Roche PCR Reagents and PCR Selection Guide

Roche PCR Reagents Selection Guide for Routine and Hot Start PCR

For additional information on Hot Start PCR technology, protocols, and to download our free Hot Start PCR eBook, please visit our Hot Start PCR resource guide.

 

PCR Application Routine PCR Fast PCR Hot Start Multiplex/Sequencing
Your Enzyme of Choice: Taq DNA Polymerase AptaTaq™ Fast
DNA Polymerase
FastStart™ Taq
DNA Polymerase
FastStart™ High
Fidelity PCR System
Experience Consistency Speed Specificity Accuracy
Amplicon Size up to 3 kb up to 500 bp up to 3 kb up to 5 kb
Specificity
Sensitivity
Robustness
Accuracy vs. Taq 1x 1x 1x 4x
Carryover Prevention yes yes yes yes
Units/50 μl 1.25 2/20 μl 2 2.5
Molecular Cloning TA cloning TA cloning TA cloning TA cloning
Enzyme Taq DNA
  Polymerase,
  5 U/μl

Taq DNA
  Polymerase,
  1 U/μl
AptaTaq™
  Fast DNA
  Polymerase,
  1 U/μl
FastStart™
  Taq DNA
  Polymerase,
  5 U/μl
FastStart™
  High Fidelity
  PCR System
Enzyme, GMP Grade • Taq DNA
  Polymerase,
  GMP Grade,
  5 U/μl
     
dNTPack Taq DNA
  Polymerase,
  dNTPack, 5 U/μl

Taq DNA
  Polymerase,
  dNTPack, 1 U/μl
  FastStart™
  Taq DNA
  Polymerase,
  dNTPack,
  5 U/μl
FastStart™
  High Fidelity
  PCR System, dNTPack
Master Mix PCR Master AptaTaq™
  Fast PCR
  Master
FastStart™
  PCR Master
 

 

Roche PCR Reagents Selection Guide for High Fidelity, Difficult Templates, and Long PCR Amplification

 

PCR Application Wide Variety/Easy Access High Fidelity Difficult Templates Long Range
Your Enzyme of Choice: Expand™ High Fidelity
PCR System
Pwo SuperYield DNA Polymerase GC-RICH PCR System Expand™ Long Range
Experience Robustness Fidelity Reliability Versatility
Amplicon Size up to 5 kb up to 3 kb up to 5 kb 5 to 25 kb**
Specificity
Sensitivity
Robustness
Accuracy vs. Taq 3x 18x 3x 3x
Carryover Prevention no* no no no
Units/50 μl 2.6 2.5 2 3.5
Molecular Cloning TA cloning Blunt end cloning TA cloning preferred over blunt end cloning TA cloning
Enzyme Expand™ High
  Fidelity
  PCR System

Expand™ High
  FidelityPLUS
  PCR System
Pwo SuperYield
  DNA
  Polymerase
GC-RICH PCR System  
dNTPack Expand™ High
  Fidelity
  PCR System,
  dNTPack

Expand™ High
  FidelityPLUS PCR
  System, dNTPack
Pwo SuperYield
  DNA
  Polymerase,
  dNTPack
GC-RICH PCR
  System,
  dNTPack
Expand™
  Long Range,
  dNTPack
Master Mix High Fidelity PCR
  Master
Pwo Master    

* Requiring carryover prevention and 6x higher fidelity? Choose Expand™ High FidelityPLUS PCR System.
** For amplicon sizes of 20-35 kb choose Expand™ 20 kbPLUS PCR System.

Cutting-edge science shouldn’t have to compromise for the sake of economy. Since the introduction of PCR technology, Roche has provided the gold standard in PCR reagents. Our advanced isolation, purification, and manufacturing techniques ensure that you receive not only the clearest, most reliable results, but also convenience and affordability to fit any laboratory budget.

  • Reliability – Achieve the results you deserve, from lot to lot, tube to tube, and experiment to experiment.
  • Performance – Combine premium enzymes plus PCR-Grade Nucleotides in convenient dNTPacks for enhanced sensitivity and yield.
  • Consistency – Maximize experimental performance using master mixes that only require the addition of primers and template.

PCR-Grade Nucleotides ensure optimal performance.

PCR success is highly dependent on your selection of both enzymes and nucleotides. Roche PCR enzymes are available in convenient dNTPacks, which include premixed solutions of additive-free sodium salt nucleotides. These function tested PCR-Grade Nucleotides are manufactured by enzymatic synthesis and purified by a unique process and are an ideal contributor to the quality of your PCR results.

  • Purity – Choose nucleotides free of modified bases, tetraphosphates, or pyrophosphate contaminants according to current quality procedures typical to chemically synthesized nucleotide preparations to improve your PCR’s sensitivity.
  • Stability – Lengthen shelf life and improve reaction stability using dNTPs supplied at an optimal pH to maximize yield.

Taq DNA Polymerase and Standard PCR Protocol

Affordable PCR characterized by robust amplification with minimal template requirements.

Roche Taq DNA Polymerase is produced under GMP conditions. This highly purified enzyme passes several stringent tests for functionality and purity, ensuring reliable, consistent results with every lot. For additional information, please view our standard PCR protocol.

  • Obtain reliable, reproducible results with high lot-to-lot consistency
  • Eliminate testing of each new lot – we do it for you
  • Combine dUTP incorporation with Uracil-DNA Glycosylase to prevent PCR cross-contamination
 

Figure 1. Lot-to-lot consistency ensures reproducible results. Five different lots of Taq DNA Polymerase were tested for the ability to amplify a 0.5 kb fragment of lambda DNA. Reliable, consistent results are obtained with every lot of Roche Taq DNA Polymerase that was tested.

The Taq DNA Polymerase, GMP Grade belongs to the family of high-performance, validated amplification enzymes and is manufactured using evaluated production, quality control, and filling procedures.

 
Amplicon Size Specificity Sensitivity Robustness Accuracy
vs. Taq
Carryover Prevention Units
/50 µL
Molecular Cloning
Up to 3 kb 1x yes 1.25 TA cloning

 

Product SKU Pack Size
Taq DNA Polymerase | Enzyme + Buffer
5 U/μL 11 146 173 001 500 U for up to 1,000 reactions of 20 μL final volume, each containing 0.5 U Taq DNA Polymerase
11 418 432 001 1,000 U (4 × 250 U) for up to 2,000 reactions of 20 μL final volume, each containing 0.5 Taq DNA Polymerase
11 596 594 001 2,500 U (10 × 250 U) for up to 5,000 reactions of 20 μL final volume, each containing 0.5 U Taq DNA Polymerase
11 435 094 001 5,000 U (20 × 250 U) for up to 10,000 reactions of 20 μL final volume, each containing 0.5 U Taq DNA Polymerase
1 U/μL 11 647 687 001 1,000 U (4 × 250 U) for up to 2,000 reactions of 20 μL final volume, each containing 0.5 U Taq DNA Polymerase
Taq DNA Polymerase, dNTPack | Enzyme + Buffer + dNTP Set
5 U/μL 04 728 866 001 100 U for up to 200 reactions of 20 μL final volume, each containing 0.5 U Taq DNA Polymerase
04 728 874 001 500 U (2 × 250 U) for up to 1,000 reactions of 20 μL final volume, each containing 0.5 U Taq DNA Polymerase
04 728 882 001 1,000 U (4 × 250 U) for up to 2,000 reactions of 20 μL final volume, each containing 0.5 U Taq DNA Polymerase

04 728 904 001 2,500 U (10 × 250 U) for up to 5,000 reactions of 20 μL final volume, each containing 0.5 U Taq DNA Polymerase
04 728 858 001 5,000 U (20 × 250 U) for up to 10,000 reactions of 20 μL final volume, each containing 0.5 U Taq DNA Polymerase
1 U/μL 04 738 225 001 250 U for up to 500 reactions of 20 μL final volume, each containing 0.5 U Taq DNA Polymerase
04 738 241 001 1,000 U (4 × 250 U) for up to 2,000 reactions of 20 μL final volume, each containing 0.5 U Taq DNA Polymerase
PCR Master | Ready-to-use Formulation; Enzyme, Buffer, dNTPs
  11 636 103 001 1 kit for up to 200 PCR reactions of 50 μL final reaction volume, each containing 2.5 U Taq DNA Polymerase

AptaTaq™ Fast DNA Polymerase and Master Mix

Accelerate your reaction, improve performance and achieve results comparable to standard reactions in a fraction of the time.

AptaTaq™ Fast PCR Master and AptaTaq™ Fast DNA Polymerase are ready-to-use products for fast PCR block cycling. Obtain high specificity and unmatched speed with AptaTaq™ Polymerase’s immediate enzyme activation right from the cycling start. The product is function tested using block cycler and qPCR.

  • Save precious time. Complete end-point PCR reactions faster with a highly processive enzyme requiring only 4 minutes of extension time and without assay optimization.
  • Choose an easy PCR setup. Set up PCR reaction at room temperature, not on ice.
  • Redefine Fast PCR. Successfully amplify a large variety of templates with different GC-content using only one protocol.
Figure 2. Perform PCR faster than with other suppliers’ “fast” PCR enzymes. The AptaTaq enzyme amplifies a 195 bp fragment from the human erythropoietin gene 18 – 36% faster, with only 4 minutes of total extension time through 35 cycles.
 
Figure 3. Amplification of a broad range of fragments, even those with up to 66% GC content. Gel photo shows amplification on one PCR Multiwell Plate within one block cycler instrument.
 

 

Amplicon Size Specificity Sensitivity Robustness Accuracy
vs. Taq
Carryover Prevention Units
/50 µL
Molecular Cloning
Up to 500 bp 1x yes 2 µL TA cloning

 

Product SKU Pack Size
AptaTaq™ Fast DNA Polymerase | Enzyme + Buffer
1 U/μL 06 879 110 001 100 units
06 879 128 001 1000 units
AptaTaq™ Fast PCR Master | Ready-to-use Formulation; Enzyme, Buffer, dNTPs
  06 879 080 001 1 × 400 μL for up to 100 reactions of 20 μL final reaction volume
  06 879 101 001 10 × 400 μL for up to 1000 reactions of 20 μL final reaction volume

 

FastStart™ Taq DNA Polymerase and Master Mix

Choose the polymerase researchers trust, as with qPCR and the LightCycler® Systems.

FastStart™ Taq DNA Polymerase is a thermostable, chemically modified form of recombinant Taq DNA polymerase. Due to its modification, FastStart™ Taq DNA polymerase is only activated at high temperatures, making it the ideal enzyme when your assay’s amplification is delayed.

  • Obtain higher specificity, sensitivity, and yield. Hot start PCR improves PCR performance, making PCR setup even easier.
  • Use a convenient robotic setup. The complete reaction mix is stable for 24 hours at room temperature.
  • Prevent PCR carryover. Combine dUTP incorporation with Uracil-DNA Glycosylase to prevent PCR cross-contamination.
Figure 4.  Amplification of the human erythropoietin gene from 3-300 ng cDNA after immediate (E0) and 24 hours after reaction setup at room temperature (E24).
 
 
Amplicon Size Specificity Sensitivity Robustness Accuracy
vs. Taq
Carryover Prevention Units
/50 µL
Molecular Cloning
Up to 3 kb 1x yes 2 µL TA cloning

 

Product SKU Pack Size
FastStart™ Taq DNA Polymerase | Enzyme + Buffer
5 U/μL 12 032 929 001 500 U (2 × 250 U) for up to 250 reactions of 50 μL final volume, each containing 2 U FastStart Taq DNA Polymerase
12 032 937 001 1,000 U (4 × 250 U) for up to 500 reactions of 50 μL final volume, each containing 2 U FastStart Taq DNA Polymerase
12 032 945 001 2,500 U (10 × 250 U) for up to 1,250 reactions of 50 μL final volume, each containing 2 U FastStart Taq DNA Polymerase
12 032 953 001 5,000 U (20 × 250 U) for up to 2,500 reactions of 50 μL final volume, each containing 2 U FastStart Taq DNA Polymerase
FastStart™ Taq DNA Polymerase, dNTPack | Enzyme + Buffer + dNTP Set
5 U/μL 04 738 314 001 100 U for up to 50 reactions of 50 μL final volume, each containing 2 U FastStart Taq DNA Polymerase
04 738 357 001 500 U (2 × 250 U) for up to 250 reactions of 50 μL final volume, each containing 2 U FastStart Taq DNA Polymerase
04 738 381 001 1,000 U (4 × 250 U) for up to 500 reactions of 50 μL final volume, each containing 2 U FastStart Taq DNA Polymerase
04 738 403 001 2,500 U (10 × 250 U) for up to 1,250 reactions of 50 μL final volume, each containing 2 U FastStart Taq DNA Polymerase
04 738 420 001 5,000 U (20 × 250 U) for up to 2,500 reactions of 50 μL final volume, each containing 2 U FastStart Taq DNA Polymerase
FastStart™ PCR Master | Ready-to-use Formulation; Enzyme, Buffer, dNTPs
  04 710 436 001 2 × 1.25 mL for up to 250 reactions of 20 μL final reaction volume
04 710 444 001 8 × 1.25 mL for up to 1,000 reactions of 20 μL final reaction volume
04 710 452 001 10 × 5 mL for up to 5,000 reactions of 20 μL final reaction volume

 

FastStart™ High Fidelity PCR System

Multiplexing and sequencing stringent hot start and improved fidelity is key in complex endpoint PCR applications, such as multiplexing or sequencing. Choose FastStart™ High Fidelity PCR System for complex hot start PCR up to 5 kb with higher fidelity, as required for multiplexing and sequencing. It is up to six times more accurate compared to both standard Taq DNA polymerase and FastStart™ Taq DNA Polymerase.

  • Achieve excellent multiplex performance with fast, high-quality results
  • Experience six-fold higher fidelity compared to Taq and FastStart™ Taq DNA Polymerase
  • Use the supplied PCR additive (DMSO) when amplifying difficult templates

Sensitivity test in 18-plex (74 bp – 470 bp) PCR. Set of 18 multiplexed primers was applied to various concentrations of human genomic DNA.

Figure 5. Sensitivity test in 18-plex (74 bp – 470 bp) PCR. Set of 18 multiplexed primers was applied to various concentrations of human genomic DNA.

 

Amplicon Size Specificity Sensitivity Robustness Accuracy
vs. Taq
Carryover Prevention Units
/50 µL
Molecular Cloning
Up to 5 kb 6x yes 2.5 TA cloning

 

Product SKU Pack Size
FastStart™ High Fidelity PCR System | Enzyme + Buffer
  03 553 400 001 500 U (2 × 250 U) for up to 200 reactions of 50 μL final volume, each containing 2.5 U FastStart Taq DNA Polymerase
03 553 361 001 2,500 U (10 × 250 U) for up to 1,000 reactions of 50 μL final volume, each containing 2.5 U FastStart Taq DNA Polymerase
FastStart™ High Fidelity PCR System, dNTPack | Ready-to-use Formulation; Enzyme, Buffer, dNTPs
  04 738 284 001 125 U for up to 50 reactions of 50 μL final volume, each containing 2.5 U FastStart Taq DNA Polymerase
04 738 292 001 500 U (2 × 250 U) for up to 200 reactions of 50 μL final volume, each containing 2.5 U FastStart Taq DNA Polymerase

Expand™ High Fidelity PCR System and Expand™ High FidelityPLUS PCR System

Expand™ High Fidelity PCR reagents are designed for robust amplification across a broad range of assays and amplicon types, consisting of an enzyme blend of Taq DNA Polymerase and a polymerase with proofreading activity for robust PCR. Choose the Expand™ High FidelityPLUS PCR System for robust PCR up to 5 kb with PCR carryover prevention.

  • Use one enzyme for all applications. The Expand™ High Fidelity PCR System robustly amplifies a wide variety of templates ensuring high yield, fidelity, and flexibility.
  • Detect amplification products that were previously undetectable and avoid false negatives.
  • Achieve successful PCR results from small quantities of template DNA.

The Expand™ High FidelityPLUS PCR System is ideal for robust high-fidelity applications, such as cloning and labeling of DNA fragments with radioactively or nonradioactively modified nucleotides. In addition, combine dUTP incorporation with uracil-DNA glycosylase to prevent PCR cross-contamination.

Comparison of Expand High FidelityPlus PCR System
with four commercially available polymerase mixes.

Figure 6. Comparison of Expand™ High FidelityPlus PCR System with four commercially available polymerase mixes. Various amounts (ng) of human genomic DNA were used to amplify a 4.8 kb fragment from the tissue plasminogen activator (tPA) gene, in accordance with each manufacturer's recommended conditions. Expand High FidelityPLUS PCR System produces the best specificity, sensitivity, and yield, even from as little as 1 ng human genomic DNA.

Supplier A: Mixture of Taq DNA polymerase (deleted at N-terminus), a proofreading polymerase, and a hot start antibody.
Supplier B and D: Mixture of Taq DNA polymerase and a proofreading polymerase.
Supplier C: Mixture of Taq DNA polymerase, a proofreading polymerase, and an enhancing factor.

 

Amplicon Size Specificity Sensitivity Robustness Accuracy
vs. Taq
Carryover Prevention Units
/50 µL
Molecular Cloning
Up to 5 kb 3x no* 2.6 TA cloning

 

Product SKU Pack Size
Expand™ High Fidelity PCR System | Enzyme + Buffer
  11 732 641 001 100 U for up to 40 reactions of 50 μL final volume, each containing 2.6 U enzyme blend
11 732 650 001 500 U (2 × 250 U) for up to 200 reactions of 50 μL final volume, each containing 2.6 U enzyme blend
11 759 078 001 2,500 U (10 × 250 U) for up to 1,000 reactions of 50 μL final volume, each containing 2.6 U enzyme blend
Expand™ High Fidelity PCR, dNTPack | Enzyme + Buffer + dNTP Set
  04 738 250 001 100 U for up to 40 reactions of 50 μL final volume, each containing 2.6 U enzyme blend
04 738 268 001 500 U (2 × 250 U) for up to 200 reactions of 50 μL final volume, each containing 2.6 U enzyme blend
04 738 276 001 2,500 U (10 × 250 U) for up to 1,000 reactions of 50 μL final volume, each containing 2.6 U enzyme blend
High Fidelity PCR Master | Ready-to-use Formulation; Enzyme, Buffer, dNTPs
  12 140 314 001 1 kit for up to 500 reactions of 20 μL final reaction volume

 

Product SKU Pack Size
Expand™ High FidelityPLUS PCR System | Enzyme + Buffer
  03 300 226 001 500 U (2 × 250 U) for up to 500 reactions of 20 μL final volume, each containing 1 U enzyme blend
03 300 234 001 2,500 U (10 × 250 U) for up to 2,500 reactions of 20 μL final volume, each containing 1 U enzyme blend
Expand™ High FidelityPLUS PCR System, dNTPack | Enzyme + Buffer + dNTP Set
  04 743 725 001 125 U for up to 125 reactions of 20 μL final volume, each containing 1 U enzyme blend
04 743 733 001 500 U (2 × 250 U) for up to 500 reactions of 20 μL final volume, each containing 1 U enzyme blend

Pwo SuperYield DNA Polymerase

High fidelity PCR is required in applications where sequence accuracy is crucial without sacrificing yield. Use Pwo SuperYield DNA Polymerase to obtain high yields of PCR product with consistent high fidelity. Choose maximum fidelity and avoid sequence errors, base exchanges, and frame shifts when combined with a proofreading reverse transcriptase, such as in the Transcriptor High Fidelity cDNA Synthesis Kit. The combination is ideal for applications such as cloning, site-directed mutagenesis, and gene expression analysis.

  • Achieve excellent fidelity and high yields. 18-fold higher fidelity compared to Taq DNA Polymerase, without optimization.
  • Obtain high performance with difficult templates. The GC-RICH Solution enables amplification of GC-rich DNA.
  • Reduce working steps in cloning. Perform enzyme digests directly in Pwo SuperYield PCR mix.

Improved accuracy in RT-PCR. Combination of the proofreading reverse transcriptase, Transcriptor High Fidelity Reverse Transcriptase (Roche), and a proofreading polymerase, Pwo SuperYield DNA Polymerase (Roche) for amplification.


Figure 7. Improved accuracy in RT-PCR. Combination of the proofreading reverse transcriptase, Transcriptor High Fidelity Reverse Transcriptase (Roche), and a proofreading polymerase, Pwo SuperYield DNA Polymerase (Roche) for amplification. Data compared to a commonly used M-MuLV reverse transcriptase and Taq DNA polymerase. Error rate was determined after reverse transcription and 25 PCR cycles using the 454 Sequencing System. The error rates for the Roche enzymes was the mean value of four independent experiments in which at least 3.1 × 106 bases were sequenced. For M-MuLV reverse transcriptase and Taq DNA Polymerase, 4.5 × 106 bases were sequenced. The accuracy is represented as error rate -1.

Amplicon Size Specificity Sensitivity Robustness Accuracy
vs. Taq
Carryover Prevention Units
/50 µL
Molecular Cloning
Up to 3 kb 18x no 2.5 blunt end cloning

 

Product SKU Pack Size
Pwo Super Yield DNA Polymerase | Enzyme + Buffer
  04 340 850 001 500 U (2 × 250 U) for up to 200 reactions of 50 μL final volume, each containing 2.5 U Pwo SuperYield DNA Polymerase
Pwo Super Yield DNA Polymerase, dNTPack | Enzyme + Buffer + dNTP Set
  04 743 750 001 100 U for up to 40 reactions of 50 μL final volume, each containing 2.5 U Pwo SuperYield DNA Polymerase
Pwo Master | Ready-to-use Formulation; Enzyme, Buffer, dNTPs
  03 789 403 001 2.5 ml (10 × 250 μL) for up to 100 reactions of 50 μL final reaction volume, each containing 2.5 U Pwo SuperYield DNA Polymerase

GC-RICH PCR System for Difficult Template PCR

Choose the GC-RICH PCR System, a blend of a proofreading polymerase and Taq DNA Polymerase to power through templates that are difficult or impossible to amplify with other polymerases or other blends of polymerases and additives. The enhanced processivity of the blend and the unique GC-RICH Resolution Solution are combined to deliver superior performance.

  • Amplify difficult templates, including GC-rich targets and repetitive sequences
  • Use the supplied PCR Grade Water and optimized reagents, including the GC-RICH Resolution Solution
  • Amplify DNA fragments up to 5 kb

Successfully amplify GC-rich templates with the GC-RICH PCR System.

Figure 8. Successfully amplify GC-rich templates with the GC-RICH PCR System. Amplification of a 264 bp template (74% GC content) within the human ApoE gene using the GC-RICH PCR System or the Expand High Fidelity PCR System.

Result: The GC-RICH PCR System amplifies the GC-rich fragment with high specificity and yield using GC-RICH Resolution Solution.

 

Amplicon Size Specificity Sensitivity Robustness Accuracy
vs. Taq
Carryover Prevention Units
/50 µL
Molecular Cloning
Up to 5 kb 3x no 2 TA cloning preferred over blunt end cloning

 

Product SKU Pack Size
GC-RICH PCR System | Enzyme + Buff
  12 140 306 001
100 U for up to 50 reactions of 50 μL final volume, each containing 2 U enzyme blend
GC-RICH PCR System, dNTPack | Enzyme + Buffer + dNTP Set
  04 743 784 001 100 U for up to 50 reactions of 50 μL final volume, each containing 2 U enzyme blend

Expand™ Long Range, dNTPack

Rely on this next-generation system from Roche, the company that pioneered long-template PCR. Choose Expand™ Long Range dNTPack for consistent amplification of PCR products of 5 to 25 kb from genomic DNA. When amplifying fragments longer than 20 kb, use Expand™ 20 kbPLUS PCR System. This unique system features an optimized buffer and enzyme blend mixture.

  • Consistently amplify long templates up to 25 kb with high specificity, yield, and increased fidelity.
  • Save time and resources with a convenient and flexible kit. Use one buffer for all fragment sizes and use the supplied DMSO and MgClsolution to fine-tune your reaction.
  • Use for all long-template applications, obtaining high yields of only the desired fragment.
  • Order the cost-effective dNTPack which also includes premixed PCR-Grade nucleotides.

Amplification of various genomic templates using Expand Long Range, dNTPack.

Figure 9. Amplification of various genomic templates using Expand™ Long Range, dNTPack

 

Amplicon Size Specificity Sensitivity Robustness Accuracy
vs. Taq
Carryover Prevention Units
/50 µL
Molecular Cloning
5 to 25 kb 3x no 3.5 TA cloning

 

Product SKU Pack Size
Expand™ Long Range, dNTPack | Enzyme + Buffer + dNTP Set
  04 829 034 001 175 U for up to 125 reactions of 20 μL final volume, each containing 1.4 U enzyme blend
04 829 042 001 700 U for up to 500 reactions of 20 μL final volume, each containing 1.4 U enzyme blend
04 829 069 001 3,500 U (5 × 700 U) for up to 2,500 reactions of 20 μL final volume, each containing 1.4 U enzyme blend

 

* For amplicon sizes of 20-35 kb: choose Expand 20 kbPLUS PCR System.

Product SKU Pack Size
Expand™ 20 kbPLUS PCR System | Enzyme + Buffer
  11 811 002 001 200 U for up to 40 reactions of 50 μL final volume, each containing 5 U enzyme blend

 

Products for PCR Optimization

Product SKU Pack Size
PCR Optimization Kit 11 636 138 001 1 kit for up to 100 one-step or up to 50 two-step optimization assays of 100 μL final reaction volume
Uracil-DNA Glycosylase 11 444 646 001 100 U
Uracil-DNA Glycosylase, heat-labile 11 775 367 001 100 U
11 775 375 001 500 U
T4 Gene 32 Protein 10 972 983 001 100 µg
10 972 991 001 500 µg
PCR Buffer Set 11 699 121 001 2 x 2 mL (2 × 1 mL of each solution)
PCR Buffer without MgCl2 10x conc 11 699 105 001 3 × 1 mL
MgCl2 Stock Solution 11 699 113 001 3 × 1 mL
Water, PCR Grade 03 315 843 001 100 mL (4 vials of 25 mL)
03 315 932 001 25 mL (25 vials of 1 mL)
03 315 959 001 25 ml (1 vial of 25 mL)
Strip PCR Tubes and Caps 11 667 009 001 125 strips (8 tubes/strip)