Trending Papers at Sigma-Aldrich.com



February 2017

Chimera animal models advanced by using CRISPR

Chimera animals have been created to both study drug effects in humans and also the goal of creating organs for use in humans.  A recent breakthrough using CRISPR has occurred to knockout an animal fetus’ immune response to human cells.  Now that the human cells can flourish in a non-human fetus, multiple human organs were grown in Chimera Pigs and Cows.  As CRISPR tools become mastered, organs grown in animals will eventually be used to save human lives.

Wu Jun, Platero-Luengo Aida, Sakurai Masahiro, Sugawara Atsushi, Gil MariaAntonia, Yamauchi Takayoshi, Suzuki Keiichiro, Bogliotti YaninaSoledad, Cuello Cristina, MoralesValencia Mariana, Okumura Daiji, Luo Jingping, Vilario Marcela, Parrilla Inmaculada, Soto DeliaAlba, Martinez CristinaA, Hishida Tomoaki, Snchez-Bautista Sonia, Martinez-Martinez MLlanos, Wang Huili, Nohalez Alicia, Aizawa Emi, Martinez-Redondo Paloma, Ocampo Alejandro, Reddy Pradeep, Roca Jordi, Maga ElizabethA, Esteban ConcepcionRodriguez, Berggren WTravis, NuezDelicado Estrella, Lajara Jeronimo, Guillen Isabel, Guillen Pedro, Campistol JosepM, Martinez EmilioA, Ross PabloJuan, IzpisuaBelmonte JuanCarlos
Cell 2017-01-01
Interspecies blastocyst complementation enables organ-specific enrichment of xenogenic pluripotent stem cell (PSC) derivatives. Here, we establish a versatile blastocyst complementation platform based on CRISPR-Cas9-mediated zygote genome editing and show enrichment of rat PSC-derivatives in several tissues of gene-edited organo...Read More

Product Numbers

Anti-Actin Antibody

Cas9 mRNA

Hot Papers

Here are 5 of the most trending papers on Sigma-Aldrich.com in January. These hot papers have helped our customers further their research.  Sigma-Aldrich provides both a wide array of life-science and chemical products along with a wide-array of peer-reviewed papers.

Kevser Sözgen Başkan, Esma Tütem, Esin Akyüz, Seda Özen, Reşat Apak
Talanta 2016-01-15
As the concentration of reducing sugars (RS) is controlled by European legislation for certain specific food and beverages, a simple and sensitive spectrophotometric method for the determination of RS in various food products is proposed. The method is based on the reduction of Cu(II) to Cu(I) with reducing sugars in alkaline me...Read More
Rebecca C Coll, Avril A B Robertson, Jae Jin Chae, Sarah C Higgins, Raúl Muñoz-Planillo, Marco C Inserra, Irina Vetter, Lara S Dungan, Brian G Monks, Andrea Stutz, Daniel E Croker, Mark S Butler, Moritz Haneklaus, Caroline E Sutton, Gabriel Núñez, Eicke Latz, Daniel L Kastner, Kingston H G Mills, Seth L Masters, Kate Schroder, Matthew A Cooper, Luke A J O'Neill
Nature Medicine 2015-03-01
The NOD-like receptor (NLR) family, pyrin domain-containing protein 3 (NLRP3) inflammasome is a component of the inflammatory process, and its aberrant activation is pathogenic in inherited disorders such as cryopyrin-associated periodic syndrome (CAPS) and complex diseases such as multiple sclerosis, type 2 diabetes, Alzheimer'...Read More
Matthew W Bernart
Journal of AOAC International 2015-01-01
The citrus-derived bioactive monoterpene limonene is an important industrial commodity and fragrance constituent. An RP isocratic elution C18 ultra-HPLC (UHPLC) method using a superficially porous stationary phase and photodiode array (PDA) detector has been developed for determining the limonene content of sweet orange (Citrus ...Read More
Nathan P Young, Anwesh Kamireddy, Jeanine L Van Nostrand, Lillian J Eichner, Maxim Nikolaievich Shokhirev, Yelena Dayn, Reuben J Shaw
Genes & Development 2016-03-01
Faithful execution of developmental programs relies on the acquisition of unique cell identities from pluripotent progenitors, a process governed by combinatorial inputs from numerous signaling cascades that ultimately dictate lineage-specific transcriptional outputs. Despite growing evidence that metabolism is integrated with m...Read More
Ewa Grela, Adam Ząbek, Agnieszka Grabowiecka
Avicenna Journal of Medical Biotechnology 2015-01-01
The chromogenic assay based on MTT bioreduction was adapted to Proteus mirabilis viability estimations. We primarily intended to use the assay for the evaluation of novel antimicrobial compounds, including structures with possible permeabilizing activity. Therefore, the influence of basic permeabilizing agents like Triton X-100 ...Read More

December 2016

Membrane protein from Akkermansia munciniphila found to improve metabolism

Recent studies have been focused on the relationship between obesity and a person’s gut biota. A study has found that a membrane protein from Akkermansia munciniphila can improve the metabolism of obese mice. This was a direct study showing that metabolism can be affected by introducing only a part of a bacterium not currently in the gut biota. Additionally, pasteurizing a culture of A. munciniphila and introducing it to the mice further increased their metabolism. This study shows that the next step after discovering an important bacterium related to obesity is to study how it affects it so much.

Plovier H, Everard A, Druart C, Depommier C, Van Hul M, Geurts L, Chilloux J, Ottman N, Duparc T, Lichtenstein L, Myridakis A, Delzenne NM, Klievink J, Bhattacharjee A, van der Ark KC, Aalvink S, Martinez LO, Dumas ME, Maiter D, Loumaye A, Hermans MP, Thissen JP, Belzer C, de Vos WM, Cani PD
Nature Medicine 2016-11-01
Obesity and type 2 diabetes are associated with low-grade inflammation and specific changes in gut microbiota composition. We previously demonstrated that administration of Akkermansia muciniphila to mice prevents the development of obesity and associated complications. However, the underlying mechanisms of this protective effec...Read More

Hot Papers

Here are 5 of the most trending papers on Sigma-Aldrich.com in November. These hot papers have helped our customers further their research.  Sigma-Aldrich provides both a wide array of life-science and chemical products along with a wide-array of peer-reviewed papers.

Hui Zhao, Jianxin Wang, Xiaodan Kong, Encheng Li, Yuanbin Liu, Xiaohui Du, Zhijie Kang, Ying Tang, Yanbin Kuang, Zhihui Yang, Youwen Zhou, Qi Wang
Scientific reports 2016-01-01
CD47 is overexpressed in many human cancers, its level positively correlates with tumor invasion and metastasis. However, it is largely unknown whether CD47 overexpression drives metastasis and how CD47 lead to tumor metastasis in non-small cell lung cancer (NSCLC). In this study, we analyzed NSCLC specimens and cell lines, and ...Read More
Juan P Zúñiga-Hertz, Eduardo Rebelato, Adam Kassan, Abdelrahman M Khalifa, Sameh S Ali, Hemal H Patel, Fernando Abdulkader
Journal of Endocrinology 2015-01-01
Results from previous investigations have indicated that glucose-stimulated insulin secretion (GSIS) is affected by changes in cholesterol and its intermediates, but the precise link between secretion and cholesterol has not been thoroughly investigated. In this study, we show the contribution of both protein isoprenylation and ...Read More
Elisabeth Corcelle-Termeau, Signe Diness Vindeløv, Saara Hämälistö, Baharia Mograbi, Anne Keldsbo, Jan Hinrich Bräsen, Elena Favaro, Dieter Adam, Piotr Szyniarowski, Paul Hofman, Stefan Krautwald, Thomas Farkas, Nikolaj H T Petersen, Mikkel Rohde, Andreas Linkermann, Marja Jäättelä
Autophagy 2016-05-03
Sphingomyelin is an essential cellular lipid that traffics between plasma membrane and intracellular organelles until directed to lysosomes for SMPD1 (sphingomyelin phosphodiesterase 1)-mediated degradation. Inactivating mutations in the SMPD1 gene result in Niemann-Pick diseases type A and B characterized by sphingomyelin accum...Read More
James P K Armstrong, Madeline Burke, Benjamin M Carter, Sean A Davis, Adam W Perriman
Advanced Healthcare Materials 2016-07-01
3D tissue printing with adult stem cells is reported. A novel cell-containing multicomponent bioink is used in a two-step 3D printing process to engineer bone and cartilage architectures. © 2016 The Authors. Published by WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.Read More
Nan Jiang, Guiqiu Zhao, Jing Lin, Liting Hu, Chengye Che, Cui Li, Qian Wang, Qiang Xu, Xudong Peng
PLoS ONE 2015-01-01
Indoleamine 2,3-dioxygenase (IDO), which is mainly expressed in activated dendritic cells, is known as a regulator of immune responses. However, the role of IDO in immune responses against fungal corneal infection has not been investigated. To evaluate the regulatory mechanisms of IDO in fungal inflammation, we resorted to human...Read More

November 2016

New Bioactive Small Molecule S63845 found to treat a large number of cancers

The elimination of apoptosis is how cancer cells can proliferate and spread. A common anti-apoptosis protein that cancer cells overexpress is myeloid cell leukemia 1 (MCL1). S63845 has been found to have a high affinity towards MCL1, so much so that MCL1 activity is blocked and cancer cells can go through apoptosis. It is also believed that S63845 doses can be devised so that it would not affect healthy cells, only cancer cells. With this discovery it is hopeful that a bioactive small molecule could be used to treat a wide range of cancers instead of going through the difficult process of Radiation or Chemotherapy.

Andrs Kotschy, Zoltn Szlavik, James Murray, James Davidson, AnaLeticia Maragno, Gatane LeToumelin-Braizat, Maa Chanrion, GemmaL Kelly, Jia-Nan Gong, DoniaM Moujalled, Alain Bruno, Mrton Csekei, Attila Paczal, ZoltnB Szabo, Szabolcs Sipos, Gbor Radics, Agnes Proszenyak, Balzs Balint, Levente Ondi, Gbor Blasko, Alan Robertson, Allan Surgenor, Pawel Dokurno, Ijen Chen, Natalia Matassova, Julia Smith, Christopher Pedder, Christopher Graham, Aurlie Studeny, Galle Lysiak-Auvity, Anne-Marie Girard, Fabienne Grav, David Segal, ChrisD Riffkin, Giovanna Pomilio, LauraCA Galbraith, BrandonJ Aubrey, MargsS Brennan, MarcoJ Herold, Catherine Chang, Ghislaine Guasconi, Nicolas Cauquil, Fabien Melchiore, Nolwen Guigal-Stephan, Brian Lockhart, Frdric Colland, JohnA Hickman, AndrewW Roberts, DavidCS Huang, AndrewH Wei, Andreas Strasser, Guillaume Lessene, and Olivier Geneste
Nature 538(7626):477 (2016)
Avoidance of apoptosis is critical for the development and sustained growth of tumours. The pro-survival protein myeloid cell leukemia 1 (MCL1) is overexpressed in many cancers, but the development of small molecules targeting this protein that are amenable for clinical testing has been challengi...Read More

Hot Papers

Here are 5 of the most trending papers on Sigma-Aldrich.com in October. These hot papers have helped our customers further their research.  Sigma-Aldrich provides both a wide array of life-science and chemical products along with a wide-array of peer-reviewed papers.

Ran Kang, Yan Zhou, Shuang Tan, Guangqian Zhou, Lars Aagaard, Lin Xie, Cody Bünger, Lars Bolund, Yonglun Luo
Stem Cell Research & Therapy 2015-01-01
Previously, we established a simple method for deriving mesenchymal stem cells (MSCs) from human induced pluripotent stem cells (iPSC-MSCs). These iPSC-MSCs were capable of forming osteogenic structures in scaffolds and nanofibers. The objective of this study is to systematically characterize the mesenchymal characteristics of t...Read More
Piyarat Weerachanchai, Yuewen Wong, Kok Hwa Lim, Timothy Thatt Yang Tan, Jong-Min Lee
ChemPhysChem 2014-11-10
The total and partial solubility parameters (dispersion, polar and hydrogen-bonding solubility parameters) of ten ionic liquids were determined. Intrinsic viscosity approaches were used that encompassed a one-dimensional method (1D-Method), and two different three-dimensional methods (3D-Method1 and 3D-Method2). The effect of so...Read More
Fatemeh Ganji, Saeid Abroun, Hossein Baharvand, Nasser Aghdami, Marzieh Ebrahimi
Cell Journal (Yakhteh) 2015-01-01
There is constant difficulty in obtaining adequate supplies of blood components, as well as disappointing performance of "universal" red blood cells. Advances in somatic cell reprogramming of human-induced pluripotent stem cells (hiPSCs) have provided a valuable alternative source to differentiate into any desired cell type as a...Read More
Simon Stritt, Paquita Nurden, Remi Favier, Marie Favier, Silvia Ferioli, Sanjeev K Gotru, Judith M M van Eeuwijk, Harald Schulze, Alan T Nurden, Michele P Lambert, Ernest Turro, Stephanie Burger-Stritt, Masayuki Matsushita, Lorenz Mittermeier, Paola Ballerini, Susanna Zierler, Michael A Laffan, Vladimir Chubanov, Thomas Gudermann, Bernhard Nieswandt, Attila Braun
Nature Communications 2016-01-01
Mg(2+) plays a vital role in platelet function, but despite implications for life-threatening conditions such as stroke or myocardial infarction, the mechanisms controlling [Mg(2+)]i in megakaryocytes (MKs) and platelets are largely unknown. Transient receptor potential melastatin-like 7 channel (TRPM7) is a ubiquitous, constitu...Read More
Eleonora Franzè, Ivan Monteleone, Maria Laura Cupi, Pamela Mancia, Flavio Caprioli, Irene Marafini, Alfredo Colantoni, Angela Ortenzi, Federica Laudisi, Giuseppe Sica, PierPaolo Sileri, Francesco Pallone, Giovanni Monteleone
Clinical Science 2015-08-01
IBD (inflammatory bowel disease)-related tissue damage occurs in areas which are massively infiltrated with monocytes/macrophages. These cells respond to inflammatory stimuli with enhanced production of cytokines/chemokines. In the present study, we analysed the expression and role of IL (interleukin)-34, a regulator of monocyte...Read More

October 2016

Microbiome found to be cause of Crohn’s disease

The complex interaction between a person’s genome and their microbiome has been shown to be a cause of Crohn’s disease. The Microbiome factor of the disease is becoming more and more understood as different types of treatments are discovered. A recent study found that the interplay of C. tropicalis, S. marcescens, and E. coli point towards the cause of Crohn’s in some patients. What’s interesting about those organisms is that two are bacteria while C. tropicalis is a yeast. As we learn more about the complex nature of Crohn’s disease, better and more permanent treatments will be discovered.

G Hoarau, PK Mukherjee, C Gower-Rousseau, C Hager, J Chandra, MA Retuerto, C Neut, S Vermeire, J Clemente, JF Colombel, H Fujioka, D Poulain, B Sendid, MA Ghannoum
mBio 2016-09-01
Crohn's disease (CD) results from a complex interplay between host genetic factors and endogenous microbial communities. In the current study, we used Ion Torrent sequencing to characterize the gut bacterial microbiota (bacteriome) and fungal community (mycobiome) in patients with CD and their nondiseased first-degree relatives ...Read More

Hot Papers

Here are 5 of the most trending papers on Sigma-Aldrich.com in September. These hot papers have helped our customers further their research.  Sigma-Aldrich provides both a wide array of life-science and chemical products along with a wide-array of peer-reviewed papers.

P Haro-González, L Martínez-Maestro, I R Martín, J García-Solé, D Jaque
Small 2012-09-10
The potential use of CdTe quantum dots as luminescence nano-probes for lifetime fluorescence nano-thermometry is demonstrated. The maximum thermal sensitivity achievable is strongly dependent on the quantum dot size. For the smallest sizes (close to 1 nm) the lifetime thermal sensitivity overcomes those of conventional nano-prob...Read More
Yi Lan, Hui Li, Yan-yan Chen, Ye-wen Zhang, Na Liu, Qing Zhang, Qing Wu
Journal of Zhejiang University (Science B) 2014-11-01
Our previous studies had confirmed that the essential oil from Zanthoxylum bungeanum Maxim. (Z. bungeanum oil) could effectively enhance the percutaneous permeation of drug molecules as a natural transdermal penetration enhancer. The aim of the present study is to investigate and compare the skin penetration enhancement effect o...Read More
Jennifer Jung, Heide Marika Genau, Christian Behrends
Molecular and Cellular Biology 2015-07-01
The serine/threonine kinase mTORC1 regulates cellular homeostasis in response to many cues, such as nutrient status and energy level. Amino acids induce mTORC1 activation on lysosomes via the small Rag GTPases and the Ragulator complex, thereby controlling protein translation and cell growth. Here, we identify the human 11-pass ...Read More
Fabian Müller, Constanza A Pontones, Bertold Renner, Maren Mieth, Eva Hoier, Daniel Auge, Renke Maas, Oliver Zolk, Martin F Fromm
European Journal of Clinical Pharmacology 2015-01-01
N(1)-methylnicotinamide (NMN) was proposed as an in vivo probe for drug interactions involving renal cation transporters, which, for example, transport the oral antidiabetic drug metformin, based on a study with the inhibitor pyrimethamine. The role of NMN for predicting other interactions with involvement of renal cation transp...Read More
Gerard Ja Rouwendal, Miranda M van der Lee, Saskia Meyer, Karli R Reiding, Jan Schouten, Guy de Roo, David F Egging, Jeanette Hw Leusen, Peter Boross, Manfred Wuhrer, Gijs F Verheijden, Wim H Dokter, Marco Timmers, Ruud Ubink
mAbs 2016-01-01
Monomeric IgA has been proposed as an alternative antibody format for cancer therapy. Here, we present our studies on the production, purification and functional evaluation of anti-HER2 IgA antibodies as anti-cancer agents in comparison to the anti-HER2 IgG1 trastuzumab. MALDI-TOF MS analysis showed profound differences in glyco...Read More

September 2016

New Antibody found to be effective against Alzheimer’s Plaques

A recent published study found that the antibody aducanumab is effective in removing Beta Amyloid Plaques from transgenic mice.  The antibody was also found to subsequently improve memory function in the mice.  Unlike many previous studies, this study went further in the fact that it includes initial findings from a human drug trial with the same antibody.  Early findings in humans have been promising, but the phase 3 FDA trial is not yet complete.  Although there were failed Alzheimer’s phase 3 trials in the past, continued studies show that Alzheimer’s may become a manageable disease with the correct treatment.

Jeff Sevigny, Ping Chiao, Thierry Bussire, PaulH Weinreb, Leslie Williams, Marcel Maier, Robert Dunstan, Stephen Salloway, Tianle Chen, Yan Ling, John O'Gorman, Fang Qian, Mahin Arastu, Mingwei Li, Sowmya Chollate, MelanieS Brennan, Omar Quintero-Monzon, RobertH Scannevin, HMoore Arnold, Thomas Engber, Kenneth Rhodes, James Ferrero, Yaming Hang, Alvydas Mikulskis, Jan Grimm, Christoph Hock, RogerM Nitsch, Alfred Sandrock
Nature 2016-09-01
Alzheimer's disease (AD) is characterized by deposition of amyloid-_ (A_) plaques and neurofibrillary tangles in the brain, accompanied by synaptic dysfunction and neurodegeneration. Antibody-based immunotherapy against A_ to trigger its clearance or mitigate its neurotoxicity has so far been unsuccessful. Here we report the gen...Read More

Product Numbers

Anti-Human IgG Peroxidase /catalog/product/sigma/a8667

TMB

PBS

Stop Reagent for TMB Substrate

NaCl

GuHCl

Sodium Carbonate

Hot Papers

Here are 5 of the most trending papers on Sigma-Aldrich.com in August. These hot papers have helped our customers further their research.  Sigma-Aldrich provides both a wide array of life-science and chemical products along with a wide-array of peer-reviewed papers.

Ethan B Secor, Mark C Hersam
The Journal of Physical Chemistry Letters 2015-02-19
Carbon and post-carbon nanomaterials present desirable electrical, optical, chemical, and mechanical attributes for printed electronics, offering low-cost, large-area functionality on flexible substrates. In this Perspective, recent developments in carbon nanomaterial inks are highlighted. Monodisperse semiconducting single-wall...Read More
Nan Zheng, Can Zhao, Xi-Ran He, Shan-Tong Jiang, Shu-Yan Han, Guo-Bing Xu, Ping-Ping Li
Journal of Chromatography B 2016-02-01
Gefitinib (Iressa) is the first oral EGFR tyrosine kinase inhibitor and it brings benefits to non-small cell lung cancer patients with EGFR mutation. In this study, a simple, rapid and credible high performance liquid chromatography-tandem mass spectrometry method was established and validated for the simultaneous quantification...Read More
C E Kung, J K Reed
Biochemistry (Washington) 1989-08-08
9-(Dicyanovinyl)julolidine (DCVJ) is a fluorescent dye whose intramolecular rotational relaxation is solvent dependent. Since its quantum yield increases with decreasing free volume, this molecule has been very useful in monitoring synthetic polymer reactions and measuring local microviscosity changes in phospholipid bilayers [L...Read More
Yanhong Shi, Ling-Zhi Xu, Kangsheng Peng, Wei Wu, Ruijin Wu, Zhi-Qiang Liu, Gui Yang, Xiao-Rui Geng, Jun Liu, Zhi-Gang Liu, Zhanju Liu, Ping-Chang Yang
Scientific reports 2015-01-01
The current therapy on allergic inflammation is unsatisfactory. Probiotics improve the immunity in the body. This study aims to test a hypothesis that administration with Clostridium butyricum (C. butyricum) enforces the effect of specific immunotherapy (SIT) on intestinal allergic inflammation. In this study, an ovalbumin (OVA)...Read More
Salma N Tammam, Hassan M E Azzazy, Hans G Breitinger, Alf Lamprecht
Molecular Pharmaceutics 2015-12-07
Many recently discovered therapeutic proteins exert their main function in the nucleus, thus requiring both efficient uptake and correct intracellular targeting. Chitosan nanoparticles (NPs) have attracted interest as protein delivery vehicles due to their biocompatibility and ability to escape the endosomes offering high potent...Read More

August 2016

Metabolite in Pomegranates found to extend lifespans

A recent published study examined the effects of Urolithin A, a metabolite found in Pomegranates. The study found that Urolithin A extended different animal model’s life spans, specifically in C. elegans. However the study also showed improved muscle function in rodents. Although not yet tested in humans, this might lead towards dietary changes or supplements that could increase human lifespans or quality of life.


Nature Medicine 2016-08-01
The biological effects of urolithins remain poorly characterized, despite wide-spread human exposure via the dietary consumption of their metabolic precursors, the ellagitannins, which are found in the pomegranate fruit, as well as in nuts and berries. We identified urolithin A (UA) as a first-in-class natural compound that indu...Read More

Hot Papers

Here are 5 of the most trending papers on Sigma-Aldrich.com in July. These hot papers have helped our customers further their research. Sigma-Aldrich provides both a wide array of life-science and chemical products along with a wide-array of peer-reviewed papers.

Su-Yeon You, Sea-Gwan Oh, Hye Min Han, Wujin Jun, Young-Shick Hong, Hyun-Jung Chung
International Journal of Biological Macromolecules 2016-01-01
The in vitro digestibility as well as the molecular and crystalline structures of waxy rice starches isolated from brown rice, germinated brown rice (GBR), ultrasonicated GBR, and heat-moisture treated GBR were investigated. The germinated brown rice starch (GBRS) had a lower average molecular weight and a higher proportion of D...Read More
Jianfei Xue, Aidong Zhou, Christina Tan, Yamei Wu, Hsueh-Te Lee, Wenliang Li, Keping Xie, Suyun Huang
Journal of Biological Chemistry 2015-07-24
The transcription factors glioma-associated oncogene homolog 1 (GLI1), a primary marker of Hedgehog pathway activation, and Forkhead box M1 (FOXM1) are aberrantly activated in a wide range of malignancies, including glioma. However, the mechanism of nuclear localization of GLI1 and whether FOXM1 regulates the Hedgehog signaling ...Read More
Zahra Kadri, Carine Lefevre, Olivier Goupille, Tipparat Penglong, Marine Granger-Locatelli, Suthat Fucharoen, Leila Maouche-Chretien, Philippe Leboulch, Stany Chretien
Genes & Development 2015-12-15
Tight coordination of cell proliferation and differentiation is central to red blood cell formation. Erythropoietin controls the proliferation and survival of red blood cell precursors, while variations in GATA-1/FOG-1 complex composition and concentrations drive their maturation. However, clear evidence of cross-talk between mo...Read More
Yen Ta Huang, Yi Ya Chen, Yu Hsien Lai, Chuan Chu Cheng, Tzu Chun Lin, Ying Shih Su, Chin Hung Liu, Pei Chun Lai
International Journal of Molecular Medicine 2016-01-01
Radiocontrast-induced nephropathy (RIN) is one of the leading causes of hospital-acquired acute kidney injury (AKI). The clinical strategies currently available for the prevention of RIN are insufficient. In this study, we aimed to determine whether resveratrol, a polyphenol phytoalexin, can be used to prevent RIN. For this purp...Read More
Lili Zhou, Hongyan Mo, Jinhua Miao, Dong Zhou, Roderick J Tan, Fan Fan Hou, Youhua Liu
The American Journal of Pathology 2015-12-01
Loss of Klotho and activation of the renin-angiotensin system (RAS) are common pathological findings in chronic kidney diseases. However, whether these two events are intricately connected is poorly understood. We hypothesized that Klotho might protect kidneys by targeted inhibition of RAS activation in diseased kidneys. To test...Read More

July 2016

Bioink developed to help further 3D Bioprinting

3D printing, already useful for industrial purposes, now is being used to rapidly advance tissue engineering. Bioink development is heavily studied for the purposes of creating better engineered tissue constructs. In this paper, a novel bioink utilizes sodium alginate and a pluronic detergent. This bioink combined with a new printing methodology helped improve the layering ability of the ink along with improved structural integrity. As 3D printed tissue engineering continues to improve, personally engineered tissue replacement may become a standard treatment.

James PK Armstrong, Madeline Burke, Benjamin M Carter, Sean A Davis, Adam W Perriman
Advanced Healthcare Materials 2016-06-01
3D tissue printing with adult stem cells is reported. A novel cell-containing multicomponent bioink is used in a two-step 3D printing process to engineer bone and cartilage architectures.Read More

Hot Papers on Sigma-Aldrich.com

Here are 5 of the most trending papers on Sigma-Aldrich.com in June. These hot papers have helped our customers further their research. Sigma-Aldrich provides both a wide array of life-science and chemical products along with a wide-array of peer-reviewed papers.

Iu V Karpets, Iu E Kolupaev, T O Iastreb, A I Oboznyĭ, N V Shvidenko, A A Lugovaia, A A Vaĭner
2013-01-01
The participation of reactive oxygen species (ROS) and calcium ions in realization of influence of exogenous jasmonic acid (JA) on the heat resistance of wheat coleoptiles has been investigated. Influence of 1 microM JA caused the transitional intensifying of generation of superoxide anion-radical (O2*-) and hydrogen peroxide in...Read More
Young Mi Song, Yong-ho Lee, Ji-Won Kim, Dong-Sik Ham, Eun-Seok Kang, Bong Soo Cha, Hyun Chul Lee, Byung-Wan Lee
Autophagy 2015-01-01
Metformin activates both PRKA and SIRT1. Furthermore, autophagy is induced by either the PRKA-MTOR-ULK1 or SIRT1-FOXO signaling pathways. We aimed to elucidate the mechanism by which metformin alleviates hepatosteatosis by examining the molecular interplay between SIRT1, PRKA, and autophagy. ob/ob mice were divided into 3 groups...Read More
Zhichao Liu, Qiang Shi, Don Ding, Reagan Kelly, Hong Fang, Weida Tong
PLoS computational biology 2011-12-01
Drug-induced liver injury (DILI) is a significant concern in drug development due to the poor concordance between preclinical and clinical findings of liver toxicity. We hypothesized that the DILI types (hepatotoxic side effects) seen in the clinic can be translated into the development of predictive in silico models for use in ...Read More
Megumi Tasaka-Fujita, Nao Sugiyama, Wonseok Kang, Takahiro Masaki, Takahiro Masaski, Asako Murayama, Norie Yamada, Ryuichi Sugiyama, Senko Tsukuda, Koichi Watashi, Yasuhiro Asahina, Naoya Sakamoto, Takaji Wakita, Eui-Cheol Shin, Takanobu Kato
Scientific reports 2015-01-01
Amino acid (aa) polymorphisms in the hepatitis C virus (HCV) genotype 1b core protein have been reported to be a potent predictor for poor response to interferon (IFN)-based therapy and a risk factor for hepatocarcinogenesis. We investigated the effects of these polymorphisms with genotype 1b/2a chimeric viruses that contained p...Read More
Yu-Tsai Yang, Anthony J Di Pasqua, Weiling He, Tsuimin Tsai, Katsuhiko Sueda, Yong Zhang, Michael Jay
Carbohydrate polymers 2013-02-15
A penta-ethyl ester prodrug of the radionuclide decorporation agent diethylenetriaminepentaacetic acid (DTPA), which exists as an oily liquid, was encapsulated in alginate beads by the ionotropic gelation method. An optimal formulation was found by varying initial concentrations of DTPA penta-ethyl ester, alginate polymer, Tween...Read More

June 2016

CRISPR used to eliminate HIV-1 in vivo

CRISPR is a versatile tool whose capabilities are still being explored. In a recent proof-of-concept study, CRISPR was used to eliminate HIV-1 genes in latent cells. A CRISPR was designed to target the U3 region of the HIV-1 long term repeat, which is used to create guide RNAs. Viral replication in the rat’s CD4+ T-cells were found to be eliminated. Although this is not yet a cure for HIV in humans, this points to a possible path to fully cure those whom are infected.

R Kaminski, R Bella, C Yin, J Otte, P Ferrante, HE Gendelman, H Li, R Booze, J Gordon, W Hu, K Khalili
Gene Therapy 2016-05-01
A CRISPR/Cas9 gene editing strategy has been remarkable in excising segments of integrated HIV-1 DNA sequences from the genome of latently infected human cell lines and by introducing InDel mutations, suppressing HIV-1 replication in patient-derived CD4+ T-cells, ex vivo. Here, we employed a short version of the Cas9 endonucleas...Read More

Hot Papers on Sigma-Aldrich.com

Here are 5 of the most trending papers on Sigma-Aldrich.com in May. These hot papers have helped our customers further their research. Sigma-Aldrich provides both a wide array of life-science and chemical products along with a wide-array of peer-reviewed papers.

Dalong Yu, Ping Wang, Yanjun Zhao, Aiping Fan
Talanta 2016-01-01
In this study, we found that iodophenol blue can enhance the weak chemiluminescence (CL) of luminol-H2O2 system. With the aid of CL spectral, electron spin resonance (ESR) spectral measurements and studies on the effects of various free radical scavengers on the iodophenol blue-enhanced luminol-H2O2 system, we speculated that io...Read More
Jialin Duan, Ying Yin, Guo Wei, Jia Cui, Enhu Zhang, Yue Guan, Jiajia Yan, Chao Guo, Yanrong Zhu, Fei Mu, Yan Weng, Yanhua Wang, Xiaoxiao Wu, Miaomiao Xi, Aidong Wen
Scientific reports 2015-01-01
Hyperglycemia-induced reactive oxygen species (ROS) generation and Ca(2+) overload contribute to the development of diabetic cardiomyopathy. In this study, we aimed to study the protective effects of Chikusetsu saponin IVa (CHS) from Aralia taibaiensis against hyperglycemia-induced myocardial injuries. Treatment of H9c2 cells wi...Read More
Sanjib K Shrestha, Marina Y Fosso, Sylvie Garneau-Tsodikova
Scientific reports 2015-01-01
Azoles are antifungal drugs used to treat fungal infections such as candidiasis in humans. Their extensive use has led to the emergence of drug resistance, complicating antifungal therapy for yeast infections in critically ill patients. Combination therapy has become popular in clinical practice as a potential strategy to fight ...Read More
Kevser Sözgen Başkan, Esma Tütem, Esin Akyüz, Seda Özen, Reşat Apak
Talanta 2016-01-15
As the concentration of reducing sugars (RS) is controlled by European legislation for certain specific food and beverages, a simple and sensitive spectrophotometric method for the determination of RS in various food products is proposed. The method is based on the reduction of Cu(II) to Cu(I) with reducing sugars in alkaline me...Read More
Hitoshi Murata, Hitoshi Takamatsu, Sulai Liu, Ken Kataoka, Nam-Ho Huh, Masakiyo Sakaguchi
PLoS ONE 2015-01-01
Mutations of the PTEN-induced putative kinase 1 (PINK1) gene are a cause of autosomal recessive forms of Parkinson's disease. Recent studies have revealed that PINK1 is an essential factor for controlling mitochondrial quality, and that it protects cells from oxidative stresses. Although there has been considerable progress in t...Read More

May 2016

IL-33 Protein Found to Remove Alzheimer Plaques

Alzheimer’s disease has been studied for years to help discover an effective way of eliminating it’s main cause, Beta-Amyloid plaques. A recent study in an alzheimer’s  mouse model has found that the protein IL-33 can reverse the effects of alzheimer's. It was observed that, after treating the mice with IL-33, harmful plaques were removed and brain plasticity increased. It is unknown how this will affect humans, but gives hope to an eventual cure.

AmyKY Fu, Kwok-Wang Hung, MichaelYF Yuen, Xiaopu Zhou, DeejaySY Mak, IvyCW Chan, TomH Cheung, Baorong Zhang, Wing-Yu Fu, FooY Liew, NancyY Ip
PNAS 2016-04-01
Alzheimer's disease (AD) is a devastating condition with no known effective treatment. AD is characterized by memory loss as well as impaired locomotor ability, reasoning, and judgment. Emerging evidence suggests that the innate immune response plays a major role in the pathogenesis of AD. In AD, the accumulation of β-amyloid (A...Read More

Hot Papers on Sigma-Aldrich.com

Here are 5 of the hottest and most popular scholarly articles on Sigma-Aldrich.com in April. Hot papers are scientific papers on Sigma-Aldrich.com that have become popular papers in the past month. These scholarly articles tend to be highly viewed papers for weeks after they first become popular papers.

Carina Sollert, Karthik Devaraj, Andreas Orthaber, Paul J Gates, Lukasz T Pilarski
Chemistry: A European Journal 2015-03-27
The Ru-catalysed C2-H arylation of indoles and pyrroles by using boronic acids under oxidative conditions is reported. This reaction can be applied to tryptophan derivatives and tolerates a wide range of functional groups on both coupling partners, including bromides and iodides, which can be further derivatised selectively. New...Read More
Charlène Pouech, Agneta Kiss, Florent Lafay, Didier Léonard, Laure Wiest, Cécile Cren-Olivé, Emmanuelle Vulliet
Journal of chromatography. A 2015-12-04
Polymer items are extensively present in the human environment. Humans may be consequently exposed to some compounds, such as additives, incorporated in these items. The objective of this work is to assess the human exposure to the main additives such as those authorized in the packaging for pharmaceutical products. The urinary ...Read More
Ewa Grela, Adam Ząbek, Agnieszka Grabowiecka
Avicenna Journal of Medical Biotechnology 2015-01-01
The chromogenic assay based on MTT bioreduction was adapted to Proteus mirabilis viability estimations. We primarily intended to use the assay for the evaluation of novel antimicrobial compounds, including structures with possible permeabilizing activity. Therefore, the influence of basic permeabilizing agents like Triton X-100 ...Read More
XingHui Shen, Na Zhang, ZhenDong Wang, GuangYu Bai, Zhong Zheng, YanLi Gu, YanShuang Wu, Hui Liu, DongJie Zhou, Lei Lei
Scientific reports 2015-01-01
Autophagy is an essential cellular mechanism that degrades cytoplasmic proteins and organelles to recycle their components. Moreover, autophagy is essential for preimplantation development in mammals. Here we show that autophagy is also important for reprogramming in somatic cell nuclear transfer (SCNT). Our data indicate that u...Read More
Hitoshi Murata, Hitoshi Takamatsu, Sulai Liu, Ken Kataoka, Nam-Ho Huh, Masakiyo Sakaguchi
PLoS ONE 2015-01-01
Mutations of the PTEN-induced putative kinase 1 (PINK1) gene are a cause of autosomal recessive forms of Parkinson's disease. Recent studies have revealed that PINK1 is an essential factor for controlling mitochondrial quality, and that it protects cells from oxidative stresses. Although there has been considerable progress in t...Read More

April 2016

CHRNA3/5 and APOE have significant effect on the human lifespan

Diseases such as lung cancer and diabetes have shown to shorten the lifespan of humans. What hasn't been shown is that regardless of disease state, specific markers can affect human lifespan as well. A recent study examining common gene variants of CHRNA3/5 and APOE have shown to significantly affect the lifespan of both men and women. Being heterozygous in APOE and CHRNA3/5 has shown a decrease life span of a couple years, while being homozygous has shown to decrease of up to 3.7 years.

Peter K Joshi, Krista Fischer, Katharina E Schraut, Harry Campbell, Tõnu Esko, James F Wilson
Nature Communications 2016-01-01
Lifespan is a trait of enormous personal interest. Research into the biological basis of human lifespan, however, is hampered by the long time to death. Using a novel approach of regressing (272,081) parental lifespans beyond age 40 years on participant genotype in a new large data set (UK Biobank), we here show that common vari...Read More

Hot Papers on Sigma-Aldrich.com

Here are 5 of the hottest and most popular scholarly articles on Sigma-Aldrich.com in March. Hot papers are scientific papers on Sigma-Aldrich.com that have become popular papers in the past month. These scholarly articles tend to be highly viewed papers for weeks after they first become popular papers.

E D Zarnowska, F C Rodgers, I Oh, V Rau, C Lor, K T Laha, R Jurd, U Rudolph, E I Eger, R A Pearce
Neuropharmacology 2015-06-01
Enhancement of tonic inhibition mediated by extrasynaptic α5-subunit containing GABAA receptors (GABAARs) has been proposed as the mechanism by which a variety of anesthetics, including the general anesthetic etomidate, impair learning and memory. Since α5 subunits preferentially partner with β3 subunits, we tested the hypothesi...Read More
Brijeshkumar S Patel, Wai Sie Co, Claudia Donat, Mary Wang, Wenchi Che, Pavan Prabhala, Friederike Schuster, Vera Schulz, Janet L Martin, Alaina J Ammit
Cancer Biology & Therapy 2015-01-01
Mitogen activated protein kinase phosphatase-1 (MKP-1) has emerged as an important protein mediating breast cancer oncogenesis and chemoresistance to cancer chemotherapies, especially proteasome inhibitors. In this in vitro study, we utilized the breast cancer epithelial cell lines MCF-7 and MDA-MB-231, in comparison to MCF-10A ...Read More
Ying Li, Jia Li, Shanshan Li, Yi Li, Xiangxiang Wang, Baolin Liu, Qiang Fu, Shiping Ma
Toxicology and Applied Pharmacology 2015-07-01
Curcumin is a natural polyphenolic compound in Curcuma longa with beneficial effects on neuronal protection. This study aims to investigate the action of curcumin in the hippocampus subjected to glutamate neurotoxicity. Glutamate stimulation induced reactive oxygen species (ROS), endoplasmic reticulum stress (ER stress) and TXNI...Read More
Bilal Rah, Reyaz ur Rasool, Debasis Nayak, Syed Khalid Yousuf, Debaraj Mukherjee, Lekha Dinesh Kumar, Anindya Goswami
Autophagy 2015-01-01
An active medicinal component of plant origin with an ability to overcome autophagy by inducing apoptosis should be considered a therapeutically active lead pharmacophore to control malignancies. In this report, we studied the effect of concentration-dependent 3-AWA (3-azido withaferin A) sensitization to androgen-independent pr...Read More
Tobias M Bingold, Katharina Franck, Katharina Holzer, Kai Zacharowski, Wolf O Bechstein, Heimo Wissing, Bertram Scheller
Surgical Infections 2015-06-01
Intestinal fatty acid binding protein (iFABP) is elevated in plasma by intestinal injury. We investigated the influence of surgical trauma and severe sepsis caused by abdominal and pulmonary infection on plasma iFABP concentrations. Seventy-nine patients were included in this prospective observational study: 31 patients before e...Read More

March 2016

Link between Iron Supplements and DNA Damage Response Discovered

A recently published scholarly article has shown that low dose Iron can induce DNA damage.  Six hours after iron exposure, a greater expression of DNA repair genes FANCG, BLM, and H2AFX were observed.  Although the study was done in cultured endothelial cells, this article shows that supplements should be studied further to see if any health effects are observed.

Inês G Mollet, Dilipkumar Patel, Fatima S Govani, Adam Giess, Koralia Paschalaki, Manikandan Periyasamy, Elaine C Lidington, Justin C Mason, Michael D Jones, Laurence Game, Simak Ali, Claire L Shovlin
PLoS ONE 2016-01-01
Spontaneous reports from patients able to report vascular sequelae in real time, and recognition that serum non transferrin bound iron may reach or exceed 10μmol/L in the blood stream after iron tablets or infusions, led us to hypothesize that conventional iron treatments may provoke acute vascular injury. This prompted us to ex...Read More

Hot Papers on Sigma-Aldrich.com

Here are 5 of the hottest and most popular scholarly articles on Sigma-Aldrich.com in February. Hot papers are scientific papers on Sigma-Aldrich.com that have become popular papers in the past month. These scholarly articles tend to be highly viewed papers for weeks after they first become popular papers.

Mehri Haeili, Casey Moore, Christopher J C Davis, James B Cochran, Santosh Shah, Tej B Shrestha, Yaofang Zhang, Stefan H Bossmann, William H Benjamin, Olaf Kutsch, Frank Wolschendorf
Antimicrobial Agents and Chemotherapy 2014-07-01
Macrophages take advantage of the antibacterial properties of copper ions in the killing of bacterial intruders. However, despite the importance of copper for innate immune functions, coordinated efforts to exploit copper ions for therapeutic interventions against bacterial infections are not yet in place. Here we report a novel...Read More
Jiao Ma, Pin Lu, Ailin Guo, Shuhua Cheng, Hongliang Zong, Peter Martin, Morton Coleman, Y Lynn Wang
British Journal of Haematology 2014-09-01
Ibrutinib inhibits Bruton tyrosine kinase (BTK), a key component of early B-cell receptor (BCR) signalling pathways. A multicentre phase 2 trial of ibrutinib in patients with relapsed/refractory mantle cell lymphoma (MCL) demonstrated a remarkable response rate. However, approximately one-third of patients have primary resistanc...Read More
Michael Weber, Christine Hellriegel, Alexander Rueck, Juerg Wuethrich, Peter Jenks, Markus Obkircher
Analytical and Bioanalytical Chemistry 2015-04-01
Quantitative nuclear magnetic resonance (qNMR) spectroscopy is employed by an increasing number of analytical and industrial laboratories for the assignment of content and quantitative determination of impurities. Within the last few years, it was demonstrated that (1)H qNMR can be performed with high accuracy leading to measure...Read More
Michael E Kurczy, Zheng-Jiang Zhu, Julijana Ivanisevic, Adam M Schuyler, Kush Lalwani, Antonio F Santidrian, John M David, Anand Giddabasappa, Amanda J Roberts, Hernando J Olivos, Peter J O'Brien, Lauren Franco, Matthew W Fields, Liliana P Paris, Martin Friedlander, Caroline H Johnson, Adrian A Epstein, Howard E Gendelman, Malcolm R Wood, Brunhilde H Felding, Gary J Patti, Mary E Spilker, Gary Siuzdak
Nature Communications 2015-01-01
Fluorocarbons are lipophobic and non-polar molecules that exhibit remarkable biocompatibility, with applications in liquid ventilation and synthetic blood. The unique properties of these compounds have also enabled mass spectrometry imaging of tissues where the fluorocarbons act as a Teflon-like coating for nanostructured surfac...Read More
Kun Liu, Enpeng Zhao, Ghulam Ilyas, Gadi Lalazar, Yu Lin, Muhammad Haseeb, Kathryn E Tanaka, Mark J Czaja
Autophagy 2015-01-01
Recent evidence that excessive lipid accumulation can decrease cellular levels of autophagy and that autophagy regulates immune responsiveness suggested that impaired macrophage autophagy may promote the increased innate immune activation that underlies obesity. Primary bone marrow-derived macrophages (BMDM) and peritoneal macro...Read More

February 2016

Genetic connection between Colorectal Cancer and Obesity discovered

Obesity has been known to influence colorectal cancer but the precise genetic link was not known. A recent scholarly article has shown that diet-induced obesity caused a loss of guanylin expression in the colon. This expression loss resulted in GUCY2C silencing and subsequently the creation of tumors. These chain of events clearly show the genetic connection between obesity and colorectal cancer in this scholarly article.

Jieru E Lin, Francheska Colon-Gonzalez, Erik Blomain, Gilbert W Kim, Amanda Aing, Brian Stoecker, Justin Rock, Adam E Snook, Tingting Zhan, Terry M Hyslop, Michal Tomczak, Richard S Blumberg, Scott A Waldman
Cancer Research 2016-01-01
Obesity is a well-known risk factor for colorectal cancer but precisely how it influences risks of malignancy remains unclear. During colon cancer development in humans or animals, attenuation of the colonic cell surface receptor guanylyl cyclase C (GUCY2C) that occurs due to loss of its paracrine hormone ligand guanylin contrib...Read More

Hot Papers on Sigma-Aldrich.com

Here are 5 of the most trending papers on Sigma-Aldrich.com in January. These hot papers have helped our customers further their research. Sigma-Aldrich provides both a wide array of life-science and chemical products along with a wide-array of peer-reviewed papers.

Hyun Lee, Jong Kil Lee, Min Hee Park, Yu Ri Hong, Hugo H Marti, Hyongbum Kim, Yohei Okada, Makoto Otsu, Eul-Ju Seo, Jae-Hyung Park, Jae-Hoon Bae, Nozomu Okino, Xingxuan He, Edward H Schuchman, Jae-Sung Bae, Hee Kyung Jin
Nature Communications 2014-01-01
Sphingosine is a major storage compound in Niemann-Pick type C disease (NP-C), although the pathological role(s) of this accumulation have not been fully characterized. Here we found that sphingosine kinase (SphK) activity is reduced in NP-C patient fibroblasts and NP-C mouse Purkinje neurons (PNs) due to defective vascular endo...Read More
Wei Wang, Michael Fromm
PLoS ONE 2015-01-01
Conjugated linoleic acid (CLA) reduces adiposity in human and mouse adipocytes. This outcome is achieved through a variety of biological responses including increased energy expenditure and fatty acid oxidation, increased inflammation, repression of fatty acid biosynthesis, attenuated glucose transport, and apoptosis. In the cur...Read More
Li Ding, Lingling Han, Yin Li, Jing Zhao, Ping He, Weizhen Zhang
Neoplasia 2014-11-01
The role of tuberous sclerosis complex (TSC) in the pathogenesis of pancreatic cancers remains largely unknown. The present study shows that neurogenin 3 directed Cre deletion of Tsc1 gene induces the development of pancreatic acinar carcinoma. By cross-breeding the Neurog3-cre mice with Tsc1loxp/loxp mice, we generated the Neur...Read More
Halina Abramczyk, Jakub Surmacki, Monika Kopeć, Alicja Klaudia Olejnik, Katarzyna Lubecka-Pietruszewska, Krystyna Fabianowska-Majewska
Analyst (Cambridge UK) 2015-04-07
We have studied live non-malignant (MCF10A), mildly malignant (MCF7) and malignant (MDA-MB-231) breast cancer cells and human breast cancer tissue. We demonstrate the first application of Raman imaging and spectroscopy in diagnosing the role of lipid droplets in cell line cultures that closely mimic an in vivo environment of var...Read More
Benjamin R Mercado, Kenneth J Nieser, Peter J Collings
Journal of Physical Chemistry B 2014-11-20
IR-806 is a near-infrared cyanine dye that undergoes a two-step assembly process in aqueous solutions. The final assemblies orientationally order into a liquid crystal at a very low concentration (∼0.6 wt % at room temperature). While the first step of the assembly process is continuous as the dye concentration or temperature is...Read More

January 2016

EPPS molecule found to remove Alzheimer causing Beta-amyloid plaques

One possible route to a cure for Alzheimers is to find a simple way to eliminate Beta-amyloid plaques. A recent study has shown that the EPPS molecule will bind to Beta-amyloid aggregates (plaques) and convert them into benign monomers. Although this was done in a mouse model, it is hopeful that the results in this and other scientific papers could also shed light onto a cure for Alzheimer’s in humans.

Hye Yun Kim, Hyunjin Vincent Kim, Seonmi Jo, C Justin Lee, Seon Young Choi, Dong Jin Kim, YoungSoo Kim
Nature Communications 2015-01-01
Alzheimer's disease (AD) is characterized by the transition of amyloid-β (Aβ) monomers into toxic oligomers and plaques. Given that Aβ abnormality typically precedes the development of clinical symptoms, an agent capable of disaggregating existing Aβ aggregates may be advantageous. Here we report that a small molecule, 4-(2-hydr...Read More

Hot Papers on Sigma-Aldrich.com

Here are 5 of the most trending papers on Sigma-Aldrich.com in December. These hot papers have helped our customers further their research. Sigma-Aldrich provides both a wide array of life-science and chemical products along with a wide-array of peer-reviewed papers.

Zhi-Kuang Tan, Reza Saberi Moghaddam, May Ling Lai, Pablo Docampo, Ruben Higler, Felix Deschler, Michael Price, Aditya Sadhanala, Luis M Pazos, Dan Credgington, Fabian Hanusch, Thomas Bein, Henry J Snaith, Richard H Friend
Nature Nanotechnology 2014-09-01
Solid-state light-emitting devices based on direct-bandgap semiconductors have, over the past two decades, been utilized as energy-efficient sources of lighting. However, fabrication of these devices typically relies on expensive high-temperature and high-vacuum processes, rendering them uneconomical for use in large-area displa...Read More
Jing Peng, Hua-Nian Zhang, Zhi-Sheng Liu, Hua Xu, Yang Wang
International Journal of Clinical Pharmacology and Therapeutics 2014-08-01
This study was to establish the population pharmacokinetic (PPK) model of pharmacologically active metabolite of oxcarbazepine (OXC) and to estimate PPK parameters for the optimal individuation administration of OXC in Chinese children with epilepsy. The pharmacologically active metabolite, 10-monohydroxy derivative of OXC (MHD)...Read More
Che-Fan Huang, Yu-Hsuan Liu, Hwan-Ching Tai
Journal of Peptide Science 2015-02-01
Protein oxidation by reactive oxygen species has been associated with aging and neurodegenerative disorders, and histidine is one of the major oxidation targets due to its metal-chelating property and susceptibility to metal-catalyzed oxidation. 2-Oxohistidine, the major product of histidine oxidation, has been recently identifi...Read More
Luca Sanguigno, Massimiliano Minale, Ernesto Vannini, Guido Arato, Rodolfo Riccio, Agostino Casapullo, Maria Chiara Monti, Raffaele Riccio, Silvestro Formisano, Gianfranco Di Renzo, Ornella Cuomo
Journal of Ethnopharmacology 2015-01-15
Triticum vulgare has been extensively used in traditional medicine thanks to its properties of accelerating tissue repair. The aqueous extract of Triticum vulgare (TVE) is currently an active component used by Farmaceutici Damor in the manufacture of certain pharmaceutical products already marketed in Italy and abroad under the ...Read More
M Keshavarz, B Kaffashi
Pharmaceutical Development and Technology 2014-12-01
The rheological and drug release behavior of biopolymer nanocomposite gels based on the cellulose derivatives, formulated as the bioadhesive drug delivery platforms, were investigated. The bioadhesive gel is composed of the microcrystalline cellulose, sodium carboxymethyl cellulose and phosphate buffered saline (pH = 7.4 at 20 °...Read More

December 2015

Aspirin targets gene associated with Alzheimer's

Aspirin is one of the earliest and still very popular anti-inflammatory drugs.  A recent study has found that it also affects an important housekeeping gene GAPDH.  GAPDH has been found to have a key role in neurodegenerative diseases such as Alzheimer's and Parkinson's. Furthermore in this paper it is shown that derivatives of Aspirin, such as amorfrutins, have an even stronger binding to GAPDH.  This study helps to show that Aspirin, or one of its derivatives, could show the path to a cure to many neurodegenerative diseases.

Hyong Woo Choi, Miaoying Tian, Murli Manohar, Maged M Harraz, Sang-Wook Park, Frank C Schroeder, Solomon H Snyder, Daniel F Klessig
PLoS ONE 2015-01-01
The plant hormone salicylic acid (SA) controls several physiological processes and is a key regulator of multiple levels of plant immunity. To decipher the mechanisms through which SA's multiple physiological effects are mediated, particularly in immunity, two high-throughput screens were developed to identify SA-binding protein...Read More

Hot Papers on Sigma-Aldrich.com

Here are 5 of the most trending papers on Sigma-Aldrich.com in November. These hot papers have helped our customers further their research. Sigma-Aldrich provides both a wide array of life-science and chemical products along with a wide-array of peer-reviewed papers.

Eun Ji Chung, Laurie B Mlinar, Kathryn Nord, Matthew J Sugimoto, Emily Wonder, Francis J Alenghat, Yun Fang, Matthew Tirrell
Advanced Healthcare Materials 2015-02-18
Atherosclerosis is a multifactorial inflammatory disease that can progress silently for decades and result in myocardial infarction, stroke, and death. Diagnostic imaging technologies have made great strides to define the degree of atherosclerotic plaque burden through the severity of arterial stenosis. However, current technolo...Read More
Michele Modugno, Patrizia Banfi, Fabio Gasparri, Robert Borzilleri, Percy Carter, Lyndon Cornelius, Marco Gottardis, Ving Lee, Claudio Mapelli, Joseph G Naglich, Andrew Tebben, Gregory Vite, Wilma Pastori, Clara Albanese, Emiliana Corti, Dario Ballinari, Arturo Galvani
Experimental Cell Research 2015-03-15
Cancer cell survival is frequently dependent on the elevated levels of members of the Bcl-2 family of prosurvival proteins that bind to and inactivate BH3-domain pro-apoptotic cellular proteins. Small molecules that inhibit the protein-protein interactions between prosurvival and proapoptotic Bcl-2 family members (so-called "BH3...Read More
Hannah L Woo, Nicholas R Ballor, Terry C Hazen, Julian L Fortney, Blake Simmons, Karen Walston Davenport, Lynne Goodwin, Natalia Ivanova, Nikos C Kyrpides, Konstantinos Mavromatis, Tanja Woyke, Janet Jansson, Jeff Kimbrel, Kristen M DeAngelis
Standards in Genomic Sciences 2014-01-01
In an effort to discover anaerobic bacteria capable of lignin degradation, we isolated Klebsiella sp. strain BRL6-2 on minimal media with alkali lignin as the sole carbon source. This organism was isolated anaerobically from tropical forest soils collected from the Bisley watershed at the Ridge site in the El Yunque National For...Read More
K E Wood, E Mantzioris, R A Gibson, C E Ramsden, B S Muhlhausler
Prostaglandins, Leukotrienes and Essential Fatty Acids (PLEFA) 2015-04-01
This paper presents a systematic review of human studies investigating the effect of altering dietary omega-3 polyunsaturated fatty acid (n-3 PUFA) alpha-linolenic acid (ALA) and omega-6 polyunsaturated fatty acid (n-6 PUFA) linoleic acid (LA) intakes on n-3 long-chain polyunsaturated fatty acid (LCPUFA) status in adult humans. ...Read More
Paulina Carmona-Mora, Jocelyn Widagdo, Florence Tomasetig, Cesar P Canales, Yeojoon Cha, Wei Lee, Abdullah Alshawaf, Mirella Dottori, Renee M Whan, Edna C Hardeman, Stephen J Palmer
Human Genetics 2015-10-01
GTF2IRD1 is one of the three members of the GTF2I gene family, clustered on chromosome 7 within a 1.8 Mb region that is prone to duplications and deletions in humans. Hemizygous deletions cause Williams-Beuren syndrome (WBS) and duplications cause WBS duplication syndrome. These copy number variations disturb a variety of develo...Read More

November 2015

New SNP that influences BCAR1 gene found to decrease heart disease in women

Recently the SNP (single nucleotide polymorphism) rs4888378 has been found to decrease rates of cardiovascular disease (CVD) in women. That specific SNP, when it had adenosine on both strands, was found to cause a 20% slower rate of thickening of the carotid arteries. Even those who have Adenosine on one allele still had a 10% slower rate of artery thickening. This genetic difference expands the knowledge of genetic risk factors that cause cardiovascular disease and will help further studies to improve the health of those with the disease. 

Freya Boardman-Pretty, Andrew J P Smith, Jackie Cooper, Jutta Palmen, Lasse Folkersen, Anders Hamsten, Alberico L Catapano, Olle Melander, Jacqueline F Price, Meena Kumari, John E Deanfield, Mika Kivimäki, Karl Gertow, Andrea Baragetti, Giuseppe Danilo Norata, Steve E Humphries
Circulation: Cardiovascular Genetics 2015-10-01
Carotid intima-media thickness (IMT) is a marker of subclinical atherosclerosis that can predict cardiovascular disease events over traditional risk factors. This study examined the BCAR1-CFDP1-TMEM170A locus on chromosome 16, associated with carotid IMT and coronary artery disease in the IMT and IMT-Progression as Predictors of...Read More

Hot Papers on Sigma-Aldrich.com

Here are 5 of the most trending papers on Sigma-Aldrich.com in October. These hot papers have helped our customers further their research. Sigma-Aldrich provides both a wide array of life-science and chemical products along with a wide-array of peer-reviewed papers.

Harkewal Singh, Benjamin W Arentson, Donald F Becker, John J Tanner
PNAS 2014-03-04
Proline utilization A (PutA) proteins are bifunctional peripheral membrane flavoenzymes that catalyze the oxidation of L-proline to L-glutamate by the sequential activities of proline dehydrogenase and aldehyde dehydrogenase domains. Located at the inner membrane of Gram-negative bacteria, PutAs play a major role in energy metab...Read More
Xing Lin, Shijun Zhang, Renbin Huang, Ling Wei, Chunhong Liang, Yongxing Chen, Shujuan Lv, Shuang Liang, Xiaoyan Wu, Quanfang Huang
Biological & Pharmaceutical Bulletin 2014-01-01
This study examined the effect of genistein from Hydrocotyle sibthorpioides on lipopolysaccharide (LPS)/D-galactosamine (D-GalN)-induced acute hepatic failure. Compared to the model control, genistein treatment significantly protected against LPS/D-GalN-induced liver injury, as evidenced by the decrease in serum alanine and aspa...Read More
Ixchelt Cuaranta-Monroy, Zoltan Simandi, Zsuzsanna Kolostyak, Quang-Minh Doan-Xuan, Szilard Poliska, Attila Horvath, Gergely Nagy, Zsolt Bacso, Laszlo Nagy
Stem Cell Research 2014-07-01
Adipocyte differentiation and function have become the major research targets due to the increasing interest in obesity and related metabolic conditions. Although, late stages of adipogenesis have been extensively studied, the early phases remain poorly understood. Here we present that supplementing ascorbic acid (AsA) to the ad...Read More
Philippe Haouzi, Bruno Chenuel, Takashi Sonobe
Clinical Toxicology 2015-01-01
Severe H2S poisoning leads to death by rapid respiratory and cardiac arrest, the latter can occur within seconds or minutes in severe forms of intoxication. To determine the time course and the nature of H2S-induced cardiac arrest and the effects of high-dose hydroxocobalamin administered after the end of sulfide exposure. NaHS ...Read More
Gopinath Kasetty, Praveen Papareddy, Martina Kalle, Victoria Rydengård, Matthias Mörgelin, Barbara Albiger, Martin Malmsten, Artur Schmidtchen
Antimicrobial Agents and Chemotherapy 2011-06-01
Peptides of the C-terminal region of human thrombin are released upon proteolysis and identified in human wounds. In this study, we wanted to investigate minimal determinants, as well as structural features, governing the antimicrobial and immunomodulating activity of this peptide region. Sequential amino acid deletions of the p...Read More

October 2015

Cpf1 enzyme found to be an improvement over Cas9 in CRISPR gene editing

Cpf1 has recently been discovered as an alternative to Cas9 in CRISPR gene editing systems. Cpf1 has a couple notable advantages over Cas9 in CRISPR editing. One advantage is that it creates sticky ends for easier DNA insertion compared to Cas9, which leaves blunt ends. Another advantage is that the Cpf1 enzyme is smaller which improves the ability to enter into a cell. Improvements to CRISPR editing will help make CRISPR an even more versatile tool in gene editing.

Bernd Zetsche, Jonathan S Gootenberg, Omar O Abudayyeh, Ian M Slaymaker, Kira S Makarova, Patrick Essletzbichler, Sara E Volz, Julia Joung, John van der Oost, Aviv Regev, Eugene V Koonin, Feng Zhang
Cell 2015-09-30
The microbial adaptive immune system CRISPR mediates defense against foreign genetic elements through two classes of RNA-guided nuclease effectors. Class 1 effectors utilize multi-protein complexes, whereas class 2 effectors rely on single-component effector proteins such as the well-characterized Cas9. Here, we report character...Read More

Hot Papers on Sigma-Aldrich.com

Here are 5 of the most trending papers on Sigma-Aldrich.com in September. These hot papers have helped our customers further their research. Sigma-Aldrich provides both a wide array of life-science and chemical products along with a wide-array of peer-reviewed papers.

Wan Aini Wan Ibrahim, Krishna Veni Veloo, Mohd. Marsin Sanagi, Wan Aini Wan Ibrahim, Krishna Veni Veloo, Mohd. Marsin Sanagi
Journal of Chromatography A 2012-03-16
A novel sol–gel hybrid methyltrimethoxysilane–tetraethoxysilane (MTMOS–TEOS) was produced and applied as sorbent for solid phase extraction (SPE). Five selected organophosphorus pesticides (OPPs) were employed as model compounds to evaluate the extraction performance of the synthesized sol–gel organic–inorganic hybrid MTMOS–TEOS...Read More
Ning Lin, Cécile Bruzzese, Alain Dufresne
ACS Applied Materials & Interfaces 2012-09-26
Crosslinked polysaccharide sponges have been prepared by freeze-drying of amorphous alginate-oxidized nanocellulose in the presence of a Ca(2+) ionic crosslinking agent. The new carboxyl groups on the surface of nanocellulose induced by the chemical oxidization provided the possibility of participating in the construction of an ...Read More
Martin Jinek, Krzysztof Chylinski, Ines Fonfara, Michael Hauer, Jennifer A Doudna, Emmanuelle Charpentier
Science 2012-08-17
Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) systems provide bacteria and archaea with adaptive immunity against viruses and plasmids by using CRISPR RNAs (crRNAs) to guide the silencing of invading nucleic acids. We show here that in a subset of these systems, the mature crRNA that ...Read More
Huihui Yao, Rebecca A Jockusch
Journal of Physical Chemistry A: Molecules, Spectroscopy, Kinetics, Environment and General Theory 2013-02-14
2',7'-Dichloro- and 2',7'-difluorofluoresceins are superior alternatives to underivatized fluorescein. Although several studies characterizing their condensed-phase photophysical properties have been reported, little is known about their intrinsic characteristics. Here, the gas-phase properties of three charge states of each flu...Read More
Sarah Kerrigan, Ashley Mott, Breanna Jatzlau, Francisco Ortiz, Laura Perrella, Sarah Martin, Kelsie Bryand
Journal of Forensic Sciences 2014-01-01
Designer psychostimulants are known by recreational drug users to produce a complex array of adrenergic and hallucinogenic effects. Many of these drugs are not targeted during routine toxicology testing and as a consequence, they are rarely reported. The purpose of this study was to develop a procedure for the detection of 15 ps...Read More

September 2015

Peptide Array Developed to Study Peptide Cell Penetrating Abilities

Peptides introduced within a cell have been known to affect cellular activity. The most difficult issue has always been on how to introduce hydrophilic peptides into a cell past the cell's membrane while still maintaining its unique properties. An array has recently been developed to study peptide attributes when paired with cell penetrating peptides. This will hopefully increase the discovery of novel peptide based cures down the road. 

Ryo Matsumoto, Mina Okochi, Kazunori Shimizu, Kei Kanie, Ryuji Kato, Hiroyuki Honda
Scientific reports 2015-01-01
Peptides, especially intracellular functional peptides that can play a particular role inside a cell, have attracted attention as promising materials to control cell fate. However, hydrophilic materials like peptides are difficult for cells to internalize. Therefore, the screening and design of intracellular functional peptides ...Read More

Hot Papers on Sigma-Aldrich.com

Here are 5 of the most trending papers on Sigma-Aldrich.com in August. These hot papers have helped our customers further their research. Sigma-Aldrich provides both a wide array of life-science and chemical products along with a wide-array of peer-reviewed papers.

Gilles Ferry, Natacha Moulharat, Jean-Philippe Pradère, Patrice Desos, Anne Try, Annie Genton, Adeline Giganti, Monique Beucher-Gaudin, Michel Lonchampt, Marc Bertrand, Jean-Sébastien Saulnier-Blache, Gordon C Tucker, Alex Cordi, Jean A Boutin
Journal of Pharmacology and Experimental Therapeutics 2008-12-01
Autotaxin catalyzes the transformation of lyso-phosphatidylcholine in lyso-phosphatidic acid (LPA). LPA is a phospholipid possessing a large panel of activity, in particular as a motility factor or as a growth signal, through its G-protein coupled seven transmembrane receptors. Indirect evidence strongly suggests that autotaxin ...Read More
Chris Hamman, Donald E. Schmidt, Mengling Wong, Michael Hayes, Chris Hamman, Donald E. Schmidt, Mengling Wong, Michael Hayes
Journal of Chromatography A 2011-01-01
Highlights ► NH 4OH does not degrade the silica support under SFC conditions. ► NH 4OH evaporates leaving no residual additive in a purified fraction. ► NH 4OH provides similar chromatography as compared to diethylamine. ► NH 4OH greatly enhances electrospray ionization as compared to diethylamine. ►...Read More
Allison Dilzer, Yeonhwa Park
Critical Reviews in Food Science and Nutrition 2012-01-01
Conjugated linoleic acid (CLA) has drawn significant attention in the last two decades for its variety of biologically beneficial effects. CLA reduces body fat, cardiovascular diseases and cancer, and modulates immune and inflammatory responses as well as improves bone mass. It has been suggested that the overall effects of CLA ...Read More
Helen W Kreuzer, Juske Horita, James J Moran, Bruce A Tomkins, Derek B Janszen, April Carman
Journal of Forensic Sciences 2012-01-01
Sodium and potassium cyanide are highly toxic, produced in large amounts by the chemical industry, and linked to numerous high-profile crimes. The U.S. Centers for Disease Control and Prevention has identified cyanide as one of the most probable agents to be used in a chemical terrorism event. We investigated whether stable C an...Read More
Junko Murai, Yiping Zhang, Joel Morris, Jiuping Ji, Shunichi Takeda, James H Doroshow, Yves Pommier
Journal of Pharmacology and Experimental Therapeutics 2014-06-01
We recently showed that poly(ADP-ribose) polymerase (PARP) inhibitors exert their cytotoxicity primarily by trapping PARP-DNA complexes in addition to their NAD(+)-competitive catalytic inhibitory mechanism. PARP trapping is drug-specific, with olaparib exhibiting a greater ability than veliparib, whereas both compounds are pote...Read More

August 2015

The mosquito immune response, not the malaria parasite, changes the mosquito feed behavior

Modifying a mosquito's behavior has become a new tool in the fight against malaria. When infected with the malaria parasite, the mosquito’s feeding behavior seems to change. A recent study has shown that it is actually the mosquito's immune response to the parasite, not the parasite itself that makes this behavioral change. This was shown by infecting a mosquito with heat-attenuated E. coli. Learning more about how to modify a mosquito's feeding behavior will help prevent infections in the future.

Lauren J Cator, Jose E Pietri, Courtney C Murdock, Johanna R Ohm, Edwin E Lewis, Andrew F Read, Shirley Luckhart, Matthew B Thomas
Scientific reports 2015-07-08

Hot Papers on Sigma-Aldrich.com

Here are 5 of the most trending papers on Sigma-Aldrich.com in July. These hot papers have helped our customers further their research. Sigma-Aldrich provides both a wide array of life-science and chemical products along with a wide-array of peer-reviewed papers.

Jing Wu, Di Tu, Li-Yun Yuan, Jin-e Yi, Yanan Tian
Toxicology Letters 2015-02-03
T-2 toxin is a secondary metabolite produced by Fusarium genus and is a common contaminant in food and feedstuffs of cereal origin. In porcine granulosa cells(GC), T-2 toxin has been shown to inhibit the steroidogenesis; however, the mechanism has not been well understood. Gonadotropin-stimulated steroidogenesis is regulated by ...Read More
Cunxi Wang, Luis A Burzio, Michael S Koch, Andre Silvanovich, Erin Bell
Regulatory Toxicology and Pharmacology 2015-03-01
• CSPB has a history of safe use. • CSPB does not share any sequence similarity to known allergens or toxins. • The CSPB protein is susceptible to digestive enzymes. ...Read More
Masatake Fujimura, Fusako Usuki
Toxicology and Applied Pharmacology 2015-02-01
Methylmercury (MeHg) is an environmental toxin which induces cell death specific for the nervous systems. Here we show that MeHg causes neuronal cell death through the suppression of the tropomyosin receptor kinase A (TrkA) pathway, and that compounds activating the TrkA pathway prevent MeHg-induced nerve damage in vitro and in ...Read More
S N Saha, A K Sen
Vaccine 1989-08-01
Studies were undertaken to develop a cheaper medium with indigenous sources of peptone and casein hydrolysate for continuous culture of BHK-21 (suspension) cells and production of foot-and-mouth disease (FMD) vaccine. Eleven batches of experimental media were prepared using different indigenous sources of casein hydrolysate and ...Read More
Yu-Cai He, Feng Liu, Dan-Ping Zhang, Shan Gao, Zhi-Qing Li, Zhi-Cheng Tao, Cui-Luan Ma
Applied Biochemistry and Biotechnology 2015-03-01
In this study, it was the first report that Bacillus sp. CCZU11-1 was used for the biotransformation of 1,3-propanediol cyclic sulfate (1,3-PDS) and its derivatives. The catalytic performance of Bacillus sp. sulfatase in the biotransformation of 1,3-PDS was significantly improved by biocatalyst permeabilization and immobilizatio...Read More

July 2015

Unique gene variant in Papua New Guinea population prevents prion disease

In the 1950s Kuru, a prion disease, was rapidly spreading throughout Papua New Guinea. Once the practice of cannibalism was abolished the spread of the disease was brought under control.  A new study in Nature found that those with a V127 variant on the M129 PNPP allele were found to be resistant to Kuru and variants of Creutzfeld-Jakob disease (CJD). This study will hopefully lay the path to the cure of Prion diseases.

Emmanuel A Asante, Michelle Smidak, Andrew Grimshaw, Richard Houghton, Andrew Tomlinson, Asif Jeelani, Tatiana Jakubcova, Shyma Hamdan, Angela Richard-Londt, Jacqueline M Linehan, Sebastian Brandner, Michael Alpers, Jerome Whitfield, Simon Mead, Jonathan D F Wadsworth, John Collinge
Nature 2015-06-10
Mammalian prions, transmissible agents causing lethal neurodegenerative diseases, are composed of assemblies of misfolded cellular prion protein (PrP). A novel PrP variant, G127V, was under positive evolutionary selection during the epidemic of kuru-an acquired prion disease epidemic of the Fore population in Papua New Guinea-an...Read More

Hot Papers on Sigma-Aldrich.com

Here are 5 of the most trending papers on Sigma-Aldrich.com in June. These hot papers have helped our customers further their research. Sigma-Aldrich provides both a wide array of life-science and chemical products along with a wide-array of peer-reviewed papers.

Krzysztof Kuciński, Piotr Pawluć, Bogdan Marciniec, Grzegorz Hreczycho
Chemistry: A European Journal 2015-03-23
The first use of a Lewis acid catalyst in the addition reaction of both aromatic and aliphatic thiols to unsaturated organosilicon compounds is reported. In catalytic tests, scandium(III) triflate demonstrates high catalytic activity in this process. Under mild conditions (25 °C, room temperature, 1-10 h) a number of thioether-f...Read More
Mark D A van Logtestijn, Elisa Domínguez-Hüttinger, Georgios N Stamatas, Reiko J Tanaka
PLoS ONE 2015-01-01
The stratum corneum (SC) provides a permeability barrier that limits the inflow and outflow of water. The permeability barrier is continuously and dynamically formed, maintained, and degraded along the depth, from the bottom to the top, of the SC. Naturally, its functioning and structure also change dynamically in a depth-depend...Read More
N R Lemoine, E S Mayall, T Jones, D Sheer, S McDermid, P Kendall-Taylor, D Wynford-Thomas
British Journal of Cancer 1989-12-01
Human primary thyroid follicular epithelial cells were transfected with a plasmid containing an origin-defective SV40 genome (SVori-) to produce several immortal cell lines. Two of the 10 cell lines analysed expressed specific features of thyroid epithelial function (iodide-trapping and thyroglobulin production). These two lines...Read More
Moustafa N Aboushelib, Donia Sleem
Journal of Adhesive Dentistry 2014-12-01
To evaluate the influence of the internal structure of lithium disilicate glass ceramics (LDC) on the microtensile bond strength to a resin adhesive using two surface treatments. Milling blocks of three types of LDC were sectioned (4 mm thick) using a precision cutting machine: IPS Empress 2 (conventional LDC), IPSe.max CAD (a r...Read More
Coilín Boland, Dianfan Li, Syed Tasadaque Ali Shah, Stefan Haberstock, Volker Dötsch, Frank Bernhard, Martin Caffrey
Cellular and Molecular Life Sciences 2014-12-01
Membrane proteins are key elements in cell physiology and drug targeting, but getting a high-resolution structure by crystallographic means is still enormously challenging. Novel strategies are in big demand to facilitate the structure determination process that will ultimately hasten the day when sequence information alone can ...Read More

June 2015

Seasonal gene expression shows variation in Human immunity

Changing gene expression during different seasons has been observed in the past but not specifically with cells dealing with immunity. An extensive study has just been performed on mRNA isolated from immune cells. A wide difference was discovered in that ~23% of the whole genome shows significant variation seasonally. Specifically ARNTL expression in peripheral blood cells changed levels from Summer to Winter months.

Xaquin Castro Dopico, et al.
Nature Communications 2015-01-01
Seasonal variations are rarely considered a contributing component to human tissue function or health, although many diseases and physiological process display annual periodicities. Here we find more than 4,000 protein-coding mRNAs in white blood cells and adipose tissue to have seasonal expression profiles, with inverted patter...Read More

Hot Papers on Sigma-Aldrich.com

Here are 5 of the most trending papers on Sigma-Aldrich.com in May. These hot papers have helped our customers further their research. Sigma-Aldrich provides both a wide array of life-science and chemical products along with a wide-array of peer-reviewed papers.

Jayakumar Jerobin, R S Sureshkumar, C H Anjali, Amitava Mukherjee, Natarajan Chandrasekaran
Carbohydrate polymers 2012-11-06
Azadirachtin a biological compound found in neem have medicinal and pesticidal properties. The present work reports on the encapsulation of neem oil nanoemulsion using sodium alginate (Na-Alg) by cross linking with glutaraldehyde. Starch and polyethylene glycol (PEG) were used as coating agents for smooth surface of beads. The S...Read More
Hui-Chuan Yu, Duen-Ren Hou, Chun-Yu Liu, Chen-Si Lin, Chung-Wai Shiau, Ann-Lii Cheng, Kuen-Feng Chen
PLoS ONE 2013-01-01
Previously, we reported that cancerous inhibitor of protein phosphatase 2A (CIP2A) mediates the apoptotic effect of bortezomib in hepatocellular carcinoma (HCC). Here, we report a proteasome-independent mechanism by which bortezomib induces autophagy in HCC. Our data indicate that bortezomib activated autophagy in a dose- and ti...Read More
Yu-Chan Zhang, Yang Yu, Cong-Ying Wang, Ze-Yuan Li, Qing Liu, Jie Xu, Jian-You Liao, Xiao-Jing Wang, Liang-Hu Qu, Fan Chen, Peiyong Xin, Cunyu Yan, Jinfang Chu, Hong-Qing Li, Yue-Qin Chen
Nature Biotechnology 2013-09-01
Increasing grain yields is a major focus of crop breeders around the world. Here we report that overexpression of the rice microRNA (miRNA) OsmiR397, which is naturally highly expressed in young panicles and grains, enlarges grain size and promotes panicle branching, leading to an increase in overall grain yield of up to 25% in ...Read More
Tiago Coelho de Assis Lage, Ricardo Marques Montanari, Sergio Antonio Fernandes, Caio Márcio de Oliveira Monteiro, Tatiane de Oliveira Souza Senra, Viviane Zeringota, Renata da Silva Matos, Erik Daemon
Experimental Parasitology 2015-01-01
Baccharis dracunculifolia DC (common name "alecrim-do-campo" in Brazil) is a plant with widespread distribution in South America that is the botanical origin of green propolis. The aim of this study was to evaluate the chemical composition and acaricidal activity of the essential oil of B. dracunculifolia and its constituents ne...Read More
Ghassan Zuhair Abdullah, Muthanna Fawzy Abdulkarim, Chitneni Mallikarjun, Elrashid Saleh Mahdi, Mahiran Basri, Munavvar Abdul Sattar, Azmin Mohd Noor
Pakistan journal of pharmaceutical sciences 2013-01-01
Micro-emulsions and sometimes nano-emulsions are well known candidates to deliver drugs locally. However, the poor rheological properties are marginally affecting their acceptance pharmaceutically. This work aimed to modify the poor flow properties of a nano-scaled emulsion comprising palm olein esters as the oil phase and ibupr...Read More

May 2015

Agonists to Calcium-sensing receptor may be lead to an Asthma cure

For decades, asthma symptoms have been successfully treated using inhaled steroids.  Drugs to prevent the cause of the symptoms have been less successful. A new approach has been found to introduce agonists to the Calcium--sensing receptor (CaSR) and help prevent the symptoms from even beginning. By treating the actual cause of Asthma, a pre-symptomatic cure might soon be possible.

Paper

Polina L Yarova, et al.
Science translational medicine 2015-04-22
Airway hyperresponsiveness and inflammation are fundamental hallmarks of allergic asthma that are accompanied by increases in certain polycations, such as eosinophil cationic protein. Levels of these cations in body fluids correlate with asthma severity. We show that polycations and elevated extracellular calcium activate the human recombinant and native calcium-sensing receptor (CaSR), leading to intracellular calcium mobilization, cyclic adenosine monophosphate breakdown, and p38 mitogen-activated protein kinase phosphorylation in airway smooth muscle (ASM) cells...Read More

Hot Papers on Sigma-Aldrich.com

Here are 5 of the most trending papers on Sigma-Aldrich.com in April. These hot papers have helped our customers further their research. Sigma-Aldrich provides both a wide array of life-science and chemical products along with a wide-array of peer-reviewed papers.

Nadia Touisni, Alfonso Maresca, Paul C McDonald, Yuanmei Lou, Andrea Scozzafava, Shoukat Dedhar, Jean-Yves Winum, Claudiu T Supuran
Journal of Medicinal Chemistry 2011-12-22
A series of 7-substituted coumarins incorporating various glycosyl moieties were synthesized and investigated for the inhibition of the zinc enzyme carbonic anhydrase (CA, EC 4.2.1.1). These coumarins were very weak or ineffective as inhibitors of the housekeeping, off target isoforms CA I and II, but some of them inhibited tumo...Read More
Changbin Ju, Yongsheng Wang, Dawei He, Qi Gao, Lei Gao, Ming Fu
Journal of Nanoscience and Nanotechnology 2011-11-01
A composite of polyaniline (PANI) and nanoencapsulated phase-change material (PCM) with polystyrene (PS) as the shell and n-octadecane as the core was synthesized using the ultrasonic technique assisted by in situ polymerization. The composite's morphology, structure, and thermal properties were characterized by transmission ele...Read More
O Shpilberg, C Jackisch
British Journal of Cancer 2013-09-17
Rituximab and trastuzumab were the first therapeutic monoclonal antibodies (mAbs) approved in oncology. Both antibodies are delivered by the intravenous (IV) route, but recently subcutaneous (SC) formulations have been developed. Subcutaneous administration of mAbs can offer substantial patient and resource benefits compared wit...Read More
Vivek R Yadav, Kaustuv Sahoo, Vibhudutta Awasthi
British Journal of Pharmacology 2013-12-01
4-[3,5-Bis(2-chlorobenzylidene)-4-oxo-piperidine-1-yl]-4-oxo-2-butenoic acid CLEFMA is a new anti-cancer molecule. Here, we investigated changes in apoptosis and inflammatory markers during CLEFMA-induced tumour suppression. Lung adenocarcinoma H441 and A549, and normal lung fibroblast CCL151 cell lines were used, along with a x...Read More
Denise Martinez Alanis, Daniel R Chang, Haruhiko Akiyama, Mark A Krasnow, Jichao Chen
Nature Communications 2014-01-01
The lung is a branched tubular network with two distinct compartments--the proximal conducting airways and the peripheral gas exchange region--separated by a discrete boundary termed the bronchoalveolar duct junction (BADJ). Here we image the developing mouse lung in three-dimensions (3D) and show that two nested developmental w...Read More

April 2015

Scanning ultrasound removes harmful plaques from Alzheimer’s mouse model

Beta-Amyloid plaques have been studied for years now as the symptomatic marker of Alzheimer’s disease. It is believed that either preventing the plaque formation or eliminating those that have already formed is the key to treating Alzheimer’s disease. Scanning ultrasound, a non-pharmacological treatment, has been used to successfully remove Beta-Amyloid plaques in mice. Although not yet tested in humans, this illuminates a possible alternative treatment for Alzheimer’s down the road.

Paper

Gerhard Leinenga, Jürgen Götz
Science Translational Medicine 2015-03-11
Amyloid-β (Aβ) peptide has been implicated in the pathogenesis of Alzheimer's disease (AD). We present a nonpharmacological approach for removing Aβ and restoring memory function in a mouse model of AD in which Aβ is deposited in the brain. We used repeated scanning ultrasound (SUS) treatments of the mouse brain to remove Aβ, wi...Read More

Hot Papers on Sigma-Aldrich.com

Here are 5 of the most trending papers on Sigma-Aldrich.com in March. These hot papers have helped our customers further their research. Sigma-Aldrich provides both a wide array of life-science and chemical products along with a wide-array of peer-reviewed papers.

Ruth S Cruz Cosme, Yasuhiro Yamamura, Qiyi Tang
Journal of Virology 2009-04-01
Human cytomegalovirus (HCMV), a member of the beta subgroup of the family Herpesviridae, causes serious health problems worldwide. HCMV gene expression in host cells is a well-defined sequential process: immediate-early (IE) gene expression, early-gene expression, DNA replication, and late-gene expression. The most abundant IE g...Read More
Nathalie Schlienger, Birgitte W Lund, Jan Pawlas, Fabrizio Badalassi, Fabio Bertozzi, Rasmus Lewinsky, Alma Fejzic, Mikkel B Thygesen, Ali Tabatabaei, Stefania Risso Bradley, Luis R Gardell, Fabrice Piu, Roger Olsson
Journal of Medicinal Chemistry 2009-11-26
Herein we describe the discovery of ACP-105 (1), a novel and potent nonsteroidal selective androgen receptor modulator (SARM) with partial agonist activity relative to the natural androgen testosterone. Compound 1 was developed from a series of compounds found in a HTS screen using the receptor selection and amplification techno...Read More
Yu-Tsai Yang, Hsiung-Fei Chien, Po-Han Chang, Yee-Chun Chen, Michael Jay, Tsuimin Tsai, Chin-Tin Chen
Lasers in Surgery and Medicine 2013-03-01
Antimicrobial photodynamic inactivation (PDI) is a promising therapeutic modality for the treatment of local infections. To increase the efficacy of PDI, chlorine e6 (Ce6) was encapsulated in cationic CTAB-liposomes composed of various ratios of dimyristoyl-sn-glycero-phosphatidylcholine (DMPC) and the cationic surfactant, cetyl...Read More
Wei Wei, Mei-Sze Chua, Susan Grepper, Samuel So
International Journal of Cancer 2010-05-15
Hepatocellular carcinoma (HCC) is the 5th most common cancer worldwide. It is intrinsically resistant toward standard chemotherapy, making it imperative to develop novel selective chemotherapeutic agents. The Wnt/beta-catenin pathway plays critical roles in development and oncogenesis, and is dysregulated in HCC. Our study aims ...Read More
Qilong Wang, Bin Liang, Najeeb A Shirwany, Ming-Hui Zou
PLoS ONE 2011-01-01
Autophagy is a cellular self-digestion process activated in response to stresses such as energy deprivation and oxidative stress. However, the mechanisms by which energy deprivation and oxidative stress trigger autophagy remain undefined. Here, we report that activation of AMP-activated protein kinase (AMPK) by mitochondria-deri...Read More

March 2015

Dark Roast Coffee found to decrease DNA strand breaks

The beneficial effects of coffee have been studied heavily in the last few decades. Coffee has been found to prevent numerous degenerative diseases and even some forms of cancer. There have been studies on how coffee can decrease the oxidative damage on white blood cells, but this is the first study showing it to reduce the damage done to strands of DNA. As the scientific body of evidence builds the numerous positive health effects of coffee may outweigh the negative.

Paper

T Bakuradze, R Lang, T Hofmann, G Eisenbrand, D Schipp, J Galan, E Richling
European Journal of Nutrition 2015-02-01
Coffee consumption has been reported to decrease oxidative damage in peripheral white blood cells (WBC). However, effects on the level of spontaneous DNA strand breaks, a well established marker of health risk, have not been specifically reported yet...Read More


Hot Papers on Sigma-Aldrich.com

Here are 5 of the most trending papers on Sigma-Aldrich.com in February. These hot papers have helped our customers further their research. Sigma-Aldrich provides both a wide array of life-science and chemical products along with a wide-array of peer-reviewed papers.

Hitomi Takemura, Harue Uchiyama, Takeshi Ohura, Hiroyuki Sakakibara, Ryoko Kuruto, Takashi Amagai, Kayoko Shimoi
The Journal of Steroid Biochemistry and Molecular Biology 2010-01-01
A 17beta-estradiol (E(2)) is hydrolyzed to 2-hydroxy-E(2) (2-OHE(2)) and 4-hydroxy-E(2) (4-OHE(2)) via cytochrome P450 (CYP) 1A1 and 1B1, respectively. In estrogen target tissues including the mammary gland, ovaries, and uterus, CYP1B1 is highly expressed, and 4-OHE(2) is predominantly formed in cancerous tissues. In this study,...Read More
Mohamed Khairy, Sherif A El-Safty, Mohamed Ismael
Chemical Communications 2012-11-14
Nanomagnet-selective supercaptors of heme-proteins (iron-porphyrin prosthetic group) based on mesoporous NiO and Fe(3)O(4) NPs were fabricated.Read More
M Löhr, A Hoffmeyer, J Kröger, M Freund, J Hain, A Holle, P Karle, W T Knöfel, S Liebe, P Müller, H Nizze, M Renner, R M Saller, T Wagner, K Hauenstein, W H Günzburg, B Salmons
The Lancet 2001-05-19
Pancreatic cancer can seldom be resected, and chemotherapy has only a limited effect on survival or tumour load. We did a phase I/II trial in 14 patients with pancreatic cancer to assess the safety of local activation of low-dose ifosfamide. We encapsulated genetically modified allogeneic cells, which expressed a cytochrome P450...Read More
Philippe C Gros, Abdelatif Doudouh, Christopher Woltermann
Organic & Biomolecular Chemistry 2006-12-07
The first regioselective C-4 lithiation of (S)-nicotine has been realized using TMSCH2Li as basic reagent in toluene. The reaction proceeded under mild conditions with a small excess of electrophile. The 4-chloro derivative was subsequently metallated at C-5 with the same basic reagent in THF at -78 degrees C. This methodology o...Read More

February 2015

A Novel Antibiotic has Just Been Discovered

The threat of antibiotic resistant microbes has been steadily increasing in the last decade. Unfortunately the number of new antibiotics have not been able to keep pace with the rapid spread of resistant microbes. A novel antibiotic named Teixobactin has just been isolated and its study published in Nature. This study demonstrates the unique way that Eleftheria terrae, a newly discovered microbe which makes Teixobactin, was isolated and what makes this antibiotic so unique.

Paper

Losee L Ling, Tanja Schneider, Aaron J Peoples, Amy L Spoering, Ina Engels, Brian P Conlon, Anna Mueller, Till F Schäberle, Dallas E Hughes, Slava Epstein, Michael Jones, Linos Lazarides, Victoria A Steadman, Douglas R Cohen, Cintia R Felix, K Ashley Fetterman, William P Millett, Anthony G Nitti, Ashley M Zullo, Chao Chen, Kim Lewis
Nature 2015-01-22
Antibiotic resistance is spreading faster than the introduction of new compounds into clinical practice, causing a public health crisis. Most antibiotics were produced by screening soil microorganisms, but this limited resource of cultivable bacteria was overmined by the 1960s. Synthetic approaches to produce antibiotics have be...Read More

Hot Papers on Sigma-Aldrich.com

Here are 5 of the most trending papers on Sigma-Aldrich.com in January. These hot papers have helped our customers further their research. Sigma-Aldrich provides both a wide array of life-science and chemical products along with a wide-array of peer-reviewed papers.

René J Buesa, Maxim V Peshkov
Annals of Diagnostic Pathology 2009-08-01
After the hazardous effects of xylene became indisputable in the 1970s, many potential substitutes became available, some with as many if not more hazards. This article discusses the inadequacy of 5 vegetable oils as substitutes, as well as the characteristics of 22 D-limonene-based substitutes, all less effective in their chemi...Read More
Md Moinuddin Ahmed, Matthew S Mortensen, George A O'Doherty
Journal of Organic Chemistry 2006-09-29
The enantioselective syntheses of several protected 4-substituted syn-3,5-dihydroxy carboxylic esters have been achieved from the corresponding achiral (E,E)- or (E,Z)-1,3-dienoates. The route relies upon an enantio- and regioselective Sharpless dihydroxylation and a palladium-catalyzed reduction to form gamma-substituted delta-...Read More
Thomas Gaj, Charles A Gersbach, Carlos F Barbas
Trends in Biotechnology 2013-07-01
Zinc-finger nucleases (ZFNs) and transcription activator-like effector nucleases (TALENs) comprise a powerful class of tools that are redefining the boundaries of biological research. These chimeric nucleases are composed of programmable, sequence-specific DNA-binding modules linked to a nonspecific DNA cleavage domain. ZFNs and...Read More
R W Farndale, D J Buttle, A J Barrett
Biochimica et Biophysica Acta 1986-09-04
The dimethylmethylene blue assay for sulphated glycosaminoglycans has found wide acceptance as a quick and simple method of measuring the sulphated glycosaminoglycan content of tissues and fluids. The available assay methods have lacked specificity for sulphated glycosaminoglycans in the presence of other polyanions, however, an...Read More
Laurie Delalande, Denis Faure, Aurélie Raffoux, Stéphane Uroz, Cathy D'Angelo-Picard, Miena Elasri, Aurélien Carlier, Romain Berruyer, Annik Petit, Paul Williams, Yves Dessaux
FEMS Microbiology Ecology 2005-03-01
The half-life of N-hexanoyl-l-homoserine lactone (C6-HSL) was determined under various pH and temperature conditions, and in several plant environments. C6-HSL was sensitive to alkaline pH, a process that was also temperature-dependent. In addition, C6-HSL disappeared from plant environments, i.e. axenic monocot and dicot plants...Read More

January 2015

Ibuprofen can extend lifespans

Ibuprofen has been used as an anti-inflammatory treatment for many decades. Inflammation has been a known to affect an organism’s lifespan. A comprehensive study of multiple organisms (yeast, fly, and worm) has shown that the lifetime of those organisms increased significantly when dosed with Ibuprofen. Ibuprofen prevented the transportation of the amino acid Tyrptophan in the animal models, and that has been shown to be involved in the lifespan extensions. While human trials have not yet been performed, it is interesting that Ibuprofen could have more cellular effects than just anti-inflammation.

Paper

Chong He, Scott K Tsuchiyama, Quynh T Nguyen, Ekaterina N Plyusnina, Samuel R Terrill, Sarah Sahibzada, Bhumil Patel, Alena R Faulkner, Mikhail V Shaposhnikov, Ruilin Tian, Mitsuhiro Tsuchiya, Matt Kaeberlein, Alexey A Moskalev, Brian K Kennedy, Michael Polymenis
PLoS Genetics 2014-12-01
The common non-steroidal anti-inflammatory drug ibuprofen has been associated with a reduced risk of some age-related pathologies. However, a general pro-longevity role for ibuprofen and its mechanistic basis remains unclear. Here we show that ibuprofen increased the lifespan of Saccharomyces cerevisiae, Caenorhabditis elegans a...Read More

Hot Papers on Sigma-Aldrich.com

Here are 5 of the most trending papers on Sigma-Aldrich.com in December. These hot papers have helped our customers further their research. Sigma-Aldrich provides both a wide array of life-science and chemical products along with a wide-array of peer-reviewed papers.

Florence Teulé, Alyssa R Cooper, William A Furin, Daniela Bittencourt, Elibio L Rech, Amanda Brooks, Randolph V Lewis
Nature Protocols 2009-01-01
The extreme strength and elasticity of spider silks originate from the modular nature of their repetitive proteins. To exploit such materials and mimic spider silks, comprehensive strategies to produce and spin recombinant fibrous proteins are necessary. This protocol describes silk gene design and cloning, protein expression in...Read More
Zunfang Hu, Longqian Xu, Xianghua Wen
Journal of Environmental Sciences 2013-01-01
Immobilization of enzymes on mesoporous silicas (MS) allows for good reusability. MS with two-dimensional hexagonal pores in diameter up to 14.13 nm were synthesized using Pluronic P123 as template and 1,3,5-triisopropylbenzene as a swelling agent in acetate buffer. The surface of MS was modified by the silanization reagents 3-a...Read More
Hongyan Zhao, Feng Song, Fengxiao Wang, Jiadong Liu, Yanling Liu, Jun Zhang, Guiyang Chen, Shujing Liu
Journal of Nanoscience and Nanotechnology 2014-06-01
The influence of 12 nm spherical silver nanoparticles (AgNPs) and 20 nm spherical gold nanoparticles (AuNPs) on the luminescence of europium complex Eu(TTFA)3 were studied. When 350 nm is chosen as the incident light, the maximum enhancement factor of the complexes mixed with AgNPs is about 2.5 at the wavelength of 612 nm. Besid...Read More
Marieke Veurink, Yvonne Westermaier, Robert Gurny, Leonardo Scapozza
Pharmaceutical Research 2013-04-01
To investigate the mechanism behind the aggregation breaking properties of dexamethasone phosphate and related corticosteroids on the IgG1 antibody bevacizumab (Avastin®). An in silico 3D dimer model is developed to identify the bevacizumab-bevacizumab interface, and different corticosteroids are docked onto the model to disting...Read More
Akram Ghantous, Ansam Sinjab, Zdenko Herceg, Nadine Darwiche
Drug Discovery Today 2013-09-01
Parthenolide (PTL), a sesquiterpene lactone (SL) originally purified from the shoots of feverfew (Tanacetum parthenium), has shown potent anticancer and anti-inflammatory activities. It is currently being tested in cancer clinical trials. Structure-activity relationship (SAR) studies of parthenolide revealed key chemical propert...Read More

December 2014

Novel Lyme Disease Vaccine Developed

Currently there is no widely available Lyme disease vaccine for people. Recently a vaccine against many different Outer surface protein A (OspA) serotypes has been developed. This vaccine was tested in mice and the efficacy was found to be better than the previously available LYMErix vaccine. The coverage of this new vaccine approach should help it become a viable solution to Lyme Borreliosis that is prevalent in both Europe and the Americas.

Paper

Pär Comstedt, Markus Hanner, Wolfgang Schüler, Andreas Meinke, Urban Lundberg
PLoS ONE 2014-01-01
There is currently no Lyme borreliosis vaccine available for humans, although it has been shown that the disease can be prevented by immunization with an OspA-based vaccine (LYMErix). Outer surface protein A (OspA) is one of the dominant antigens expressed by the spirochetes when present in a tick...Read More

Hot Papers on Sigma-Aldrich.com

Here are 5 of the most trending papers on Sigma-Aldrich.com in November. Sigma-Aldrich provides both a wide array of life-science and chemical products along with a wide-array of peer-reviewed papers.

Lauren Phillips, Mike A Singer
Neurology 2013-02-19
The benzene-based compound 2,4-dinitrophenol was developed in the late 19th century, and was used during World War I for manufacture of explosives.(1) A number of cases of dinitrophenol poisoning were noted in French munitions plant workers. Acute inisxication caused hyperpyrexia, nausea, vomiting, and diarrhea; sympisms of suba...Read More
Robert A Koeth, Zeneng Wang, Bruce S Levison, Jennifer A Buffa, Elin Org, Brendan T Sheehy, Earl B Britt, Xiaoming Fu, Yuping Wu, Lin Li, Jonathan D Smith, Joseph A DiDonato, Jun Chen, Hongzhe Li, Gary D Wu, James D Lewis, Manya Warrier, J Mark Brown, Ronald M Krauss, W H Wilson Tang, Frederic D Bushman, Aldons J Lusis, Stanley L Hazen
Nature Medicine 2013-05-01
Intestinal microbiota metabolism of choline and phosphatidylcholine produces trimethylamine (TMA), which is further metabolized to a proatherogenic species, trimethylamine-N-oxide (TMAO). We demonstrate here that metabolism by intestinal microbiota of dietary L-carnitine, a trimethylamine abundant in red meat, also produces TMAO...Read More
Kevin S Thorneloe, Mui Cheung, Weike Bao, Hasan Alsaid, Stephen Lenhard, Ming-Yuan Jian, Melissa Costell, Kristeen Maniscalco-Hauk, John A Krawiec, Alan Olzinski, Earl Gordon, Irina Lozinskaya, Lou Elefante, Pu Qin, Daniel S Matasic, Chris James, James Tunstead, Brian Donovan, Lorena Kallal, Anna Waszkiewicz, Kalindi Vaidya, Elizabeth A Davenport, Jonathan Larkin, Mark Burgert, Linda N Casillas, Robert W Marquis, Guosen Ye, Hilary S Eidam, Krista B Goodman, John R Toomey, Theresa J Roethke, Beat M Jucker, Christine G Schnackenberg, Mary I Townsley, John J Lepore, Robert N Willette
Science Translational Medicine 2012-11-07
Pulmonary edema resulting from high pulmonary venous pressure (PVP) is a major cause of morbidity and mortality in heart failure (HF) patients, but current treatment options demonstrate substantial limitations. Recent evidence from rodent lungs suggests that PVP-induced edema is driven by activation of pulmonary capillary endoth...Read More
Le Cong, F Ann Ran, David Cox, Shuailiang Lin, Robert Barretto, Naomi Habib, Patrick D Hsu, Xuebing Wu, Wenyan Jiang, Luciano A Marraffini, Feng Zhang
Science 2013-02-15
Functional elucidation of causal genetic variants and elements requires precise genome editing technologies. The type II prokaryotic CRISPR (clustered regularly interspaced short palindromic repeats)/Cas adaptive immune system has been shown to facilitate RNA-guided site-specific DNA cleavage. We engineered two different type II...Read More

November 2014

Novel protein found to be diabetes treatment

A recent study in Nature has found that Fibroblast growth factor 1 (FGF1) has a positive benefit in lowering high blood sugar levels in mice. This is an important step in finding a safer protein based drug that can lower high sugar levels before or after type 2 diabetes forms in people. This early step still needs to be validated in humans but it is an important step to help prevent and treat the epidemic of type 2 diabetes.

Paper

Jae Myoung Suh, Johan W Jonker, Maryam Ahmadian, Regina Goetz, Denise Lackey, Olivia Osborn, Zhifeng Huang, Weilin Liu, Eiji Yoshihara, Theo H van Dijk, Rick Havinga, Weiwei Fan, Yun-Qiang Yin, Ruth T Yu, Christopher Liddle, Annette R Atkins, Jerrold M Olefsky, Moosa Mohammadi, Michael Downes, Ronald M Evans
Nature 2014-09-18
Fibroblast growth factor 1 (FGF1) is an autocrine/paracrine regulator whose binding to heparan sulphate proteoglycans effectively precludes its circulation. Although FGF1 is known as a mitogenic factor, FGF1 knockout mice develop insulin resistance when stressed by a high-fat diet, suggesting a potential role in nutrient homeost...Read More

Genes

Hot Papers on Sigma-Aldrich.com

Here are 5 of the most trending papers on Sigma-Aldrich.com in October. Sigma-Aldrich provides both a wide array of life-science and chemical products along with a wide-array of peer-reviewed papers.

Kristina Martinez, Shruthi Shyamasundar, Arion Kennedy, Chia Chi Chuang, Angel Marsh, Jennifer Kincaid, Tanya Reid, Michael McIntosh
Journal of Lipid Research 2013-03-01
Diacylglycerol kinases (DGK) convert diacylglycerol to phosphatidic acid, which has been reported to stimulate calcium release from the endoplasmic reticulum. Based on our published data showing that trans-10, cis-12 conjugated linoleic acid (t10,c12 CLA)-mediated intracellular calcium accumulation is linked to inflammation and ...Read More
Kensuke Osada, Horacio Cabral, Yuki Mochida, Sangeun Lee, Kazuya Nagata, Tetsuya Matsuura, Megumi Yamamoto, Yasutaka Anraku, Akihiro Kishimura, Nobuhiro Nishiyama, Kazunori Kataoka
Journal of the American Chemical Society 2012-08-15
Spontaneous formation of polymeric metallosomes with uniform size (~100 nm) was found to occur in aqueous medium through the reaction of an anticancer agent, (1,2-diaminocyclohexane)platinum(II) (DACHPt), with a Y-shaped block copolymer of ω-cholesteroyl-poly(L-glutamic acid) and two-armed poly(ethylene glycol) (PEGasus-PLGA-Cho...Read More
E V Siloto, E F A Oliveira, J R Sartori, V B Fascina, B A B Martins, D R Ledoux, G E Rottinghaus, D R S Sartori
Poultry Science 2013-08-01
Aflatoxins (AF) and fumonisins (FU) are a major problem faced by poultry farmers, leading to huge economic losses. This experiment was conducted to determine the effects of AF (1 mg/kg of feed) and FU (25 mg/kg of feed), singly or in combination, on the lipid metabolism in commercial layers and investigate the efficacy of a comm...Read More
Md Rafique Ahmed, Mohammad Anis
Applied Biochemistry and Biotechnology 2012-11-01
The effect of thidiazuron (TDZ) has been investigated in shoot multiplication for a simple, efficient, rapid, and commercially applicable regeneration protocol of an important medicinal plant, Vitex trifolia. Multiple shoots were induced in nodal explants obtained from a mature tree on Murashige and Skoog (MS) medium supplemente...Read More
Atul Bhardwaj, Jatinder Kaur, Frank Wuest, Edward E Knaus
Chemmedchem 2014-01-01
A group of cyclooxygenase-2 (COX-2)-specific fluorescent cancer biomarkers were synthesized by linking the anti-inflammatory drugs ibuprofen, (S)-naproxen, and celecoxib to the 7-nitrobenzofurazan (NBD) fluorophore. In vitro COX-1/COX-2 inhibition studies indicated that all of these fluorescent conjugates are COX-2 inhibitors (I...Read More

October 2014

Plant drought tolerance increased by suppressing ESK1 gene

With the ever present concern over global climate change, the creation of genetically altered crops that are immune to drought cycles have become an important goal. The ESK1 gene has been known to be involved in plant survivability in frost and freezing conditions. A recent study by Fuhui Xu et. al has discovered that silencing ESK1 can also help increase drought tolerance in Arabidopsis thaliana plants. By Silencing the ESK1 gene with siRNA and over expressing the stress response genes ICE1, CBF1, and CBF3 they found that drought tolerance has significantly increased. Using this information to study the effects in other pants will help further elucidate the ESK1 genes role in drought resistance.

Paper

Fuhui Xu, Zhixue Liu, Hongyan Xie, Jian Zhu, Juren Zhang, Josef Kraus, Tasja Blaschnig, Reinhard Nehls, Hong Wang
PLoS ONE 2014-01-01
Improved drought tolerance is always a highly desired trait for agricultural plants. Significantly increased drought tolerance in Arabidopsis thaliana (Columbia-0) has been achieved in our work through the suppression of ESKMO1 (ESK1) gene expression with small-interfering RNA (siRNA) and overexpression of CBF genes with constitutive gene expression...Read More

Hot Papers on Sigma-Aldrich.com

Here are 5 of the most trending papers on Sigma-Aldrich.com in September. Sigma-Aldrich provides both a wide array of life-science and chemical products along with a wide-array of peer-reviewed papers.

J S JS Elmendorf, D D Chen, J E JE Pessin
Journal of Biological Chemistry 1998-05-22
Guanosine 5'-O-(3-thiotriphosphate) (GTPgammaS) treatment of permeabilized adipocytes results in GLUT4 translocation similar to that elicited by insulin treatment. However, although the selective phosphatidylinositol 3-kinase inhibitor, wortmannin, completely prevented insulin-stimulated GLUT4 translocation, it was without effect on GTPgammaS-stimulated GLUT4 translocation...Read More
Teresa Cirillo, Evelina Fasano, Francesco Esposito, Paolo Montuori, Renata Amodio Cocchieri
Food and Chemical Toxicology 2013-01-01
Ready-to-eat packed meals intended to hospital patients were studied over a two-weeks period to measure the contents of di-(2-ethylhexyl) phthalate (DEHP) and di-n-butylphthalate (DBP) and to evaluate their daily intake by total diet. The packaging consisted of polyethylene terephthalate (PET) dishes sealed with polypropylene (PP) foil....Read More
Elham Raeisi, Lluis M Mir
Journal of Membrane Biology 2012-10-01
This study investigated whether molecules spontaneously transported inside cells, like glucose derivatives, can also be used as electropermeabilization markers. Uptake of a fluorescent deoxyglucose derivative (2-NBDG) by normal and electropermeabilized cells in culture was analyzed...Read More
Rizwan Wahab, Nagendra Kumar Kaushik, Neha Kaushik, Eun Ha Choi, Ahmad Umar, Sourabh Dwivedi, Javed Musarrat, Abdulaziz A Al-Khedhairy
Journal of Biomedical Nanotechnology 2013-07-01
This paper reports the synthesis and characterization of ZnO nanoparticles prepared by soft chemical process. The nanoparticles of ZnO possess wurtzite hexagonal phase and were used for the induction of cell death in malignant human T98G gliomas, KB epithermoids and HEK normal non-malignant kidney cells...Read More
Sung Park, Gil Rak Choi, Youn Cheol Kim, Jae Chun Lee, Ju Hyeon Lee
Journal of Nanoscience and Nanotechnology 2013-05-01
A unique synthesis method was developed, which is called solution combustion method (SCM). TiO2 nanopowder was synthesized by this method. This SCM TiO2 nanopowder (-35 nm) was added to the dielectric layer of AC powder electroluminescence (EL) device...Read More

September 2014

PALB2 Gene as a Breast Cancer Biomarker

A recent study in The New England Journal of Medicine has shown that the gene PALB2 is just as an important marker for Breast Cancer as is BRCA2. A loss of function mutation in PALB2 was known as a cancer risk, however the risk over time was not well defined. By analyzing familial genetic screenings, this paper shows the risk of breast cancer was 8 to 9 times higher for 40 to 60 year olds with PALB2 mutations when compared to the general population. Genetic screenings will continue to become a more powerful diagnostic tool as scientists discover and define biomarkers.

Paper

Antonis C Antoniou, et al.
New England Journal of Medicine 2014-08-07
Germline loss-of-function mutations in PALB2 are known to confer a predisposition to breast cancer. However, the lifetime risk of breast cancer that is conferred by such mutations remains unknown.We analyzed the risk of breast cancer among 362 members of 154 families who had deleterious truncating, splice, or deletion mutations in PALB2...Read More


Genes

Hot Papers on Sigma-Aldrich.com

Here are 5 of the most trending papers on Sigma-Aldrich.com in August. Sigma-Aldrich provides both a wide array of life-science and chemical products along with a wide-array of peer-reviewed papers.

Hynda K Kleinman, George R Martin
Seminars in Cancer Biology 2005-01-01
The basement membrane extracellular matrix contacts epithelial, endothelial, fat and smooth muscle cells. Because this extracellular matrix is so thin, it had been hard to study its composition, structure, and function. An extract of a tumor was found to contain all of the components present in basement and to be very biologically active...Read More
Andrew B Jackson, et al.
Nuclear Medicine and Biology 2012-04-01
The present study describes the design and development of a new heterodimeric RGD-bombesin (BBN) agonist peptide ligand for dual receptor targeting of the form (64)Cu-NO2A-RGD-Glu-6-Ahx-BBN(7-14)NH(2) in which Cu-64=a positron emitting radiometal; NO2A=1,4,7-triazacyclononane-1,4-diacetic acid; Glu=glutamic acid; 6-Ahx=6-aminohexanoic acid; RGD=the amino acid sequence [Arg-Gly-Asp], a nonregulatory peptide that has been used extensively to target α(v)β(3) receptors up-regulated on tumor cells...Read More
Benjamin C Jennings, et al.
Journal of Lipid Research 2009-02-01
Pharmacologic approaches to studying palmitoylation are limited by the lack of specific inhibitors. Recently, screens have revealed five chemical classes of small molecules that inhibit cellular processes associated with palmitoylation (Ducker, C. E., L. K. Griffel, R. A. Smith, S. N. Keller, Y. Zhuang, Z. Xia, J. D. Diller, and C. D. Smith. 2006...Read More
Thomas Decker, Marie-Luise Lohmann-Matthes
Journal of Immunological Methods 1988-01-01
A simple way of measuring and evaluating lactate dehydrogenase release from lysed tumor cells is described. LDH activity was determined as NADH oxidation or INT reduction over a defined time interval, which was limited by stopping the enzymatic reaction with the inhibitor oxamate. Reaction products were then assayed using a microplate reader...Read More
Zahid H Siddik
Oncogene 2003-10-20
Cisplatin is one of the most potent antitumor agents known, displaying clinical activity against a wide variety of solid tumors. Its cytotoxic mode of action is mediated by its interaction with DNA to form DNA adducts, primarily intrastrand crosslink adducts, which activate several signal transduction pathways, including those involving ATR, p53, p73, and MAPK, and culminate in the activation of apoptosis...Read More