KitAlysis™ High-Throughput Base Reaction Screening Kit

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Materials Included in your KITALYSISBASE-2PK High-Throughput Screening Kit

Contents in each of the 2 individually sealed Mylar (foil bags):

  • 22 (11 x 2) pre-weighed bases in glass vials loaded with stir bars, topped with cap mat.
  • 4 empty 4 mL reaction vials

The screening sets come pre-loaded with 30 µmol of base in each vial according to the following design.


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Product No. Description Vial
RDD019 K3PO4 A1, C1
900501 K2CO3 A2, C2
791733 KOAc A3, C3
237205 KHCO3 A4, C4
757551 KOH A5, C5
795429 NaOH A6, C6
441902 Cs2CO3 B1, D1
225770 LiHMDS 1.0 M in THF B2, D2
702706 NaOtBu 2.0 M in THF B3, D3
139009 DBU B4, D4
900632 Et3N B5, D5
n/a Chemist’s Choice B6, D6

All contents in the foil bag are weighed, plated, packed, and sealed in a glove box under nitrogen.

Ampule Boxes:

  • 2 (2 mL) ampule each of the following: DBU, Et3N, LiHMDS, NaOtBu
  • 4 (2 mL) ampule each of the following: DMF, MeOH, THF, Toluene

KitAlysis™ 24-well Reaction Block Replacement Films-2EA

  • Pack of 2 enables a new film to be used with each kit ensuring a tight, cross-contaminate-free seal every time.

Stir Bars-8 individually packed

  • Fit perfectly into supplied vials to ensure proper stirring of substrate mixtures.

Additional Recommended Materials

Product No. Description
Z688711 96-well plate for automated HPLC analysis
C1111 96-well plate cap mat for automated HPLC analysis
Z683809 10-100 uL pipette
Z640220 2-200 uL pipette tip refill
Z683825 100-1000 uL pipette
Z640247 50-1000 uL pipette tip refill
Z192570 2 inch needles for ease in ampule solvent extraction
Z230723 1 mL needle for accurate solvent volume extraction from ampule

Kit Design

The base kit was designed to provide the best possible chance of finding good reaction conditions for cross-coupling reactions:

  • pre-weighed base or base solution (provided)
  • 10–20 mmol coupling partner (user supplied)
  • 10 mmol aryl halide substrate (user supplied)
  • catalyst and ligand  (user supplied).
  • 4 solvents (DMF, MeOH, THF, and Toluene) (provided)

Use of the Provided Tools

Multiple tools have been created to ensure your success with kit set up. Start with the more detailed guide to ensure you are comfortable with all of the steps before using the quick guides on the excel worksheet. Remember that while the technique is new, it is still organic chemistry and so the steps will seem easy once you try just one kit. It is just a new way of approaching something you are already very good at.

Detailed Set-Up User Guide:

Designed for the first time user and should be read completely before getting started. Best if used in conjunction with the video as not all steps are outlined in the video in great detail. This guide includes trouble-shooting tips, how-to’s for the Labware, and work-up recipes with procedures. Everything you need to set up a kit with confidence every time.

Excel Sheet:

Each sheet is designed to be used with the specific experimental design chosen by you depending upon the attributes of your substrates. The downloadable excel files are specific to the kit being run and can be found within each Step-by-Step User Guide.

They have the following features:

  • Calculations for substrate recipes depending upon the molecular weight of your substrates
  • Quick directions for the more avid user
  • Print button to allow you to take recipe to the lab
  • Contain all info such that they can be can be saved as a pdf and appended to ELN for experimental information