xu Xiao-Ran Xia Zheng-Bao Zhang Wei Yang Shi-Lan Liu Yi-Lin Ding Wen-Qi Liu Zhen-Ning Zhu Jun-Qi Liu Ning-Zhi Walker Roy Luo Yisha Wang Yun Shen Yue Yang Huanming Cai Yizhi Ma Ping-Sheng Zhang Chun-Ting Bader JoelS Boeke JefD Yuan Ying-Jin
Science 2017-03-01
Perfect matching of an assembled physical sequence to a specified designed sequence is crucial to verify design principles in genome synthesis. We designed and de novo synthesized 536,024-base pair chromosome synV in the Build-A-Genome China course. We corrected an initial isolate of synV to perfectly match the designed sequence...Read More
Zagorski JosephW Maser TylerP Liby KarenT Rockwell CherylE
Journal of Pharmacology and Experimental Therapeutics 2017-03-01
Nuclear factor erythroid 2-related factor 2 (Nrf2) is a stress-activated transcription factor activated by stimuli such as electrophilic compounds and other reactive xenobiotics. Previously, we have shown that the commonly used food additive and Nrf2 activator tert-butylhydroquinone (tBHQ) suppresses interleukin-2 (IL-2) product...Read More
Ali Amjad Raja Rameez Farooqui SabihurRahman Ahmad Shaista Banerjea AkhilC
Biochemical Journal 2017-03-01
Deubiquitinases (DUBs) are key regulators of complex cellular processes. HIV-1 Tat is synthesized early after infection and is mainly responsible for enhancing viral production. Here, we report that one of the DUBs, USP7, stabilized the HIV-1 Tat protein through its deubiquitination. Treatment with either a general DUB inhibitor...Read More
Stallaert Wayne vanderWesthuizen EmmaT Schnegge Anne-Marie Plouffe Bianca Hogue Mireille Lukashova Viktoria Inoue Asuka Ishida Satoru Aoki Junken LeGouill Christian Bouvier Michel
Molecular Pharmacology 2017-03-01
The ?2 adrenergic receptor (?2AR) increases intracellular Ca(2+) in a variety of cell types. By combining pharmacological and genetic manipulations, we reveal a novel mechanism through which the ?2AR promotes Ca(2+) mobilization (pEC50 = 7.32 0.10) in nonexcitable human embryonic kidney (HEK)293S cells. Downregulation of Gs wit...Read More
Prykhozhij SergeyV Steele ShelbyL Razaghi Babak Berman JasonN
Disease Models & Mechanisms 2017-03-01
Clustered Regularly Interspaced Palindromic Repeats (CRISPR)/Cas9 adaptive immunity against pathogens in bacteria has been adapted for genome editing and applied in zebrafish (Danio rerio) to generate frameshift mutations in protein-coding genes. Although there are methods to detect, quantify and sequence CRISPR/Cas9-induced mut...Read More
Li Zhihua Bi Yanwei Xiao Hongjian Sun Le Ren Yuan Li Yadong Chen Chen Cun Wei
Virus Research 2017-03-01
The CRISPR-Cas9 (clustered regularly interspaced short palindromic repeats and CRISPR-associated protein 9) system has been widely used for viral genome editing, transcription regulation and chromosomal localization in eukaryotic cells. In this study, a guide RNA (gRNA) that specifically recognizes HSV-1 viral genomes was used i...Read More
Gerace Dario Martiniello-Wilks Rosetta Nassif NajahTherese Lal Sara Steptoe Raymond Simpson AnnMargaret
Stem Cell Research & Therapy 2017-03-01
Due to their ease of isolation, differentiation capabilities, and immunomodulatory properties, the therapeutic potential of mesenchymal stem cells (MSCs) has been assessed in numerous pre-clinical and clinical settings. Currently, whole pancreas or islet transplantation is the only cure for people with type 1 diabetes (T1D) and,...Read More
Faddaoui Adnen Sheta Razan Bachvarova Magdalena Plante Marie Gregoire Jean Renaud Marie-Claude Sebastianelli Alexandra Gobeil Stephane Morin Chantale Ghani Karim Bachvarov Dimcho
Cell Cycle 2017-03-01
Previously, we have identified the Grainyhead transcription factor 2 gene (GRHL2) as notably hypomethylated in high-grade (HG) serous epithelial ovarian tumors, compared with normal ovarian tissues. GRHL2 is known for its functions in normal tissue development and wound healing. In the context of cancer, the role of GRHL2 is sti...Read More
Tsui TszKinMartin Hand TravisH Duboy EmilyC Li Hong
ACS Synthetic Biology 2017-03-01
Cas9 is an RNA-guided DNA cleavage enzyme being actively developed for genome editing and gene regulation. To be cleaved by Cas9, a double stranded DNA, or the protospacer, must be complementary to the guide region, typically 20-nucleotides in length, of the Cas9-bound guide RNA, and adjacent to a short Cas9-specific element cal...Read More
Das Dipon Smith Nathan Wang Xu Morgan IainM
Journal of Virology 2017-03-01
Human papillomaviruses (HPV) replicate their genomes in differentiating epithelium using the viral proteins E1 and E2 in association with host proteins. While the roles of E1 and E2 in this process are understood, the host factors involved and how they interact with and regulate E1-E2 are not. Our previous work identified the ho...Read More
Gui Hongsheng Schriemer Duco Cheng WilliamW Chauhan RajendraK Anti?olo Guillermo Berrios Courtney Bleda Marta Brooks AliceS Brouwer RutgerWW Burns AlanJ Cherny StaceyS Dopazo Joaquin Eggen BartJL Griseri Paola Jalloh Binta Le Thuy-Linh Lui VincentCH Luzn-Toro Berta Matera Ivana Ngan EllySW Pelet Anna Ruiz-Ferrer Macarena Sham PakC Shepherd IainT So Man-Ting Sribudiani Yunia Tang ClaraSM vandenHout MirjamCGN vanderLinde HermaC vanHam TjakkoJ vanIJcken WilfredFJ Verheij JokeBGM Amiel Jeanne Borrego Salud Ceccherini Isabella Chakravarti Aravinda Lyonnet Stanislas Tam PaulKH Garcia-Barcel Maria-Merc Hofstra RobertMW
Genome Biology 2017-03-01
Hirschsprung disease (HSCR), which is congenital obstruction of the bowel, results from a failure of enteric nervous system (ENS) progenitors to migrate, proliferate, differentiate, or survive within the distal intestine. Previous studies that have searched for genes underlying HSCR have focused on ENS-related pathways and genes...Read More
Xu Xiaojun Duan Dongsheng Chen Shi-Jie
Scientific reports 2017-03-01
The CRISPR/Cas9 complex, a bacterial immune response system, has been widely adopted for RNA-guided genome editing and transcription regulation in applications such as targeted genome modification and site-directed mutagenesis. However, the physical basis for its target specificity is not fully understood. In this study, based o...Read More
Ma Teng Tao Jingli Yang Minghui He Changjiu Tian Xiuzhi Zhang Xiaosheng Zhang Jinlong Deng Shoulong Feng Jianzhong Zhang Zhenzhen Wang Jing Ji Pengyun Song Yukun He Pingli Han Hongbing Fu Juncai Lian Zhengxing Liu Guoshi
Journal of Pineal Research 2017-03-01
Melatonin as a potent antioxidant exhibits important nutritional and medicinal values. To produce melatonin-enriched milk will benefit the consumers. In this study, a sheep bioreactor which generates melatonin-enriched milk has been successfully developed by the technology that combined CRISPR/Cas9 system and microinjection. The...Read More
Morita Hitoshi Taimatsu Kiyohito Yanagi Kanoko Kawahara Atsuo
Bioengineered 2017-03-01
Genome editing technologies, such as transcription activator-like effector nuclease (TALEN) and the clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein (Cas) systems, can induce DNA double-strand breaks (DSBs) at the targeted genomic locus, leading to frameshift-mediated gene disruption in...Read More
March OliverP Reichelt Julia Koller Ulrich
Experimental Physiology 2017-03-01
What is the topic of this review? This review concerns current gene editing strategies for blistering skin diseases with respect to individual genetic constellations and distinct conditions. What advances does it highlight? Specificity and safety dominate the discussion of gene editing applications for gene therapy, where a numb...Read More
Ackermann AmandaM Zhang Jia Heller Aryel Briker Anna Kaestner KlausH
Molecular Metabolism 2017-03-01
?-cells are the second most prominent cell type in pancreatic islets and are responsible for producing glucagon to increase plasma glucose levels in times of fasting. ?-cell dysfunction and inappropriate glucagon secretion occur in both type 1 and type 2 diabetes. Thus, there is growing interest in studying both normal function ...Read More
Nigro OliviaD Jungbluth SeanP Lin Huei-Ting Hsieh Chih-Chiang Miranda JaclynA Schvarcz ChristopherR Rapp MichaelS Steward GriegF
mBio 2017-03-01
Microbial life has been detected well into the igneous crust of the seafloor (i.e., the oceanic basement), but there have been no reports confirming the presence of viruses in this habitat. To detect and characterize an ocean basement virome, geothermally heated fluid samples (ca. 60 to 65C) were collected from 117 to 292m deep ...Read More
Bu Qian Wang Anxin Hamzah Hamdi Waldman Alex Jiang Keer Dong Qiping Li Ronghui Kim Jason Turner Daniel Chang Qiang
Journal of Neuroscience 2017-03-01
Rett syndrome (RTT) is a debilitating neurodevelopmental disorder caused by mutations in the MECP2 gene. To facilitate the study of cellular mechanisms in human cells, we established several human stem cell lines: human embryonic stem cell (hESC) line carrying the common T158M mutation (MECP2(T158M/T158M) ), hESC line expressing...Read More
Rahman Sunniyat Magnussen Michael Len TheresaE Farah Nadine Li Zhaodong Abraham BrianJ Alapi KrisztinaZ Mitchell RachelJ Naughton Tom Fielding AdeleK Pizzey Arnold Bustraan Sophia Allen Christopher Popa Teodora Pike-Overzet Karin Garcia-Perez Laura Gale RosemaryE Linch DavidC Staal FrankJT Young RichardA Look AThomas Mansour MarcR
Blood 2017-03-01
Somatic mutations within non-coding genomic regions that aberrantly activate oncogenes have remained poorly characterized. Here we describe recurrent activating intronic mutations of LMO2, a prominent oncogene in T-cell acute lymphoblastic leukemia (T-ALL). Heterozygous mutations were identified in PF-382 and DU.528 T-ALL cell l...Read More
Kohn DonaldB Kuo CarolineY
Journal of Allergy and Clinical Immunology 2017-03-01
The most severe primary immune deficiency diseases (PIDs) have been successfully treated with allogeneic hematopoietic stem cell transplantation for more than 4decades. However, such transplantations have the best outcomes when there is a well-matched donor available because immune complications, such as graft-versus-host diseas...Read More
Villate-Beitia Ilia Zarate Jon Puras Gustavo Pedraz JosLuis
Drug Development and Industrial Pharmacy 2017-03-01
Cystic fibrosis (CF) is a monogenic autosomal recessive disorder where the defective gene, the cystic fibrosis transmembrane conductance regulator (CFTR), is well identified. Moreover, the respiratory tract can be targeted through noninvasive aerosolized formulations for inhalation. Therefore, gene therapy is considered a plausi...Read More
Hcker Irina HarrellIi RobertA Eichner Gerrit Pilitt KristinaL O'Brochta DavidA Handler AlfredM Schetelig MarcF
Scientific reports 2017-03-01
Site-specific genome modification (SSM) is an important tool for mosquito functional genomics and comparative gene expression studies, which contribute to a better understanding of mosquito biology and are thus a key to finding new strategies to eliminate vector-borne diseases. Moreover, it allows for the creation of advanced tr...Read More
Chen Jinlin Jiang Dongneng Tan Dejie Fan Zheng Wei Yingying Li Minghui Wang Deshou
Scientific reports 2017-03-01
Eukaryotic elongation factor 1 alpha (eEF1A) is an essential component of the translational apparatus. In the present study, eEF1A1b was isolated from the Nile tilapia. Real-time PCR and Western blot revealed that eEF1A1b was expressed highly in the testis from 90 dah (days after hatching) onwards. In situ hybridization and immu...Read More
Birling Marie-Christine Schaeffer Laurence Andr Philippe Lindner Loic Marchal Damien Ayadi Abdel Sorg Tania Pavlovic Guillaume Hrault Yann
Scientific reports 2017-03-01
Modelling Down syndrome (DS) in mouse has been crucial for the understanding of the disease and the evaluation of therapeutic targets. Nevertheless, the modelling so far has been limited to the mouse and, even in this model, generating duplication of genomic regions has been labour intensive and time consuming. We developed the ...Read More
Rajagopalan Ramya Kroos Lee
Journal of Bacteriology 2017-03-01
Myxococcus xanthus undergoes multicellular development when starved. Thousands of rod-shaped cells coordinate their movements and aggregate into mounds in which cells differentiate into spores. Mutations in the dev operon impair development. The dev operon encompasses a clustered regularly interspaced short palindromic repeat-as...Read More
Carpenter MeganR Kalburge SaiS Borowski JosephD Peters MollyC Colwell RitaR Boyd EFidelma
Journal of Bacteriology 2017-03-01
Pathogenicity islands (PAIs) are mobile integrated genetic elements that contain a diverse range of virulence factors. PAIs integrate into the host chromosome at a tRNA locus that contains their specific bacterial attachment site, attB, via integrase-mediated site-specific recombination generating attL and attR sites. We identif...Read More
Bekpen Cemalettin Knzel Sven Xie Chen Eaaswarkhanth Muthukrishnan Lin Yen-Lung Gokcumen Omer Akdis CezmiA Tautz Diethard
BMC Genomics 2017-03-01
Segmental duplications are an abundant source for novel gene functions and evolutionary adaptations. This mechanism of generating novelty was very active during the evolution of primates particularly in the human lineage. Here, we characterize the evolution and function of the SPATA31 gene family (former designation FAM75A), whi...Read More
Sweeney ColinL Choi Uimook Liu Chengyu Koontz Sherry Ha Seung-Kwon Malech HarryL
Human Gene Therapy 2017-03-01
Chronic granulomatous disease (CGD) is characterized by defects in the production of microbicidal reactive oxygen species (ROS) by phagocytes. Testing of gene and cell therapies for the treatment of CGD in human hematopoietic cells requires preclinical transplant models. The use of the lymphocyte-deficient NOD.Cg-Prkdc(scid) Il2...Read More
Schmidt StephanieTzouanas Zimmerman StephanieM Wang Jianbin Kim StuartK Quake StephenR
ACS Synthetic Biology 2017-03-01
Lineage tracing by the determination and mapping of progeny arising from single cells is an important approach enabling the elucidation of mechanisms underlying diverse biological processes ranging from development to disease. We developed a dynamic sequence-based barcode system for synthetic lineage tracing and have demonstrate...Read More
Wefers Benedikt Bashir Sanum Rossius Jana Wurst Wolfgang Khn Ralf
Methods 2017-03-01
The generation of targeted mouse mutants is a key technology for biomedical research. Using the CRISPR/Cas9 system for induction of targeted double-strand breaks, gene editing can be performed in a single step directly in mouse zygotes. This article covers the design of knockout and knockin alleles, preparation of reagents, micr...Read More
Mout Rubul Ray Moumita Lee Yi-Wei Scaletti Federica Rotello VincentM
Bioconjugate Chemistry 2017-03-01
The successful use of clustered regularly interspaced short palindromic repeat (CRISPR)/Cas9-based gene editing for therapeutics requires efficient in vivo delivery of the CRISPR components. There are, however, major challenges on the delivery front. In this Topical Review, we will highlight recent developments in CRISPR deliver...Read More
Perez AlexendarR Pritykin Yuri Vidigal JoanaA Chhangawala Sagar Zamparo Lee Leslie ChristinaS Ventura Andrea
Nature Biotechnology 2017-03-01
We present GuideScan software for the design of CRISPR guide RNA libraries that can be used to edit coding and noncoding genomic regions. GuideScan produces high-density sets of guide RNAs (gRNAs) for single- and paired-gRNA genome-wide screens. We also show that the trie data structure of GuideScan enables the design of gRNAs t...Read More
Achigar Rodrigo Magadn AlfonsoH Tremblay DeniseM JuliaPianzzola Mara Moineau Sylvain
Scientific reports 2017-03-01
Three cos-type virulent Streptococcus thermophilus phages were isolated from failed mozzarella production in Uruguay. Genome analyses showed that these phages are similar to those isolated elsewhere around the world. The CRISPR1 and CRISPR3 arrays of the three S. thermophilus host strains from Uruguay were also characterized and...Read More
Sonoiki Ebere Ng CarolineL Lee MarcusCS Guo Denghui Zhang Yong-Kang Zhou Yasheen Alley MRK Ahyong Vida Sanz LauraM Lafuente-Monasterio MariaJose Dong Chen Schupp PatrickG Gut Jiri Legac Jenny Cooper RolandA Gamo Francisco-Javier DeRisi Joseph Freund YvonneR Fidock DavidA Rosenthal PhilipJ
Nature Communications 2017-03-01
Benzoxaboroles are effective against bacterial, fungal and protozoan pathogens. We report potent activity of the benzoxaborole AN3661 against Plasmodium falciparum laboratory-adapted strains (mean IC50 32?nM), Ugandan field isolates (mean ex vivo IC50 64?nM), and murine P. berghei and P. falciparum infections (day 4 ED90 0.34 an...Read More
Xu XQ Xin YQ Li X Zhang QW Yang XY Jin Y Zhao HH Jin X Qi ZZ
Zhonghua yu fang yi xue za zhi [Chinese journal of preventive medicine] 2017-03-01
Objective: To investigate the CRISPR genotypes (clusters) and regional distribution of Yersinia pestis in Qinghai-plateau. Methods: One hundred and two isolates of Y. pestis isolated from human plague patients, host animal and insect vectors from Qinghai-plateau were selected. The DNAs were extracted using the traditional sodium...Read More
Ren Bin Yan Fang Kuang Yongjie Li Na Zhang Dawei Lin Honghui Zhou Huanbin
SCIENCE CHINA Life Science 2017-03-01
Lin Lin Petersen TrineSkov Jensen KristopherTorp Bolund Lars Khn Ralf Luo Yonglun
Journal of Biotechnology 2017-03-01
Mammalian cells repair double-strand DNA breaks (DSB) by a range of different pathways following DSB induction by the engineered clustered regularly interspaced short palindromic repeats (CRISPR)-associated protein Cas9. While CRISPR-Cas9 thus enables predesigned modifications of the genome, applications of CRISPR-Cas9-mediated ...Read More
Fan Hueng-Chuen Chi Ching-Shiang Lee Yih-Jing Tsai Jeng-Dau Lin Shinn-Zong Harn Horng-Jyh
Cell Transplantation 2017-03-01
Neurodegenerative diseases (NDs), at least including Alzheimer's, Huntington's, and Parkinson's diseases, have become the most dreaded maladies because of no precise diagnostic tools or definite treatments for these debilitating diseases. The increased prevalence and a substantial impact on the social-economic and medical care o...Read More
Janga MadhusudhanaR Campbell LeAnneM Rathore KeertiS
Plant Molecular Biology 2017-03-01
The clustered, regularly interspaced, short palindromic repeats (CRISPR)/CRISPR associated (Cas)9 protein system has emerged as a simple and efficient tool for genome editing in eukaryotic cells. It has been shown to be functional in several crop species, yet there are no reports on the application of this or any other genome ed...Read More
Holsclaw JulieKorda Sekelsky Jeff
Genetics 2017-03-01
DNA double-strand breaks (DSBs) pose a serious threat to genomic integrity. If unrepaired, they can lead to chromosome fragmentation and cell death. If repaired incorrectly, they can cause mutations and chromosome rearrangements. DSBs are repaired using end-joining or homology-directed repair strategies, with the predominant for...Read More
Wang Shaohua Dong Sheng Wang Pixiang Tao Yong Wang Yi
Applied and Environmental Microbiology 2017-03-01
Clostridium saccharoperbutylacetonicum N1-4 is well known as a hyper-butanol-producing strain. However, the lack of genetic engineering tools hinders further elucidation of its solvent production mechanism and development of more robust strains. In this study, we set out to develop an efficient genome engineering system for this...Read More
Wang Ying Naturale VictorF Adler PaulN
G3: Genes, Genomes, Genetics 2017-03-01
The Planar cell Polarity Effector (PPE) genes inturned, fuzzy, and fritz are downstream components in the frizzled/starry night signaling pathway, and their function is instructed by upstream Planar Cell Polarity (PCP) core genes such as frizzled and dishevelled PPE proteins accumulate asymmetrically in wing cells and function i...Read More
Zheng Xuan Li Shi-Yuan Zhao Guo-Ping Wang Jin
Biochemical and Biophysical Research Communications 2017-03-01
Accompanied with the internal non-homologous end joining (NHEJ) system, Cas9 can be used to easily inactivate a gene or delete a fragment through introduction of DNA double-stranded breaks (DSBs) in eukaryotic cells. While in most prokaryotes (e.g. Escherichia coli), due to the lack of NHEJ, homologous recombination (HR) is requ...Read More
Liu Weijun Chen Hanxiang Wong Nathan Haynes Wesley Baker CallieM Wang Xiaowei
Cancer Letters 2017-03-01
Pseudohypoxia plays a central role in the progression and therapeutic resistance of clear cell renal cell carcinoma (ccRCC); however, the underlying mechanisms are poorly understood. MicroRNA miR-126 has decreased expression in metastatic or relapsed ccRCC as compared to primary tumors, but the mechanisms by which miR-126 is imp...Read More
Li Chao Unver Turgay Zhang Baohong
Scientific reports 2017-03-01
The complex allotetraploid genome is one of major challenges in cotton for repressing gene expression. Developing site-specific DNA mutation is the long-term dream for cotton breeding scientists. The clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9 (CRISPR/Cas9) system is emerging as a robust...Read More
Ferry QuentinRV Lyutova Radostina Fulga TudorA
Nature Communications 2017-03-01
CRISPR-based transcription regulators (CRISPR-TRs) have transformed the current synthetic biology landscape by allowing specific activation or repression of any target gene. Here we report a modular and versatile framework enabling rapid implementation of inducible CRISPR-TRs in mammalian cells. This strategy relies on the desig...Read More
Li Bin Cui Guanghong Shen Guoan Zhan Zhilai Huang Luqi Chen Jiachun Qi Xiaoquan
Scientific reports 2017-03-01
CRISPR/Cas9 is a powerful genome editing tool that has been extensively used in model plants and crops, such as Arabidopsis thaliana, rice, wheat, and soybean. Here, we report the use of CRISPR/Cas9 to precisely knock out the committed diterpene synthase gene (SmCPS1) involved in tanshinone biosynthesis in Salvia miltiorrhiza, a...Read More
Hallmann Anna-Lena Arazo-Bravo MarcosJ Mavrommatis Lampros Ehrlich Marc Rpke Albrecht Brockhaus Johannes Missler Markus Sterneckert Jared Schler HansR Kuhlmann Tanja Zaehres Holm Hargus Gunnar
Scientific reports 2017-03-01
Astroglial pathology is seen in various neurodegenerative diseases including frontotemporal dementia (FTD), which can be caused by mutations in the gene encoding the microtubule-associated protein TAU (MAPT). Here, we applied a stem cell model of FTD to examine if FTD astrocytes carry an intrinsic propensity to degeneration and ...Read More
Guo Minzhe Tomoshige Koichi Meister Michael Muley Thomas Fukazawa Takuya Tsuchiya Tomoshi Karns Rebekah Warth Arne Fink-Baldauf IrisM Nagayasu Takeshi Naomoto Yoshio Xu Yan Mall MarcusA Maeda Yutaka
Embo Molecular Medicine 2017-03-01
Though invasive mucinous adenocarcinoma of the lung (IMA) is pathologically distinctive, the molecular mechanism driving IMA is not well understood, which hampers efforts to identify therapeutic targets. Here, by analyzing gene expression profiles of human and mouse IMA, we identified a Mucinous Lung Tumor Signature of 143 genes...Read More
Doetschman Thomas Georgieva Teodora
Circulation Research 2017-03-01
Genetic engineering of model organisms and cultured cells has for decades provided important insights into the mechanisms underlying cardiovascular development and disease. In the past few years the development of several nuclease systems has broadened the range of model/cell systems that can be engineered. Of these, the CRISPR ...Read More
Seeger Timon Porteus Matthew Wu JosephC
Circulation Research 2017-03-01
Pulido-Quetglas Carlos Aparicio-Prat Estel Arnan Carme Polidori Taisia Hermoso Toni Palumbo Emilio Ponomarenko Julia Guigo Roderic Johnson Rory
PLoS computational biology 2017-03-01
CRISPR-Cas9 technology can be used to engineer precise genomic deletions with pairs of single guide RNAs (sgRNAs). This approach has been widely adopted for diverse applications, from disease modelling of individual loci, to parallelized loss-of-function screens of thousands of regulatory elements. However, no solution has been ...Read More
Peng Nan Han Wenyuan Li Yingjun Liang Yunxiang She Qunxin
SCIENCE CHINA Life Science 2017-03-01
Archaea represents the third domain of life, with the information-processing machineries more closely resembling those of eukaryotes than the machineries of the bacterial counterparts but sharing metabolic pathways with organisms of Bacteria, the sister prokaryotic phylum. Archaeal organisms also possess unique features as revea...Read More
Maayah ZaidH Althurwi HassanN El-Sherbeni AhmedA Abdelhamid Ghada Siraki ArnoG El-Kadi AymanOS
Molecular and Cellular Biochemistry 2017-03-01
Numerous experimental studies have demonstrated the role of cytochrome P450 1B1 (CYP1B1) and its associated mid-chain hydroxyeicosatetraenoic acids (mid-chain HETEs) metabolite in the pathogenesis of cardiac hypertrophy. However, the ability of isoproterenol (ISO) to induce cardiac hypertrophy through mid-chain HETEs has not bee...Read More
Tang Lichun Zeng Yanting Du Hongzi Gong Mengmeng Peng Jin Zhang Buxi Lei Ming Zhao Fang Wang Weihua Li Xiaowei Liu Jianqiao
Molecular Genetics and Genomics 2017-03-01
Previous works using human tripronuclear zygotes suggested that the clustered regularly interspaced short palindromic repeat (CRISPR)/Cas9 system could be a tool in correcting disease-causing mutations. However, whether this system was applicable in normal human (dual pronuclear, 2PN) zygotes was unclear. Here we demonstrate tha...Read More
Wu Weilin Orr-Burks Nichole Karpilow Jon Tripp RalphA
Scientific data 2017-03-01
Rotavirus is a major cause of severe gastroenteritis among very young children. In developing countries, rotavirus is the major cause of mortality in children under five years old, causing up to 20% of all childhood deaths in countries with high diarrheal disease burden, with more than 90% of these deaths occurring in Africa and...Read More
Smollett Katherine Blombach Fabian Reichelt Robert Thomm Michael Werner Finn
Nature Microbiology 2017-03-01
The archaeal transcription apparatus is closely related to the eukaryotic RNA polymerase (RNAP) II system, while archaeal genomes are more similar to bacteria with densely packed genes organized in operons. This makes understanding transcription in archaea vital, both in terms of molecular mechanisms and evolution. Very little i...Read More
Chaudhary Kulbhushan Chattopadhyay Anirudha Pratap Dharmendra
Journal Of Cellular Physiology 2017-03-01
Since the dawn of life there is a never ending strife between bacteria and phages. Both are perpetually changing their strategies to take over each other. CRISPR/Cas is the most widespread defense system used by bacteria against mobile genetic elements (MGEs) such as phages, cojugative palsmids, transoposons, and pathogenicity i...Read More
Yasue Akihiro Kono Hitomi Habuta Munenori Bando Tetsuya Sato Keita Inoue Junji Oyadomari Seiichi Noji Sumihare Tanaka Eiji Ohuchi Hideyo
Scientific reports 2017-03-01
The clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein (Cas) system is a rapid gene-targeting technology that does not require embryonic stem cells. To demonstrate dosage effects of the Pax6 gene on eye formation, we generated Pax6-deficient mice with the CRISPR/Cas system. Eyes of founde...Read More
Kato Tomoko Hara Satoshi Goto Yuji Ogawa Yuya Okayasu Haruka Kubota Souichirou Tamano Moe Terao Miho Takada Shuji
Scientific reports 2017-03-01
The clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein 9 (Cas9) system is a useful tool for creation of mutant mice with mutations mirroring those in human patients. Various methods have been developed for this purpose, including deletions, inversions, and translocations. So far, mutant m...Read More
Nguyen Kien Das Biswajit Dobrowolski Curtis Karn Jonathan
mBio 2017-03-01
We showed previously that the histone lysine methyltransferase (HKMT) H3K27me3 (EZH2) is the catalytic subunit of Polycomb repressive complex 2 (PRC2) and is required for the maintenance of HIV-1 latency in Jurkat T cells. Here we show, by using chromatin immunoprecipitation experiments, that both PRC2 and euchromatic histone-ly...Read More
Zhu Meng Yan Caiwang Ren Chuanli Huang Xiaodan Zhu Xun Gu Haiyong Wang Meilin Wang Shouyu Gao Yong Ji Yong Miao Xiaoping Yang Ming Chen Jinfei Du Jiangbo Huang Tongtong Jiang Yue Dai Juncheng Ma Hongxia Zhou Jianwei Wang Zhaoming Hu Zhibin Ji Guozhong Zhang Zhengdong Shen Hongbing Shi Yongyong Jin Guangfu
Gastroenterology 2017-03-01
Several genetic variants have been associated with gastric cancer risk, although these account for only a fraction of cases of gastric cancer. We aimed to identify low-frequency and other genetic variants that determine gastric cancer susceptibility.Read More
Wolter Felix Puchta Holger
Genome Biology 2017-03-01
Selection-free genome editing using Cas9 ribonucleoprotein embryo bombardment has been achieved for maize and wheat. This is a breakthrough that should make new breeding technologies more acceptable for worldwide use.Read More
Stout Emily Klaenhammer Todd Barrangou Rodolphe
Food Science and Technology 2017-02-01
Clustered regularly interspaced short palindromic repeats (CRISPRs) and CRISPR-associated (Cas) proteins form adaptive immune systems that occur in many bacteria and most archaea. In addition to protecting bacteria from phages and other invasive mobile genetic elements, CRISPR-Cas molecular machines can be repurposed as tool kit...Read More
Kim Kyoungmi Ryu Seuk-Min Kim Sang-Tae Baek Gayoung Kim Daesik Lim Kayeong Chung Eugene Kim Sunghyun Kim Jin-Soo
Nature Biotechnology 2017-02-01
Base editors (BEs) composed of a cytidine deaminase fused to CRISPR-Cas9 convert cytidine to uridine, leading to single-base-pair substitutions in eukaryotic cells. We delivered BE mRNA or ribonucleoproteins targeting the Dmd or Tyr gene via electroporation or microinjection into mouse zygotes. F0 mice showed nonsense mutations ...Read More
Zong Yuan Wang Yanpeng Li Chao Zhang Rui Chen Kunling Ran Yidong Qiu Jin-Long Wang Daowen Gao Caixia
Nature Biotechnology 2017-02-01
Targeted base editing in plants without the need for a foreign DNA donor or double-stranded DNA cleavage would accelerate genome modification and breeding in a wide array of crops. We used a CRISPR-Cas9 nickase-cytidine deaminase fusion to achieve targeted conversion of cytosine to thymine from position 3 to 9 within the protosp...Read More
Dowdy StevenF
Nature Biotechnology 2017-02-01
RNA-based therapeutics, such as small-interfering (siRNAs), microRNAs (miRNAs), antisense oligonucleotides (ASOs), aptamers, synthetic mRNAs and CRISPR-Cas9, have great potential to target a large part of the currently undruggable genes and gene products and to generate entirely new therapeutic paradigms in disease, ranging from...Read More
Lal Shruti Cheung EdwinC Zarei Mahsa Preet Ranjan Chand SaswatiN Mambelli-Lisboa NicoleC Romeo Carmella Stout MatthewC Londin Eric Goetz Austin Lowder CinthyaY Nevler Avinoam Yeo CharlesJ Campbell PaulM Winter JordanM Dixon DanA Brody JonathanR
Molecular Cancer Research 2017-02-01
Pancreatic ductal adenocarcinoma (PDA) is the third leading cause of cancer-related deaths in the United States, whereas colorectal cancer is the third most common cancer. The RNA-binding protein HuR (ELAVL1) supports a pro-oncogenic network in gastrointestinal (GI) cancer cells through enhanced HuR expression. Using a publicall...Read More
Wan Liling Wen Hong Li Yuanyuan Lyu Jie Xi Yuanxin Hoshii Takayuki Joseph JuliaK Wang Xiaolu Loh Yong-HweeE Erb MichaelA Souza AmandaL Bradner JamesE Shen Li Li Wei Li Haitao Allis CDavid Armstrong ScottA Shi Xiaobing
Nature 2017-02-01
Cancer cells are characterized by aberrant epigenetic landscapes and often exploit chromatin machinery to activate oncogenic gene expression programs. Recognition of modified histones by 'reader' proteins constitutes a key mechanism underlying these processes; therefore, targeting such pathways holds clinical promise, as exempli...Read More
Erb MichaelA Scott ThomasG Li BinE Xie Huafeng Paulk Joshiawa Seo Hyuk-Soo Souza Amanda Roberts JustinM Dastjerdi Shiva Buckley DennisL Sanjana NevilleE Shalem Ophir Nabet Behnam Zeid Rhamy Offei-Addo NanaK Dhe-Paganon Sirano Zhang Feng Orkin StuartH Winter GeorgE Bradner JamesE
Nature 2017-02-01
Recurrent chromosomal translocations producing a chimaeric MLL oncogene give rise to a highly aggressive acute leukaemia associated with poor clinical outcome. The preferential involvement of chromatin-associated factors as MLL fusion partners belies a dependency on transcription control. Despite recent progress made in targetin...Read More
Sanz MiguelAngel GonzlezAlmela Esther Carrasco Luis
Scientific reports 2017-02-01
Translation of Sindbis virus subgenomic mRNA (sgmRNA) can occur after inactivation of eIF2 by phosphorylation in mammalian cells. Several studies have suggested that eIF2 can be replaced by eIF2A or eIF2D. HAP1 human cell lines knocked-out for eIF2A, eIF2D or both by CRISPR/Cas9 genome engineering were compared with wild-type (W...Read More
Jordan Bertrand
médecine/sciences 2017-02-01
A novel gene editing procedure based on a nuclease from the Argonaute protein family was described in mid-2016 and appeared to provide significant advantages over the now widely used CRISPR-Cas9 system. Attempts by numerous groups to use this technique have however been unsuccessful; several negative reports have been published ...Read More
Starling Shimona
Nature Reviews: Microbiology 2017-02-01
Xu Xiaohong Tay Yilin Sim Bernice Yoon Su-In Huang Yihui Ooi Jolene Utami KagistiaHana Ziaei Amin Ng Bryan Radulescu Carola Low Donovan Ng AlvinYuJin Loh Marie Venkatesh Byrappa Ginhoux Florent Augustine GeorgeJ Pouladi MahmoudA
Stem cell reports 2017-02-01
Huntington disease (HD) is a dominant neurodegenerative disorder caused by a CAG repeat expansion in HTT. Here we report correction of HD human induced pluripotent stem cells (hiPSCs) using a CRISPR-Cas9 and piggyBac transposon-based approach. We show that both HD and corrected isogenic hiPSCs can be differentiated into excitabl...Read More
Richter Hagen Rompf Judith Wiegel Julia Rau Kristina Randau Lennart
Biochimica et Biophysica Acta 2017-02-01
CRISPR arrays are transcribed into long precursor RNA species, which are further processed into mature CRISPR RNAs (crRNAs). Cas proteins utilize these crRNAs, which contain spacer sequences that can be derived from mobile genetic elements, to mediate immunity during a reoccurring virus infection. Type I CRISPR-Cas systems are d...Read More
Oba Atsushi Shimada Shu Akiyama Yoshimitsu Nishikawaji Taketo Mogushi Kaoru Ito Hiromitsu Matsumura Satoshi Aihara Arihiro Mitsunori Yusuke Ban Daisuke Ochiai Takanori Kudo Atsushi Asahara Hiroshi Kaida Atsushi Miura Masahiko Tanabe Minoru Tanaka Shinji
Journal of Hepatology 2017-02-01
Recent genomic studies have identified frequent mutations of AT-rich interactive domain 2 (ARID2) in hepatocellular carcinoma (HCC), but it is not still understood how ARID2 exhibits tumor suppressor activities.Read More
Schneller JessicaL Lee CiaranM Bao Gang Venditti CharlesP
BMC Medicine 2017-02-01
Inborn errors of metabolism (IEM) include many disorders for which current treatments aim to ameliorate disease manifestations, but are not curative. Advances in the field of genome editing have recently resulted in the in vivo correction of murine models of IEM. Site-specific endonucleases, such as zinc-finger nucleases and the...Read More
Dong Bin Hu He-He Li Zhen-Fang Cheng Rong-Qiang Meng De-Mei Wang Junping Fan Zhen-Chuan
Applied Microbiology and Biotechnology 2017-02-01
Chlamydomonas reinhardtii offers a great promise for large-scale production of multiple recombinant proteins of pharmaceutical and industrial interest. However, the nuclear-encoding transgenes usually are expressed at a low level, which severely hampers the use of this alga in molecular farming. In this study, the promoter of th...Read More
Cozza Giorgio Salvi Mauro Tagliabracci VincentS Pinna LorenzoA
FEBS Journal 2017-02-01
Fam20C, also termed DMP-4 (dentin matrix protein 4) and G-CK (Golgi casein kinase) is an atypical protein kinase committed with the phosphorylation of casein and a plethora of other secreted proteins. Fam20C has been implicated in a number of human pathologies related to biomineralization, phosphate homeostasis, and neoplasia. T...Read More
Ibrahim Maziya Subramanian Ahalyaa Anishetty Sharmila
Functional & Integrative Genomics 2017-02-01
Prevotella is part of the oral bacterial community implicated in periodontitis. Pan genome analyses of eight oral Prevotella species, P. dentalis, P. enoeca, P. fusca, P. melaninogenica, P. denticola, P. intermedia 17, P. intermedia 17-2 and P. sp. oral taxon 299 are presented in this study. Analysis of the Prevotella pan genome...Read More
Oulhen Nathalie Swartz SZachary Laird Jessica Mascaro Alexandra Wessel GaryM
Development 2017-02-01
Stem cells in animals often exhibit a slow cell cycle and/or low transcriptional activity referred to as quiescence. Here, we report that the translational activity in the primordial germ cells (PGCs) of the sea urchin embryo (Strongylocentrotus purpuratus) is quiescent. We measured new protein synthesis with O-propargyl-puromyc...Read More
Makarova KiraS Zhang Feng Koonin EugeneV
Cell 2017-02-01
Class 1 CRISPR-Cas systems are characterized by effector modules consisting of multiple subunits. Class 1 systems comprise about 90% of all CRISPR-Cas loci identified in bacteria and archaea and can target both DNA and RNA.Read More
Zhang Yani Wang Yingjie Zuo Qisheng Li Dong Zhang Wenhui Wang Fei Ji Yanqin Jin Jing Lu Zhenyu Wang Man Zhang Chen Li Bichun
PLoS ONE 2017-02-01
An efficient genome editing approach had been established to construct the stable transgenic cell lines in the domestic chicken (Gallus gallus domesticus) at present. Our objectives were to investigate gene function in the differentiation process of chicken embryonic stem cells (ESCs) into spermatogonial stem cells(SSCs). Three ...Read More
Ahmadiantehrani Somayeh London SarahE
Scientific reports 2017-02-01
Songbird models meaningfully contribute to many fields including learned vocal communication, the neurobiology of social interactions, brain development, and ecology. The value of investigating gene-brain-behavior relationships in songbirds is therefore high. Viral infections typically used in other lab animals to deliver gene e...Read More
Schott Kerstin Knig Renate
Trends in Microbiology 2017-02-01
A new study employs genome-wide loss-of-function CRISPR/Cas9 screening to identify three novel factors for HIV-1 entry. The factors represent promising targets for therapeutics as they are essential for HIV-1 infection, but dispensable for cell survival. The involved pathways were validated in primary CD4(+) T cells, target cell...Read More
Biswas Kuntal Sarkar Sukumar Du Kangping Brautigan DavidL Abbas Tarek Larner JamesM
Molecular Cancer Research 2017-02-01
Lung cancer resists radiotherapy, making it one of the deadliest forms of cancer. Here, we show that human lung cancer cell lines can be rendered sensitive to ionizing radiation (IR) by RNAi knockdown of C-terminus of Hsc70-interacting protein (CHIP/STUB1), a U-box-type E3 ubiquitin ligase that targets a number of stress-induced...Read More
Zhang B Hong LJ Duan GC Liang WJ Yang HY Xi YL
Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi 2017-02-01
Objective: To explore the stability of resistant phenotypes and changes of clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) gene system on four Shigella strains in the absence of antibiotics. Methods: Four clinical isolated Shigella strains that resistant to different antibiotics were co...Read More
Burkard Christine Lillico SimonG Reid Elizabeth Jackson Ben Mileham AlanJ Ait-Ali Tahar Whitelaw CBruceA Archibald AlanL
PLoS Pathogens 2017-02-01
Porcine Reproductive and Respiratory Syndrome (PRRS) is a panzootic infectious disease of pigs, causing major economic losses to the world-wide pig industry. PRRS manifests differently in pigs of all ages but primarily causes late-term abortions and stillbirths in sows and respiratory disease in piglets. The causative agent of t...Read More
Ipsaro JonathanJ Shen Chen Arai Eri Xu Yali Kinney JustinB Joshua-Tor Leemor Vakoc ChristopherR Shi Junwei
PLoS ONE 2017-02-01
Genetic alterations conferring resistance to the effects of chemical inhibitors are valuable tools for validating on-target effects in cells. Unfortunately, for many therapeutic targets such alleles are not available. To address this issue, we evaluated whether CRISPR-Cas9-mediated insertion/deletion (indel) mutagenesis can prod...Read More
Williams BartO Warman MatthewL
Journal of Bone and Mineral Research 2017-02-01
The Clustered Regularly Interspaced Palindromic Repeats (CRISPR)/CRISPR-associated protein (Cas) pathway is revolutionizing biological research. Modifications to this primitive prokaryotic immune system now enable scientists to efficiently edit DNA or modulate gene expression in living eukaryotic cells and organisms. Thus, many ...Read More
Guedn Anabel Swieboda Dawid Charles Mark Toussaint Marie Johnston SebastianL Asfor Amin Panjwani Anusha Tuthill TobiasJ Danahay Henry Raynham Tony Mousnier Aurelie Solari Roberto
Journal of Virology 2017-02-01
Picornavirus replication is known to cause extensive remodeling of Golgi and endoplasmic reticulum membranes, and a number of the host proteins involved in the viral replication complex have been identified, including oxysterol binding protein (OSBP) and phosphatidylinositol 4-kinase III beta (PI4KB). Since both OSBP and PI4KB a...Read More
Wen Yahong Liao Grace Pritchard Thomas Zhao Ting-Ting Connelly JonP Pruett-Miller ShondraM Blanc Valerie Davidson NicholasO Madison BlairB
Journal of Biological Chemistry 2017-02-01
The discovery and application of CRISPR/Cas9 technology for genome editing has greatly accelerated targeted mutagenesis in a variety of organisms. CRISPR/Cas9-mediated site-specific cleavage is typically exploited for the generation of insertions or deletions (indels) after aberrant dsDNA repair via the endogenous non-homology e...Read More
Eyquem Justin Mansilla-Soto Jorge Giavridis Theodoros vanderStegen SjoukjeJC Hamieh Mohamad Cunanan KristenM Odak Ashlesha Gnen Mithat Sadelain Michel
Nature 2017-02-01
Chimeric antigen receptors (CARs) are synthetic receptors that redirect and reprogram T cells to mediate tumour rejection. The most successful CARs used to date are those targeting CD19 (ref. 2), which offer the prospect of complete remission in patients with chemorefractory or relapsed B-cell malignancies. CARs are typically tr...Read More
Senturk Serif Shirole NitinH Nowak DawidG Corbo Vincenzo Pal Debjani Vaughan Alexander Tuveson DavidA Trotman LloydC Kinney JustinB Sordella Raffaella
Nature Communications 2017-02-01
The CRISPR/Cas9 system is a powerful tool for studying gene function. Here, we describe a method that allows temporal control of CRISPR/Cas9 activity based on conditional Cas9 destabilization. We demonstrate that fusing an FKBP12-derived destabilizing domain to Cas9 (DD-Cas9) enables conditional Cas9 expression and temporal cont...Read More
Liu Yun Zhu Zeyao Ho IdyHT Shi Yujian Xie Yuxin Li Jianzhen Zhang Yong Chan MatthewTV Cheng ChristopherHK
Experientia. Basel 2017-02-01
Zebrafish is an important model to study developmental biology and human diseases. However, an effective approach to achieve spatial and temporal gene knockout in zebrafish has not been well established. In this study, we have developed a new approach, namely bacterial artificial chromosome-rescue-based knockout (BACK), to achie...Read More
Borchardt ErinK Meganck RitaM Vincent HeatherA Ball ChristopherB Ramos SilviaBV Moorman NathanielJ Marzluff WilliamF Asokan Aravind
RNA 2017-02-01
Circular RNAs (circRNAs) are highly stable, covalently closed RNAs that are regulated in a spatiotemporal manner and whose functions are largely unknown. These molecules have the potential to be incorporated into engineered systems with broad technological implications. Here we describe a switch for inducing back-splicing of an ...Read More
Kim Eunji Koo Taeyoung Park SungWook Kim Daesik Kim Kyoungmi Cho Hee-Yeon Song DongWoo Lee KyuJun Jung MinHee Kim Seokjoong Kim JinHyoung Kim JeongHun Kim Jin-Soo
Nature Communications 2017-02-01
Several CRISPR-Cas9 orthologues have been used for genome editing. Here, we present the smallest Cas9 orthologue characterized to date, derived from Campylobacter jejuni (CjCas9), for efficient genome editing in vivo. After determining protospacer-adjacent motif (PAM) sequences and optimizing single-guide RNA (sgRNA) length, we ...Read More
Yang Hui Patel DinshawJ
Cell Research 2017-02-01
In bacteria and archaea, CRISPR-Cas adaptive immune systems utilize RNA-guided endonucleases to defend against invasion by foreign nucleic acids of bacteriophage, virus and plasmid origin. In a recent paper published in Nature, Burstein et al. identified the first Cas9 protein in uncultivated archaea and two novel CRISPR-CasX an...Read More
Chadwick AlexandraC Musunuru Kiran
Current Cardiology Reports 2017-02-01
The opportunities afforded through the recent advent of genome-editing technologies have allowed investigators to more easily study a number of diseases. The advantages and limitations of the most prominent genome-editing technologies are described in this review, along with potential applications specifically focused on cardiov...Read More
Budak Hikmet Zhang Baohong
Functional & Integrative Genomics 2017-02-01
Non-coding RNAs such as microRNAs (miRNAs) are very tiny ribonucleotides having an essential role in gene regulation at both post-transcriptional and translational levels. They are very conserved and expressed in worms, flies, plants, and mammals in a sequence-specific manner. Furthermore, it is now possible to clone miRNAs usin...Read More
Zhang Jian-Ping Li Xiao-Lan Li Guo-Hua Chen Wanqiu Arakaki Cameron Botimer GaryD Baylink David Zhang Lu Wen Wei Fu Ya-Wen Xu Jing Chun Noah Yuan Weiping Cheng Tao Zhang Xiao-Bing
Genome Biology 2017-02-01
Precise genome editing via homology-directed repair (HDR) after double-stranded DNA (dsDNA) cleavage facilitates functional genomic research and holds promise for gene therapy. However, HDR efficiency remains low in some cell types, including some of great research and clinical interest, such as human induced pluripotent stem ce...Read More
Abdelrahman Hisham ElHady Mohamed Alcivar-Warren Acacia Allen Standish Al-Tobasei Rafet Bao Lisui Beck Ben Blackburn Harvey Bosworth Brian Buchanan John Chappell Jesse Daniels William Dong Sheng Dunham Rex Durland Evan Elaswad Ahmed Gomez-Chiarri Marta Gosh Kamal Guo Ximing Hackett Perry Hanson Terry Hedgecock Dennis Howard Tiffany Holland Leigh Jackson Molly Jin Yulin Kahlil Karim Kocher Thomas Leeds Tim Li Ning Lindsey Lauren Liu Shikai Liu Zhanjiang Martin Kyle Novriadi Romi Odin Ramjie Palti Yniv Peatman Eric Proestou Dina Qin Guyu Reading Benjamin Rexroad Caird Roberts Steven Salem Mohamed Severin Andrew Shi Huitong Shoemaker Craig Stiles Sheila Tan Suxu Tang KathyFJ Thongda Wilawan Tiersch Terrence Tomasso Joseph Prabowo WendyTri Vallejo Roger vanderSteen Hein Vo Khoi Waldbieser Geoff Wang Hanping Wang Xiaozhu Xiang Jianhai Yang Yujia Yant Roger Yuan Zihao Zeng Qifan Zhou Tao
BMC Genomics 2017-02-01
Advancing the production efficiency and profitability of aquaculture is dependent upon the ability to utilize a diverse array of genetic resources. The ultimate goals of aquaculture genomics, genetics and breeding research are to enhance aquaculture production efficiency, sustainability, product quality, and profitability in sup...Read More
Chen Weizhong Zhang Yifei Yeo Won-Sik Bae Taeok Ji Quanjiang
Journal of the American Chemical Society 2017-02-01
Staphylococcus aureus, a major human pathogen, has been the cause of serious infectious diseases with a high mortality rate. Although genetics is a key means to study S. aureus physiology, such as drug resistance and pathogenesis, genetic manipulation in S. aureus is always time-consuming and labor-intensive. Here we report a CR...Read More
Lu Jian Shao Yanjiao Qin Xuan Liu Daozhi Chen Ang Li Dali Liu Mingyao Wang Xin
Scientific reports 2017-02-01
Cytochrome P450 (CYP) 3A accounts for nearly 30% of the total CYP enzymes in the human liver and participates in the metabolism of over 50% of clinical drugs. Moreover, CYP3A plays an important role in chemical metabolism, toxicity, and carcinogenicity. New animal models are needed to investigate CYP3A functions, especially for ...Read More
Williams TimothyJ Liao Yan Ye Jun Kuchel RhiannonP Poljak Anne Raftery MarkJ Cavicchioli Ricardo
Environmental Microbiology 2017-02-01
Halohasta litchfieldiae represents ? 44% and Halorubrum lacusprofundi ? 10% of the hypersaline, perennially cold (? -20C) Deep Lake community in Antarctica. We used proteomics and microscopy to define physiological responses of these haloarchaea to growth at high (30C) and low (10 and 4C) temperatures. The proteomic data indicat...Read More
Fei Fei Sun Shao-Yang Yao Yu-Xiao Wang Xu
Sheng li xue bao : [Acta physiologica Sinica] 2017-02-01
Obesity has become a severe public health problem across the world, and seriously affects the health and life quality of human beings. Here we generated lepr and mc4r mutant zebrafish via the CRISPR/Cas9 technique, and performed morphological and functional characterizations of those mutants. We observed that there was no signif...Read More
Lv Ji-Neng Zhou Gao-Hui Chen Xuejiao Chen Hui Wu Kun-Chao Xiang Lue Lei Xin-Lan Zhang Xiao Wu Rong-Han Jin Zi-Bing
Scientific reports 2017-02-01
Precursor messenger RNA (Pre-mRNA) splicing is an essential biological process in eukaryotic cells. Genetic mutations in many spliceosome genes confer human eye diseases. Mutations in the pre-mRNA splicing factor, RP9 (also known as PAP1), predispose autosomal dominant retinitis pigmentosa (adRP) with an early onset and severe v...Read More
McKinley KaraL Cheeseman IainM
Developmental Cell 2017-02-01
Defining the genes that are essential for cellular proliferation is critical for understanding organismal development and identifying high-value targets for disease therapies. However, the requirements for cell-cycle progression in human cells remain incompletely understood. To elucidate the consequences of acute and chronic eli...Read More
Zhang Yu Schmid Benjamin Nikolaisen NanettK Rasmussen MikkelA Aldana BlancaI Agger Mikkel Calloe Kirstine Stummann TinaC Larsen HjalteM Nielsen TroelsT Huang Jinrong Xu Fengping Liu Xin Bolund Lars Meyer Morten Bak LasseK Waagepetersen HelleS Luo Yonglun Nielsen JrgenE Holst Bjrn Clausen Christian Hyttel Poul Freude KristineK
Stem cell reports 2017-02-01
The truncated mutant form of the charged multivesicular body protein 2B (CHMP2B) is causative for frontotemporal dementia linked to chromosome 3 (FTD3). CHMP2B is a constituent of the endosomal sorting complex required for transport (ESCRT) and, when mutated, disrupts endosome-to-lysosome trafficking and substrate degradation. T...Read More
Liang Liya Liu Rongming Garst AndrewD Lee Thomas Nogu VioletaSnchezI Beckham GreggT Gill RyanT
Metabolic Engineering 2017-02-01
Isopropanol is an important target molecule for sustainable production of fuels and chemicals. Increases in DNA synthesis and synthetic biology capabilities have resulted in the development of a range of new strategies for the more rapid design, construction, and testing of production strains. Here, we report on the use of such ...Read More
Arendt Philipp Miettinen Karel Pollier Jacob DeRycke Riet Callewaert Nico Goossens Alain
Metabolic Engineering 2017-02-01
Saponins are a structurally diverse family of triterpenes that are widely found as main constituents in many traditional plant-based medicines and often have bioactivities of industrial interest. The heterologous production of triterpene saponins in microbes remains challenging and only limited successful pathway engineering end...Read More
Kotini AndrianaG Chang Chan-Jung Chow Arthur Yuan Han Ho Tzu-Chieh Wang Tiansu Vora Shailee Solovyov Alexander Husser Chrystel Olszewska Malgorzata Teruya-Feldstein Julie Perumal Deepak Klimek VirginiaM Spyridonidis Alexandros Rampal RaajitK Silverman Lewis Reddy EPremkumar Papaemmanuil Elli Parekh Samir Greenbaum BenjaminD Leslie ChristinaS Kharas MichaelG Papapetrou EiriniP
Cell Stem Cell 2017-02-01
Myeloid malignancy is increasingly viewed as a disease spectrum, comprising hematopoietic disorders that extend across a phenotypic continuum ranging from clonal hematopoiesis to myelodysplastic syndrome (MDS) and acute myeloid leukemia (AML). In this study, we derived a collection of induced pluripotent stem cell (iPSC) lines c...Read More
Burgess MichaelR Hwang Eugene Mroue Rana Bielski CraigM Wandler AnicaM Huang BenjaminJ Firestone AriJ Young Amy Lacap JenniferA Crocker Lisa Asthana Saurabh Davis ElizabethM Xu Jin Akagi Keiko LeBeau MichelleM Li Qing Haley Benjamin Stokoe David Sampath Deepak Taylor BarryS Evangelista Marie Shannon Kevin
Cell 2017-02-01
Investigating therapeutic outliers that show exceptional responses to anti-cancer treatment can uncover biomarkers of drug sensitivity. We performed preclinical trials investigating primary murine acute myeloid leukemias (AMLs) generated by retroviral insertional mutagenesis in Kras(G12D) knockin mice with the MEK inhibitor PD03...Read More
Riba Andrea Emmenlauer Mario Chen Amy Sigoillot Frederic Cong Feng Dehio Christoph Jenkins Jeremy Zavolan Mihaela
Cell Systems 2017-02-01
RNAi is broadly used to map gene regulatory networks, but the identification of genes that are responsible for the observed phenotypes is challenging, assmall interfering RNAs (siRNAs) simultaneously downregulate the intended on targets and many partially complementary off targets. Additionally, the scarcity of publicly availabl...Read More
Lim JaeSeok Gopalappa Ramu Kim SeHoon Ramakrishna Suresh Lee Minji Kim Woo-Il Kim Junho Park SangMin Lee Junehawk Oh Jung-Hwa Kim HeungDong Park Chang-Hwan Lee JoonSoo Kim Sangwoo Kim DongSeok Han JungMin Kang Hoon-Chul Kim HyongbumHenry Lee JeongHo
The American Journal of Human Genetics 2017-02-01
Focal cortical dysplasia (FCD) is a major cause of the sporadic form of intractable focal epilepsies that require surgical treatment. It has recently been reported that brain somatic mutations in MTOR account for 15%-25% of FCD type II (FCDII), characterized by cortical dyslamination and dysmorphic neurons. However, the genetic ...Read More
Kang YooKyung Kwon Kyu Ryu JeaSung Lee HaNeul Park Chankyu Chung HyunJung
Bioconjugate Chemistry 2017-02-01
The overuse of antibiotics plays a major role in the emergence and spread of multidrug-resistant bacteria. A molecularly targeted, specific treatment method for bacterial pathogens can prevent this problem by reducing the selective pressure during microbial growth. Herein, we introduce a nonviral treatment strategy delivering ge...Read More
Xiong Xiong Zhang Yanxiao Yan Jian Jain Surbhi Chee Sora Ren Bing Zhao Huimin
ACS Synthetic Biology 2017-02-01
Eukaryotic transcriptional factors (TFs) typically recognize short genomic sequences alone or together with other proteins to modulate gene expression. Mapping of TF-DNA interactions in the genome is crucial for understanding the gene regulatory programs in cells. While chromatin immunoprecipitation followed by sequencing (ChIP-...Read More
Soutourina Olga
Current Opinion in Microbiology 2017-02-01
Clostridium difficile (CD)-associated diarrhoea is currently the most prevalent nosocomial diarrhoea worldwide. Many characteristics of CD pathogenicity remain poorly understood. Recent data strongly indicate the importance of an RNA network for the control of gene expression in CD. More than 200 regulatory RNAs have been identi...Read More
Wu Yun Xu Kun Ren Chonghua Li Xinyi Lv Huijiao Han Furong Wei Zehui Wang Xin Zhang Zhiying
FEBS Letters 2017-02-01
The CRISPR/Cas9 system has recently emerged as a simple, yet powerful genome engineering tool, which has been widely used for genome modification in various organisms and cell types. However, screening biallelic genome modified cells are often time-consuming and technically challenging. In this study, we incorporated two differe...Read More
Devost Dominic Sleno Rory Petrin Darlaine Zhang Alice Shinjo Yuji Okde Rakan Aoki Junken Inoue Asuka Hebert TerenceE
Journal of Biological Chemistry 2017-02-01
Here, we report the design and use of GPCR-based biosensors to monitor ligand-mediated conformational changes in receptors in intact cells. These biosensors use Bioluminescence Resonance Energy Transfer (BRET) with Renilla luciferase (RlucII) as an energy donor, placed at the distal end of the receptor C-tail and the small fluor...Read More
Deaner Matthew Alper HalS
Metabolic Engineering 2017-02-01
Dissecting genotype-phenotype relationships in a high-throughput and scalable manner is still an unresolved problem facing metabolic engineers. While the RNA-guided nuclease Cas9 has been repurposed as a programmable transcription regulator, its application has typically been limited to binary on/off regulation and thus misses i...Read More
Barrangou Rodolphe Gersbach CharlesA
Molecular Cell 2017-02-01
In this issue of Molecular Cell, Smargon etal. (2017) unearth Cas13b from type VI-B CRISPR-Cas immune systems and characterize its RNA-guided, RNA-targeting activity, including regulation by the novel co-factors Csx27 and Csx28, as well as non-specific collateral RNA damage.Read More
Yao Xiaoming Verkman AlanS
Acta neuropathologica communications 2017-02-01
Neuromyelitis optica spectrum disorders (herein called NMO) is an inflammatory demyelinating disease of the central nervous system in which pathogenesis involves complement-dependent cytotoxicity (CDC) produced by immunoglobulin G autoantibodies targeting aquaporin-4 (AQP4-IgG) on astrocytes. We reported evidence previously, usi...Read More
Garca-Tun Ignacio Hernndez-Snchez Mara Ordoez JosLuis Alonso-Prez Veronica lamo-Quijada Miguel Benito Rocio Guerrero Carmen Hernndez-Rivas JessMara Snchez-Martn Manuel
Oncotarget 2017-02-01
CRISPR/Cas9 technology was used to abrogate p210 oncoprotein expression in the Boff-p210 cell line, a pro-B line derived from interlukin-3-dependent Baf/3, that shows IL-3-independence arising from the constitutive expression of BCR-ABL p210. Using this approach, pools of Boff-p210-edited cells and single edited cell-derived clo...Read More
Axton RichardA Haideri SharminS Lopez-Yrigoyen Martha Taylor HelenA Forrester LesleyM
PLoS ONE 2017-02-01
Engineered nucleases have been used to generate knockout or reporter cell lines and a range of animal models for human disease. These new technologies also hold great promise for therapeutic genome editing. Current methods to evaluate the activity of these nucleases are time consuming, require extensive optimization and are hamp...Read More
Tang Xu Lowder LeviG Zhang Tao Malzahn AimeeA Zheng Xuelian Voytas DanielF Zhong Zhaohui Chen Yiyi Ren Qiurong Li Qian Kirkland ElidaR Zhang Yong Qi Yiping
Nature Plants 2017-02-01
Clustered regularly interspaced short palindromic repeats (CRISPR)-Cpf1 has emerged as an effective genome editing tool in animals. Here we compare the activity of Cpf1 from Acidaminococcus sp. BV3L6 (As) and Lachnospiraceae bacterium ND2006 (Lb) in plants, using a dual RNA polymerase II promoter expression system. LbCpf1 genera...Read More
Bian Shengtai Zhou Yicen Hu Yawei Cheng Jing Chen Xiaofang Xu Youchun Liu Peng
Scientific reports 2017-02-01
Arrayed genetic screens mediated by the CRISPR/Cas9 technology with single guide RNA (sgRNA) libraries demand a high-throughput platform capable of transfecting diverse cell types at a high efficiency in a genome-wide scale for detection and analysis of sophisticated cellular phenotypes. Here we developed a high-throughput in si...Read More
Trudel Luc Frenette Michel Moineau Sylvain
Nature Microbiology 2017-02-01
Khalili Kamel White MartynK Jacobson JeffreyM
Experientia. Basel 2017-02-01
HIV/AIDS remains a major public health issue. In 2014, it was estimated that 36.9million people are living with HIV worldwide, including 2.6million children. Since the advent of combination antiretroviral therapy (cART), in the 1990s, treatment has been so successful that in many parts of the world, HIV has become a chronic cond...Read More
Borgo Christian Franchin Cinzia Scalco Stefano Bosello-Travain Valentina Donella-Deana Arianna Arrigoni Giorgio Salvi Mauro Pinna LorenzoA
Scientific reports 2017-02-01
CK2 is a ubiquitous, constitutively active, highly pleiotropic, acidophilic Ser/Thr protein kinase whose holoenzyme is composed of two catalytic (? and/or ?') subunits and a dimer of a non-catalytic ? subunit. Abnormally high CK2 level/activity is often associated with malignancy and a variety of cancer cells have been shown to ...Read More
Raveux Aurlien Vandormael-Pournin Sandrine Cohen-Tannoudji Michel
Scientific reports 2017-02-01
Microinjection of the CRISPR/Cas9 system in zygotes is an efficient and comparatively fast method to generate genetically modified mice. So far, only few knock-in mice have been generated using this approach, and because no systematic study has been performed, parameters controlling the efficacy of CRISPR/Cas9-mediated targeted ...Read More
Contreras JorgeL Sherkow JacobS
Science 2017-02-01
Haemmig Stefan Feinberg MarkW
Circulation Research 2017-02-01
Kim Kyoungmi Park SungWook Kim JinHyoung Lee SeungHwan Kim Daesik Koo Taeyoung Kim Kwang-Eun Kim JeongHun Kim Jin-Soo
Genome Research 2017-02-01
RNA-guided genome surgery using CRISPR-Cas9 nucleases has shown promise for the treatment of diverse genetic diseases. Yet, the potential of such nucleases for therapeutic applications in nongenetic diseases is largely unexplored. Here, we focus on age-related macular degeneration (AMD), a leading cause of blindness in adults, w...Read More
Bence Melinda Jankovics Ferenc Lukcsovich Tams Erdlyi Mikls
FEBS Journal 2017-02-01
Inducible protein degradation techniques have considerable advantages over classical genetic approaches, which generate loss-of-function phenotypes at the gene or mRNA level. The plant-derived auxin-inducible degradation system (AID) is a promising technique which enables the degradation of target proteins tagged with the AID mo...Read More
Hynes AlexanderP Lemay Marie-Laurence Trudel Luc Deveau Hlne Frenette Michel Tremblay DeniseM Moineau Sylvain
Nature Protocols 2017-02-01
CRISPR (clustered regularly interspaced short palindromic repeats)-Cas systems have been adapted into a powerful genome-editing tool. The basis for the flexibility of the tool lies in the adaptive nature of CRISPR-Cas as a bacterial immune system. Here, we describe a protocol to experimentally demonstrate the adaptive nature of ...Read More
Lonowski LindseyA Narimatsu Yoshiki Riaz Anjum Delay CatherineE Yang Zhang Niola Francesco Duda Katarzyna Ober ElkeA Clausen Henrik Wandall HansH Hansen SteenH Bennett EricP Frdin Morten
Nature Protocols 2017-02-01
This protocol describes methods for increasing and evaluating the efficiency of genome editing based on the CRISPR-Cas9 (clustered regularly interspaced short palindromic repeats-CRISPR-associated 9) system, transcription activator-like effector nucleases (TALENs) or zinc-finger nucleases (ZFNs). First, Indel Detection by Amplic...Read More
Estoppey David Hewett JeffreyW Guy ChantaleT Harrington Edmund Thomas JasonR Schirle Markus Cuttat Rachel Waldt Annick Gerrits Bertran Yang Zinger Schuierer Sven Pan Xuewen Xie Kevin Carbone Walter Knehr Judith Lindeman Alicia Russ Carsten Frias Elizabeth Hoffman GregoryR Varadarajan Malini Ramadan Nadire Reece-Hoyes JohnS Wang Qiong Chen Xin McAllister Gregory Roma Guglielmo Bouwmeester Tewis Hoepfner Dominic
Scientific reports 2017-02-01
Chemogenomic profiling is a powerful and unbiased approach to elucidate pharmacological targets and the mechanism of bioactive compounds. Until recently, genome-wide, high-resolution experiments of this nature have been limited to fungal systems due to lack of mammalian genome-wide deletion collections. With the example of a nov...Read More
Walters BrandonJ Mercaldo Valentina Gillon ColleenJ Yip Matthew Neve RachaelL Boyce FrederickM Frankland PaulW Josselyn SheenaA
The formation of long-lasting memories requires coordinated changes in gene expression and protein synthesis (Davis and Squire, 1984; Duvarci et al, 2008; Hernandez and Abel, 2008). Although many studies implicate DNA modifications (DNA methylation, histone modifications) in memory formation, the contributions of RNA modificatio...Read More
Kim Hyeran Kim Sang-Tae Ryu Jahee Kang Beum-Chang Kim Jin-Soo Kim Sang-Gyu
Nature Communications 2017-02-01
Cpf1, a type V CRISPR effector, recognizes a thymidine-rich protospacer-adjacent motif and induces cohesive double-stranded breaks at the target site guided by a single CRISPR RNA (crRNA). Here we show that Cpf1 can be used as a tool for DNA-free editing of plant genomes. We describe the delivery of recombinant Cpf1 proteins wit...Read More
Musharova Olga Klimuk Evgeny Datsenko KirillA Metlitskaya Anastasia Logacheva Maria Semenova Ekaterina Severinov Konstantin Savitskaya Ekaterina
Nucleic Acids Research 2017-02-01
Yan Jifang Chuai Guohui Zhou Chi Zhu Chenyu Yang Jing Zhang Chao Gu Feng Xu Han Wei Jia Liu Qi
Briefings in Bioinformatics 2017-02-01
Gibson GaryJ Yang Maozhou
Nature Reviews: Rheumatology 2017-02-01
CRISPR/Cas9 genome editing technology has taken the research world by storm since its use in eukaryotes was first proposed in 2012. Publications describing advances in technology and new applications have continued at an unrelenting pace since that time. In this Review, we discuss the application of CRISPR/Cas9 for creating gene...Read More
Shcherbakova Aleksandra Tiemann Birgit Buettner FalkFR Bakker Hans
PNAS 2017-02-01
Thrombospondin type 1 repeats (TSRs) occur in diverse proteins involved in adhesion and signaling. The two extracellular TSRs of the netrin receptor UNC5A contain WxxWxxWxxC motifs that can be C-mannosylated on all tryptophans. A single C-mannosyltransferase (dumpy-19, DPY-19), modifying the first two tryptophans, occurs in Caen...Read More
Walter DavidM Venancio OliviaS Buza ElizabethL Tobias JohnW Deshpande Charuhas Gudiel AAndrea Kim-Kiselak Caroline Cicchini Michelle Yates TravisJ Feldser DavidM
Cancer Research 2017-02-01
Chromatin modifying genes are frequently mutated in human lung adenocarcinoma, but the functional impact of these mutations on disease initiation and progression is not well understood. Using a CRISPR-based approach, we systematically inactivated three of the most commonly mutated chromatin regulatory genes in two KrasG12D-drive...Read More
Nie Jing Hashino Eri
EMBO Reports 2017-02-01
Three-dimensional (3D) stem cell differentiation cultures recently emerged as a novel model system for investigating human embryonic development and disease progression in vitro, complementing existing animal and two-dimensional (2D) cell culture models. Organoids, the 3D self-organizing structures derived from pluripotent or so...Read More
Freidlin PaulJeffrey Nissan Israel Luria Anna Goldblatt Drora Schaffer Lana Kaidar-Shwartz Hasia Chemtob Daniel Dveyrin Zeev Head StevenRobert Rorman Efrat
BMC Genomics 2017-02-01
CRISPR and CRISPR-flanking genomic regions are important for molecular epidemiology of Mycobacterium tuberculosis complex (MTBC) strains, and potentially for adaptive immunity to phage and plasmid DNA, and endogenous roles in the bacterium. Genotyping in the Israel National Mycobacterium Reference Center Tel-Aviv of over 1500 MT...Read More
Ren Jiangtao Zhang Xuhua Liu Xiaojun Fang Chongyun Jiang Shuguang June CarlH Zhao Yangbing
Oncotarget 2017-02-01
The therapeutic potential of CRISPR system has already been demonstrated in many instances and begun to overlap with the rapidly expanding field of cancer immunotherapy, especially on the production of genetically modified T cell receptor or chimeric antigen receptor (CAR) T cells. Efficient genomic disruption of multiple gene l...Read More
Gong Haixia Liu Menglin Klomp Jeff Merrill BradleyJ Rehman Jalees Malik AsrarB
Scientific reports 2017-02-01
Human endothelial cells (ECs) are widely used to study mechanisms of angiogenesis, inflammation, and endothelial permeability. Targeted gene disruption induced by Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-CRISPR-Associated Protein 9 (Cas9) nuclease gene editing is potentially an important tool for defini...Read More
Moreno AnaM Mali Prashant
Wiley Interdisciplinary Reviews: Systems Biology and Medicine 2017-02-01
Differences in genomes underlie most organismal diversity, and aberrations in genomes underlie many disease states. With the growing knowledge of the genetic and pathogenic basis of human disease, development of safe and efficient platforms for genome and epigenome engineering will transform our ability to therapeutically target...Read More
Gorski StanislawA Vogel Jrg Doudna JenniferA
Nature Reviews: Molecular Cell Biology 2017-02-01
RNA is involved in the regulation of multiple cellular processes, often by forming sequence-specific base pairs with cellular RNA or DNA targets that must be identified among the large number of nucleic acids in a cell. Several RNA-based regulatory systems in eukaryotes, bacteria and archaea, including microRNAs (miRNAs), small ...Read More
Sosnovtsev StanislavV Sandoval-Jaime Carlos Parra GabrielI Tin ChristineM Jones RonaldW Soden Jo Barnes Donna Freeth Jim Smith AlvinW Green KimY
mBio 2017-02-01
The Hom-1 vesivirus was reported in 1998 following the inadvertent transmission of the animal calicivirus San Miguel sea lion virus to a human host in a laboratory. We characterized the Hom-1 strain and investigated the mechanism by which human cells could be infected. An expression library of 3,559 human plasma membrane protein...Read More
Liu Yangyang Han Xiao Yuan Junting Geng Tuoyu Chen Shihao Hu Xuming Cui IsabelleH Cui Hengmi
Journal of Biological Chemistry 2017-02-01
The type II bacterial CRISPR/Cas9 system is a simple, convenient and powerful tool for gene editing that allows for gene targeting. Here, we describe a CRISPR/Cas9-based approach for silencing both protein-coding and non-protein-coding genes by biallelic integration of a PolyA signal. Because of the integration of a BGH PolyA si...Read More
Wang Bin Wang Yujie Frabutt DylanA Zhang Xihe Yao Xiaoyu Hu Dan Zhang Zhuo Liu Chaonan Zheng Shimin Xiang Shi-Hua Zheng Yong-Hui
Journal of Biological Chemistry 2017-02-01
The Ebola virus (EBOV) trimeric envelope glycoprotein (GP) precursors are cleaved into the receptor-binding GP1 and the fusion-mediating GP2 subunits and incorporated into virions to initiate infection. GP1 and GP2 form heterodimers, which have 15 or 2 N-glycosylation sites (NGSs), respectively. Here, we investigated the mechani...Read More
Shi Zhong-Dong Lee Kihyun Yang Dapeng Amin Sadaf Verma Nipun Li QingV Zhu Zengrong Soh Chew-Li Kumar Ritu Evans Todd Chen Shuibing Huangfu Danwei
Cell Stem Cell 2017-02-01
Human disease phenotypes associated with haploinsufficient gene requirements are often not recapitulated well in animal models. Here, we have investigated the association between human GATA6 haploinsufficiency and a wide range of clinical phenotypes that include neonatal and adult-onset diabetes using CRISPR (clustered regularly...Read More
Murakami Nagahisa Imamura Keiko Izumi Yuishin Egawa Naohiro Tsukita Kayoko Enami Takako Yamamoto Takuya Kawarai Toshitaka Kaji Ryuji Inoue Haruhisa
Molecular Brain 2017-02-01
Hereditary motor and sensory neuropathy with proximal dominant involvement (HMSN-P) is caused by a heterozygous mutation (P285L) in Tropomyosin-receptor kinase Fused Gene (TFG), histopathologically characterized by progressive spinal motor neuron loss with TFG cytosolic aggregates. Although the TFG protein, found as a type of fu...Read More
Bengtsson NiclasE Hall JohnK Odom GuyL Phelps MichaelP Andrus ColinR Hawkins RDavid Hauschka StephenD Chamberlain JoelR Chamberlain JeffreyS
Nature Communications 2017-02-01
Gene replacement therapies utilizing adeno-associated viral (AAV) vectors hold great promise for treating Duchenne muscular dystrophy (DMD). A related approach uses AAV vectors to edit specific regions of the DMD gene using CRISPR/Cas9. Here we develop multiple approaches for editing the mutation in dystrophic mdx(4cv) mice usin...Read More
Hirose Michiko Hasegawa Ayumi Mochida Keiji Matoba Shogo Hatanaka Yuki Inoue Kimiko Goto Tatsuhiko Kaneda Hideki Yamada Ikuko Furuse Tamio Abe Kuniya Uenoyama Yoshihisa Tsukamura Hiroko Wakana Shigeharu Honda Arata Ogura Atsuo
Scientific reports 2017-02-01
Wild-derived mice have contributed to experimental mouse genetics by virtue of their genetic diversity, which may help increase the chance of identifying novel modifier genes responsible for specific phenotypes and diseases. However, gene targeting using wild-derived mice has been unsuccessful because of the unavailability of st...Read More
Park Ki-Eun Powell Anne Sandmaier ShelleyES Kim Chan-Mi Mileham Alan Donovan DavidM Telugu BhanuP
Scientific reports 2017-02-01
The domestic pig is an important dual purpose animal model for agricultural and biomedical applications. There is an emerging consensus in the biomedical community for the use of large animal models such as pigs to either serve as an alternative, or complement investigations from the mouse. However, the use of pig has not proven...Read More
Seong Hyun-A Manoharan Ravi Ha Hyunjung
Scientific reports 2017-02-01
Murine protein serine-threonine kinase 38 (MPK38), an AMP-activated protein kinase (AMPK)-related kinase, has been implicated in the induction of apoptosis signal-regulating kinase 1 (ASK1)-, transforming growth factor-? (TGF-?)-, and p53-mediated activity involved in metabolic homeostasis. Here, zinc finger protein ZPR9 was fou...Read More
Zhang Linlin Martin Arnaud Perry MichaelW vanderBurg KarinRL Matsuoka Yuji Monteiro Antnia Reed RobertD
Genetics 2017-02-01
Despite the variety, prominence, and adaptive significance of butterfly wing patterns surprisingly little known about the genetic basis of wing color diversity. Even though there is intense interest in wing pattern evolution and development, the technical challenge of genetically manipulating butterflies has slowed efforts to fu...Read More
Smith JustinD Schlecht Ulrich Xu Weihong Suresh Sundari Horecka Joe Proctor MichaelJ Aiyar RaekaS Bennett RichardAO Chu Angela Li YongFuga Roy Kevin Davis RonaldW Steinmetz LarsM Hyman RichardW Levy SashaF StOnge RobertP
Molecular Systems Biology 2017-02-01
The low costs of array-synthesized oligonucleotide libraries are empowering rapid advances in quantitative and synthetic biology. However, high synthesis error rates, uneven representation, and lack of access to individual oligonucleotides limit the true potential of these libraries. We have developed a cost-effective method cal...Read More
Chan LaiN Chen Zhengshan Braas Daniel Lee Jae-Woong Xiao Gang Geng Huimin Cosgun KadriyeNehir Hurtz Christian Shojaee Seyedmehdi Cazzaniga Valeria Schjerven Hilde Ernst Thomas Hochhaus Andreas Kornblau StevenM Konopleva Marina Pufall MilesA Cazzaniga Giovanni Liu GraceJ Milne ThomasA Koeffler HPhillip Ross TheodoraS Snchez-Garca Isidro Borkhardt Arndt Yamamoto KeithR Dickins RossA Graeber ThomasG Mschen Markus
Nature 2017-02-01
B-lymphoid transcription factors, such as PAX5 and IKZF1, are critical for early B-cell development, yet lesions of the genes encoding these transcription factors occur in over 80% of cases of pre-B-cell acute lymphoblastic leukaemia (ALL). The importance of these lesions in ALL has, until now, remained unclear. Here, by combini...Read More
Lau HY Tang J Casey PJ Wang M
Oncogene 2017-02-01
Despite extensive effort, there has been limited progress in the development of direct RAS inhibitors. Targeting isoprenylcysteine carboxylmethyltransferase (ICMT), a unique enzyme of RAS post-translational modification, represents a promising strategy to inhibit RAS function. However, there lacks direct genetic evidence on the ...Read More
Ma Sanyuan Liu Yue Liu Yuanyuan Chang Jiasong Zhang Tong Wang Xiaogang Shi Run Lu Wei Xia Xiaojuan Zhao Ping Xia Qingyou
Insect Biochemistry and Molecular Biology 2017-02-01
Genome editing enabled unprecedented new opportunities for targeted genomic engineering of a wide variety of organisms ranging from microbes, plants, animals and even human embryos. The serial establishing and rapid applications of genome editing tools significantly accelerated Bombyx mori (B. mori) research during the past year...Read More
Spraggon Lee Martelotto LucianoG Hmeljak Julija Hitchman TylerD Wang Jiang Wang Lu Slotkin EmilyK Fan Pang-Dian Reis-Filho JorgeS Ladanyi Marc
Journal of Pathology 2017-02-01
Chromosomal rearrangements encoding oncogenic fusion proteins are found in a wide variety of malignancies. The use of programmable nucleases to generate specific double-strand breaks in endogenous loci, followed by non-homologous end joining DNA repair, has allowed several of these translocations to be generated as constitutivel...Read More
Canver MatthewC Bauer DanielE Dass Abhishek Yien YvetteY Chung Jacky Masuda Takeshi Maeda Takahiro Paw BarryH Orkin StuartH
Journal of Biological Chemistry 2017-02-01
Morizane Ryuji Bonventre JosephV
Trends in Molecular Medicine 2017-02-01
Human pluripotent stem cells (hPSCs) are attractive sources for regenerative medicine and disease modeling in vitro. Directed hPSC differentiation approaches have derived from knowledge of cell development in vivo rather than from stochastic cell differentiation. Moreover, there has been great success in the generation of 3D org...Read More