True Gel3D™ Troubleshooting guide

Issues Reasons Solutions
Weak/low gel formation Thiol-reactive groups (polymer) and thiol groups (crosslinker) are not near equimolar concentration Balance the thiol-reactive groups of polymer with thiol groups of crosslinkers
Excess of peptide with thioglycerol results in few thiol-reactive groups Balance the thiol groups of peptide and crosslinker with thiol-reactive groups of polymer
Low pH caused by acid in peptide sample Optimize the buffer pH: For a slow gelling system use a buffer with pH 7.2. For a fast gelling system use a buffer with pH 5.5
Weak network formed because of deteriorated reactive groups Use fresh aliquots 
Fast gelation pH is too high Use buffer with pH 5.5 to slow down the gelation
Too slow gelation process pH is too slow Use buffer with pH 7.2 to accelerate the gelation
Decrease in cell viability Lack of adhesion factor in polymer Add appropriate adhesion peptide or proteins
Acidic environment Use neutral pH during gel setup
Incomplete dissolution of dextran polymer even after 1 hour incubation with TrueGel3DTM enzymatic cell recovery solution High gel strength Increase the incubation period for 2 hours and increase concentration of TrueGel3DTM enzymatic cell recovery solution
Reduced TrueGel3DTM enzymatic cell recovery solution activity Use fresh aliquot of enzyme

Materials