HL-1 Cells Publications

W C Claycomb, N A Lanson, B S Stallworth, D B Egeland, J B Delcarpio, A Bahinski, N J Izzo
PNAS 1998-03-17
We have derived a cardiac muscle cell line, designated HL-1, from the AT-1 mouse atrial cardiomyocyte tumor lineage. HL-1 cells can be serially passaged, yet they maintain the ability to contract and retain differentiated cardiac morphological, biochemical, and electrophysiological properties. Ultrastructural characteristics typ...Read More
Leenaporn Jongpaiboonkit, William J King, Gary E Lyons, Amy L Paguirigan, Jay W Warrick, David J Beebe, William L Murphy
Biomaterials 2008-08-01
Hydrogels have been commonly used as model systems for 3-dimensional (3-D) cell biology, as they have material properties that resemble natural extracellular matrices (ECMs), and their cell-interactive properties can be readily adapted in order to address a particular hypothesis. Natural and synthetic hydrogels have been used to...Read More
Andrey V Kuznetsov, Sabzali Javadov, Stephan Sickinger, Sandra Frotschnig, Michael Grimm
Biochimica et Biophysica Acta 2015-02-01
Dysfunction of cardiac energy metabolism plays a critical role in many cardiac diseases, including heart failure, myocardial infarction and ischemia-reperfusion injury and organ transplantation. The characteristics of these diseases can be elucidated in vivo, though animal-free in vitro experiments, with primary adult or neonata...Read More
Ricardo Rodríguez-Calvo, Manuel Vázquez-Carrera, Luis Masana, Dietbert Neumann
PPAR Research 2015-01-01
Here we studied the impact of 5-aminoimidazole-4-carboxamide riboside (AICAR), a well-known AMPK activator, on cardiac metabolic adaptation. AMPK activation by AICAR was confirmed by increased phospho-Thr(172)-AMPK and phospho-Ser(79)-ACC protein levels in HL-1 cardiomyocytes. Then, cells were exposed to AICAR stimulation for 24...Read More
Li Wang, Shenghua Liu, Hongliang Zhang, Shengshou Hu, Yingjie Wei
International journal of clinical and experimental medicine 2015-01-01
Arrhythmogenic cardiomyopathy (AC) is a familial heart muscle disease with mutations of desmosomal gene and its pathogenesis is related with mutations of desmosomal gene and abnormality of connexin43 (Cx43). One of Rho GTPase, RhoA involves in many pathological processes and is regulated by desmosomal gene PKP2. We aim to identi...Read More
Yen-Yu Lu, Yao-Chang Chen, Yu-Hsun Kao, Yung-Kuo Lin, Yung-Hsin Yeh, Shih-Ann Chen, Yi-Jen Chen
Journal of Cellular and Molecular Medicine 2016-06-01
Colchicine is a microtubule disruptor that reduces the occurrence of atrial fibrillation (AF) after an operation or ablation. However, knowledge of the effects of colchicine on atrial myocytes is limited. The aim of this study was to determine if colchicine can regulate calcium (Ca(2+) ) homeostasis and attenuate the electrical ...Read More
Karin Åström-Olsson, Lizhen Li, Charlotta S. Olofsson, Jan Borén, Hans Öhlin, Lars Grip
Biochemical and Biophysical Research Communications 2012-06-15
Highlights ► HL-1 cells survival threshold is 6–12h at ischemia. ► Hypoxia alone did not influence HL-1 cells survival until >18h. ► FKBP12 and FKBP12.6 and calcium disturbances at ischemia and reperfusion.Read More
Nathalie Nguyen, Michael Biet, Elie Simard, Eric Béliveau, Nancy Francoeur, Gaétan Guillemette, Robert Dumaine, Michel Grandbois, Guylain Boulay
Biochimica et Biophysica Acta 2013-06-01
STIM1 plays a crucial role in Ca(2+) homeostasis, particularly in replenishing the intracellular Ca(2+) store following its depletion. In cardiomyocytes, the Ca(2+) content of the sarcoplasmic reticulum must be tightly controlled to sustain contractile activity. The presence of STIM1 in cardiomyocytes suggests that it may play a...Read More
Yu-Hsun Kao, Yao-Chang Chen, Yung-Kuo Lin, Rong-Jie Shiu, Tze-Fan Chao, Shih-Ann Chen, Yi-Jen Chen
European Journal of Clinical Investigation 2014-08-01
Fibroblast growth factor (FGF)-23 is a key regulator of phosphate homeostasis. Higher FGF-23 levels are correlated with poor outcomes in cardiovascular diseases. FGF-23 can produce cardiac hypertrophy and increase intracellular calcium, which can change cardiac electrical activity. However, it is not clear whether FGF-23 possess...Read More
Maura Brioschi, Sabrina Lento, Simona Barcella, Md Talat Nasim, Stefania Ghilardi, Silvia Stella Barbieri, Elena Tremoli, Cristina Banfi
Data in Brief 2015-06-01
This data article is related to the research article entitled Proteomics of Tissue Factor silencing in cardiomyocytic cells reveals a new role for this coagulation factor in splicing machinery control by Lento et al. [1]. Tissue Factor (TF) is a key player in the coagulation cascade, but it has additional functions ranging from ...Read More
F Rao, C Y Deng, Q H Zhang, Y M Xue, D Z Xiao, S J Kuang, Q X Lin, Z X Shan, X Y Liu, J N Zhu, X Y Yu, S L Wu
Brazilian Journal of Medical and Biological Research 2013-09-01
Macrophage migration inhibitory factor (MIF), a pleiotropic cytokine, plays an important role in the pathogenesis of atrial fibrillation; however, the upstream regulation of MIF in atrial myocytes remains unclear. In the present study, we investigated whether and how MIF is regulated in response to the renin-angiotensin system a...Read More
Zhenjiang Yang, Katherine T Murray
Journal of Cardiovascular Pharmacology 2011-01-01
Although normally absent, spontaneous pacemaker activity can develop in human atrium to promote tachyarrhythmias. HL-1 cells are immortalized atrial cardiomyocytes that contract spontaneously in culture, providing a model system of atrial cell automaticity. Using electrophysiologic recordings and selective pharmacologic blockers...Read More
Myriam Elschami, Michaela Scherr, Brigitte Philippens, Rita Gerardy-Schahn
European Journal of Cell Biology 2013-01-01
Signal transducer and activator of transcription 3 (STAT3) is an important mediator of cardiac survival pathways. Reduced levels of STAT3 in patients with end-stage heart failure suggest a clinical relevance of STAT3 deficiency for cardiac disease. The recent identification of STAT3 as a mitochondrial protein which is important ...Read More
Alexander Strigun, Fozia Noor, Alejandro Pironti, Jens Niklas, Tae Hoon Yang, Elmar Heinzle
Journal of Biotechnology 2011-09-20
Verapamil has been shown to inhibit glucose transport in several cell types. However, the consequences of this inhibition on central metabolism are not well known. In this study we focused on verapamil induced changes in metabolic fluxes in a murine atrial cell line (HL-1 cells). These cells were adapted to serum free conditions...Read More
Ofer Beharier, Shani Dror, Shiri Levy, Joy Kahn, Merav Mor, Sharon Etzion, Daniel Gitler, Amos Katz, Anthony J Muslin, Arie Moran, Yoram Etzion
Journal of Molecular Medicine (Berlin, Germany) 2012-02-01
Activation of ERK signaling may promote cardioprotection from ischemia-reperfusion (I/R) injury. ZnT-1, a protein that confers resistance from zinc toxicity, was found to interact with Raf-1 kinase through its C-terminal domain, leading to downstream activation of ERK. In the present study, we evaluated the effects of ZnT-1 in c...Read More
Y K Ting, K Morikawa, Y Kurata, P Li, U Bahrudin, E Mizuta, M Kato, J Miake, Y Yamamoto, A Yoshida, M Murata, T Inoue, A Nakai, G Shiota, K Higaki, E Nanba, H Ninomiya, Y Shirayoshi, I Hisatome
British Journal of Pharmacology 2011-04-01
The expression of voltage-dependent K(+) channels (K(v) ) 1.5 is regulated by members of the heat shock protein (Hsp) family. We examined whether the heat shock transcription factor 1 (HSF-1) and its inducer geranylgeranylacetone (GGA) could affect the expression of K(v) 1.5 channels and its anchoring protein, synapse associated...Read More
Rita Guzun, Minna Karu-Varikmaa, Marcela Gonzalez-Granillo, Andrey V Kuznetsov, Lauriane Michel, Cécile Cottet-Rousselle, Merle Saaremäe, Tuuli Kaambre, Madis Metsis, Michael Grimm, Charles Auffray, Valdur Saks
Biochimica et Biophysica Acta 2011-04-01
Mitochondria-cytoskeleton interactions were analyzed in adult rat cardiomyocytes and in cancerous non-beating HL-1 cells of cardiac phenotype. We show that in adult cardiomyocytes βII-tubulin is associated with mitochondrial outer membrane (MOM). βI-tubulin demonstrates diffused intracellular distribution, βIII-tubulin is coloca...Read More
M Xia, J J Salata, D J Figueroa, A-M Lawlor, H A Liang, Y Liu, T M Connolly
Journal of Molecular and Cellular Cardiology 2004-01-01
In the search for a readily available source of native cardiac cells, we investigated the molecular and pharmacological properties of the immortalized cardiac atrial myocyte cell line, HL-1 cells. This work focused on the expression pattern of voltage-gated Ca2+ channels (VGCC). Reverse transcription-polymerase chain reaction an...Read More
Anna Czeschik, Philipp Rinklin, Ulrike Derra, Sabrina Ullmann, Peter Holik, Siegfried Steltenkamp, Andreas Offenhäusser, Bernhard Wolfrum
Nanoscale 2015-01-01
Microelectrode arrays (MEAs) are state-of-the-art devices for extracellular recording and stimulation on biological tissue. Furthermore, they are a relevant tool for the development of biomedical applications like retina, cochlear and motor prostheses, cardiac pacemakers and drug screening. Hence, research on functional cell-sen...Read More
Peter Strege, Arthur Beyder, Cheryl Bernard, Ruben Crespo-Diaz, Atta Behfar, Andre Terzic, Michael Ackerman, Gianrico Farrugia
Channels 2012-01-01
Na(V)1.5 is a mechanosensitive voltage-gated Na(+) channel encoded by the gene SCN5A, expressed in cardiac myocytes and required for phase 0 of the cardiac action potential (AP). In the cardiomyocyte, ranolazine inhibits depolarizing Na(+) current and delayed rectifier (I(Kr)) currents. Recently, ranolazine was also shown to be ...Read More
Jun Zhang, Ji Youn Youn, Antony Y Kim, Rafael J Ramirez, Ling Gao, Doan Ngo, Peng Chen, Jennifer Scovotti, Aman Mahajan, Hua Cai
Frontiers in Physiology 2012-01-01
Atrial fibrillation (AF) is the most common type of cardiac arrhythmia with patients dying frequently of stroke. In view of the unclear etiologies of AF and a potential role of oxidative stress, the present study examined cardiac reactive oxygen species production and NADPH oxidase (NOX) expression in AF patients. Patients with ...Read More
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