Transfection Reagent Selection Guide

Choosing a transfection reagent is easier when you can rely on the work of researchers who have used it before you. During development of each reagent, good transfection efficiency was verified in a variety of cells (as indicated in the product manual). Scientific publications continue to add to the growing list of cells that are transfected well with our transfection reagents. You can view these publications from the links on the product pages. Use the chart below to determine which reagent is right for your cells. For more help on choosing a reagent, review our Introduction to Transfection.

See the Roche X-tremeGENE™ Transfection Database for hundreds more protocols and articles.

 


  = excellent       = good/compatible

Reagent Escort™ III Transfection Reagent
(L3037)
Escort™ IV Transfection Reagent
(L3287)
Calcium Phosphate Transfection Kit
(CAPHOS)
DOTAP
Liposomal Transfection Reagent
(11202375001)
NeuroPorter™ Transfection
Kit
(NPT01)
General Guidelines Primary cells, sensitive cells Insect cells, mammalian cell lines, some primary cells Common cell lines Common cell lines Neuronal cell lines, glial cells, some primary neurons
Mammalian Cell Lines
16HBE14o-          
3LL          
6CSFMEo          
A549      
B16-F10          
BAEC        
BHK-21        
C2C12      
Ca Ski          
CHO
(see image)
     
CHOK1    
COS
(see image)
       
COS-1      
COS-7    
CV-1          
D 407          
DHD Pro.b          
H9c2        
HaCaT        
HEK293    
HeLa  
HepG2      
IMR90        
Jurkat        
L929          
LNCaP        
MCF-7      
MDA-MB-435        
MDCK        
Neuro2A          
NIH3T3  
PC-12
(see image)
     
SAOS-2        
SHEP          
SH-SY5Y        
SiHa          
SKOV3          
T-24        
Vero      
WI-38      
Insect Cell Lines
Sf9 (adherent)      
Sf9 (suspension)        
S2 (D. mel.)
(see image)
       
S2R+        
T. Ni.      
Primary Cells
Aortic smooth muscle cells
(rat)
       
Blastocyst (mouse)        
Cardiac myocytes (human)        
Cardiomyocytes (rat neonatal)        
Cardiomyocytes (rat)        
Fibroblasts
( human skin)
         
Fibroblasts
(rat)
       
Germ cells
(male rat)
       
Hepatocytes (hampster)        
Hepatocytes
(rat)
       
HLMVEC          
H-MVEC          
HUVEC        
Keratinocytes (human)
(see image)
     
Myoblasts (rat)        
Myoblasts (quail)
(see image)
     
Oviduct cells (bovine)          
Pre-adipocytes (human)          
Retinal neurons (chick)        
Tracheobronchial cells (human)        
Neuronal & Glial Cells
Astrocytes
(see image)
       
C6 Glioma        
Hippocampal neurons
(primary rat)
       
NT2        
NT2 (differentiated neurons)        

 

Reagent GeneJuice® Transfection Reagent (70967) 293-Free™ Transfection Reagent (72181) NovaCHOice® Transfection Kit (72622-M) RiboJuice™ siRNA Transfection Reagent (71115) Insect GeneJuice® Transfection Reagent (71259) Polybrene Infection / Transfection Reagent (TR-1003)
General guidelines Non-lipid based chemical transfection reagent optimized for maximum transfection efficiency, ease-of-use, and minimal cytotoxicity on a wide variety of mammallian cells. Animal-free polycationic liposomal transfection reagent optimized for the transfection of HEK293 cells grown in suspension culture. Optimized transfection reagent specifically developed for mammalian protein production in chinese hamster ovary (CHO) cells. Optimized, easy-to-use siRNA transfection reagent formulated for minimal toxicity. A proprietary liposome formulation optimized for maximal transfection efficiency of Sf9 insect cells. A highly efficient method of gene transfer into mammalian cells leveraging infection with retroviral vectors.
Mammalian cell lines
  L428        
L591        
Neuro-2A        
  10T1/2          
  293T  
  3T3 NIH    
  3T3 Swiss          
  3T3-L1          
  A204          
  A431          
  A549      
  alpha TC1-6          
  AR 42J          
  As4.1          
  AtT-20          
  B50          
  BC-1          
  BC-2          
  BC3          
  BCBL          
  BHK-21          
  C3H/10T1/2          
  C6          
  C2C12          
  Caki-1          
  Calpan-1          
  Calu-1          
  Calu-6          
  CCL-131          
  CFPAC-1          
  Chang Liver          
  CHO  
  CHO-7  
  CHO-IR  
  CHO-K1  
  COS-1          
  COS-7      
  CS-1          
  CV-1          
  Daudi          
  DDTI MF-2          
  DT40          
  ECV304          
  EL4          
  EVSCC17M          
  H9c2          
  HCT-116          
  HEK293          
  HeLa      
  HeLa B      
  HeLa T4      
  Hep 3B2.1-7          
  HepG2      
  Hepa 1-6          
  Ht-29          
  HTB-37          
  HTB-45          
  Huh-7          
  HUVEC          
  IC21          
  IEC-6          
  JEG-3          
  Jurkat          
  KB          
  L-6          
  L-929          
  MA-10          
  McA-RH7777          
  MCF-7      
  MCF-10-2A          
  MDCK          
  Melanocyte          
  MG-63          
  Neuro 2A          
  Neuroblastoma          
  NRK          
  NT2/D1          
  OV-1063          
  OVCAR3          
  P4          
  P19          
  PC12          
  PA317          
  PAM212          
  R2C          
  RAW 264.7          
  RBL-2H3          
  RMP-41          
  SAOS-2          
  SC-1          
  Schneider line2          
  SK-N-MC          
  SK-N-SH          
  SKOV3          
  STO          
  SW-480          
  SW-837          
  T3M4          
  TM4          
  U937          
  UCD          
  Vero          
Insect Cell Lines
  Sf9 (adherent)        
  Sf9 (suspension)        
  S2 (D. mel.)          
  S2R+          
  T. Ni.          
Primary Cells
  rat HSC        
Chicken embryo fibroblases        
Myeloid cells        
  Aortic smooth muscle cells  
  Astrocytes          
  Angioblasts          
  Chondrocytes          
  Chromaffin cells          
  Epithelial cells:          
    mammary          
    prostate          
    tracheal          
  Fibroblasts          
  Keratinocytes        

 

Find more protocols and selection guides in the Molecular Biology Guide.

Materials