Oligonucleotide Quality Control by Polyacrylamide Gel Electrophoresis

Our different manufacturing sites may use PAGE (polyacrylamide gel electrophoresis) to analyze duplexes depending on the nature of the sequences. PAGE separates duplexes on the basis of molecular weight as opposed to charge (Figure 1). The migration of the siRNA duplexes is compared to standards, ensuring siRNA simplexes are properly annealed and ready for transfection.

Separation via PAGE.

Figure 1. Separation via PAGE.

Conclusion

Depending on manufacturing site, we may use PAGE to assess siRNA duplexes. If additional help is needed, please consult our technical services group at oligotechserv@sial.com.

Materials