Immobilon® PVDF membranes for Western blotting

We offer four different Immobilon® PVDF (Polyvinylidene Difluoride) membranes, each optimized for different protein blotting applications. We also now offer blotting sandwiches that feature pre-cut sheets of membrane and blotting filter paper.

  • Immobilon®-E membrane is the only PVDF membrane (0.45 µm) that wets out in aqueous buffers, eliminating the methanol pre-wet step. This membrane is well-suited for most Western blotting applications.
  • Immobilon®-P membranes are optimized for Western blotting and offer better handling and staining than nitrocellulose. All Immobilon-P PVDF membranes have a pore size of 0.45 μm.
  • Immobilon®-PSQ membrane (0.2 µm) is ideal for protein sequencing and immunoblotting of low molecular weight proteins. It has a higher protein binding capacity and a higher retention than 0.45 µm membranes.
  • Immobilon®-FL membrane (0.45 µm) was developed for fluorescence-based immunodetection. It has very low background fluorescence across a wide range of excitation and emission wavelengths.

Immobilon® PVDF Membrane Advantages Compared to Nitrocellulose

  • Won’t crack or curl, and they can be cut without fracturing.
  • Low background
  • Broad solvent compatibility
  • Superior staining capabilities
  • Can be reprobed multiple times
  • Compatible with a variety of detection chemistries, including radioactive, chromogenic, chemiluminescent, fluorescent, and chemifluorescent techniques

Immobilon® PVDF membranes offer excellent protein binding and high signal.

Immobilon® PVDF membranes have high protein adsorption, so you won’t lose proteins during transfer or reprobing. The open pore structure makes it easy to access bound proteins and remove unbound probes. In addition, Immobilon® PVDF membranes optimized for fluorescent blots dramatically increase signal for high sensitivity in quantitative, multiplexing applications.

 

Watch the following video to learn how the Immobilon®-E membrane provides the benefits of PVDF without an alcohol pre-wet step.