KitAlysis™ High-Throughput Miyaura Borylation Reaction Screening Kit

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Materials Included in your KitAlysis™ High-Throughput Miyaura Borylation Screening Kit

Contents in each of the 2 individually sealed Mylar (foil bags):

  • 22 (11 x 2) pre-weighed bases in glass vials loaded with stir bars, topped with cap mat.
  • 4 empty reaction vials with lids (to make stock solutions)

The screening sets come pre-loaded with 30 µmol of base in each vial according to the following design.

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Product No. Description Vial
804282 SPhos Pd G4 A1, B1, C1, D1
804274 XPhos Pd G4 A2, B2, C2, D2
900349 CataCXium Pd G4 A3, B3, C3, D3
804290 RuPhos Pd G4 A4, B4, C4, D4
764175 PCy3 Pd G4 A5, B5, C5, D5
804975 DTBPF Pd G A6, B6, C6, D6

All contents in the foil bag are weighed, plated, packed, and sealed in a glove box under nitrogen.

Ampule Boxes:

  • 4 empty 4 mL reaction vials
  • 2 (2 mL) ampule each of the following: ethylene glycol, DIPEA
  • 4 (2 mL) ampule each of the following: toluene, DMA, THF, 1-butanol. MEOH, MECN

Internal Standard:

  • Biphenyl (30mg). To be added to the reaction during the work-up. Recipe for the work-up with the internal standard can be found within the step-by-step guide

KitAlysis™ 24-well Reaction Block Replacement Films-2EA

  • Pack of 2 enables a new film to be used with each kit ensuring a tight, cross-contaminate-free seal every time.

Stir Bars-8 individually packed

  • Fit perfectly into supplied vials to ensure proper stirring of substrate mixtures.

Additional Recommended Materials

Product No. Description
Z688711 96-well plate for automated HPLC analysis
C1111 96-well plate cap mat for automated HPLC analysis
Z683809 10-100 uL pipette
Z640220 2-200 uL pipette tip refill
Z683825 100-1000 uL pipette
Z640247 50-1000 uL pipette tip refill
Z192570 2 inch needles for ease in ampule solvent extraction
Z230723 1 mL needle for accurate solvent volume extraction from ampule

Kit Design

The Miyaura Borylation Kit was designed to provide the best possible chance of finding good reaction conditions for cross-coupling reactions:

  • pre-weighed base or base solution (provided)
  • 10–20 mmol coupling partner (user supplied)
  • 10 mmol aryl halide substrate (user supplied)
  • Catalysts (user supplied).
  • 6 solvents (toluene, DMA. THJF, n-butanol, MeOH, MeCN) (provided)

Use of the Provided Tools

Multiple tools have been created to ensure your success with kit set up. Start with the more detailed guide to ensure you are comfortable with all of the steps before using the quick guides on the excel worksheet. Remember that while the technique is new, it is still organic chemistry and so the steps will seem easy once you try just one kit. It is just a new way of approaching something you are already very good at.

Detailed Set-Up User Guide:

Designed for the first time user and should be read completely before getting started. Best if used in conjunction with the video as not all steps are outlined in the video in great detail. This guide includes trouble-shooting tips, how-to’s for the Labware, and work-up recipes with procedures. Everything you need to set up a kit with confidence every time.

Excel Sheet:

Each sheet is designed to be used with the specific experimental design chosen by you depending upon the attributes of your substrates. The downloadable excel files are specific to the kit being run and can be found within each Step-by-Step User Guide.

They have the following features:

  • Calculations for substrate recipes depending upon the molecular weight of your substrates
  • Quick directions for the more avid user
  • Print button to allow you to take recipe to the lab
  • Contain all info such that they can be can be saved as a pdf and appended to ELN for experimental information

Materials

     
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