SciFlow™ 1000 Fluidic Culture System

Enabling Human Physiology Research on the Benchtop

SciFlow™ 1000 Fluidic Culture SystemThe SciFlow™ 1000 Fluidic Culture System is the first 96-well format in vitro fluidics culture system to seamlessly integrate with your laboratory workflow. This system enables you to establish gradients between cell culture wells and over time, serially connect multiple organ systems, and monitor downstream metabolite and bioactivation effects. The SciFlow™ System is designed to mimic in vivo-like conditions, enabling the evaluation of your compound’s effects in a human biology relevant manner.

Benefits of the SciFlow™ System:

  • Cell Health and Viability: Cells cultured in a fluidic environment are healthier and show viability for extended periods of time. Dynamic flow creates a more in vivo-like environment.
  • Gradient Exposure: Simultaneously interrogate multiple compound concentrations during the course of the experiment.
  • Parent / Metabolite / Discrimination: Open access to all cellular compartments enables identification and quantification of all metabolic products.
  • Multi-Organ Capabilities: Different cells and tissues can be cultured in interconnected wells, enabling metabolites and conditioned media to flow downstream for more biologically relevant compound exposure data.
  • Broad Compatibility: The system is ideal for culturing, exposing, and analyzing cells and is compatible with plate readers, high-content imagers, multi-channel pipettes, and a array of laboratory automation equipment.
  • More accurate assessment of adaptive vs toxic mechanisms.
  • The SciFlow™ system is mass produced for product repeatability (injection molded), using traditional polystyrene material that limits non-specific binding:  no need to re-qualify your cell or compound activities.
  • Evaluate real-time exposures that range from 0, to sub-toxic gradients, to lethal toxic levels.
  • Open access to all cellular compartments allows for identification and quantification of all metabolic products.

SciFlow™ 1000 Fluidic Culture System wells

Imaging

Standard Inversion Microscopy
Microscopy
Standard Inversion Microscopy
high content imaging
High Content Imaging
Hoechst Stain - Blue
CellTox Green - Pink

 

SciKon Innovation, Inc. is a life sciences company that supports the pharmaceutical, biotech, agricultural, environmental and chemical industries by delivering innovative tools, products and services for improving research operations. SciKon provides these industries with research tools and services, including the SciFlow™ 1000 Fluidic Culture System, specialty media, contract research services, tissue and skin procurement services, as well as custom research tool development.

Frequently Asked Questions

  1. Is the SciFlow™ system tissue treated for cell attachment?
    Yes.

  2. Are the SciFlow™ system wells in the same location as a standard 96 well plate?
    The SciFlow™ plate was designed with 96-well culture locations. “x” and “y” well centroids are maintained for wells 2 – 11 according to ANSI standards.

  3. How does the SciFlow™ system differ in z-height?
    Each well in a row is offset 0.5 mm from the one next to it.

  4. What is the surface area of each culture well?
    To include micro-channels within the SciFlow™ system, the area of cultures wells have been reduced by ½ of a traditional 96-well plate (i.e., use ½ the cells per surface area). The surface area is 16.7 mm2.

  5. Why do I need to pre-incubate the system in humidity before using?
    Humidified air aids the surface wettability of the plate. Bypassing the pre-humidification step plate can reduce the ability to connect channels and can cause the initial media deposition to aberrantly enter the capillary channels rather than spreading in the wells.

  6. How do I know what concentration my drug is at any given time?
    Fluorescein dye is relatively non-toxic at concentrations <1 μM. There are two ways to use fluorescein to extrapolate your drug/toxicant concentration: A) If possible, use 1 μM Fluorescein with your chemical or drug and perform regular plate-reading to track the fluid. Drug/Toxicant concentrations can be extrapolated using a standard curve for Fluorescein from 1 μM to 10 pM. B) If it’s not possible to use Fluorescein because of toxicity and/or use a fluorescent assay in the same ex/em range as fluorescein, we recommend dedicating one or two rows of each SciFlow™ system specifically for a fluorescein control.

  7. Can I use the SciFlow™ system in my plate reader?
    SciKon has tested several plate readers for compatibility. Typically, plate readers introduced in the last 3-5 years are highly compatible. However, we are unable to test all plate readers. To see if your plate reader might be compatible, examine the software and hardware specifications for two key features.

  8. What is the capacity of the SciFlow™ system in a plate reader?
    The height of the SciFlow™ plate is 17.6 mm (without lid), which is about 3.5 mm more than a standard 96-well plate. The height of the SciFlow™ plate with lid is 20.5 mm. If a plate reader can accept 24 well plates, it will accept SciFlow. Talk to the manufacturer to determine if your instrument can accept the SciFlow™ plate.

  9. Can I do imaging experiments with the SciFlow™ system?
    Yes. If you can adjust focal heights either manually or by automation through a 4 mm range, then you should be able to image each well of the SciFlow™ plate (well 3 – 11). Check with your instrument manufacturer to determine appropriate settings.

  10. Do you make the SciFlow™ system with black side-walls for preventing fluorescence cross-talk?
    The SciFlow™ plate was designed with thick and impervious sidewalls and a small air-gap between the adjacent well walls. These features render the need for black side walls unnecessary.

  11. Why is there a flat surface on part of the wells rather than being completely circular?
    The shape of the well was established to enable better mixing dynamics and by manufacture constraints.

Materials

     
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