Agarose Beads – Recombinant Proteins

Ni-NTA His•Bind® Resin is a high-performance Ni2+-charged agarose used for rapid, one-step purification of proteins containing a polyhistidine tag sequence.

S-protein Agarose specifically retains S•Tag™ fusion proteins. Binding can be performed in a column or batch mode. Elution is possible using slightly denaturing conditions or using a specific protease, such as thrombin or enterokinase, with appropriate recombinant proteins.

A crude extract containing unfused GST was applied to a 2-mL GST-Bind Resin column.
Chemical structure of Ni-NTA His•Bind® resin shows basis for high capacity binding of His-tagged proteins.