EZ-Fluo Rapid Microbial Detection System – Data Sheet

EZ-Fluo™ Rapid Detection SystemA fast, non-destructive, fluorescent staining-based system for microbial detection

  • Rapid results in one third of the time in comparison to traditional techniques
  • Flexible approach based on standard membrane filtration with reduced incubation time on media plate
  • Non-destructive method—compatible with any ID technique
  • Compact hardware, fits easily on any laboratory bench
  • Cost effective solution for routine use as well as investigation

Easy to integrate into any laboratory

Our EZ-Fluo Rapid Detection System enables you to significantly reduce your time to result, address contamination events sooner, and therefore, gain better control of your manufacturing process. The EZ-Fluo System is a rapid florescent-based technology designed for fast quantitative detection of microorganisms over a broad range of filterable samples. This new system uses standard membrane filtration to detect viable and culturable microorganisms down to 1 CFU per sample. EZ-Fluo test results are easily comparable to compendial method test results, which facilitates the validation and the integration of this rapid system in any laboratory without causing disruption. Detected microbial contamination can be then identified using your current ID methodology.

Faster product release

Today, more and more QC laboratories are switching to rapid methods for the detection of microbial contamination. This is because traditional methods require several days to provide results and therefore are of limited value when it comes to releasing product to the market faster, or to conducting quick investigations when necessary.

Simple protocol using regular membrane filtration and incubation

The EZ-Fluo Rapid Detection System consists of a reader and staining reagents used in combination with regular 47 mm filtration membranes and standard media. Optimal results can be obtained using MilliporeSigma’s membranes and comprehensive media portfolio. The use of the camera (optional) offers traceability of results and comfort when reading.

1. Sample preparation

Filtration using EZ-Fit® Filtration Unit and media plates: Simply filter your sample the same way you prepare your current samples and incubate the membrane on your regular media type.



2. Fluorescent staining

Transfer membrane to a Petri-Pad Petri dish wetted with staining reagent and incubate for 30 minutes.


Datasheet Fluorescent Staining

3. Counting CFUs

Count fluorescent colonies through the window of the EZ-Fluo Reader or use the camera (optional) to view the colonies on your computer screen.

Datasheet Counting CFUs


The EZ-Fluo system can detect and quantify microbial contamination in any filterable sample. Potential applications are numerous and include testing of the following sample types:

  • Raw materials
  • In-process samples
  • Final product
  • Environmental samples

Reincubation for microorganism identification

The EZ-Fluo System is a non-destructive method that enables you to continue to grow the microorganisms after they have been stained, in order to identify them using any standard ID technology. This is a tremendous advantage compared to other rapid test systems that do not allow ID of microorganisms.

Reincubation step

Place membrane on a prefilled agar media cassette and reincubate. Collect and isolate the microorganisms and identify using any existing ID methodology.

Datasheet Reincubation Step

Example: Tested sample of in-process, non-sterile water using the EZ-Fluo system. After detection, membrane was re-incubated for full growth and identification.

Non-destructive technology

The EZ-Fluo method is based on a unique, non-destructive, fluorescent marker developed by MilliporeSigma. After filtration and incubation, the staining reagent is applied, and any viable and culturable microorganisms are stained. The reagent is initially non-fluorescent, but an intracellular enzyme that is active only as part of the metabolic processes of livingcells, cleaves the substrate to liberate the fluorophore.

As fluorophore accumulates inside the cells, the signal is naturally amplified. In the EZ-Fluo reader, the cells are exposed to the excitation wavelength of the fluorescent dye so that they can be visually counted. The fluorescence allows much smaller colonies to be seen than are visible by the naked eye.

Principle of fluorescent staining

Datasheet Principle of Fluorescent Staining


Datasheet Specifications

Support and services

We provide a comprehensive range of support and services to help you save your QC resources and facilitate the implementation of your EZ-Fluo™ system into your daily testing routine. Our highly experienced service teams can assist you with the following activities:

  • Feasibility study and on-site evaluation
  • Method development and method development consulting
  • Equipment installation
  • Operator training
  • On-site system qualification (IQ/OQ)
  • Yearly preventive maintenance
  • Service plan

For further information on our support and services offering, please contact your local sales representative.