Transfection Reagent Selection Guide

Choosing a transfection reagent is easier when you can rely on the work of researchers who have used it before you. During development of each reagent, good transfection efficiency was verified in a variety of cells (as indicated in the product manual). Scientific publications continue to add to the growing list of cells that are transfected well with our transfection reagents. You can view these publications from the links on the product pages. Use the chart below to determine which reagent is right for your cells. For more help on choosing a reagent, review our Introduction to Transfection.

See the Roche X-tremeGENE™ Transfection Database for hundreds more protocols and articles.

 


  = excellent       = good/compatible

Reagent Escort™ III Transfection Reagent
(L3037)
Escort™ IV Transfection Reagent
(L3287)
Calcium Phosphate Transfection Kit
(CAPHOS)
DOTAP
Liposomal Transfection Reagent
(11202375001)
NeuroPorter™ Transfection
Kit
(NPT01)
General Guidelines Primary cells, sensitive cells Insect cells, mammalian cell lines, some primary cells Common cell lines Common cell lines Neuronal cell lines, glial cells, some primary neurons
Mammalian Cell Lines
16HBE14o-          
3LL          
6CSFMEo          
A549      
B16-F10          
BAEC        
BHK-21        
C2C12      
Ca Ski          
CHO
(see image)
     
CHOK1    
COS
(see image)
       
COS-1      
COS-7    
CV-1          
D 407          
DHD Pro.b          
H9c2        
HaCaT        
HEK293    
HeLa  
HepG2      
IMR90        
Jurkat        
L929          
LNCaP        
MCF-7      
MDA-MB-435        
MDCK        
Neuro2A          
NIH3T3  
PC-12
(see image)
     
SAOS-2        
SHEP          
SH-SY5Y        
SiHa          
SKOV3          
T-24        
Vero      
WI-38      
Insect Cell Lines
Sf9 (adherent)      
Sf9 (suspension)        
S2 (D. mel.)
(see image)
       
S2R+        
T. Ni.      
Primary Cells
Aortic smooth muscle cells
(rat)
       
Blastocyst (mouse)        
Cardiac myocytes (human)        
Cardiomyocytes (rat neonatal)        
Cardiomyocytes (rat)        
Fibroblasts
( human skin)
         
Fibroblasts
(rat)
       
Germ cells
(male rat)
       
Hepatocytes (hampster)        
Hepatocytes
(rat)
       
HLMVEC          
H-MVEC          
HUVEC        
Keratinocytes (human)
(see image)
     
Myoblasts (rat)        
Myoblasts (quail)
(see image)
     
Oviduct cells (bovine)          
Pre-adipocytes (human)          
Retinal neurons (chick)        
Tracheobronchial cells (human)        
Neuronal & Glial Cells
Astrocytes
(see image)
       
C6 Glioma        
Hippocampal neurons
(primary rat)
       
NT2        
NT2 (differentiated neurons)        

 

Reagent GeneJuice® Transfection Reagent (70967) 293-Free™ Transfection Reagent (72181) NovaCHOice® Transfection Kit (72622-M) RiboJuice™ siRNA Transfection Reagent (71115) Insect GeneJuice® Transfection Reagent (71259) Polybrene Infection / Transfection Reagent (TR-1003)
General guidelines Non-lipid based chemical transfection reagent optimized for maximum transfection efficiency, ease-of-use, and minimal cytotoxicity on a wide variety of mammallian cells. Animal-free polycationic liposomal transfection reagent optimized for the transfection of HEK293 cells grown in suspension culture. Optimized transfection reagent specifically developed for mammalian protein production in chinese hamster ovary (CHO) cells. Optimized, easy-to-use siRNA transfection reagent formulated for minimal toxicity. A proprietary liposome formulation optimized for maximal transfection efficiency of Sf9 insect cells. A highly efficient method of gene transfer into mammalian cells leveraging infection with retroviral vectors.
L428          
L591          
Neuro-2A          
10T1/2            
293T    
3T3 NIH      
3T3 Swiss            
3T3-L1            
A204            
A431            
A549        
alpha TC1-6            
AR 42J            
As4.1            
AtT-20            
B50            
BC-1            
BC-2            
BC3            
BCBL            
BHK-21            
C3H/10T1/2            
C6            
C2C12            
Caki-1            
Calpan-1            
Calu-1            
Calu-6            
CCL-131            
CFPAC-1            
Chang Liver            
CHO    
CHO-7    
CHO-IR    
CHO-K1    
COS-1            
COS-7        
CS-1            
CV-1            
Daudi            
DDTI MF-2            
DT40            
ECV304            
EL4            
EVSCC17M            
H9c2            
HCT-116            
HEK293            
HeLa        
HeLa B        
HeLa T4        
Hep 3B2.1-7            
HepG2        
Hepa 1-6            
Ht-29            
HTB-37            
HTB-45            
Huh-7            
HUVEC            
IC21            
IEC-6            
JEG-3            
Jurkat            
KB            
L-6            
L-929            
MA-10            
McA-RH7777            
MCF-7        
MCF-10-2A            
MDCK            
Melanocyte            
MG-63            
Neuro 2A            
Neuroblastoma            
NRK            
NT2/D1            
OV-1063            
OVCAR3            
P4            
P19            
PC12            
PA317            
PAM212            
R2C            
RAW 264.7            
RBL-2H3            
RMP-41            
SAOS-2            
SC-1            
Schneider line2            
SK-N-MC            
SK-N-SH            
SKOV3            
STO            
SW-480            
SW-837            
T3M4            
TM4            
U937            
UCD            
Vero            
   
Sf9 (adherent)          
Sf9 (suspension)          
S2 (D. mel.)            
S2R+            
T. Ni.            
   
rat HSC          
Chicken embryo fibroblases          
Myeloid cells          
Aortic smooth muscle cells    
Astrocytes            
Angioblasts            
Chondrocytes            
Chromaffin cells            
Epithelial cells:            
  mammary            
  prostate            
  tracheal            
Fibroblasts            
Keratinocytes          

 

Find more protocols and selection guides in the Molecular Biology Guide.

Materials