MIQE – Minimum Information for Publication of Quantitative Real-Time PCR Experiments

The potential applications for quantitative real-time PCR (qPCR) have increased exponentially since the first description (Higuchi, 1993). However, researchers have been frustrated by complications such as contamination, insufficient amplification, low sensitivity, and uncertainty about what constitutes a suitable statistical analysis. Until recently, there has been a lack of consensus about how to deal with these obstacles.

An international research team published The MIQE (pronounced Mykee) Guidelines in 2009 to address the challenges of performing dependable qPCR measurements. By following The MIQE Guidelines, you will:

  • Promote experimental transparency
  • Ensure consistency between laboratories
  • Maintain the integrity of the scientific literature

Publish or bring your product to market faster and with confidence by adhering to The MIQE Guidelines.

Materials