ISO 6579-1 for Salmonella Detection in the Food Chain

The International Organization for Standardization (ISO) published the new EN ISO 6579-1 standard in 2017, which specifies a horizontal method for the detection of Salmonella spp. in the food production chain.

Like the preceding ISO 6579:2002+Amd 1:2007, the latest ISO 6579-1:2017 covers the testing of products intended for human consumption, animal feeding and environmental samples in food production and handling.

In addition, EN ISO 6579-1:2017 now includes testing of milk and milk products as well as samples from the primary production stage, such as animal feces, dust and boot socks.

Procedure for Salmonella detection according to EN ISO 6579-1

Procedure steps for Salmonella detection according to new EN ISO 6579-1

EN ISO 6579-1 at a Glance

  • Part one (detection) of three-part EN ISO 6579 replaces EN ISO 6579:2004+Amd 1:2007
  • EN ISO 6785:2007 (dairy products) method details now incorporated
  • Samples from primary production stage added to scope
  • Incubation temperature range for non-selective media extended to 34° – 38°C
  • Choice of RVS or MSRV agar for selective enrichment of food, animal feed and environmental samples
  • Directions for choice of second isolation medium in addition to XLD agar
  • Only one suspect colony needed for confirmation (if negative, 4 more to be tested)
  • Optional: biochemical confirmation on well-isolated colony direct from selective plate
  • β-galactosidase and indole tests now optional, Voges-Proskauer reaction omitted
  • Detection of Salmonella Typhi and S. Paratyphi described • Performance testing for the culture media in-depth  

As a worldwide leading provider of a broad range of dehydrated granulated and ready-to-use culture media for food, beverages and water microbiology, we closely track and supports the development of relevant standards aimed at increasing consumer confidence and safety.

Our GranuCult™ granulated media, Readybag® pre-weighed granulated media in ready-to-use bags, ReadyPlate™ & ReadyTube™ ready-to-use media are all compliant with EN ISO 11133:2014. All information regarding compliance with additional reference standards are displayed on the product label and in the product’s technical information available on our website.  

Compliance to EN ISO 6579-1

We have implemented all the requirements described in EN ISO 6579-1. The following culture media and accessories described in ISO 6579-1 are available:  

Ordering Information

Product Pack Size Catalog No. Other Pack Sizes Available
GranuCult™ Buffered Peptone Water acc. ISO 6579, ISO 21528, ISO 22964, FDA-BAM and EP 500 g 1.07228.0500 5 kg, 25 kg
Readybag® Buffered Peptone Water acc. ISO 6579, ISO 21528, ISO 22964, FDA-BAM and EP, 5.7 g, irradiated 60 bags 1.02448.0060 60 bags x 29 g,
      35 bags x 86 g
ReadyTube™ 225 BPW ISO 6579, ISO 6887, ISO 21528, ISO 11290, FDA-BAM and EP 6 x 225 mL 1.46404.0006 6 x 1000 mL,
      20 x 9 mL
GranuCult™ RVS (RAPPAPORT-VASSILIADIS-Soya) Broth (Base) acc. ISO 6579 500 g 1.07700.0500  
Novobiocin Sodium Salt 1 g N6160-1-G 5 g, 25 g
ReadyTube™ 10 RVS Broth ISO 6579 20 x 10 mL 1.46694.0020  
GranuCult™ MKTTn (MULLER KAUFFMANN Tetrathionate Novobiocin) Broth (Base) acc. ISO 6579 500 g 1.05878.0500  
Potassium Iodide 250 g 1.05043.0250  
Iodine resublimed 100 g 1.04761.0100  
MSRV (Modified Semi-solid RAPPAPORT-VASSILIADIS) Medium (Base) acc. ISO 6579 500 g 1.09878.0500  
MSRV Selective Supplement 10 x 1 vial 1.09874.0010  
ReadyTube™ 12 MSRV Medium ISO 6579 100 x 12 mL 1.46622.0100  
GranuCult™ XLD (Xylose Lysine Deoxycholate) agar acc. ISO 6579 500 g 1.05287.0500  
ReadyPlate™ XLD Agar ISO 6579 20 x 90 mm 1.46751.0020  
RAMBACH® Agar 4 x 250 mL 1.07500.0001 4 x 1000 mL, 1 x 50 L
RAMBACH® Agar ready-to-use 20 x 90 mm 1.46719.0020 100 x 90 mm
GranuCult™ Nutrient agar acc. ISO 6579, ISO 10273 and ISO 21528 500 g 1.05450.0500  
Bismuth Sulfite Agar acc. WILSON-BLAIR 500 g 1.05418.0500  
Triple Sugar Iron Agar 500 g 1.03915.0500  
Urea Agar (Base) acc. CHRISTIANSEN 500 g 1.08492.0500  
Urea GR for Analysis ACS, Reagent Ph Eur 500 g 1.08487.0500  
MR-VP (Methyl Red-VOGES-PROSKAUER) Broth 500 g 1.05712.0500  
KOVAC’S Indole Reagent 100 mL 1.09293.0100