KitAlysis™ High-Throughput Copper C-N Cross-Coupling Reaction Screening Kit

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Materials Included in your KITALYSIS-CN-2PK High-Throughput Screening Kit

Contents in each of the 2 individually sealed Mylar (foil bags):

  • 24 (6 x 4) pre-weighed ligands in glass vials loaded with stir bars, topped with cap mat.

The screening sets come pre-loaded with 4 µmol of ligand in each vial according to the following design.

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Description Product No. Vial
2,6-Dimethylanilino(oxo)acetic acid 806412 A1, B1, C1, D1
2,6-Dimethoxyanilino(oxo)acetic acid
A2, B2, C2, D2
2,6-Difluoroanilino(oxo)acetic acid 806439 A3, B3, C3, D3
2,3,4,5,6-Pentafluoroanilino(oxo)acetic acid 806447 A4, B4, C4, D4
3,5-Bis(trifluoromethyl)anilino(oxo)acetic acid
A5, B5, C5, D5
2-Fluoro-6-(piperidine-1-sulfonyl)anilino(oxo)acetic acid
A6, B6,C6, D6
  • Potassium phosphate: 4 pre-weighed vials with product labels and lids
  • CuI (soluble source) in pre-weighed vial

All contents in the foil bag are weighed, plated, packed, and sealed in a glove box under nitrogen.

Ampule Boxes:

  • Dioxane: 4 x 2 mL of degassed, anhydrous Aldrich® Sure/Seal™
  • DMAc: 4 x 2 mL of degassed, anhydrous Aldrich® Sure/Seal™
  • Toluene: 4 x 2 mL degassed anhydrous Aldrich Sure/Seal™
  • DMSO: 4 x 2 mL degassed anhydrous Aldrich Sure/Seal™

Internal Standard:

  • Biphenyl (30 mg). To be added to the reaction during the work-up. Recipe for the work-up with the internal standard can be found within the hyperlinked screen types in the "Kit Design" section below.

KitAlysis 24-well Reaction Block Replacement Films-2EA

  • Pack of 2 enables a new film to be used with each kit ensuring a tight, cross-contaminate-free seal every time.

Stir Bars-8 individually packed

  • Fit perfectly into supplied vials to ensure proper stirring of substrate mixtures.

Additional Recommended Materials (Sold Separately)

  • Z688711: 96-well plate for automated HPLC analysis
  • C1111: 96-well plate cap mat for automated HPLC analysis
  • Z683809: 10-100 uL pipette
  • Z640220: 2-200 uL pipette tip refill
  • Z683825: 100-1000 uL pipette
  • Z640247: 50-1000 uL pipette tip refill
  • Z192570: 2 inch needles for ease in ampule solvent extraction
  • Z230723: 1 mL needle for accurate solvent volume extraction from ampule

Kit Design

Cu C-N (Buchwald-Hartwig) cross-coupling kit was designed to provide the best possible chance of success and is run with:

  • 10 µmol aryl halide
  • 15 µmol amine (primary, secondary), use 20 µmol if the material is not too precious
  • 4 µmol ligand per vial (1:4, Cu:Ligand); 6 in this system (40 mol%)
  • 30 µmol base (K3PO4)
  • 0.1 M solvent concentration (Dioxane, DMAc, Toluene, & DMSO)
  • 1 µmol CuI (soluble source, 10 mol%)

Use of the Provided Tools

Multiple tools have been created to ensure your success with kit set up. Start with the more detailed guide to ensure you are comfortable with all of the steps before using the quick guides on the excel worksheet. Remember that while the technique is new, it is still organic chemistry and so the steps will seem easy once you try just one kit. It is just a new way of approaching something you are already very good at.

Detailed Set-Up User Guide:
Designed for the first time user and should be read completely before getting started. Best if used in conjunction with the video as not all steps are outlined in the video in great detail. This guide includes trouble-shooting tips, how-to’s for the Labware, and work-up recipes with procedures. Everything you need to set up a kit with confidence every time.

Excel Sheet:
Each sheet is designed to be used with the specific experimental design chosen by you depending upon the attributes of your substrates. The downloadable excel files are specific to the kit being run and can be found within each Step-by-Step User Guide. They have the following features:

  • Calculations for substrate recipes depending upon the molecular weight of your substrates
  • Quick directions for the more avid user
  • Print button to allow you to take recipe to the lab
  • Contain all info such that they can be can be saved as a pdf and appended to ELN for experimental information