Custom DNA Oligos – Custom Formats

As automation continues to become a focus for many laboratories, delivery of oligonucleotide products in custom formats, such as 96 and 384 well-plates, presents more challenges to ensure consistent quality. Product format, such as specific plates, concentration and the mixing of forward and reverse primers necessitates detailed specifications. Additional quality control measures are in place to verify accuracy for custom formats. These measures may include:

  • Plate orientation and well position verification by mass spectrometry
  • Mixed Primers, i.e. Forward and Reverse in a single well, verified by mass spectroscopy, see image below
  • Verification for machine readable barcodes (1D and 2D)
  • Visual inspection of all master and daughter plates prior to shipment

Mixed Forward and Reverse Primers by Mass Spectrometry