N,N-Dimethylformamide Molecular Biology Reagent

Product No. D4551

Synonym: DMF

Product Description

DMF is a polar organic solvent that is widely used in the synthesis of organic compounds. The high solvent power and stability of DMF at elevated temperatures contribute to its utility. DMF is a solvent for vinyl resins, acetylene, butadiene, and polyacrylic fibers. It is also a catalyst in carboxylation reactions.1,2

DMF is also utilized in chromatographic applications such as HPLC, capillary electrochromatography, and capillary electrophoresis.3,4,5 A capillary electrophoresis study has been published on the mobility of large organic cations in such non-aqueous solvents as DMF.6 DMF has been used for the separation of aliphatic amines in microfabricated capillary electrophoresis devices.7

CNBr-activated diol-silica supports for high performance affinity chromatography have been prepared with DMF.8 DMF has also been used for peptide and protein crystallization.9,10

Precautions and Disclaimer

This product is for R&D use only, not for drug, household, or other uses. Please consult the Safety Data Sheet for information regarding hazards and safe handling practices.

Preparation Instructions

This product is freely miscible with water and with most common organic solvents, including alcohols, esters, ethers, ketones, and chlorinated and aromatic hydrocarbons. The pH of a 0.5 M DMF aqueous solution is 6.7.1

Product Profile

Molecular Formula: C3H7NO
Molecular Weight: 73.10
CAS Number: 68-12-2
Melting Point: -61 °C1
Boiling Point: 153 °C1
Density: 0.9445 g/ml (25 °C)1
Synonyms: DMF, DMFA1


This product is designated as molecular biology grade. It has been tested to be suitable as a solvent for chromogenic substrates (X-Gal) in molecular biology applications.




  1. The Merck Index, 12th ed., Entry# 3292.
  2. Hawley's Condensed Chemical Dictionary, Lewis, R. J., Sr., ed., John Wiley and Sons (New York: 2001), p. 396.
  3. Du, C. M., et al., Characterizing the selectivity of stationary phases and organic modifiers in reversed-phase high-performance liquid chromatographic systems by a general salvation equation using gradient elution. J. Chromatogr. Sci., 38(11), 503-511 (2000).
  4. Roed, L., et al., Nonaqueous electrochromatography on C30 columns: separation of retinyl esters. Electrophoresis, 20(12), 2373-2378 (1999).
  5. Muzikar, J., et al., Extension of the application range of UV-absorbing organic solvents in capillary electrophoresis by the use of a contactless conductivity detector. J. Chromatogr. A., 924(1-2), 147-154 (2001).
  6. Muzikar, J., et al., Electrophoretic mobilities of large organic ions in nonaqueous solvents: determination by capillary electrophoresis in propylene carbonate, N,N-dimethylformamide, N,N,-dimethylacetamide, acetonitrile and methanol. Electrophoresis, 23(3), 375-382 (2002).
  7. Wang, J., and Pumera, M., Nonaqueous electrophoresis microchip separations: conductivity detection in UV-absorbing solvents. Anal. Chem., 75(2), 341-345 (2003).


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