With over 1600 vectors to choose from, your options may seem endless. For help choosing the right vector for your research needs, consult the tables below.
Vector options for single functional tags for use in yeast cells
Yeast Plasmids Encoding Single Functional Tags
N Terminal Tag Plasmids are in Blue
C Terminal Tag Plasmids are in Red
Vector options for dual functional tags for use in yeast cells
Yeast Plasmids Encoding Dual Functional Tags
N Terminal Tag Plasmids are in Blue
C Terminal Tag Plasmids are in Red
Vector options for fluorescent reporters
Fluorescence Reporter Gene Plasmids
| |
GFP
(DaGFP) |
YFP
(KrYFP) |
CFP
(FrCFP) |
| Single Gene Vectors |
Choose a promoter to drive the reporter gene |
CMV Promoter |
OGS242 |
OGS510 |
OGS509 |
| Minimal CMV Promoter |
OGS573 |
OGS574 |
OGS575 |
| Systems for you to insert your own promoter |
With polyA upstream of MCS - to minimise background |
Prom-MCS-pAa |
OGS241 |
OGS614 |
OGS615 |
| MinProm-pAb |
OGS251 |
– |
– |
| CMVe-pAc |
OGS257 |
– |
– |
| Without upstream pA - better for cloning into retrovirus or lentivirus |
Prom MCS (no pA)a |
OGS347 |
– |
– |
| MinProm (no pA)b |
OGS362 |
– |
– |
| CMVe (no pA)c |
OGS368 |
– |
– |
| Double Gene Vectors (place your gene of interest into the MCS, under CMV promoter control) |
Chose a promoter to drive the reporter gene |
RSV |
OGS243 |
OGS516 |
OGS515 |
| Ubiquitin |
|
OGS513 |
OGS512 |
| PGK |
OGS390 |
OGS611 |
OGS610 |
| IRES (Internal Ribosome Entry Site) |
FMDV |
OGS289 |
OGS521 |
OGS522 |
| EMCV |
OGS315 |
OGS518 |
OGS519 |
| Reporter Fusion Protein Contructs |
See separate table |
NOTES:
(a)PromMCS plasmids have no promoter driving the reporter gene, but they have several unique restriction sites that can be used to insert the promoter of your choice.
(b)MinProm plasmids contain the minimal HSV TK promoter driving the reporter gene, with an upstream MCS that can be used to introduce specificity-defining sequences (such as transcription factor binding sites) of your choice
(c)CMVe plasmids contain the CMV enhancer upstream of the MCS, for your to insert your promoter of choice. The CMV enhancer should augment its activity.
Vector options for fusion reporters
Mammalian Reporter Fusion Protein Plasmids
N Terminal Tag Plasmids are in Blue
C Terminal Tag Plasmids are in Red
Vector options for the Luciferase reporter
Luciferase Reporter Gene Plasmids
NOTES:
(a)PromMCS plasmids have no promoter driving the reporter gene, but they have several unique restriction sites that can be used to insert the promoter of your choice.
(b)MinProm plasmids contain the minimal HSV TK promoter driving the reporter gene, with an upstream MCS that can be used to introduce specificity-defining sequences (such as transcription factor binding sites) of your choice
(c)CMVe plasmids contain the CMV enhancer upstream of the MCS, for your to insert your promoter of choice. The CMV enhancer should augment its activity.
Vector options for Colorimetric reporters
Colorimetric Reporter Gene Plasmids
NOTES:
(a)PromMCS plasmids have no promoter driving the reporter gene, but they have several unique restriction sites that can be used to insert the promoter of your choice.
(b)MinProm plasmids contain the minimal HSV TK promoter driving the reporter gene, with an upstream MCS that can be used to introduce specificity-defining sequences (such as transcription factor binding sites) of your choice
(c)CMVe plasmids contain the CMV enhancer upstream of the MCS, for your to insert your promoter of choice. The CMV enhancer should augment its activity.
Looking for more vector options to move your experiments forward faster? Consider a custom cloning vector designed and built by Oxford Genetics™. Find out more at Oxford Genetics - Sigma's partner for cloning and expression vectors for molecular biology and synthetic biology applications.
