Onyx Quencher™ FAQ (Frequently Asked Questions)

What is the molecular weight of OQ™?
The molecular weights of OQ™A, OQ™B, OQ™C, and OQ™D are 518.6, 589.6, 582.6, and 650.7, respectively.

Is the molecular structure of OQ™ available?
No, structures of the OQ™ derivatives are proprietary and therefore are not available for distribution.

Is OQ™ available as an internal modification?
No, OQ is only available on the 3’ ends of Custom Primers, Dual-Labeled Probes, and Molecular Beacons.

What is the difference between OQ™ and TAMRA™?
OQ™ is a dark quencher that releases heat instead of light upon excitation, whereas TAMRA is a fluorescent reporter dye. While TAMRA generally works well with 6-FAM™, its fluorescence can lead to poor signal-to-noise ratios depending on the nature of the assay.

How should I store OQ™-labeled oligos and probes?
OQ™-labeled oligos and probes ship dry (and in amber tubes (plates are not amber) for probes to prevent photodegradation of the reporter dye) and should be stored at -20 °C. When ready for use, prepare a 100 µM stock solution by resuspending in a weak buffer such as TE (10 mM Tris, pH 7.5 - 8.0, 1 mM EDTA or for applications in which TE is not suitable, sterile nuclease-free water is a good choice. For additional details, please see our Oligonucleotide Handling & Stability article.

Materials