Muse® Assays for Immunology

Immunology deals with the functioning of the immune system in states of both health and disease and its physical, chemical and physiological characteristics and components. Immune cells interact with one another by a variety of signal molecules so that a coordinated response may be mounted against a pathogen or antigen. Flow cytometric analysis has always been a core technique for studying immune cells; now, the Muse® system brings the analyses you perform the most right to your bench top.

Existing Muse® Immunology Assays:

  • Muse® Human CD4 T Cell Kit
  • Muse® Human CD8 T Cell Kit
  • Muse® Human B Cell Kit
  • Muse® Human CD25 Lymphocyte Kit (for lymphocyte activation studies)
  • Muse® Human CD69 Lymphocyte Kit (for lymphocyte activation studies)

We are continually releasing new Muse® assay modules and kits. Please visit www.emdmilllipore.com/muse for the most up-to- date listing of Muse® Assays. New assay software modules can be downloaded free of charge from the website.

Featured Kits

Muse® Human CD4 T Cell Kit (Cat. No. MIM100101)

Quickly determine the CD4 T-cell count, total lymphocyte count, and CD4 T-cell percent of lymphocytes in human whole blood and peripheral blood mononuclear cell (PBMC) samples. Monocytes also express CD4 but at a lower density, and have no co-expression of the other antibodies present in the anti-lymphocyte cocktail; hence, monocytes can be distinguished from CD4 T cells using this kit. The Muse® CD4 T Cell Assay uses an anti-lymphocyte antibody cocktail that identifies the lymphocyte population, and CD4 antibody that binds to the CD4 cells. Minimal sample preparation is required in this no-wash assay to obtain accurate and precise results. For research use only; not for use in diagnostic procedures.

Data generated include:

  • CD4 T-cell concentration in cells/µL
  • CD4 T-cell percent of lymphocytes
  • Total lymphocyte concentration in cells/µL

Data output for Muse® Human CD4 T Cell Kit. Whole blood was stained with the Muse® Human CD4 T Cell Kit and analyzed on the Muse® Cell Analyzer. The statistics show the percentages and the concentrations (cells/µL) for the results in each quadrant out of the total events (top). The lymphocyte results show the total lymphocyte concentration, the CD4 T-lymphocyte concentration and the CD4 cell percentage of lymphocytes. The first plot in the bottom panel shows lymphocyte count vs. cell size index and a lymphocyte gate, and the second plot shows lymphocyte count vs. CD4 staining.Muse® Human B Cell Kit (Cat. No. MIM100103)

Determine the B-cell count, total lymphocyte count, and B-cell percent of lymphocytes in human whole blood and peripheral blood mononuclear cell (PBMC) samples. The Muse® Human B Cell Kit detects and identifies lymphocytes and CD19 B lymphocytes in either whole blood or PBMCs. CD19 is considered to be a characteristic B cell marker and therefore commonly used in the routine immunophenotyp-ing of B cells. Minimal sample preparation is required in this no-wash assay to obtain accurate and precise results.

Data generated include:

  • B-cell concentration in cells/µL
  • B-cell percent of lymphocytes
  • Total lymphocyte concentration in cells/µL

Data output for Muse® Human B Cell Kit. Whole blood was stained with the Muse® Human B Cell Kit and analyzed on the Muse® Cell Analyzer. The statis-tics show the percentages and the concentrations (cells/µL) for the results in each quadrant out of the total events. The lymphocyte results show the total lympho-cyte concentration, the B-lymphocyte concentration, and the B-cell percentage of lymphocytes.

Muse® Human CD69 Lymphocyte Kit for Lymphocyte Activation Studies (Cat. No. MIM100104)

Determine the CD69(+) lymphocyte count, total lympho-cyte count, and CD69 percent of total lymphocytes in human whole blood and peripheral blood mononuclear cell (PBMC) samples. CD69 is an early-activation marker expressed on the surface of activated lymphocytes includ-ing T and B lymphocytes and NK cells. The Muse® Human Lymphocyte CD69 Assay uses an anti-lymphocyte cocktail that identifies the total lymphocyte population and a CD69 antibody that binds to the CD69 early activation marker expressed on lymphocytes. The kit also includes an isotype control antibody. Minimal sample preparation is required in this no-wash assay to obtain accurate and precise results.

Data generated include:

  • CD69(+) lymphocyte concentration in cells/µL
  • CD69 percent of total lymphocytes
  • Total lymphocyte concentration in cells/µL

Data output for Muse® Human CD69 Kit. Data output for Muse® Human CD69 Kit. Whole blood was stained with the Muse® Human Lymphocyte CD69 Kit and analyzed on the Muse® Cell Analyzer. The statistics show the percentages and the concentrations (cells/µL) for the re-sults in each quadrant out of the total events (screen not shown). The lymphocyte results show the total lymphocyte concentration, the CD69(+) lymphocyte concen-tration, and the CD69 percentage of total lymphocytes. The first plot (left panel) shows cell size index vs. lymphocytes and a lymphocyte gate, and the second plot shows CD69 staining vs. lymphocytes.

     
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