LifeNet Health®
Primary Human Hepatocytes

We provide high viability cryoplateable and cryopreserved  hepatocytes sourced from LifeNet Health® that predictably represent in vivo conditions. Applications for primary hepatocytes include in vitro evaluation of metabolism, drug-drug interactions, drug transporter activity, and toxicity of drug candidates. There are many other applications that encompass cryoplateable and cryopreserved hepatocytes.

LifeNet Health®, celebrating 35 years of saving lives, restoring health, and giving hope to thousands each year, is the world's most trusted provider of transplant solutions — from organ procurement to bio-implants and cellular therapies — and a leader in regenerative medicine, while always honoring the donors and healthcare professionals who enable healing.

LifeNet Health®’s primary human hepatocytes meet the specific needs of a wide range of scientific research applications, including drug development and risk assessment. Donated liver tissues are procured under state-of-the-art conditions using the highest standards for tissue recovery and preservation, which utilize enhanced tissue handling and transportation methods and minimize warm and cold ischemia times to optimize tissue processing outcomes and product integrity. These measures, combined with refined cell isolation techniques and advanced post-thaw characterization, represent a new industry standard for hepatocyte quality and performance.

Prior to release, each batch of cryopreserved hepatocytes is carefully characterized to determine the post-thaw results. The batch-specific functionality, donor medical history, and clinical data includes the following:

  • Cell viability and yield per vial
  • Morphological integrity and attachment efficiency
  • Optimal seeding density (based on a 24-well plate format)
  • CYP enzyme activity using prototype selective substrates for all the major enzymes pertinent to drug discovery and development
  • Additional functional testing can be performed upon request for specific cell culture applications
  • Each batch comes with a comprehensive Certificate of Analysis (CoA) with representative images, relevant donor demographics, BMI, pre-mortem liver function lab values, serological test results, and pertinent tobacco, alcohol, drug, and medication history
  • Liver biopsy histology images and pathology results may also be available upon request

The liver microanatomy: our guide for next gen model systems

Liver microanatomy


Click here (PDF) to get a complete list of currently available hepatocyte lots from LifeNet Health®.

Hepatocytes

Effects of Cell Adhesion on Cellular Response to Enzyme Inducers

Effects of Cell Adhesion on Cellular Response to Enzyme Inducers

Categories of Cryopreserved Human Hepatocyte Lots

The following are the major categories of cryopreserved hepatocytes that are currently available through LifeNet Health®. Additional options or categories, not listed below, may be considered upon individual request:

Adult Suspension/Metabolism Qualified:
Primary adult human hepatocytes are considered the preferred method for determining the metabolic stability and intrinsic clearance of new compounds in development. LifeNet Health®’s suspension batches of  cryopreserved hepatocytes are characterized for drug-metabolizing enzyme activity using prototype selective substrates for phase I and II metabolic pathways.

Adult Plateable/Induction Qualified:
Use of cultured primary human hepatocytes has become the accepted best practice for conducting in vitro testing of new drugs for their potential to be involved in unwanted drug-drug interactions due to induction of hepatic clearance pathways. LifeNet Health®’s induction qualified batches of cryopreserved hepatocytes are tested for response to prototype inducers of CYP1A2, CYP2B6, and CYP3A4. These prequalified lots are guaranteed to produce stable confluent monolayers for a minimum of 1 week and meet or surpass the induction specifications when used in conjunction with LifeNet Health®’s recommended culture conditions.

Adult High BMI/NAFLD/NASH:
Non-alcoholic fatty liver disease (NAFLD) is the most common chronic liver disease in Western countries with a wide disease spectrum. For LifeNet Health®’s Adult High BMI/NAFLD/NASH hepatocytes (suspension and plateable), a
histopathological evaluation of formalin-fixed, paraffin-embedded tissue sections after H&E and trichrome staining is performed by a certified pathologist. Representative histological images and a pathology report can be provided with each CoA upon request.

Donor LHuf15102: BMI = 37; Z1-2 microfat = 50%

Donor LHuf15102: BMI = 37; Z1-2 microfat = 50%


Neonatal/Pediatric/Juvenile:

It is becoming more and more important in risk assessment to understand the developmental changes in the expression of phase I and II enzymes and other clearance mechanisms at different life stages that determine the pharmaco- and toxicokinetics of chemicals.

Normal Human Hepatocytes

‘Normal’ Human Hepatocytes
 

Steatotic Human Hepatocytes

‘Steatotic’ Human Hepatocytes

Features and Benefits

  • Drug metabolism studies including stability, clearance, species comparison,
  • Metabolite profiling and ID
  • Metabolism of low clearance compounds
  • CYP induction (mRNA and enzyme activity)
  • Plated and suspension drug transporter assays (uptake and efflux)
  • Hepatotoxicity assays
  • High content imaging and screening
  • 3D culture technologies

Importance of Restoring Hepatocyte Physiology for In Vitro Cell Culture Studies

Importance of Restoring Hepatocyte Physiology for In Vitro Cell Culture Studies

The most accurate picture for hepatic effects can be obtained with cultures of primary cells in which the native architecture and the natural orientation for linked enzymes, transporters and receptors are restored.

Click here (PDF) to get a complete list of currently available hepatocyte lots from LifeNet Health®.

Materials

     
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