N-Hydroxyethyl acrylamide — Tool for Lab-On-A-Chip Research - Material Matters Volume 3 Issue

By: Joe Porwoll, Material Matters Volume 3 Issue 3

Joe Porwoll, President Aldrich Chemical Co., Inc.

Do you have a compound that you wish Sigma-Aldrich® could list to help materials research? If it is needed to accelerate your research, it matters—please send your suggestion to matsci@sial.com and we will be happy to give it careful consideration.

N-Hydroxyethyl acrylamide — Tool for Lab-On-A-Chip Research

Professor Annelise Barron of Stanford University kindly suggested that we offer N-hydroxyethyl acrylamide (HEAA), a monomer which can be used to make polymers that are useful as wall coatings and separation media for lab-on-achip devices. Hydrophilic poly-N-hydroxyacrylamide (pHEAA), synthesized by free radical polymerization of HEAA in water, forms a stable adsorbed coating on glass and fused silica microchannels, enabling excellent biomolecule separations. pHEAA coatings eliminate electroosmotic flow and greatly reduce non-specifc adsorption of biomolecules to the internal walls of microfluidic channels. DNA fragments of > 500 bases have been successfully separated and sequenced using pHEAA-assisted microcapillary electrophoresis.1,2

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  1. Albarghouthi, M.N., Buchholtz, B.A., Huiberts, P.J., Stein, T.M., Barron, A.E. Electrophoresis 2002, 23, 1429.
  2. Fredlake, C.P., Hert, D.G., Kan, C.W., Chiesl, T.N., Root, B.E., Forster, R.E., Barron, A.E. Proc. Natl. Acad. Sci. USA 2008, 105, 476.

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