New IFU Method No. 12:2019

BAT broth and BAT agar acc. IFU No. 12:2019 for detection and enumeration of Alicyclobacillus spp. in juice and juice-related products and their ingredients intended for human consumption.

The International Fruit and Vegetable Juice Association (IFU) published a revised Method No. 12 standard for detection and enumeration of spore-forming, thermo-acidophilic bacteria (Alicyclobacillus spp.) in 2019.

  • juice and juice-related products and their ingredients intended for human consumption
  • environmental samples including process water in the area of juice, juice-related production, and handling
  • other beverages not containing juices and their ingredients including syrups

This third edition from 2019 cancels and replaces the second edition of IFU Method No. 12: 2007, which has been technically revised. The main changes introduced in the third version, compared to IFU Method No. 12:2007, are considered as major.

11-minute talk: The new IFU Method No. 12:2019

Dr. Andreas Bubert explains the Alicyclobacillus spp. test procedure according to the new IFU Method No. 12:2019.

 

Procedure A:
Direct enumeration by pour plate technique acc. new IFU Method No. 12:2019

Procedure B:
Direct enumeration by filtration technique acc. IFU Method No. 12:2019

Procedure C:
Detection of Alicyclobacillus spp. by enrichment acc. new IFU Method No. 12:2019

 

GranuCult® granulated media

As a worldwide leading provider of a broad range of granulated and ready-to-use culture media for food, beverage and water microbiology, we closely track and support the development of relevant standards aimed at increasing consumer confidence and safety.

Our GranuCult® granulated media, Readybag® pre-weighed granulated media in ready-to-use bags, ReadyPlate™ and ReadyTube® ready-to-use media are all compliant with EN ISO 11133:2014. All information regarding compliance with additional reference standards are displayed on the product label and in the product’s technical information available on our website.

Compliance to New IFU Method No. 12:2019

Alicyclobacillus acidoterrestris DSM 2498 on BAT agar

We have implemented all the requirements described in the new IFU Method No. 12:2019. For more information, please visit SigmaAldrich.com/Beverages-Microbiology. The following culture media and accessories described in the new IFU Method No. 12:2019 are available:

Material Table

Product Pack size Catalog No.
GranuCult® BAT broth acc. IFU Method No. 12 500 g 107993
GranuCult® BAT agar acc. IFU Method No. 12 500 g 107994
GranuCult® Plate Count agar acc. ISO 4833, ISO 17410 and FDA-BAM 500 g 105463
ReadyPlate™ 55 Plate Count Agar 20x 55 mm plates 146763
Cellulose mixed ester filter: S-Pak® filters 0.45 µm, 47 mm, white gridded
600 individually sealed filters, sterile HAWG047S6
Cellulose mixed ester filter: EZ-Pak® filters 0.45 µm, 47 mm, white gridded 4 bands of 150 sterile filters EZHAWG474
EZ-Pak® Dispenser Curve   EZCURVE01
EZ-Stream® Vacuum Pump   EZSTREAM1
EZ-Fit® Manifold base, 1-place, stainless steel   EZFITBASE1
EZ-Fit® Manifold base, 1-place, stainless steel   EZFITBASE3
EZ-Fit® Manifold base, 1-place, stainless steel   EZFITBASE6